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Hypomethylation of glycine dehydrogenase promoter in peripheral blood mononuclear cells is a new diagnostic marker of hepatitis B virus-associated hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 35-42页

作者： li-li Miao Jing-wen Wang Hui-Hui liu Shuai Gao Yu-Chen Fan Kai Wang Department of Hepatology Qilu Hospital of Shandong UniversityJinan 250012China Experimental Center Shandong University of Traditional Chinese MedicineJinan 250355China Institute of Hepatology Shandong UniversityJinan 250012China

Background: Glycine dehydrogenase(GLDC) plays an important role in the initiation and proliferation of several human cancers. In this study, we aimed to detect the methylation status of GLDC promoter and its diagnostic value for hepatitis B virus-associated hepatocellular carcinoma(HBV-HCC). Methods: We enrolled 197 patients, 111 with HBV-HCC, 51 with chronic hepatitis B(CHB), and 35 healthy controls(HCs). The methylation status of GLDC promoter in peripheral mononuclear cells(PBMCs) was identified by methylation specific polymerase chain reaction(MSP). The mRNA expression was examined using real-time quantitative polymerase chain reaction(q PCR). Results: The methylation frequency of the GLDC promoter was significantly lower in HBV-HCC patients(27.0%) compared to that in CHB patients(68.6%) and HCs(74.3%)( P < 0.001). The methylated group had lower alanine aminotransferase level( P = 0.035) and lower rates of tumor node metastasis(TNM) Ⅲ/Ⅳ( P = 0.043) and T3/T4( P = 0.026). TNM stage was identified to be an independent factor for GLDC promoter methylation. GLDC mRNA levels in CHB patients and HCs were significantly lower than those in HBV-HCC patients( P = 0.022 and P < 0.001, respectively). GLDC mRNA levels were significantly higher in HBV-HCC patients with unmethylated GLDC promoters than those with methylated GLDC promoters( P = 0.003). The diagnostic accuracy of alpha-fetoprotein(AFP) combined with GLDC promoter methylation for HBV-HCC was improved compared with that of AFP alone(AUC: 0.782 vs. 0.630, P < 0.001). In addition, GLDC promoter methylation was an independent predictor for overall survival of HBV-HCC patients( P = 0.038). Conclusions: The methylation frequency of GLDC promoter was lower in PBMCs from HBV-HCC patients than that from patients with CHB and HCs. The combination of AFP and GLDC promoter hypomethylation significantly improved the diagnostic accuracy of HBV-HCC.

关键词： Hepatocellular carcinoma Glycine dehydrogenase DNA methylation Peripheral blood mononuclear cells

A Composite Elastomer with Photo-responsive Shape Memory and Programmable Hygroscopic Actuation Functionalities

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Chinese Journal of Polymer Science 2024年第10期42卷 1470-1478,I0009页

作者： Xiang-Xi Cui li Shang Zhong-wen liu Zhao-Tie liu Jin-Qiang Jiang Guo li Key Laboratory of Syngas Conversion of Shaanxi Province School of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’an710062China

Developing hydroscopic actuators with simultaneous high elasticity,shape programmability and tunable actuating behaviors are highly desired but still *** this study,we propose an orthogonal composite design to develop such a *** developed composite elastomer comprises carboxyl group-grafted polystyrene-block-poly(ethylene-co-butylene)-block-polystyrene(SEBS-g-COOH)as the elastic substrate,and a synthesized azobenzene derivative as the functional filler(Azo12).By surface treatment using acidic and base solutions,the carboxyl groups on the surface can reversibly transform into carboxylate groups,which render the composite tunable hygroscopic actuating *** another aspect,the added filler undergoes trans-to-cis isomerization when exposed to UV light irradiation,leading to liquefaction of the crystalline aggregates formed by Azo12 *** liquefied Azo12 molecules can autonomously resotre their trans form and reform the crystalline *** reversible change in crystralline structure is utilized to realize the shape memory property,and 5 wt%of Azo12addition is adequate for the composite to exhibit photo-responsive shape memory behavior without compromising much of the *** regualtion of external geometry by shape memory effect is effective in altering the actuating *** proposed method can be extend to designing different composites with the demonstrated functionalities.

关键词： Actuator Shape memory Photo-responsive Hygroscopic responsive Azobenzene

Palmitic acid reduces the methylation of the FOXO1 promoter to suppress the development of diffuse large B-cell lymphoma via modulating the miR-429/DNMT3A axis

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Chinese Journal of Natural Medicines 2024年第6期22卷 554-567页

作者： li Weiming GAO Ming XUE Weili li Xiaoli CHANG Yu ZHANG Kaixin wen Chenyu ZHANG Mingzhi Department of Oncology Henan University of Traditional Chinese MedicineZhengzhou 450000China Department of Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China

Diffuse large B-cell lymphoma(DLBCL)is characterized by significant treatment *** acid(PA)has shown promising antitumor *** study aims to elucidate the molecular mechanisms by which PA influences DLBCL *** quantified the expression levels of microRNAs(miRNAs),Forkhead box protein O1(FOXO1),and DNA methyltransferase 3A(DNMT3A)in both untreated and PA-treated DLBCL tumors and cell *** were made of cell viability,apoptosis,and autophagy-related protein expression following PA *** analyses among miR-429,DNMT3A,and FOXO1 were conducted using luciferase reporter assays and methylation-specific(MSP)Polymerase chain reaction(PCR).After transfecting the miR-429 inhibitor,negative control(NC)inhibitor,shRNA against DNMT3A(sh-DNMT3A),shRNA negative control(sh-NC),over-expression vector for DNMT3A(oe-DNMT3A),or overexpression negative control(oe-NC),we evaluated the effects of miR-429 and DNMT3A on cell viability,mortality,and autophagy-related protein expression in PA-treated DLBCL cell *** efficacy of PA was also tested in vivo using DLBCL tumor-bearing mouse ***-429 and FOXO1 expression levels were downregulated,whereas DNMT3A was upregulated in DLBCL compared to the control *** treatment was associated with enhanced autophagy,mediated by the upregulation of miR-429 and downregulation of *** luciferase reporter assay and MSP confirmed that miR-429 directly inhibits DNMT3A,thereby reducing FOXO1 *** experiments demonstrated that PA promotes autophagy and inhibits DLBCL progression by upregulating miR-429 and modulating the DNMT3A/FOXO1 *** vivo PA signific-antly reduced the growth of xenografted tumors through its regulatory impact on the miR-429/DNMT3A/FOXO1 *** acid may modulate autophagy and inhibit DLBCL progression by targeting the miR-429/DNMT3A/FOXO1 signaling pathway,suggesting a novel therapeutic target for DLBCL management.

关键词： Palmitic acid Diffuse large B-cell lymphoma MiR-429 DNMT3A FOXO1 Autophagy

Optimal Design and Experimental Study of Tightly Coupled SCR Mixers for Diesel Engines

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Energy Engineering 2024年第10期121卷 2893-2906页

作者： Jianhua Zhang wen Sun Zhijun li State Key Laboratory of Engines Tianjin UnivesityTianjin300072China Weichai Power Emission Solutions Technology Co. Ltd.Weifang261061China School of Energy and Power Engineering Shandong UniversityJinan250061China

Two types of tightly coupled Selective Catalytic Reduction(SCR)mixers were designed in this study,namely Mixer 1 integrated with an SCR catalyst and Mixer 2 arranged *** Fluid Dynamics(CFD)software was utilized to model the gas flow,spraying,and pyrolysis reaction of the aqueous urea solution in the tightly coupled SCR *** parameters of gas flow velocity uniformity and ammonia distribution uniformity were simulated and calculated for both Mixer 1 and Mixer 2 in the tightly coupled SCR system to compare their advantages and *** simulation results indicated that Mixer 1 exhibited a gas velocity uniformity of 0.972 and an ammonia distribution uniformity of 0.817,whereas Mixer 2 demonstrated a gas velocity uniformity of 0.988 and an ammonia distribution uniformity of *** 2 performed better in the simulation ***,a 3D-printed prototype of Mixer 2 was manufactured and installed on an engine test bench to investigate ammonia distribution uniformity and NOX conversion *** experimental investigations yielded the following findings:1)The ammonia distribution uniformity of Mixer 2 was measured as 0.976,which closely aligned with the simulation result of 0.964,with a deviation of 1.2%from the model calculations;2)As exhaust temperature increased,the ammonia distribution uniformity gradually improved,while an increase in exhaust flow rate resulted in a decrease in ammonia distribution uniformity;3)When utilizing Mixer 2,the NOX conversion efficiency reached 84.7%at an exhaust temperature of 200°C and 97.4%at 250°*** the exhaust temperature range of 300°C to 450°C,the NOX conversion efficiency remained above 98%.This study proposed two innovative mixer structures,conducted simulation analysis,and performed performance *** research outcomes indicated that the separately arranged Mixer 2 exhibited superior *** tightly coupled SCR systemequippedwith Mixer 2 achieved excellent levels

关键词： Tightly coupled SCR mixer ammonia distribution uniformity

Epigenetics:Mechanisms,potential roles,and therapeutic strategies in cancer progression

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Genes & Diseases 2024年第5期11卷 300-314页

作者： Dong Wang Yan Zhang Qingbo li Yu li wen li Ao Zhang Jingxuan Xu Jingyan Meng lin Tang Shuhua Lyu Graduate School Tianjin University of Traditional Chinese MedicineTianjin 301617China College of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Pathology Tianjin Union Medical CenterTianjin 300121China

Mutations or abnormal expression of oncogenes and tumor suppressor genes are known to cause *** studies have shown that epigenetic modifications are key drivers of cancer development and ***,the mechanistic role of epigenetic dysregulation in the tumor microenvironment is not fully ***,we reviewed the role of epigenetic modifications of cancer cells and non-cancer cells in the tumor microenvironment and recent research advances in cancer epigenetic *** addition,we discussed the great potential of epigenetic combination therapies in the clinical treatment of ***,there are still some challenges in the field of cancer epigenetics,such as epigenetic tumor heterogeneity,epigenetic drug heterogeneity,and crosstalk between epigenetics,proteomics,metabolomics,and other omics,which may be the focus and difficulty of cancer treatment in the *** conclusion,epigenetic modifications in the tumor microenvironment are essential for future epigenetic drug development and the comprehensive treatment of *** combination therapy may be a novel strategy for the future clinical treatment of cancer.

关键词： Cancer Epigenetics Gene expression Heterogeneity Tumor microenvironment

Azacitidine maintenance therapy for blastic plasmacytoid dendritic cell neoplasm allograft: A case report

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World Journal of Clinical Cases 2024年第1期12卷 136-141页

作者： li-li Tao Hui-Ting wen Zi-Yi Wang Juan Cheng li Zhao Department of Hematology The First Clinical Medical CollegeLanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of Hematology The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina

BACKGROUND Blastic plasmacytoid dendritic cell neoplasm(BPDCN)is a rare,highly invasive malignant *** is no universally accepted standard of care because of its rarity and the dearth of prospective *** is still challenging for some patients to achieve persistent clinical remission or cure,despite the success of allogeneic hematopoietic stem cell transplantation(allo-HSCT),indicating that there is still a significant recurrence *** report a case of prevention of BPDCN allograft recurrence by azacitidine maintenance therapy and review the relevant *** SUMMARY We report a 41-year-old man with BPDCN who was admitted to hospital due to skin sclerosis for>5 mo’*** was diagnosed by combined clinical assessment and laboratory *** diagnosis,the patients underwent induction consolidation chemotherapy to achieve the first complete remission,followed by bridging ***-transplantation,azacitidine(75 mg/m2 for 7 d)was administered as maintenance therapy,with repeat administration every 4–6 wk and appropriate extension of the chemotherapy *** 10 cycles,the patient has been disease free for 26 mo after *** assessments of bone marrow morphology,minimal residual disease,full donor chimerism,Epstein–Barr virus,and cytomegalovirus all yielded normal results with no abnormalities *** Azacitidine may be a safe and effective maintenance treatment for BPDCN following transplantation because there were no overt adverse events during the course of treatment.

关键词： Blastic plasmacytoid dendritic cell neoplasm Azacitidine Allogeneic hematopoietic stem cell transplantation Maintenance therapy Case report

Permo-Carboniferous Sequence Stratigraphy and Sea Level Changes in North China Platform

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Journal of Earth Science 1999年第2期18卷 47-55页

作者： li Baofang li Guidong wen Xianduan Department of Energy Geology, China University of Geosciences, Beijing 100083

Through a sequence stratigraphic research on the Permo-Carboniferous in North China, it is suggested that the boundary of the Carboniferous and the Permian may be better moved down to the bottom of the main workable coalbed which is overlain by the limestones containing Pseudoschwagerina zone. This study mainly deals with the Upper Carboniferous, the Lower Permian and the lower part of the Middle Permian coal bearing strata, which are 150-180 m thick totally and can be divided into 2 mesosequences, including 6 sequence sets and 19 sequences. The lithostratigraphic units are diachronous in North China. The upper two limestones of the Taiyuan Formation in the central part of the study area may be correlated with the bottom two limestones of the lower Taiyuan Formation in the southern part. The Shanxi Formation in the central and northern parts may be correlated with most of the Taiyuan Formation in the southern part. The Xiashihezi Formation in the northern part may be correlated with the upper part of Shanxi Formation in the central and southern parts. The Shangshihezi Formation in the northern part may be correlated with the Xiashihezi Formation in the southern part. The paleogeographical maps are compiled in a chrono stratigraphic framework. From the Late Carboniferous to the Early Permian, 3 cycles of sea level change and transgression regression occurred, and the major transgression took place in the Asselian. In the Late Carboniferous, the direction of transgression was from the east to the west, and in the Early Permian it was from the southeast and the southwest to the north. Three workable coalbed groups were formed in Asselian, early Sakmarian and late Sakmarian respectively. The TST coals developed under the bottom of the Asselian limestones are the best coals for coalbed meathane extracting. The Sakmarian coal beds were formed in HST. The coal beds of Lower Permian distribute southward along with the relative sea level falls and sediment prograd

关键词： sequence stratigraphy sea level change lithofacies and paleogeography Permo-Carboniferous North China.

Enhancement of beneficial microbiomes in plant-soil continuums through organic fertilization:Insights into the composition and multifunctionality

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Soil Ecology Letters 2024年第3期6卷 109-120页

作者： wen-Jing li Xin-Yuan Zhou Xin-li An li-Juan li Chen-Shuo lin Hua li Hong-Zhe li Key Lab of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of EnvironmentResourceSoil and FertilizersZhejiang Academy of Agricultural SciencesHangzhou 310021China

Soil microbiomes play a crucial role in maintaining ecological functions and are of great importance for soil *** of them could bring benefits to plants for growth *** numerous studies have focused on specific beneficial bacteria and their interactions with soils and plants,we still lack a comprehensive understanding of beneficial communities in plant–soil continuums and their responses to agricultural *** address this gap,we carried out a microcosm experiment using 16S rRNA amplicon sequencing to explore the effects of organic fertilization on beneficial communities in plant–soil continuums and assess their potential *** findings reveal that organic fertilization had a positive impact on the beneficial functionality of bacterial communities in plant–soil *** improvement was primarily attributed to the optimized soil physicochemical conditions resulting from organic ***,organic fertilization increased the complexity of bacterial co-occurrence networks in both soils and the *** taxa in the endosphere undergone a shift of functions toward pathogen suppression as the result of organic ***,this study revealed that plants exhibited a preference for internalizing beneficial bacteria over other type of *** also provided new insights for evaluating the multifunctionality of microbiomes,and found that the functionality of beneficial communities in plant–soil continuums is enhanced by organic *** these findings suggested that organic fertilization can be an effective strategy for maintaining plant and soil health.

关键词： plant–soil continuums beneficial bacteria agricultural activities multifunctionality endosphere

Numerical Study on Local Flow and Heat Transfer Characteristics of Supercritical CO_(2) in PCHE with Sinusoidal Channels

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Journal of Thermal Science 2024年第1期33卷 41-55页

作者： wenZhexi LV Yigao li Qing WU Jinglin School of Energy Science and Engineering Central South UniversityChangsha 410083China

Local heat transfer and flow characteristics,which is crucial to the overall performance of supercritical CO_(2) recuperators,is rarely examined in details in reported *** this paper,the local heat transfer and flow characteristics of supercritical CO_(2) in sinusoidal channel printed circuit heat exchangers are numerically investigated under the working conditions of *** on the simplified physical model constituted by10 pitches,the variations of Re,heat flux,Nu,secondary flow and other relevant parameters along the flow direction are analyzed *** is further made between the high and low temperature recuperators(HTR and LTR).It's observed that the local heat transfer and flow characteristics vary greatly from the inlet to the outlet,especially in the *** of 127.5% and 61.7% can be observed on the cold side of the LTR for Re and heat transfer coefficient h, *** indicate that the temperature difference and heat transfer coefficient h should not be regarded as constant and the distribution of h should be carefully considered in the design of the *** complicated interactions among the varying thermophysical properties,buoyancy and the periodically changed centrifugal force are believed to be the key that shapes the flow ***,the changes of the wavy angle θ are found to have greater influence than the changes of mass flow rate in reshaping the flow field when θ>25°.Though gravity direction strongly affects the local heat transfer and flow characteristics,it's also found that the effects on the overall thermal-hydraulic performance are relatively *** the installation direction that yields a_y=g should better be avoided for the sinusoidal channel printed circuit heat exchanger.

关键词： printed circuit heat exchanger supercritical CO_(2) sinusoidal channel heat transfer recuperator