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Journal of Iron and Steel Research International 2024年第7期31卷 1746-1762页

作者： Wei liu hong-li zhao Bing-xing Wang Yong Tian State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819LiaoningChina

The high-strength low-alloy steel plates with varying Ni/Mo contents were manufactured using the thermos-mechanical control *** investigation was conducted to explore the effect of Ni/Mo microalloying on microstructure evolution and mechanical properties of the *** results revealed that the increase in Ni content from 1 to 2 wt.%reduced the transition temperature of ferrite and the growth range of ferritic grain was narrowed,which promoted grain *** optimized combination of grain size,high-angle grain boundaries(HAGBs),and martensite-austenite(M-A)islands parameter contributed to the excellent impact toughness of S1 steel at-100℃(impact absorbed energy of 218.2 J at-100℃).As the Mo increases from 0 to 2 wt.%,the matrix structure changes from multiphase structure to granular bainite,which increases the average effective grain size to~4.62 pm and reduces HAGBs proportion to~36.22%.With these changes,the low-temperature impact toughness of S3 steel is *** addition,based on the analysis of the characteristics of crack propagation path,it was found that M-A islands with low content(~2.21%)and small size(~1.76 pm)significantly retarded crack propagation,and the fracture model of M-A islands with different morphologies was further ***,correlation between behaviour of delamination and toughness was further analysed by observing delamination size and impact energy parameters.

关键词： High-strength low-alloy steel Microstructural regulation Ni addition Mo addition Crack propagation Low-temperature toughness

K-19 mRNA RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer

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World Journal of Gastroenterology 2006年第32期12卷 5219-5222页

作者： Jian Suo Quan Wang hong-Juan Jin hong li Hang zhao Department of General Surgery First Hospital Changchun 130021 Jilin Province China

AIM： To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS： Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of gastric carcinoma. RESULTS： K19 mRNA was expressed in all tumor specimens of 30 cases; of the 126 lymph nodes, 26 were histopathologically positive （20.6%）, and 42 positive （33.3%） by RT-PCR. Amplification fragments of 460 and 540 bp were shown in all the tumor tissues and metastatic lymph nodes after K19 and β-actin RT-PCR, while only a 540 bp fragment appeared in the lymph nodes of non-tumor patients. CONCLUSION： K19 mRNA RT-PCR is sensitive and specific in testing micrometastasis in regional lymph nodes of gastric cancer, and it is superior to routine histopathology.

关键词： K-19 mRNA RT-PCR Micrometastasis Gastric cancer

Novel modified pectin for heavy metal adsorption

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Chinese Chemical Letters 2007年第3期18卷 325-328页

作者： Feng Ting li hong Yang Yan zhao Ran Xu State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and Engineering Tongji University Shanghai 200092 China

Modified pectin cross-linked with adipic acid, was synthesized and used for heavy metal removal from wastewater. SEM and FTIR were used to investigate its structure and morphology. The modified pectin had a rough, porous phase covered with carboxy groups, resulting a high adsorption capacity. And at the room temperature, the saturated loading capacity for Pb^2＋, Cu^2＋ and Zn^2＋ reached 1.82 retool/g, 1.794 mmol/g and 0.964 retool/g, respectively. The results proved its potential application to remove of the heavy metal.

关键词： Modified pectin Adsorption Heavy metal

Experimental study on a -60 °C cascade refrigerator with dual running mode

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第5期13卷 375-381页

作者： Xiu-fang liU Jiong-hui liU hong-li zhao Qiao-yu ZHANG Juan-li MA Institute of Refrigeration and Cryogenic Engineering Xi'an Jiaotong University Xi 'an 710049 China School of Mechanical and Power Engineering Henan Polytechnic University Jiaozuo 454000 China

In this study,an experimental setup is designed and built to investigate the feasibility and performance of the proposed dual-mode cascade refrigeration *** apparatus can be operated in two modes:dual-stage mode and single-stage mode such that the low temperature cycle(LTC) can be operated together with the high temperature cycle(HTC) or can run *** results validate the feasibility of independent operation of *** performance of the independent operation of LTC mode is theoretically investigated using experimental data as *** suggestions are made for the improvement of the coefficient of performance(COP) of the experimental ***,high COP of the cycle provides excellent application for the presented refrigeration cycle.

关键词： Cascade refrigeration Dual-mode Regenerator Optimization Effectiveness

Expression of inducible nitric oxide synthase in the brain tissue of rats at preoxygenation, hypoxia/reoxygenation

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Neural Regeneration Research 2006年第8期1卷 689-692页

作者： hongzhi Chen Lu li hong zhao Department of Anesthesiology Shengjing Hospital China Medical University Shenyang 110004 Liaoning ProvinceChina Department of AnesthesiologyShengjing Hospital China Medical University Shenyang 110004 Liaoning ProvinceChina Department of AnesthesiologyShengjing Hospital China Medical University Shenyang 110004 Liaoning ProvinceChina

BACKGROUND: The disorder of the respiratory and circulation system during the anesthesia and operation often can lead to severe hypoxia. Preoxygenation is a conventional therapy for treatment of hypoxia. OBJECTIVE: To observe the change in the expression of inducible nitric oxide synthase(iNOS), i.e. brain tissue injury degree, in cerebral cortex of rats after hypoxia/reoxygenation under the condition of different levels of preoxygenation. DESIGN: Randomized controlled animal experiment. SETTING: Department of Anesthesiology and Central Laboratory, Shengjing Hospital Affiliated to China Medical University. MATERIALS: This experiment was carried out in the laboratory of Shengjing Hospital Affiliated to China Medical University from March 2003 to March 2004. Seventy-two male Wistar rats, weighing from 250 to 300 g, were provided by the Animal Experimental Room of Shengjing Hospital of China Medical University [license No. SYXK (liao) 2003-0019]. Seventy-two rats were randomized into 3 groups: preoxygenation group, hypoxia group and reoxygentation group. Each group was divided into 4 subgroups, 6 rats in each subgroup. METHODS: 0.21, 0.50, 0.75 and 0.98 volume fraction of oxygen was given to 4 subgroups of preoxygenation group respectively. The rats in each subgroup of hypoxia group inhaled oxygen for 30 minutes according to the method of preoxygenation. Then, nitrogen gas replaced oxygen and was pumped into the cabin, and the volume fraction of oxygen was decreased to be 0.05 within 20 minutes. The rats in each subgroup of reoxygenation group were treated with the experimental methods of preoxygenation and hypoxia separately, then they were moved into the wide mouthed bottle with two-air-duct rubber stopper. Finally, oxygen was pumped and the volume fraction of oxygen reached over 0.98 within 20 minutes. After 24 hours, all the surviving rats were killed by decapitation. Cerebral tissue was sliced and stained by haematoxylin-eosin and then enveloped. Brain tissue inju

关键词： volume fraction tissue injury nitric oxide synthase animal experiment result analysis neuronal injury cerebral cortex brain injury Wistar rats methods of method of

Preparation of 2,6-diisopropylnaphthalene with recycled process of isomers

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Chinese Chemical Letters 2008年第4期19卷 390-394页

作者： Zheng Hua Tian Gui li zhao hong Min Jia Ben li Wei Qi Hu School of Chemical Engineering University of Science ＆ Technology Liaoning.Anshan 114051China

2,6-Diisopropylnaphthalene（2,6-DIPN）,as the precursor of important monomer 2,6-naphthalene dicarboxylic acid,was prepared by hydroisopropylation of refined naphthalene with propene over shape-selective *** conversion of 92% and 2,6-DIPN selectivity of 64% were *** melt crystallization was applied to separate and purify 2,6-DIPN from its isomers,resulted in a product purity of≥99%.The other isomers were converted into monoisopropylnaphthalene,which also reacted with propene to form 2,6-DIPN.A recycled process including hydroisopropylation,separation and transalkylation was established,the yield of 2,6-DIPN based on naphthalene could be doubled by one cycle operation.

关键词： 2,6-Diisopropylnaphthalene Hydroisopropylation Static melt crystallization Intermolecular transalkylation Recycled process

Losartan reduced connexin43 expression in left ventricular myocardium of spontaneously hypertensive rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第6期9卷 448-454页

作者： li-li zhao hong-juan CHEN Jun-zhu CHEN Min YU Yun-lan NI Wei-fang ZHANG Department of Cardiology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective:To assess the effect of angiotensin II type 1(AT1)receptor antagonist losartan on myocardium con- nexin43(Cx43)gap junction(GJ)expression in spontaneously hypertensive rats(SHRs)and investigate possible mechanisms. Methods:Sixteen 9-week-old male SHRs and 8 age-matched male Wistar-Kyoto(WKY)rats were included in this *** were randomly divided into two groups to receive losartan at 30 mg/(kg·d)by oral gavage once daily for 8 weeks(SHR-L)or vehicle(0.9%saline)to act as controls(SHR-V);WKY rats receiving vehicle for 8 weeks served as normotensive *** the end of the experiment,rats were sacrificed and the hearts were *** of Cx43 and nuclear factor-kappaB p65 (NF-κB p65)proteins in all three groups were observed and further investigations on the effect of angiotensin II type 1 receptor antagonist losartan(30 mg/(kg·d),8 weeks)on Cx43 expression were conducted with Western blot and immunohistochemistry. NF-κB p65 protein in nuclear extracts was determined by Western ***:Left ventricular(LV)hypertrophy was prominent in SHRs,Cx43 and NF-κB p65 protein expressions were obviously upregulated and Cx43 distribution was dispersed over the cell *** with losarton reduced the over-expressions of Cx43 and NF-κB p65 in LV *** distribution of Cx43 gap junction also became much regular and confined to intercalated disk after losartan ***- sion:Cx43 level was upregulated in LV myocardium of SHR during early stage of *** II type 1 receptor antagonist losartan prevented Cx43 gap junction remodeling in hypertrophied left ventricles,possibly through the NF-κB pathway.

关键词： Connexin43 (Cx43) Left ventricular (LV) hypertrophy Angiotensin II Nuclear factor-kappaB p65 (NF-κB p65) Gap junction (GJ)

Dietary supplementation with acidifiers improves the growth performance, meat quality and intestinal health of broiler chickens

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Animal Nutrition 2021年第3期7卷 762-769页

作者： Chun-Qi Gao Hui-Qin Shi Wen-Yan Xie li-hong zhao Jian-Yun Zhang Cheng Ji Qiu-Gang Ma State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China College of Animal Science South China Agricultural UniversityGuangzhou510642China

This experiment was conducted to study the effects of dietary supplementation with acidifiers on the growth performance,meat quality,and intestinal health of broiler chickens.A total of 648 male Arbor Acres broiler chickens at 1 d old were randomly divided into 6 groups,and each group consisted of 6 replicates with 18 broilers per *** dietary treatments were as follows:negative control(NC,the basal diet),NC+antibiotic(enramycin,8 mg/kg,positive control[PC]),NC+phosphoric acid(PA,0.1,0.2,and 0.3 g/kg),and NC+lactic acid(LA,0.3 g/kg).The feeding trial lasted for 42 *** results showed that the feed-to-gain ratio of the NC+acidifier groups was lower than that of the NC and PC groups from 1 to 42 d(Plipase activities of the duodenum in the NC+PA(0.2 and 0.3 g/kg)groups,as well as the villus height-to-crypt depth(VH:CD)ratio of the duodenum in the NC+PA(0.1 g/kg)group was significantly greater(Pli and Salmonella in the cecum of the NC+PA(0.1 g/kg)and LA(0.3 g/kg)groups were decreased(Plity,and intestinal health of broilers,in which the effects of PA(0.1 g/kg and 0.2 g/kg)are better than the other supplementations.

关键词： Antibiotic Acidifier Growth performance Meat quality Intestinal health Broiler

A Comparison of the Biological Characteristics of EV71 C4 Subtypes from Different Epidemic Strains

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Virologica Sinica 2010年第2期25卷 98-106页

作者： li-chun WANG Song-qing TANG Yan-mei li hong-lin zhao Cheng-hong DONG Ping-fang CUI Shao-hui MA Yun liAO Long-ding liU Qi-han li Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College Kunming 650118 China

The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.

关键词： Enterovirus 71 (EV71) Subtype C4 Epidemic strain Hand-foot and mouth disease (HFMD)

Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 731-736页

作者： li-hong zhao~1* Yu-Yan MA~2 hong WANG~1 Shu-Ping zhao~3 Wei-Ming zhao~1 Hua li~2 Lei-Yi WANG~2 1 Department of Microbiology,School of Medicine,Shandong University,Jinan 250012,China 2 Qilu Hospital,Shandong University,Jinan 250012,China 3 Tai'an central Hospital,Tai'an 271000,China 4 Department of Virology,School of Public Health,Shandong University,Jinan 250012,China Department of Microbiology School of Medicine Shandong UniversityJinan 250012 China Qilu Hospital Shandong UniversityJinan 250012 China Tai'an central Hospital Tai'an 271000 China

The aim of this study was to establish and apply a real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） for rubella virus （RV） ***,the primer and TaqMan probeconcentrations,as well as reaction temperatures were optimized to establish an efficient real-time quantitativeRT-PCR assay for RV ***,an RV-specific PCR amplicon was made as an external standard toestimate the linearity,amplification efficiency,analytical sensitivity and reproducibility of the real timequantitative ***,the assay was applied to quantify RV RNA in clinical samples for rubella *** RV-specific PCR amplicon was prepared for evaluation of the assay at 503 bp,and its original concentrationwas 2.75×10～9 copies/μ*** real time quantitative assay was shown to have good linearity （R2=0.9920）,highamplification efficiency （E=1.91）,high sensitivity （275 copies/ml）,and high reproducibility （variation coefficientrange,from 1.25% to 3.58%）.Compared with the gold standard,the specificity and sensitivity of the assayin clinical samples was 96.4% and 86.4%,***,the established quantitative RT-PCRmethod is a simple,rapid,less-labored,quantitative,highly specific and sensitive assay for RV RNA.

关键词： rubella virus PCR amplicon real-time fluorescence quantitative RT-PCR