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Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients

World Journal of Gastroenterology 2011年第6期17卷 809-816页

作者： Hong shen Ying Yuan Han-guang Hu Xiao-Xian Ye Mo- Dan li Xian Zhong Wei-Jia fang Shu Zheng Department of Oncology Second Affiliated Hospital Zhejiang University College of Medicine Hangzhou 310009 Zhejiang Province China Department of Internal Medicine Bin Jiang Hospital Hangzhou 310052 Zhejiang Province China Department of Medical Oncology First Affiliated Hospital Zhejiang University College of Medicine Hangzhou 310003 Zhejiang Province China KeyLaboratory of Cancer Prevention and Intervention of Ministry of Education Cancer Institute Second Affiliated Hospital Zhejiang University College of Medicine Hangzhou 310009 Zhejiang Province China

AIM:To identify and assess mutations in the K-ras and BRAF genes in a cohort of Chinese patients with colorectal cancer (CRC) for their association with various clinicopathological parameters and ***:Genomic DNA was isolated from frozen *** analysis was conducted to detect mutations in the K-ras (codons 12,13,and 61) and BRAF genes (codon 600).Statistical analysis was carried out using SPSS-15.0 ***:Among the 118 colorectal cancer patients,we detected 41 (34.7%) mutations in the K-ras *** frequencies at codon 12 and codon 13 were 23.7% (28/118) and 10.2% (12/118),*** one patient harbored a point mutation at codon 61 (0.8%,1/118).Gender was the only factor that showed an obvious relationship with K-ras gene mutation (female 44.7% vs male 28.2%,P=0.037).Other clinicopathological features,such as age,location of the tumor,tumor differentiation,Tumor,Node and Metastases classification,and the Union for International Cancer Control staging,showed no positive relationship with K-ras gene *** significant correlation was observed between the presence of K-ras mutations (codons 12,13,and 61) and the survival of the *** mutations were rare,and only two patients (1.7%) harbored a detectable mutation at codon ***:K-ras gene mutation is a common event in our 118 Chinese CRC patients,with an obvious relationship with ***,it seems not to be an independent prognostic factor in CRC *** BRAF gene is rarely mutated in Chinese CRC patients.

关键词： K-ras BRAF Colorectal cancer Mutation

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涤纶全拉伸丝袜带染色质量影响因素探讨

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合成纤维 2024年第4期53卷 9-12页

作者：陶雅萍沈勤芳陈雄冯斌梁松华崔利新凤鸣集团股份有限公司浙江桐乡314500 嘉兴大学材料与纺织工程学院浙江嘉兴314000

为提升涤纶全拉伸丝(FDY)袜带染色质量,提高染色不良丝检出率,在RS-III型喷射式染色机固定程序条件下,通过控制单一变量进行试验,试验结果表明,在使用分散深蓝ER-FS 200%染料、染色温度99℃、染料质量分数1.0%、染色浴比1∶40的条件下,... 详细信息

为提升涤纶全拉伸丝(FDY)袜带染色质量,提高染色不良丝检出率,在RS-III型喷射式染色机固定程序条件下,通过控制单一变量进行试验,试验结果表明,在使用分散深蓝ER-FS 200%染料、染色温度99℃、染料质量分数1.0%、染色浴比1∶40的条件下,涤纶FDY袜带染色不良丝检出率较高,更有利于判色。

关键词：涤纶FDY 袜带染色影响因素

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Tributylphosphine-catalyzed reaction of ethanethiol with alkynyl ketones

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Chinese Chemical Letters 2011年第4期22卷 397-400页

作者： shen Zhao Qing Fa Zhou Jia Zhi liu Wei fang Tang tao Lu Department of Organic Chemistry China Pharmaceutical UniversityNanjing 210009China Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical University Ministry of EducationNanjing 210009China

A stereoselective and effective method for the synthesis of vinyl thioethers has been *** method is based on the Michael addition of ethanethiol to various alkynyl ketones using 10 mol%of tributylphosphine as *** of alkynyl ketones react with ethanethiol in this system to yield mainly Z-isomer of vinyl thioether adducts,only in one case mainly E-isomer of vinyl thioether adducts was observed.

关键词： Tributylphosphine Synthesis Alkynyl ketones Michael addition Vinyl thioether

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Cupping Therapy for Diseases: An Overview of Scientific Evidence from 2009 to 2019

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Chinese Journal of Integrative Medicine 2021年第5期27卷 394-400页

作者： WANG Song-zi LU yong-hui WU Min CHEN Ke-ji liu Yue liu Long-tao Department of Acupuncture and Moxibustion Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China General Department Guang’anmen HospitalChina Academy of Chinese Medical ScienceBeijing 100053China Department of Cardiovascular Diseases Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China

Cupping therapy has been accepted worldwide,and many studies have been conducted to reveal its curative effects and *** comprehensively evaluate the effect of cupping therapy,database including China Network Knowledge Infrastructure(CNKI),Chinese Scientific Journal Database VIP,Wan fang Database,Chinese Biomedicine(CBM),Pub Med and Web of Science were searched from 2009–*** summarized all the meta-analyses,randomized controlled trials,clinical trials and the mechanisms studies of cupping therapy in the previous 10 years,hoping to provide a reference for the clinical applications and studies.

关键词： cupping overview clinical trial efficacy mechanism non-drug therapy

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First-in-human evaluation of an independently developed Chinese robot-assisted system for percutaneous coronary intervention

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Journal of Geriatric Cardiology 2022年第10期19卷 743-752页

作者： guang-Yao ZHAI Zheng CHEN Rui-fang liu yong-He GUO Jian-Long WANG Tie-Nan SUN Jing XIE tao HUANG Yu-Jie ZHOU Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of Heart Lung and Blood Vessel DiseaseBeijing Key Laboratory of Precision Medicine of Coronary Atherosclerotic DiseaseClinical Center for Coronary Heart DiseaseCapital Medical UniversityBeijingChina Beijing WeMed Medical Equipment Co. Ltd.BeijingChina

BACKGROUND Several studies have proved the safety and feasibility of robot-assisted percutaneous coronary intervention(PCI)in reducing the occupational hazards of interventionists while achieving precision ***,an independently developed robot-assisted system for PCI in China has not yet *** study aimed to evaluate the safety and feasibility of a robot-assisted system for elective PCI in *** This preclinical trial included 22 experimental pigs and preliminarily supported the safety and feasibility of the ETcath200 robot-assisted system for ***,eleven patients with coronary heart disease who met the inclusion criteria and had clinical indications for elective PCI were *** was performed using a robot-assisted *** primary outcomes were clinical success(defined as visual estimated residual stenosisduring hospitalization and within 30 days after PCI)and technical success(defined as the ability to use the robot-assisted system to complete PCI successfully without conversion to the traditional manual PCI).RESULTS Eleven patients were included in this clinical trial.A drug-eluting stent with a diameter of 3 mm(interquartile range:2.75–3.5 mm)and a length of 26 mm(interquartile range:22–28 mm)was deployed in all *** clinical success rate was 100%,with no PCI-related complications and no in-hospital or 30-day major adverse cardiovascular events,and the technical success rate was 100%.CONCLUSIONS The results strongly suggest that the use of the independently developed robot-assisted system in China for elective PCI is feasible,safe,and effective.

关键词： coronary robot system

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Detachment of esophageal carcinoma cells from extracellular matrix causes relocalization of death receptor 5 and apoptosis

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World Journal of Gastroenterology 2009年第7期15卷 836-844页

作者： guang-Chao liu Jun Zhang Shi-Gui liu Rong Gao Zhang-Fu Long Ke tao Yuan-fang Ma Key Laboratory of Bio-resource and Eco-environment of China Ministry of Education College of Life Sciences of Sichuan University Chengdu 610064 Sichuan Province China Henan Provincial Key Laboratory for Natural Drug and Immune Engineering Henan University Kaifeng 475004 Henan Province China Institute of Immunology Medical College Henan University Kaifeng 475004 Henan Province China

AIM： To investigate the effect of detachment of esophageal cancer cells from extracellular matrix on the localization of death receptor 5 （DR5） and apoptosis. METHODS： Anchorage-dependent EC9706 cells of esophageal squamous cell carcinoma were pretreated or not treated with brefeldin A. Detached cells were harvested by ethylenediaminetetraacetic acid digestion. Expression and localization of DR5 in these cells were determined by immunocytochemical and immunofluorescence assays, as well as flow cytometry analysis. Apoptosis of EC9706 cells was detected by flow cytometry after stained with fluorescein isothiocyanate-labeled annexin V/propidium iodide. Activation of caspase 8 was detected by Western blot analysis. RESULTS： Immunocytochemical assay indicated that DR5 was predominantly perinuclear in adherent cells but was mainly localized in cell membrane in detached cells. In addition, immunofluorescence assay also confirmed the above-mentioned results, and further demonstrated that DR5 was present in the form of coarse granules in detached cells, but in the form of fine granules in adherent cells. Cytometry analysis revealed higher levels of DR5 expression on the surfaces of brefeldin-A-untreated cells than on the surfaces of brefeldin-A-treated cells, but brefeldin A treatment did not affect the total DR5 expression levels. Moreover, nocodazole did not influence the extracelluar DR5 expression levels in EC9706 cells. Apoptosis assay revealed that detached cells were more sensitive to DR5 antibody-induced apoptosis than adherent ceils. Western blotting showed that caspase 8 was activated in temporarily detached cells 4 h earlier than in adherent cells. CONCLUSION： Progress from adhesion to detachment of EC9706 cells causes DR5 relocalization, and promotes cytoplasmic translocation of DR5 to cell surfaces via a Golgi-dependent pathway. Moreover, it might also result in DR5 aggregation to render apoptosis of detached cells.

关键词： Translocation of death receptor 5 Celldetachment Esophageal carcinoma Anoikis Apoptosis

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Sedimentary characteristics of microbialites influenced by volcanic eruption: a case study from the Lower Cretaceous Shipu Group in Zhejiang Province, East China

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Journal of Palaeogeography 2020年第2期9卷 232-245页

作者： Xiao-fang Wang Xiu-Cheng Tan Shao-Nan Zhang An-Jiang shen Chang li guang Hu Xin Wang Zi-liao Chen li-Yin Pan Jie Zhang Wei Chen State Key Laboratory of Oil and Gas Geology and Exploitation Southwest Petroleum UniversityChengdu 610500Sichuan ProvinceChina School of Geoscience and Technology Southwest Petroleum UniversityChengdu 610500Sichuan ProvinceChina Key Laboratory of Carbonate Reservoirs China National Petroleum Corporation(CNPC)Hangzhou 310023Zhejiang ProvinceChina PetroChina Hangzhou Research Institute of Geology Hangzhou 310023Zhejiang ProvinceChina.

This study describes a sequence of microbialites and volcanics of the Lower Cretaceous Shipu Group, an example of microbialites influenced by volcanic activity. It is located at Shipu town in eastern Zhejiang Province on the coast of southeastern China. Based on macroscopic outcrop observations, microscopic examination of thin sections,electron probe microanalysis(EPMA), field emission scanning electron microscopy(FESEM) imaging analysis, and energy dispersive X-ray spectrometry(EDS) analysis, nine microbialite–tuffite assemblages have been recognized in the section. Their thickness increased gradually upwards as volcanism decreased. There are ooids, bioclastic grains,intraclasts and tuffaceous grains in the grain shoal with local dolomitization. Above the grain shoal, microbial reefs develop either individually or conjoining with adjacent ones, and consist of stromatolites and serpulid tubes with common recrystallization. Tubes of serpulids are calcified and the tube wall is micrite. The tube and intertube parts are filled by sparry calcite. Colonial serpulids are surrounded by microbes to form stromatolites. Black layers of stromatolites contain many calcite crystals with fan-shaped growth pattern and preserved organic matter. Microbes are so well preserved in crystal lattices that the original microstructure of the microbes can be clearly observed by FESEM imaging analysis. Microbial reefs develop at a local high point near or above fair-weather wave-base where waves removed fine volcanic ashes. Interreef deposits are coarse tuffite due to physical differentiation. Volcanic activity could provide rich nutrition for microbes, but too much fine volcanic ash inhibits microbial growth. As a result, a moderate supply of volcanic ash favors the development of microbialites.

关键词： Microbialite Stromatolites Tuffite Lower Cretaceous Shipu Group

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基于无源移频MIMO实现传统单路DAS网络的性能提升研究

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电信工程技术与标准化 2024年第4期37卷 60-63页

作者：陈辉王芳陈光洪康迎冰池敬涛中国移动通信集团设计院有限公司广东分公司广州510630 中国移动通信集团广西有限公司南宁530028

为了验证无源移频MIMO技术可有效提升5G室内网络性能,本文研究了在传统单路DAS场景下,通过进行无源移频MIMO改造提升5G上下行速率的试点情况。此研究给后续如何在现有5G室内网络低成本高效率提升5G网络性能提供了参考。

关键词：无源MIMO 单路DAS 网络性能

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Cryo-ET bridges the gap between cell biology and structural biophysics

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Chinese Physics B 2018年第6期27卷 62-71页

作者： Xiao-fang Cheng Rui Wang Qing-tao shen iHuman Institute ShanghaiTech University Shanghai 20121 O China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266237 China School of life Science and Technology ShanghaiTech University Shanghai 201210 China

Cryo-electron tomography（cryo-ET） is a cutting-edge technology providing three-dimensional in situ ultra-structural information of macromolecular machineries, organelles, and eukaryotic cells in their native environment at an unprecedented level of detail. Cryo-ET enables the direct observation of dynamic macromolecular architectures of bio-samples in their naturally occurring physiological state, without any harmful artifacts introduced by heavy metal staining, dehydration, and chemical fixation, which occur in traditional transmission electron microscopy. Over decades, cryo-ET has been providing insights into numerous aspects of cellular biology by revealing the pristinely preserved ultra-structures of different cellular components comprising the crowded and complex environment of the cell, thus, bridging the gap between cellular biology and structural biophysics. In this paper, we review the fundamentals of this technique, its recent advances in optics, detection devices, and computational algorithms. The enhancement of our understanding of structural cellular biology by combining these improvements, when integrated with other methods, such as cryo-focused ion beam milling,correlative light and electron microscopy, is discussed via a few examples from research groups worldwide. We also believe that cryo-ET applications in cell biology continue to provide fundamental insights into the field, revolutionizing structural biology itself.

关键词： sub-tomogram averaging segmentation direct detection phase plate

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心内膜心肌活检在心脏疾病诊断与评估中的应用

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心脏杂志 2024年第3期36卷 333-336页

作者：李通政王正阳孙芳芳胡洸瑜陶凌张富洋空军军医大学第一附属医院心血管内科陕西西安710032 空军军医大学基础医学院学员六大队陕西西安710032

心内膜心肌活检(endomyocardial biopsy,EMB)是一种通过侵入性手术获取患者心内膜心肌组织的方法,最初主要用于监测异体心脏移植术后排斥反应,目前已发展为明确诊断和评估心肌炎、心肌病、药物相关的心脏毒性、淀粉样变性、贮存性疾病... 详细信息

心内膜心肌活检(endomyocardial biopsy,EMB)是一种通过侵入性手术获取患者心内膜心肌组织的方法,最初主要用于监测异体心脏移植术后排斥反应,目前已发展为明确诊断和评估心肌炎、心肌病、药物相关的心脏毒性、淀粉样变性、贮存性疾病及心脏肿瘤等多种心脏疾病的重要手段。本文就EMB的发展、临床应用及注意事项进行总结。

关键词：心脏疾病心内膜心肌活检临床方法

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