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高寒草地耐低温植物根际促生菌的筛选鉴定及特性研究

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草业学报 2022年第8期31卷 178-187页

作者：刘晓婷姚拓甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心甘肃兰州730070

为获得优良植物根际促生菌(PGPR)并明确其促生特性,以红原地区广泛分布的3种高寒草地优势植物为材料,采用选择性培养基从3种高寒植物根际分离、筛选具有固氮、溶磷、分泌植物激素能力的耐低温植物根际促生菌,并进行16S rRNA分子生物学... 详细信息

为获得优良植物根际促生菌(PGPR)并明确其促生特性,以红原地区广泛分布的3种高寒草地优势植物为材料,采用选择性培养基从3种高寒植物根际分离、筛选具有固氮、溶磷、分泌植物激素能力的耐低温植物根际促生菌,并进行16S rRNA分子生物学鉴定。结果表明:毛稃羊茅、洽草、紫穗鹅观草植物根际分布着大量根际细菌,且PGPR在根际不同部位的数量呈现出根系表面(RP)>根表土壤(RS)>根内(HP)的分布规律。经筛选共获得76株PGPR菌株,其中固氮菌30株,其固氮酶活性为124.61~311.04 nmol C_(2)H_(4)·h^(-1)·mL^(-1);溶解无机磷菌株24株,溶解有机磷菌株22株,溶磷量分别为249.85~558.07μg·mL^(-1)和52.25~158.77μg·mL^(-1)。挑选固氮酶活性较高、溶磷能力较强的27株PGPR菌株进行分泌植物激素能力测定,26株菌株具有分泌赤霉素(GA_(3))的能力,分泌量为0.60~52.91μg·mL^(-1),分泌吲哚-3-乙酸(IAA)菌株18株,分泌量为0.11~1.53μg·mL^(-1),分泌玉米素(t-Z)含量均小于0.31μg·mL^(-1)。筛选出综合性能优良的菌株14株,经鉴定分别属于假单胞菌属、不动杆菌属、假节杆菌属、贪噬菌属。研究结果可为研制适合高寒地区植被恢复的微生物肥料提供宝贵的菌种资源及理论基础。

关键词：植物根际促生菌固氮菌溶磷菌植物激素耐低温菌

An efficient stiffness analysis model based on shear deformation theory for flexible skin shear variable-sweep wing

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Chinese Journal of Aeronautics 2024年第10期37卷 445-458页

作者： Yue BAI Guang YANG Hong xiao Hongwei GUO Rongqiang liu Bei liu State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China China Aerodynamics Research and Development Center Institute of High-Speed AerodynamicsMianyang 621000China

Fixed-wing aircraft cannot maintain optimal aerodynamic performance at different flight *** a type of morphing aircraft,the shear variable-sweep wing(SVSW)can dramatically improve its aerodynamic performance by altering its shape to adapt to various flight *** order to achieve smooth continuous shear deformation,SVSW’s skin adopts a flexible composite skin design instead of traditional aluminum alloy ***,this also brings about the non-linear difficulty in stiffness modeling and *** this research,a new SVSW design and efficient stiffness modeling method are *** on shear deformation theory,the flexible composite skin is equivalently modeled as diagonally arranged nonlinear springs,simulating the elastic force interaction between the skin and the *** shear loading tests of flexible composite skin,the accuracy of this flexible composite skin modeling method is *** SVSW stiffness model was established,and its accuracy was verified through static loading *** effects of root connection,sweep angles,and flexible composite skin on the SVSW stiffness are ***,considering three typical flight conditions of SVSW:low-speed flow(Ma=0.3,Re=5.8210^(6)),transonic flow(Ma=0.9,Re=3.4410^(6)),and supersonic flow(Ma=3,Re=7.5110^(6)),the stiffness characteristics of SVSW under flight conditions were *** calculated results guide the application of SVSW.

关键词： Shear variable-sweep wing(SVSW) Flexible composite skin Morphing mechanism Stiffness analysis Loading tests Structure

Recent advances in vacancy engineering for reliable lithiumsulfur batteries

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Rare Metals 2024年第2期43卷 455-477页

作者： Wen-Dong liu xiao Tang Jun-An Feng Chao-Yue Zhang Hao liu Chuan Shi xiao-Xian Zhao Jian-Jun Song College of Physics School of Chemistry and Chemical EngineeringQingdao UniversityQingdao266071China Faculty of Science Centre for Clean Energy TechnologySchool of Mathematical and Physical SciencesUniversity of Technology SydneySydneyNSW2007Australia Department of Chemistry College of ScienceHebei Agricultural UniversityBaoding071001China

The high-energy-density and low-cost features endow lithium-sulfur batteries with broad application ***,many drawbacks,especially the detrimental shuttle effect,have hindered the further development of *** response,a lot of new structures have been applied to suppress the shuttle effect and promote the development of ***,vacancy engineering has gained the attention of researchers due to its unique electronic *** review aims to analyze the application of vacancy engineering in ***,the electrochemistry of LSBs has been systematically discussed and the existing challenges as well as improvement tactics of LSBs have also been ***,the preparation methods and characterization technologies of various vacancies are summarized,including oxygen vacancies,sulfur vacancies,selenium vacancies,other anion vacancies,cation vacancies,*** latest applications of vacancy engineering in LSBs are also summarized in this ***,some prospects and insights for further investigation and practical application of vacancy engineering in LSBs are put forward.

关键词： Lithium-sulfur batteries(LSBs) Defects Vacancy engineering Electronic structure Shuttle effect

The Trend of Smoking-Related Cognition and Its Association with Smoking Behaviors Among Junior High School Students—China,2013–2021

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China CDC weekly 2024年第22期6卷 503-508页

作者： Wenzhao liu Xinying Zeng Qi Su Lin xiao Shiwei liu Tobacco Control Office Chinese Center for Disease Control and PreventionBeijingChina

What is already known about this topic?In 2013,31.61%of students perceived quitting smoking as difficult,61.73%considered smoking less attractive,and 73.89%believed that secondhand smoke is definitely harmful to *** is added by this report?The percentage of students who perceived quitting smoking as difficult increased from 31.61%in 2013 to 38.83%in 2021,while the percentage of students who found smoking less attractive rose from 61.73%to 69.40%.Conversely,there was a decrease in the percentage of students who perceived secondhand smoke as harmful,from 73.89%to 68.46%.An increased awareness of the hazards of secondhand smoke was associated with a reduction in smoking *** are the implications for public health practice?It is imperative to enhance health education efforts that aim to raise awareness of the hazards of secondhand smoke.

关键词： smoke attractive Junior

KSKV:Key-Strategy for Key-Value Data Collection with Local Differential Privacy

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Computer Modeling in Engineering & Sciences 2024年第6期139卷 3063-3083页

作者： Dan Zhao Yang You Chuanwen Luo ting Chen Yang liu Artificial Intelligence Development Research Center Institute of Scientific and Technical Information of ChinaBeijing100038China Industry Development Department NSFOCUS Inc.BeijingChina School of Information Science and Technology Beijing Forestry UniversityBeijing100083China School of Computer Science and Engineering University of Electronic Science and Technology of ChinaChengdu610054China Institute of Computing Technology China Academy of Railway Sciences Corporation LimitedBeijing10081China

In recent years,the research field of data collection under local differential privacy(LDP)has expanded its focus fromelementary data types to includemore complex structural data,such as set-value and graph ***,our comprehensive review of existing literature reveals that there needs to be more studies that engage with key-value data *** studies would simultaneously collect the frequencies of keys and the mean of values associated with each ***,the allocation of the privacy budget between the frequencies of keys and the means of values for each key does not yield an optimal utility *** the importance of obtaining accurate key frequencies and mean estimations for key-value data collection,this paper presents a novel framework:the Key-Strategy Framework forKey-ValueDataCollection under ***,theKey-StrategyUnary Encoding(KS-UE)strategy is proposed within non-interactive frameworks for the purpose of privacy budget allocation to achieve precise key frequencies;subsequently,the Key-Strategy Generalized Randomized Response(KS-GRR)strategy is introduced for interactive frameworks to enhance the efficiency of collecting frequent keys through group-anditeration *** strategies are adapted for scenarios in which users possess either a single or multiple key-value ***,we demonstrate that the variance of KS-UE is lower than that of existing *** claims are substantiated through extensive experimental evaluation on real-world datasets,confirming the effectiveness and efficiency of the KS-UE and KS-GRR strategies.

关键词： Key-value local differential privacy frequency estimation mean estimation data perturbation

Tailoring electron vortex beams with customizable intensity patterns by electron diffraction holography

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Opto-Electronic Advances 2024年第2期7卷 14-21页

作者： Pengcheng Huo Ruixuan Yu Mingze liu Hui Zhang Yan-qing Lu ting Xu National Laboratory of Solid-State Microstructures and Collaborative Innovation Center of Advanced Microstructures Nanjing UniversityNanjing 210093China College of Engineering and Applied Sciences Key Laboratory of Intelligent Optical Sensing and Manipulation and Jiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjing 210093China

An electron vortex beam(EVB) carrying orbital angular momentum(OAM) plays a key role in a series of fundamental scientific researches, such as chiral energy-loss spectroscopy and magnetic dichroism spectroscopy. So far, almost all the experimentally created EVBs manifest isotropic doughnut intensity patterns. Here, based on the correlation between local divergence angle of electron beam and phase gradient along azimuthal direction, we show that free electrons can be tailored to EVBs with customizable intensity patterns independent of the carried OAM. As proof-of-concept, by using computer generated hologram and designing phase masks to shape the incident free electrons in the transmission electron microscope, three structured EVBs carrying identical OAM are tailored to exhibit completely different intensity patterns. Furthermore, through the modal decomposition, we quantitatively investigate their OAM spectral distributions and reveal that structured EVBs present a superposition of a series of different eigenstates induced by the locally varied geometries. These results not only generalize the concept of EVB, but also demonstrate an extra highly controllable degree of freedom for electron beam manipulation in addition to OAM.

关键词： electron vortex beam orbital angular momentum diffraction holography

Generation of mitochondrial replacement monkeys by female pronucleus transfer

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Zoological Research 2024年第2期45卷 292-298页

作者： Chun-Yang Li Xing-Chen liu Yu-Zhuo Li Yan Wang Yan-Hong Nie Yu-ting Xu xiao-Tong Zhang Yong Lu Qiang Sun Institute of Neuroscience Center for Excellence in Brain Science and Intelligence TechnologyState Key Laboratory of NeuroscienceChinese Academy of SciencesShanghai 200031China Key Laboratory of Genetic Evolution and Animal Models Chinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe *** replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA *** pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously *** this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear *** individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during *** successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.

关键词： Non-human primates Mitochondrial replacement Female pronuclear transfer

Moderate expression of CD39 in GPC3-CAR-T cells shows high efficacy against hepatocellular carcinoma

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Frontiers of Medicine 2024年第4期18卷 708-720页

作者： Fan Zou Jialiang Wei Jialang Zhuang Yafang liu Jizhou Tan Xianzhang Huang ting liu Guangdong Cardiovsacular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical ScienceGuangzhou510080China The Second Clinical Medical College Guangzhou University of Chinese MedicineDepartment of Laboratory Medicine/State Key Laboratory of Traditional Chinese Medicine SyndromeGuangdong Provincial Hospital of Chinese MedicineGuangzhou510120China Department of Medical Oncology The First Affiliated Hospital of Guangxi Medical UniversityNanning530021China Department of Stomatology the First Affiliated HospitalSun Yat-sen UniversityGuangzhou510080China Medical Research Institute Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Science)Southern Medical UniversityGuangzhou510080China School of Food and Drug Shenzhen Polytechnic UniversityShenzhen518055China Shenzhen Second People’s Hospital First Affiliated Hospital of Shenzhen UniversityShenzhen518055China

CD39 serves as a crucial biomarker for neoantigen-specific CD8^(+)T cells and is associated with antitumor activity and ***,the relationship between CD39 expression levels and the function of chimeric antigen receptor T(CAR-T)cells remains *** study aimed to investigate the role of CD39 in the functional performance of CAR-T cells against hepatocellular carcinoma(HCC)and explore the therapeutic potential of CD39 modulators,such as mitochondrial division inhibitor-1(mdivi-1),or knockdown CD39 through short hairpin *** findings demonstrated that glypican-3-CAR-T cells with moderate CD39 expression exhibited a strong antitumor activity,while high and low levels of CD39 led to an impaired cellular *** modulating the proportion of CD39 intermediate(CD39^(int))-phenotype CAR-T cells such as mdivi-1 and CD39 knockdown enhanced and impaired T cell function,*** combination of mdivi-1 and CD39 knockdown in CAR-T cells yielded the highest proportion of infiltrated CD39^(int)CAR-T cells and demonstrated a robust antitumor activity in *** conclusion,this study revealed the crucial role of CD39 in CAR-T cell function,demonstrated the potential therapeutic efficacy of combining mdivi-1 with CD39 knockdown in HCC,and provided a novel treatment strategy for HCC patients in the field of cellular immunotherapy.

关键词： CD39 CAR-T cells mdivi-1 hepatocellular carcinoma antitumor activity

幻想性与审美追求——浅析舒曼钢琴变奏曲中的幻想性

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山东大学学报（哲学社会科学版） 2007年第5期 158-160页

作者：刘晓婷中国石油大学人文社会科学部北京102249

舒曼的音乐深受德国早期文学家的影响,其钢琴变奏曲于同时期的浪漫派文学作品表现着相同的特征———富于幻想。舒曼对音乐的追求体现在他的音乐创作中,而其变奏思维与音乐的幻想性紧密相连,充分展现了他对音乐的审美追求,而这种追求又... 详细信息

舒曼的音乐深受德国早期文学家的影响,其钢琴变奏曲于同时期的浪漫派文学作品表现着相同的特征———富于幻想。舒曼对音乐的追求体现在他的音乐创作中,而其变奏思维与音乐的幻想性紧密相连,充分展现了他对音乐的审美追求,而这种追求又使舒曼钢琴变奏作品在幻想中表现深刻的思想,使音乐呈现出独特的艺术价值。

关键词：变奏曲变奏思维幻想性审美追求