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信阳市自然保护区现状调查与规划研究

信阳农业高等专科学校学报 2000年第2期10卷 23-26页

作者：李新李杰王琴信阳市环境科研所信阳市环保局信阳农业高等专科学校信阳464000

在对信阳市自然保护区现状调查的基础上 ,根据信阳市自然环境实际 ,参照国家及河南省自然保护区建设与规划要求 ,对信阳市自然保护区进行了规划研究。

关键词：信阳市现状自然保护区生态环境发展规划管理

高粱EST-SSR标记的建立及其在苏丹草中的应用初探

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草业科学 2010年第3期27卷 112-117页

作者：李杰勤王丽华詹秋文陈亚安徽科技学院安徽凤阳233100

从NCBI中下载了202 325条高粱Sorghumbicolor EST序列。去除低质量和冗余的序列后,在5 661条高粱EST中共发掘出了6 663个SSR位点,出现频率是20.19%,平均分布频率是1/3.93 kb。在5 197条EST序列中,共有3 446条序列能够设计引物,占总数的6... 详细信息

从NCBI中下载了202 325条高粱Sorghumbicolor EST序列。去除低质量和冗余的序列后,在5 661条高粱EST中共发掘出了6 663个SSR位点,出现频率是20.19%,平均分布频率是1/3.93 kb。在5 197条EST序列中,共有3 446条序列能够设计引物,占总数的66.3%。在高粱EST-SSR中,三核苷酸重复是主要的重复类型,出现最多的重复基元是GGC/GCC。在3 446对高粱EST-SSR引物中,随机选择了20对引物进行了合成。以苏丹草Sorghumsudanense722和高粱Sorghumbicolor TX623A为模板,用这20对引物进行PCR扩增,结果全部可以扩增出条带,可用率为100%,有差异的有4对,多态性比率为20%。研究结果证明了根据高粱EST建立SSR标记是有效、可行的。

关键词：高粱苏丹草 EST-SSR

用(培矮64s/Nipponbare)F2群体对水稻产量构成性状的QTL定位分析

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作物学报 2005年第12期31卷 1620-1627页

作者：叶少平张启军李杰勤赵兵李平四川农业大学水稻研究所

用水稻测序品种培矮64s和Nipponbare为亲本构建的含137个SSRs标记的连锁遗传图谱和(培矮64s/Nipponbare)F2群体的180个单株,对水稻的单株有效穗数、穗粒数、穗实粒数、结实率、穗着粒密度、千粒重等6个产量构成性状进行了QTL定位分析。... 详细信息

用水稻测序品种培矮64s和Nipponbare为亲本构建的含137个SSRs标记的连锁遗传图谱和(培矮64s/Nipponbare)F2群体的180个单株,对水稻的单株有效穗数、穗粒数、穗实粒数、结实率、穗着粒密度、千粒重等6个产量构成性状进行了QTL定位分析。共检测到6个性状的22个QTLs,分布在第1、2、4、5、6、9、10、11、12等9条染色体的14个区域,表型贡献率5.0%～19.3%;相关性较强的性状之间具有较多共同或紧密连锁的QTLs;集中分布的QTLs之间既有同向连锁,也有反向连锁。对不同水稻群体定位的同源QTL进行了比较,对QTL在染色体上的集中分布,以及用QTL定位结果和生物信息学方法相结合预测基因的功能等进行了探讨。

关键词：水稻测序品种 SSR 产量构成性状 QTL定位生物信息学

Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan qin lin Zhu Teng Jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

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马杜拉分支菌引起下颌部真菌性足菌肿一例

中华皮肤科杂志 2007年第8期40卷 461-463页

作者：闫洁邓军郝飞钟白玉李芹阶周村建第三军医大学西南医院皮肤科重庆400038

目的探讨1例真菌性足菌肿的临床病理特点、诊断、治疗及预后,并对其致病菌进行鉴定。方法分析我科收治的1例下颌部真菌性足菌肿的临床及病理特点,进行真菌学及分子生物学鉴定。结果该患者以下颌部肿胀、多发窦道形成、排大量黑色颗粒为... 详细信息

目的探讨1例真菌性足菌肿的临床病理特点、诊断、治疗及预后,并对其致病菌进行鉴定。方法分析我科收治的1例下颌部真菌性足菌肿的临床及病理特点,进行真菌学及分子生物学鉴定。结果该患者以下颌部肿胀、多发窦道形成、排大量黑色颗粒为主要症状,病理检查示化脓性肉芽肿性炎症,PAS染色可见大量藕节状真菌菌丝。ITS1、ITS2和D1/D2区测序结果示:病原菌与足菌肿马杜拉分支菌有最大的相似性,但相似性小于97%。结论本病例为发生在下颌部的由马杜拉分支菌属内一新的菌种引起的真菌性足菌肿。

关键词：马杜拉分枝菌属马杜拉分枝菌病真菌性足菌肿

高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位

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草业学报 2010年第5期19卷 273-277页

作者：李杰勤王丽华詹秋文范军成安徽科技学院植物科学学院安徽凤阳233100

高粱棕色突变体的叶片中脉是棕色的,此类突变体能使家畜难以消化的木质素含量降低40%～60%,从而大幅度提高了家畜对高粱秸秆的消化率。利用高粱棕色中脉材料N592(bmr-6类型)和白色中脉(Sa)进行杂交和自交,构建F2分离群体,F1表现为白色中... 详细信息

高粱棕色突变体的叶片中脉是棕色的,此类突变体能使家畜难以消化的木质素含量降低40%～60%,从而大幅度提高了家畜对高粱秸秆的消化率。利用高粱棕色中脉材料N592(bmr-6类型)和白色中脉(Sa)进行杂交和自交,构建F2分离群体,F1表现为白色中脉,F2白棕分离比符合3∶1,说明棕色中脉基因受一对等位基因控制,表现为隐性遗传。用已知定位到连锁群上的微卫星标记对棕色中脉基因进行了连锁分析,发现1个与棕色中脉(bmr-6)基因连锁的微卫星标记,连锁距离为4.2cM,初步将该基因定位于第7连锁群。

关键词：高粱 bmr-6 SSR 基因定位

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甄氏外瓶霉致暗色丝孢霉病

临床皮肤科杂志 2009年第3期38卷 161-163页

作者：周村建邓军王莉钟白玉李芹阶郝飞第三军医大学西南医院皮肤性病科重庆400038

报告1例甄氏外瓶霉感染所致的暗色丝孢霉病。患者男,59岁。右小腿外踝上方肿块伴瘙痒5个月,皮损为一直径约3 cm的暗红色斑块。皮损组织病理PAS染色见分隔菌丝,组织块马铃薯葡萄糖琼脂(PDA)培养基上25℃培养长出黑色酵母样菌落,分离菌株... 详细信息

报告1例甄氏外瓶霉感染所致的暗色丝孢霉病。患者男,59岁。右小腿外踝上方肿块伴瘙痒5个月,皮损为一直径约3 cm的暗红色斑块。皮损组织病理PAS染色见分隔菌丝,组织块马铃薯葡萄糖琼脂(PDA)培养基上25℃培养长出黑色酵母样菌落,分离菌株最高生长温度37℃,小培养下见分枝分隔菌丝,环痕产孢,分生孢子聚集在环痕梗顶端,分离菌株ITS1区测序结果与甄氏外瓶霉98.54%同源。根据分离菌株生物学特点,菌株被确定为甄氏外瓶霉。

关键词：甄氏外瓶霉暗色丝孢霉病

Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： Yiyang qin Wenzhen Zhu Tingting Guo Yiran Zhang Tingting Xing Peng Yin Shihua li Xiao-Jiang li Su Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

Magnetron sputtered nickel oxide with suppressed interfacial defect states for efficient inverted perovskite solar cells

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Journal of Energy Chemistry 2025年第1期100卷 348-355页

作者： Guoqiang Ma qin Tan Zhaoning li Jingwei Xiu Jiafeng Wang Tianle Cheng Dong He Qiang Sun Xuhang Ma Francesco Lamberti Zhubing He Department of Materials Science and Engineering Institute of Innovative Materials (I2M) Shenzhen Key Laboratory of Full Spectral Solar Electricity Generation (FSSEG)Southern University of Science and Technology (SUSTech) Core Research Facilities Southern University of Science and Technology Department of Chemical Sciences Department of Industrial Engineering University of Padova

Widely used spin-coated nickle oxide （NiOx） based perovskite solar cells often suffer from severe interfacial reactions between the NiOxand adjacent perovskite layers due to surface defect states,which inherently impair device performance in a long-term view,even with surface molecule *** this study,we developed high-quality magnetron-sputtered NiOxthin films through detailed process optimization,and compared systematically sputtered and spin-coated NiOxthin film surfaces from materials to *** sputtered NiOxfilms exhibit improved crystallinity,smoother surfaces,and significantly reduced Ni3+or Ni vacancies compared to their spin-coated ***,the interface between the perovskite and sputtered NiOxfilm shows a substantially reduced density of defect *** solar cells （PSCs） fabricated with our optimally sputtered NiOxfilms achieved a high power conversion efficiency （PCE） of up to 19.93%and demonstrated enhanced stability,maintaining 86.2% efficiency during 500 h of maximum power point tracking under one standard sun ***,with the surface modification using (4-(2,7-dibromo-9,9-dimethylacridin-10（9H）-yl)butyl)p hosphonic acid （DMAcPA）,the device PCE was further promoted to 23.07%,which is the highest value reported for sputtered NiOxbased PSCs so far.

关键词： Nickle oxide Magnetron sputter Defect Stability Perovskite solar cell