检索结果-南通市图书馆

Effect of dietary supplementation with flavonoid from Scutellaria baicalensis Georgi on growth performance,meat quality and antioxidative ability of broilers

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第5期17卷 1165-1170页

作者： liao xiu-dong WEN Qian ZHANG Ling-yan LU Lin ZHANG Li-yang LUO Xu-gang Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural Sciences

An experiment was conducted to investigate the effect of dietary supplementation with flavonoid from Scutellaria baicalensis Georgi（SBGFN） as SBGFN-zinc（SBGFN-Zn） on growth performance, meat quality, immune responses and antioxidation of broilers. A total of 450 one-d-old Arbor Acres male broilers were randomly allocated to 5 treatments with 6 replicates of 15 birds per replicate for each treatment in a completely randomized design. Birds were fed a SBGFN-unsupplemented cornsoybean meal basal diet（control） or the basal diet supplemented with 60, 120, 180 or 240 mg SBGFN kg-（–1） from SBGFN-Zn for 42 d. Dietary SBGFN supplementation affected（P〈0.03） drip loss in thigh muscle, total superoxide dismutase（T-SOD） and glutathione peroxidase（GSH-Px） activity in liver of broilers at 42 d of age. Chicks fed the diets supplemented with 120, 180 and 240 mg SBGFN kg-（–1） had lower（P〈0.03） drip loss of thigh muscle than those fed the control diet. Chicks fed the diet supplemented with 180 mg SBGFN kg-（–1） had higher（P〈0.03） liver T-SOD and GSH-Px activity than those fed the diets supplemented with 0, 60 and 120 mg SBGFN kg-（–1）. The results from the present study indicate that dietary supplementation with 180 mg SBGFN kg-（–1） as SBGFN-Zn improved both meat quality and antioxidative ability of broilers.

关键词： flavonoid growth performance meat quality antioxidation

Manganese enhances the expression of the manganese superoxide dismutase in cultured primary chick embryonic myocardial cells

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第9期16卷 2038-2046页

作者： QIN Shi-zhen liao xiu-dong LU Lin ZHANG Li-yang XI Lin GUO Yan-li LUO Xu-gang Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural Sciences College of Animal Science and Technology Gansu Agricultural University Department of Animal Science North Carolina State University

In the present study, the effect of manganese（Mn） on antioxidant status and the expression of the manganese superoxide dismutase（MnSOD） gene in cultured primary myocardial cells collected from the chick embryos was investigated. The hypothesis that Mn supplementation would enhance the expression of MnSOD in cultured primary myocardial cells of chick embryos was tested. Eggs collected from Mn-depleted Arbor Acres laying breeder hens were incubated for 10 days and then myocardial cells were isolated and cultivated for 8 days. The embryonic myocardial cells on day 6 were treated with Mn in the cell culture medium at different time points when the proportion of cells showing spontaneous contraction was over 95% after the 3-day primary culture. A completely randomized design involving a 3 Mn levels（0, 0.5 and 1.0 mmol L^（-1））×3 incubation time points（12, 24 and 48 h） factorial arrangement of treatments（n=6） was used in the current experiment. The results showed that MnSOD activity and m RNA expression level were induced by Mn and increased with incubation time, which supported the hypothesis that Mn would enhance the expression of the MnSOD gene, and thus might protect myocardial cells from oxidative stress during the chick embryonic development.

关键词： manganese MnSOD expressions cultured primary myocardial cells chick embryos

Effect of in ovo zinc injection on the embryonic development, tissue zinc contents, antioxidation, and related gene expressions of broiler breeder eggs

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第3期17卷 648-656页

作者： SUN Xiao-ming liao xiu-dong LU Lin ZHANG Li-yang MA Qiu-gang XI Lin LUO Xu-gang Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences College of Animal Science and Technology China Agricultural University Department of Animal Science North Carolina State University

Two experiments were conducted to investigate the effect of in ovo zinc （Zn） injection on the embryonic development, tissue Zn contents, antioxidation and related gene expressions of fertilized eggs of Arbor Acres broiler breeders. Experiment 1 was conducted to determine an optimal embryonic age for early in ovo injection. A total of 720 fertilized eggs with similar weights were randomly allotted to 4 treatments with 6 replicates per treatment and 30 eggs per replicate in a completely randomized design. The eggs were injected with 0.1 mL sterilized water at 3, 6 and 9 embryonic days of incubation （E3, E6 and E9） or non-injection （the control）, respectively. The results from experiment 1 showed that E3 and E6 injections increased （P〈0.05） the embryonic mortalities, and decreased （P〈0.05） hatchabilities compared to the non-injected control, but no differences （P〉0.05） between E9 injection and the non-injected control were observed in either embryonic mortality or hatchability. The findings suggest that the E9 is the optimal embryonic age for early in ovo injection. In experiment 2, a total of 672 fertilized eggs with similar weights were randomly allocated to 7 treatments with 6 replicates per treatment and 16 eggs per replicate in a completely randomized design. The eggs were injected with 0 （the negative control）, 50, 100, 150, 200, or 250 μg Zn/egg as reagent grade ZnSO4.7H20 in a 0.1-mL solution, or non-injection （the positive control）, respectively at E9-10. The results from the experiment 2 demonstrated that no differences （P〉0.05） among 50 and 100 μg Zn/egg groups and the negative control were observed in the embryonic mortality and hatchability, however, the injection of 200 μg Zn/egg increased （P〈0.05） the embryonic mortality, and injections of 150 and 200 μg Zn/egg decreased （P〈0.05） hatchabilities compared with the controls. The embryonic tibia Zn contents at E20 were increased （P〈0.05） by injections of 150, 200 and 250μg Zn/egg. Zinc injection did not affe

关键词： in ovo injection zinc embryonic development gene expression broiler breeder egg

Effects of dietary graded levels of cinnamon essential oil and its combination with bamboo leaf flavonoid on immune function, antioxidative ability and intestinal microbiota of broilers

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第9期18卷 2123-2132页

作者： YANG Yun-feng ZHAO Lu-lu SHAO Yu-xin liao xiu-dong ZHANG Li-yang LU Lin LUO Xu-gang Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences

Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not *** experiment was conducted to investigate the effects of dietary graded levels of CEO and its combination with BLF on the growth performance,immune responses,antioxidative ability,and intestinal morphology and microbiota of broilers fed a corn-soybean meal basal diet.A total of 576 1-d-old Arbor Acres commercial male broilers were randomly allotted to 9 treatments with 8 replicates of 8 birds each in a completely randomized *** were fed on a basal corn-soybean meal diet(control,without plant extracts and antibiotics),or the basal diet supplemented with 50 mg of aureomycin kg^-1,50,100,200,400,or 800 mg of CEO kg^-1,a combination of 100 mg of CEO and 16.7 mg of BLF kg^-1,or a combination of 200 mg of CEO kg^-1 and 33.3 mg of BLF kg^-1 for 42 *** treatment affected(P0.16)on all other measured *** addition of 400 mg of CEO kg^-1 or a combination of 200 mg CEO kg^-1 and 33.3 mg BLF kg^-1 increased(P<0.02)serum IgM contents on d *** supplementation with 100 or 200 mg CEO kg^-1,or 50 mg aureomycin kg^-1 decreased(P<0.003)liver MDA contents on d *** addition,the supplement of 100 mg CEO kg^-1 increased(P<0.002)the Lactobacillus relative abundance in caecum on d 21 and Bifidobacterium relative abundance in caecum on d 42,and decreased(P<0.0001)*** relative abundance in caecum on d *** results indicated that dietary supplementation with CEO,an alternative to aureomycin,improved the immune status,antioxidantative ability and cecal microbiota of b

关键词： plant extracts immune responses antioxidative ability intestinal morphology cecal microbiota broilers

Effect of dietary calcium or phosphorus deficiency on bone development and related calcium or phosphorus metabolic utilization parameters of broilers from 22 to 42 days of age

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第11期19卷 2775-2783页

作者： YANG Yun-feng XING Guan-zhong LI Su-fen SHAO Yu-xin ZHANG Li-yang LU Lin LUO Xugang liao xiu-dong Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China College of Animal Science and Technology Hebei Normal University of Science and TechnologyQinhuangdao 066004P.R.China

This experiment was conducted to investigate the effect of dietary calcium(Ca)or phosphorus(P)deficiency on bone development and related Ca or P metabolic utilization parameters of broilers from 22 to 42 days of age based on our previous study,which indicated that dietary Ca or P deficiency impaired the bone development by regulating related Ca or P metabolic utilization parameters of broilers from 1 to 21 days of age.A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates in a completely randomized design,and fed the normal control and Ca-or P-deficient diets from 1 to 21 days of *** 22 days of age,the broilers were further fed the normal control diet(0.90%Ca+0.35%non-phytate P(NPP)),the P-deficient diet(0.90%Ca+0.18%NPP),the Ca-deficient diet(0.30%Ca+0.35%NPP)or the Ca and P-deficient diet(0.30%Ca+0.18%NPP),*** results showed that dietary Ca or P deficiency decreased(P<0.05)tibia bone mineral density(BMD),bone breaking strength(BBS),ash content,tibia ash Ca content and serum P content on days 28 and 42,but increased(P<0.05)tibia alkaline phosphatase(ALP)activity of broilers on day 42 compared with the control ***,the broilers fed the P-deficient diet had the lowest(P<0.05)tibia BMD,BBS,ash content,serum P content and the highest(P<0.05)serum Ca content on day 28 compared with those fed the Ca-deficient or Ca and P-deficient *** results from the present study indicated that the bone development and related Ca or P metabolic utilization parameters of broilers were the most sensitive to dietary P deficiency,followed by dietary Ca deficiency or Ca and P-deficiency;dietary Ca or P deficiency impaired the bone development possibly by regulating serum Ca and P contents as well as tibia Ca content and ALP activity of broilers from 22 to 42 days of age.

关键词： calcium deficiency phosphorus deficiency bone development metabolic utilization parameter broiler

Effect of the gene silencing of phosphorus transporters on phosphorus absorption across primary cultured duodenal epithelial cell monolayers of chick embryos

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第7期21卷 2076-2085页

作者： LI Ting-ting LU Na SHAO Yu-xin ZHANG Li-yang LU Lin LIU Zong-ping LUO Xu-gang liao xiu-dong Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China College of Veterinary Medicine Yangzhou UniversityYangzhou 225009P.R.China

The aim of the study was to investigate whether phosphorus(P) transporters, type IIb sodium-dependent phosphate cotransporter(NaP-IIb) and inorganic phosphate transporter 2(PiT2), were directly involved in P absorption across primary cultured duodenal epithelial cell monolayers of chick embryos. The siRNAs against NaP-IIb or PiT2 were designed, synthesized and transfected into primary cultured duodenal epithelial cells of chick embryos. Then, the inhibitory efficiency of siRNAs against NaP-IIb or PiT2 was analyzed, and the most efficacious siRNAs were selected to be used for subsequent P absorption experiments. Briefly, primary cultured duodenal epithelial cells of chick embryos were transfected with either NaP-IIb or PiT2 siRNAs and grown in confluent monolayers on transwell plates. The untransfected or transfected cell monolayers were then incubated in an uptake medium containing 0 or 0.25 mmol L^(–1) of P as KH_(2) PO_(4) to measure the P absorption across duodenal epithelial cell monolayers. The results showed that among the siRNAs designed, si-1372 and si-890 were demonstrated to be the most effective in inhibiting the NaPIIb and PiT2 expressions, respectively. Supplemental P increased(P=0.065) the protein abundance of PiT2 and enhanced(P<0.0001) P absorption in primary cultured duodenal epithelial cell of chick embryos. Furthermore, NaPIIb silencing decreased(P=0.07) P absorption across duodenal epithelial cell monolayers, while PiT2 silencing had no effect(P=0.345). It is concluded that the NaP-IIb, but not PiT2, might be directly involved in the P absorption of chick duodenal epithelial cells.

关键词： broiler phosphorus transporter phosphorus absorption primary cultured duodenal epithelial cell

Effects of selenium source and level on growth performance, antioxidative ability and meat quality of broilers

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2021年第1期20卷 227-235页

作者： WANG Chuan-long XING Guan-zhong WANG Li-sai LI Su-fen ZHANG Li-yang LU Lin LUO Xu-gang liao xiu-dong Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao 066004P.R.China

This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg^–1. The results showed that broilers fed the Se-supplemented diets had higher(P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase(GSH-Px) activities in breast and thigh muscles on d 42, and lower(P<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde(MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher(P<0.05) p H value and lower(P<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg^–1 had lower(P<0.05) shear force in thigh muscle and higher(P<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg^–1. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg^–1 had higher(P<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg^–1, but no differences(P<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg^–1. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antio

关键词： selenium source growth performance meat quality antioxidative ability broiler

Kinetics of selenium absorption in ligated small intestinal loops of chicks

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第8期19卷 2095-2102页

作者： LIU Guo-qing ZHANG Shu-min AN Zhi-min FENG Yan-zhong dong Xue-yu LI Su-fen LU Lin ZHANG Li-yang WANG Run-lian LUO Xu-gang liao xiu-dong Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China Heilongjiang Provincial Animal Epidemic Prevention and Control Center Harbin 150069P.R.China Institute of Animal Husbandry Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China College of Animal Science and Technology Hebei Normal University of Science and TechnologyQinhuangdao 066004P.R.China Department of Animal Science Guangdong Ocean UniversityZhanjiang 524088P.R.China

Selenium(Se)is an essential trace element that has a large number of biological functions for ***,the absorption kinetics of Se from sodium selenite in the small intestine of broilers remains ***,two experiments were conducted with 28-d-old commercial male broilers to study the kinetics of Se absorption in ligated small intestinal *** experiment 1,the Se absorption in duodenal,jejunal,and ileal segments at different post-perfusion time points(0,20,40,60,80,100 and 120 min)were *** experiment 2,a kinetic study of Se absorption was conducted with the duodenal,jejunal,and ileal loops perfused with solutions containing 0,0.0375,0.075,0.15,0.30,or 0.60μg mL^-1 of Se as sodium selenite,and Se contents in perfusates were determined at 100 min after *** results from experiment 1 showed that the Se absorption increased in an asymptotic response(P0.05)in the Se absorption rate among different ligated small intestinal segments perfused with solutions containing 0.0375–0.15μg mL^-1 of Se,however,the Se absorption rate was higher(P<0.05)in the jejunum than that in the duodenum perfused with solutions containing 0.30–0.60μg mL^-1 of *** kinetic curves of Se absorption demonstrated that the Se absorption was a saturated carrier-mediated process in the duodenum,and the maximum absorption rate was 1271 pg min^-1 cm^-1;whereas the Se absorptions were a non-saturated diffusion process in the jejunum and ileum,and the diffusive constants were 2107 and 1777 cm2 min^-1,*** results from the present study indicate that the jejunum is the main Se absorption site,and the Se absorption i

关键词： broiler small intestine selenium absorption kinetics

Effect of dietary supplementation of pyrroloquinoline quinone disodium on growth performance, meat quality and antioxidative ability of broilers

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第7期19卷 1850-1856页

作者： LIU Guo-qing SUN Guang-ming liao xiu-dong HUANG Jian-zhong GUO Mei-jin ZHANG Li-yang GUO Yan-li LU Lin LUO Xu-gang Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural SciencesBeiing 100193P.R.China Department of Animal Science Gansu Agricultural UniversityLanzhou 730070P.R.China Fujian Ubaifu Biotechnology Co.Ltd. Fuzhou 350108P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China

This study was conducted to investigate the effect of dietary supplementation with pyrroloquinoline quinone(PQQ) in the form of PQQ disodium(PQQ·Na2) on the growth performance, carcass traits, meat quality and antioxidative ability of broilers. A total of 720 one-d-old Arbor Acres male broilers were randomly allocated to 1 of 6 treatments with 8 replicates of 15 birds per replicate in a completely randomized design. Birds were fed a PQQ·Na2-unsupplemented corn-soybean meal basal diet(control) or the basal diet supplemented with 0.1, 0.2, 0.3, 0.4 or 0.5 mg PQQ·Na2 kg-1 for 42 d. Compared with the control chicks, the chicks fed the diets supplemented with PQQ·Na2 had lower(P<0.05) feed:gain(F/G) during the grower phase and drip losses of breast muscles on day 42. As supplemental PQQ·Na2 level increased, plasma total antioxidant capacity(T-AOC) on d 42, liver T-AOC on d 21 and heart T-AOC on d 21 and 42 increased linearly(P<0.05), but malondialdehyde concentrations in plasma, liver and heart on d 21 or 42 decreased linearly(P<0.001) or quadratically(P<0.005). The results from the present study indicate that dietary supplemental PQQ·Na2 can improve antioxidant ability and meat quality of broilers, and in general, it is implied that the optimal supplemental PQQ·Na2 level is 0.1 mg kg-1 of diet for broilers from 1 to 42 d of age.

关键词： antioxidation broiler growth performance meat quality pyrroloquinoline quinone disodium