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TOPK Inhibition Enhances the Sensitivity of Colorectal Cancer Cells to Radiotherapy by Reducing the DNA Damage Response

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Current Medical Science 2024年第3期44卷 545-553页

作者： Shi-gui PANG Xin ZHANG Zhao-xin li li-fei he Feng CheN Ming-long liU Ying-ze HUANG Jian-mei MO Kong-lan LUO Juan-juan XIAO Feng ZHU Department of Oncology The Affiliated Hospital of Guilin Medical UniversityGuilin541001China Cancer Research Institute The Affiliated Hospital of Guilin Medical UniversityGuilin541001China Translational Medical Center Huaihe HospitalHenan UniversityKaifeng475000China Guangxi Key Laboratory of Molecular Medicine in liver Injury and Repair The Affiliated Hospital of Guilin Medical UniversityGuilin541001China

Objective Abnormal expression of T-lymphokine-activated killer cell-originated protein kinase(TOPK)was reported to be closely related to the resistance of prostate cancer to radiotherapy and to targeted drug resistance in lung ***,the role of TOPK inhibition in enhancing radiosensitivity of colorectal cancer(CRC)cells is *** study aimed to evaluate the radiosensitization of TOPK knockdown in CRC *** The expression of TOPK was detected in CRC tissues by immunohistochemistry,and the effect of TOPK knockdown was detected in CRC cells by Western ***-8 and clonogenic assays were used to detect the growth and clonogenic ability of CRC cells after TOPK knockdown combined with radiotherapy in CRC ***,proteomic analysis showed that the phosphorylation of TOPK downstream proteins changed after *** damage was detected by the comet *** in the DNA damage response signaling pathway were analyzed by Western blotting,and apoptosis was detected by flow *** The expression of TOPK was significantly greater in CRC tissues at grades 2–4 than in those at grade *** irradiation,CRC cells with genetically silenced TOPK had shorter comet tails and reduced expression levels of DNA damage response-associated proteins,including phospho-cyclin-dependent kinase 1(p-CDK1),phospho-ataxia telangiectasia-mutated(p-ATM),poly ADP-ribose polymerase(PARP),and meiotic recombination 11 homolog 1(MRE11).Conclusions TOPK was overexpressed in patients with moderately to poorly differentiated ***,TOPK knockdown significantly enhanced the radiosensitivity of CRC cells by reducing the DNA damage response.

关键词： T-lymphokine-activated killer cell-originated protein kinase colorectal cancer DNA damage response radiosensitivity

Depression of the Thoracolumbar Posterior Vertebral Body on the Estimation of Cement Leakage in Vertebroplasty and Kyphoplasty Operations

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Chinese Medical Journal 2015年第23期128卷 3158-3162页

作者： Hao Chen Pu Jia li Bao fei Feng he Yang Jin-Jun li Hai Tang Department of Orthopaedics Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Background： The cross-section ofthoracolumbar vertebral body is kidney-shaped with depressed posterior boundary. The anterior wall of the vertebral canal is separated from the posterior wall of the vertebral body on the lateral X-ray image. This study was designed to determine the sagittal distance between the anterior border of the vertebral canal and the posterior border of the vertebral body （DBCV） and to analyze the potential role of DBCV in the estimation of cement leakage during percutaneous vertebroplasty （PVP） or percutaneous kyphoplasty （PKP）. Methods： We retrospectively recruited 233 patients who had osteoporotic vertebral compression fractures and were treated with PVP or PKP. Computed tomography images of T11-L2 normal vertebrae were measured to obtain DBCV. The distance from cement to the posterior wall of the vertebral body （DCPW） of thoracolumbar vertebrae was measured from C-arm images. The selected vertebrae were divided into two groups according to DCPW, with the fracture levels, fracture grades and leakage rates of the two groups compared. A relative operating characteristic （ROC） curve was applied to determine whether the DCPW difference can be used to estimate the degree of cement leakage. The data were processed by statistical software SPSS version 21.0 using independent sample t-test and Chi-square tests. Results： The maximum DBCV was 6.40 mm and the average DBCV was 3.74 ± 0.95 mm. DBCV appeared to be longer in males than in females, but the difference was not statistically significant. The average DCPW of type-B leakage vertebrae （2.59 ±1.20 ram） was shorter than that of other vertebrae （7.83 ±2.38 mm, P 〈 0.001）. The leakage rate of group DCPW ≤6.40 mm was lower than that of group DCPW 〉6.40 mm for type-C and type-S, but much higher for type-B. ROC curve revealed that DCPW only has a predictive value for type-B leakage （area under the curve： 0.98, 95% confidence interval： 0.95-0.99, P 〈 0.001）, and when the cut-ott＇value was 4.05 mm, the di

关键词： C-arm Image Complications Kyphoplasty Vertebrae Vertebroplasty

应力对La_(0.83)Sr_(0.17)MnO_3薄膜输运性能的影响

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物理学报 2006年第3期55卷 1435-1440页

作者：江阔李合非宫声凯北京航空航天大学材料学院北京100083

采用溶胶-凝胶方法在Si(111)上制备了LSMO(x=0.17)薄膜.研究了块体材料和不同厚度薄膜R-T曲线、红外光谱和X射线衍射.结果表明,LSMO薄膜属于正交晶体结构,薄膜取向与膜厚度有关,当膜厚度为450nm或680nm时,主要取向〈200〉,而膜厚度为90... 详细信息

采用溶胶-凝胶方法在Si(111)上制备了LSMO(x=0.17)薄膜.研究了块体材料和不同厚度薄膜R-T曲线、红外光谱和X射线衍射.结果表明,LSMO薄膜属于正交晶体结构,薄膜取向与膜厚度有关,当膜厚度为450nm或680nm时,主要取向〈200〉,而膜厚度为900nm时取向为〈020〉:根据离子对相互作用能和谐振子模型,得到了红外吸收与Mn—O—Mn键长和键角关系式,600cm-1附近红外吸收与晶格常数b的变化有关;块体与薄膜的金属—绝缘体转变温度(TMI)存在较大差别,薄膜转变温度显著低于块体,并与厚度有一定关系.认为是LSMO薄膜中的应力诱导了晶格常数变化,引起键角改变及JT效应是转变温度变化的主要原因.

关键词：单晶硅晶格常数金属-绝缘体转变温度应力诱导

An efficient quantum proactive incremental learning algorithm

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 49-57页

作者： lingxiao li Jing li Yanqi Song Sujuan Qin Qiaoyan Wen fei Gao State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications School of Cyberspace Security Beijing University of Posts and Telecommunications

In scenarios where a large amount of data needs to be learned, incremental learning can make full use of old knowledge, significantly reduce the computational cost of the overall learning process, and maintain high performance. In this paper, taking the MaxCut problem as our example, we introduce the idea of incremental learning into quantum computing, and propose a Quantum Proactive Incremental Learning algorithm(QPIL). Instead of a one-off training of quantum circuit, QPIL contains a multi-phase training on gradually-increased subgraphs of all vertices, proactively reducing large-scale problems to smaller ones to solve in steps, providing an efficient solution for MaxCut. Specifically, some vertices and corresponding edges are randomly selected for training to obtain optimized parameters of the quantum circuit at first. Then, in each incremental phase, the remaining vertices and corresponding edges are gradually added and the parameters obtained from the previous phase are reused in the parameter initialization of the current phase. We perform experiments on 120 different small-scale graphs, and it shows that QPIL performs superior to prevalent quantum and classical baselines in terms of approximation ratio(AR), time cost, anti-forgetting, and solving stability. In particular, QPIL's AR surpasses 20% of mainstream quantum baselines, and the time cost is less than 1/5 of them. The idea of QPIL is expected to inspire efficient and high-quality solutions in large-scale Max Cut and other combinatorial optimization problems.

关键词： variational quantum algorithm incremental learning multi-phase training MaxCut quantum computing

GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

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Cell Research 2001年第2期11卷 161-163页

作者： JIN XIAO PING, FANG HUANG, NA YANG, BU FENG LU, JIAN fei, li he GUO Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai.

GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5’ Rapid amplification of cDNA end (RACE) was used to determine GAT1 transcriptional starting sites in neonatal mouse cerebral cortex and intestine, adult mouse brain and adult rat testis. The products of 5’RACE were confirmed by DNA sequencing. We found that the transcript of GAT1 in neonatal mouse cerebral cortex and adult mouse brain starts at the same site (inside of exon 1), while in mouse intestine, GAT1 starts transcription in intron 1, and in rat testis, the transcript of GAT1 has an additional untranslation exon to the 5’ direction.

关键词： GABA transporter 1(GAT1) transcriptional starting site 5’ RACE tissue spedcific difference DNA sequencing.

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傅里叶函数前缘仿生压气机叶栅性能研究

工程热物理学报 2020年第12期41卷 2936-2941页

作者：李润泽李赫飞姜斌郑群何宏伟哈尔滨工程大学动力与能源工程学院哈尔滨150001 中国航空工业空气动力研究院哈尔滨150001

本文针对仿鲸鳍前缘造型在压气机叶栅中的应用,提出了一种基于傅里叶级数形式的复合函数前缘造型方法。采用数值模拟方法,重点分析了零攻角下仿鲸鳍叶栅流场结构的变化,给出了不同仿鲸鳍前缘在叶栅端区以及叶中部损失抑制的原因和前缘... 详细信息

本文针对仿鲸鳍前缘造型在压气机叶栅中的应用,提出了一种基于傅里叶级数形式的复合函数前缘造型方法。采用数值模拟方法,重点分析了零攻角下仿鲸鳍叶栅流场结构的变化,给出了不同仿鲸鳍前缘在叶栅端区以及叶中部损失抑制的原因和前缘型线特征,优化出一种具有展向非均匀鳍型前缘的仿生叶栅,对非均匀鳍叶栅的性能进行了验证。研究结果表明,仿鲸鳍叶栅在前缘凹陷处形成流向涡对,在凸起处收缩流动形成高能气团,上述流场结构交替出现,抑制了角区及叶中部的分离流动,降低了叶栅的总压损失。基于高阶傅里叶函数的非均匀鳍型能更好地适应叶栅的变攻角特性,非均匀鳍叶栅总压损失系数相比原始叶栅降低了11.45%。

关键词：压气机叶栅仿鲸鳍前缘造型气动性能

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu Xiao-li li Xiao-Ting Sun li-Ting Wang Xia Wu ling-Jie Zhu Xiao-Jia Yang Qian-Ru he fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Mn的价态对La_(0.8)Ba_(0.2)MnO_3电磁性能的影响

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物理学报 2005年第9期54卷 4374-4378页

作者：江阔李合非马文宫声凯北京航空航天大学材料学院北京100083

在2—390K温度之间研究了La0.8Ba0.2MnO3的磁矩、磁电阻与温度的关系.发现以不同价态的Mn元素引入得到的La0.8Ba0.2MnO3,性能虽然都存在金属—绝缘体转变,以及在磁场作用下居里温度附近电阻率变化非常显著的特点,但是价态对磁性转变温度... 详细信息

在2—390K温度之间研究了La0.8Ba0.2MnO3的磁矩、磁电阻与温度的关系.发现以不同价态的Mn元素引入得到的La0.8Ba0.2MnO3,性能虽然都存在金属—绝缘体转变,以及在磁场作用下居里温度附近电阻率变化非常显著的特点,但是价态对磁性转变温度TC,金属—绝缘体转变温度Tmi,以及磁电阻极大值温度TMR的影响都非常显著.三种价态相比较,使用二价Mn的电阻率最低以及磁性转变温度更接近室温.认为影响材料性能的主要因素是材料制备时引进的Mn元素的价态,由于原料价态的不同而形成的氧空位浓度变化,进而影响了Mn4+Mn3+的比.

关键词：价态磁电阻居里温度金属—绝缘体转变 Mn^3+ 不同价态电磁性能转变温度磁场作用材料制备材料性能浓度变化

基于布鲁姆过滤器的面向IP包识别的CPBF算法

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华南理工大学学报（自然科学版） 2017年第7期45卷 90-97,106页

作者：李龙飞贺占庄史阳春西安微电子技术研究所集成电路设计部陕西西安710065

针对现有布鲁姆过滤器在流识别应用中对每个IP包进行相同的处理,未考虑IP包识别失效代价和硬件开销的问题,提出一种面向IP包识别的算法——CPBF(Classified and Pipelined Bloom Filter).该算法通过引入IP头中服务类型作为识别失效代价... 详细信息

针对现有布鲁姆过滤器在流识别应用中对每个IP包进行相同的处理,未考虑IP包识别失效代价和硬件开销的问题,提出一种面向IP包识别的算法——CPBF(Classified and Pipelined Bloom Filter).该算法通过引入IP头中服务类型作为识别失效代价的判断依据对IP包进行分类,根据分类结果采取不同数目的 Hash函数进行映射,降低高失效代价IP包的识别失效率;同时在Hash计算中采用流水机制加速识别速率;基于概率论、微分方程等相关知识对CPBF算法进行了描述和理论分析,最后在FPGA上对算法进行实现和实验.结果表明,与标准布鲁姆过滤器、多维布鲁姆过滤器相比,CPBF在具有较低的识别失效率和硬件开销的同时,也能保持较高的识别速率.

关键词：布鲁姆过滤器 CPBF算法 IP包识别识别失效代价 Hash函数