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Expressions of PCNA,p53,p21^(WAF-1)and cell proliferation in fetal esophageal epithelia:Comparative study with adult esophageal lesions from subjects at high-incidence area for esophageal cancer in Henan,North China

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World Journal of Gastroenterology 2003年第7期9卷 1601-1603页

作者： YingXing YuNing li-QiangRu li-dongwang DepartmentofNeurobiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina DepartmentofPhysiology CollegeofMedicineZhengzhouUniversityZhengzhou450052HenanProvinceChina LaboratoryforC'ancerResearch CollegeofMedicineZhengzhouUniversityZhc~gzhou450052HenanProvinceChina

AIM: To characterize the expression of p53, p21WAF-1 and proliferation-cell-nuclear-antigen (PCNA) in fetal esophageal epithelia and to determine the role of these genes in proliferation of fetal and adult esophageal epithelial cells. METHODS: Immunohistochemical avdin-biotin peroxidase complex (ABC) method was applied to 31 cases of fetal esophageal specimens and 194 cases of adult esophageal specimens to detect the expression of p53, p21WAF-1 and PCNA in fetal and adult esophageal epithelia. RESISTS: Both the PCNA positive immunostaining cell number and PCNA positive immunostaining rate in fetal esophageal epithelia (5064-239) were significantly higher than those in adults, induding normal epithelia (2004-113) and epithelia with basal cell hyperplasia (BCH) (2864-150) (Plia (5064-239) (Plia, which was significantly lower than that in adult normal esophageal epithelia (50 %), adult epithelia with basal cell hyperplasia (62 %), dysplasia (73 %) and squamous cell carcinoma (86 %) (Plia. However, p21w^l positive immunost^ining cells wereobserved in adult esophagus with 39 % (11/28) in normal, 38% (14/37) in BCH, 27 % (3/11) in DYS and 14 % (1/7) in SCC. CONCLUSION: PCNA could act as an indicator accurately reflecting the high proliferation status of fetal esophageal epithelium, p53 may play an important role in growth and differentiation of fetal esophageal epithelium, p21WAF-1 may have no physiological function in development of fetal esophageal epithelium.

关键词： PCNA p53 p21^WAF-1 细胞增殖胚胎食管上皮食管癌

Effects of Yogurt Supplementation on the Growth of Preschool Children in Beijing Suburbs

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Biomedical and Environmental Sciences 2005年第3期18卷 192-197页

作者： MEIHE YUE-XINYANG HuIHAN JIAN-HUAMEN li-HUABIAN GUO-dongwang InstituteofNutritionandFoodSafety ChineseCenterforDiseaseControlandPreventionBeijing100050China

Objective To investigate the effect of yogurt supplementation on the growth of preschool children in Beijing suburbs. Methods Four hundred and two preschool children (217 males, 185 females), aged 3-5 years, whose height for age and/or weight for age were less than the reference level, were selected as subjects from 7 kindergartens in Beijing Fangshan District. The subjects were divided randomly into control group (CG, 201) and yogurt supplemented group (YG, 201). Each subject in YG was given one serving of yogurt (125 g) for 5 days a week from March to December in 2001, while nothing additional was provided to CG. All subjects kept their usual diet during the study. Anthropometry (body height and weight and upper-arm circumference) and the bone mineral density (BMD) of forearm were measured every 3 months. Disease status and dietary intake were also recorded and assessed. Results The intake of calcium, zinc, and vitamin B2 in YG was significantly higher than that in CG. The incidence and duration of upper-respiratory infection and diarrhea of children in YG were significantly less than those in CG. The height gain of children in YG was significantly higher than that in CG after yogurt was supplemented for 3, 6, and 9 months (P<0.05) (1.90±0.49 cm vs 1.77±0.54 cm, 3.83±0.57cm vs 3.64±0.66 cm and 5.43±0.69 cm vs 5.24±0.76 cm, respectively). The weight gain of children in YG was significantly higher than that in CG after yogurt was supplemented for 3, 6, and 9 months (P<0.05) (0.70±0.43 kg vs 0.49±0.35 kg, 0.98±0.62 kg vs 0.80±0.60 kg and 1.42±0.76 kg vs 1.20±0.67 kg, respectively). The BMD of children in YG was significantly higher than that in CG after yogurt was supplemented for 9 months (P<0.05) (0.415±0.058 g/cm2 vs 0.400±0.065 g/cm2). Conclusion Yogurt is beneficial to the improvement of calcium, zinc, and vitamin B2 intake, the decreasing of the incidence and duration of upper-respiratory infection and diarrhea, and the promotion of the health and the growth an

关键词： Yogurt Preschool children Growth and development

CYP1A1,GSTs and mEH polymorphisms and susceptibility to esophageal carcinoma:Study of population from a high-incidence area in north China

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World Journal of Gastroenterology 2003年第7期9卷 1394-1397页

作者： Jian-XiangZhou Yan-Jieli Ji-Xueli li-dongwang ShuZheng Binliu LaboratoryforCancerResearch CollegeofMedicineZhengzhouUniversityZhengzhou450052HenanProvinceChina CancerInstitute ZhejiangUniversityHangzhou310009ZhejiangProvinceChinaandLaboratoryforCancerResearchCollegeofMedicineZhengzhouUniversityZhengzhou450052HenanProvinceChina CancerInstitute ZhejiangUniversityHangzhou310009ZhejiangProvinceChina DepartmentofGastroenterology TongrenHospitalCapitalMedicalUniversityBeijing100013China

AIM: To characterize cytochrome P4501A1 (CYPIA1), glutathione S-transferases (GSTs) and microsomal epoxide hydrolase (mEH) polymorphisms in Chinese esophageal cancer patients. METHODS: Multiplex polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphisms (PCRRFLP) were used to detect polymorphism changes of CYP,GSTs and mEH on esophageal cancerous and precancerous lesions as well as in case control group. All the examination samples were obtained from linzhou (formerly linxian), Henan Province, the highest incidence area for esophageal. RESULTS: The frequency of CYP1A1 3'' polymorphism in case control group (26/38, 68 %) was significantly higher than in esophageal squamous cell carcinoma^roup (ESCC) (29/62, 47 %) (Plity to esophageal cancer, mEH slow allele variant,associated with the progression of esophageal precancerous lesions, may conthbute to the high susceptibility to esophageal carcinoma.

关键词： CYPIA1 GSTs mEH 基因多态性食道癌聚合酶链反应

Multifactor roadmap for designing low-power-consumed micro thermoelectric thermostats in a closed-loop integrated 5G optical module

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Interdisciplinary Materials 2024年第2期3卷 326-337页

作者： dongwang Yang Yubing Xing Jiang Wang Kai Hu Yani Xiao Kechen Tang Jianan Lyu Junhao li Yutian liu Peng Zhou Yuan Yu Yonggao Yan Xinfeng Tang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhanChina Nanostructure Research Center Wuhan University of TechnologyWuhanChina Research Center for Materials Genome Engineering Wuhan University of TechnologyWuhanChina Institute of Physics(IA) RWTH Aachen UniversityAachenGermany

As the core components of fifth-generation(5G)communication technology,optical modules should be consistently miniaturized in size while improving their level of *** inevitably leads to a dramatic spike in power consumption and a consequent increase in heat flow density when operating in a confined *** ensure a successful start-up and operation of 5G optical modules,active cooling and precise temperature control via the Peltier effect in confined space is essential yet *** this work,p-type Bi_(0.5)Sb_(1.5)Te_(3)and n-type Bi_(2)Te_(2.7)Se_(0.3)bulk thermoelectric(TE)materials are used,and a micro thermoelectric thermostat(micro-TET)(device size,2×9.3×1.1mm^(3);leg size,0.4×0.4×0.5mm^(3);number of legs,44)is successfully integrated into a 5G optical module with Quad Small Form Pluggable 28 *** a result,the internal temperature of this kind of optical module is always maintained at 45.7°C and the optical power is up to 7.4 ***,a multifactor design roadmap is created based on a 3D numerical model using the ANSYS finite element method,taking into account the number of legs(N),leg width(W),leg length(L),filling atmosphere,electric contact resistance(Rec),thermal contact resistance(Rtc),ambient temperature(Ta),and the heat generated by the laser source(QL).It facilitates the integrated fabrication of micro-TET,and shows the way to enhance packaging and performance under different operating *** to the roadmap,the micro-TET(2×9.3×1mm^(3),W=0.3 mm,L=0.4 mm,N=68 legs)is fabricated and consumes only 0.89W in cooling mode(Q_(L)=0.7W,T_(a)=80℃)and 0.36Win heating mode(T_(a)=0℃)to maintain the laser temperature of 50℃.This research will hopefully be applied to other microprocessors for precise temperature control and integrated manufacturing.

关键词： 5G optical module finite element simulation micro thermoelectric thermostat Peltier effect

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道路桥梁施工钢纤维混凝土技术应用

中国高新科技 2023年第4期 137-138,141页

作者：李东望中交二公局第二工程有限公司陕西西安710000

文章根据现有工程案例对道路桥梁施工过程中的纤维混凝土技术进行分析,并提出适用性策略,其中包括对其进行设计、接缝、搅拌、浇筑、振捣、成型,对桥梁的加固和桥面的铺装。通过研究得出结论,该技术完全可以满足施工需求,其工艺流程简单... 详细信息

文章根据现有工程案例对道路桥梁施工过程中的纤维混凝土技术进行分析,并提出适用性策略,其中包括对其进行设计、接缝、搅拌、浇筑、振捣、成型,对桥梁的加固和桥面的铺装。通过研究得出结论,该技术完全可以满足施工需求,其工艺流程简单,在保证施工质量的同时对车辆安全通行的影响较小,能够有效地节约成本。

关键词：道路桥梁施工钢纤维混凝土技术浇筑振捣加固处理

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浅析信息技术教学的基本方法

承德职业学院学报 2006年第2期11卷 126-128页

作者：李东旺承德民族师专附中河北承德067000

随着社会信息化程度的加深,信息技术在工作、学习、生活中的应用也越来越普遍了.信息社会所需要的人才必须具有良好的信息意识和信息能力,基础教育应承担起培养这种素质的职责.

关键词：信息技术课程中小学教学方法计算机学习兴趣认识兴趣

Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province

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World Journal of Gastroenterology 2004年第22期10卷 3365-3368页

作者： Ji-YeAn Zong-MinFan Ze-HaoZhuang Yan-RuQin Shah-ShahGao Ji-linli li-dongwang LaboratoryforCancerResearchandtheThirdTeachingHospital CollegeofMedicineZhengzhouUniversityZhengzhou450052HenanProvinceChina LaboratoryforCancerResearch CollegeofMedicineZhengzhouUniversityZhengzhou450052HenanProvinceChina DepartmentofPathology YaocunEsophagealCancerHospitalLinzhou456592HenanProvinceChina

AIM: To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at Me high-incidence area for ESCC in Henan Province, China. METHODS: Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation. RESULTS: A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between Me normal group and ESCC patients. A total of seven protein spots were identified wi~ a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin. CONCLUSION: Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of ~ese proteins needs to be further characterized. The present study provides informative data for Me establishment of serum protein profiles related with ESCC.

关键词：蛋白分解 ESCC 血液水平诊断方法食道鳞状细胞癌肿瘤高入射范围免疫血清

口腔鳞状细胞癌细胞周期蛋白D1的表达与意义

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天津医药 2003年第1期31卷 3-5页

作者：朱东望李世芬天津医科大学附属口腔医院 300070

目的:分析口腔鳞状细胞癌(OSCC)细胞周期蛋白D1(cyclin D1)的表达与临床病理资料及预后的关系。方法:回顾性研究50例OSCC及10例正常口腔黏膜cyclin D1的表达情况,采用SABC法根据染色指标计数,并利用Cox比例风险模型进行生存多因素分析... 详细信息

目的:分析口腔鳞状细胞癌(OSCC)细胞周期蛋白D1(cyclin D1)的表达与临床病理资料及预后的关系。方法:回顾性研究50例OSCC及10例正常口腔黏膜cyclin D1的表达情况,采用SABC法根据染色指标计数,并利用Cox比例风险模型进行生存多因素分析。结果:cyclin D1在正常口腔黏膜上皮呈现低水平表达,在OSCC组织中过表达。单因素生存分析表明cyclin D1的表达与生存期显著相关,多因素Cox回归分析表明cyclin D1的表达可作为OSCC辅助性的预后指标。结论:cyclin D1的表达与OSCC的发生发展有密切关系,并与肿瘤的预后密切相关,可作为辅助性的预后观察指标。

关键词：口腔肿瘤鳞状细胞癌免疫组织化学细胞周期蛋白D1 预后

Semi-automated detection of rangeland runoff and erosion control berms using high-resolution topography data

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International Soil and Water Conservation Research 2024年第1期12卷 217-226页

作者： li li Changjiang River Scientific Research Institute of Changjiang Water Resources Commission Wuhan430010China Research Center on Mountain Torrent&Geologic Disaster Prevention Ministry of Water ResourcesWuhan430010China University of Arizona School of Natural Resources and the EnvironmentTucsonAZ85719USA

An inventory of topographic modifications is essential to addressing their impacts on hydrological and morphological processes in human-altered ***,such inventories are generally *** study presents two workflows for semi-automatic detection of linear earthen runoff and erosion control berms in rangelands using high-resolution topographic *** workflows consist of initial object identification by applying either morphological grayscale reconstruction(MGR)or the Geomorphon(GEO)method,followed by identification refinements through filters based on objects’horizontal and vertical *** sites were selected within the Altar Valley,Arizona,in the southwestern United *** site was used for developing workflows and optimizing filter thresholds,and the other two sites were used to validate *** results showed that:1)The MGR-based workflow methodology could produce final precision and detection rates of up to 92%and 75%,respectively,and take less than 5 s for a 10.1 km^(2) site;2)The workflow based on the MGR method yielded greater identification accuracy than did the GEO workflow;3)Object length,orientation,and eccentricity were important characteristics for identifying earthen berms,and are sensitive to general channel flow direction and berm shape;4)Manual interrogation of topographic data and imagery can significantly improve identification precision *** proposed workflows will be useful for developing inventories of runoff and erosion control structures in support of sustainable rangeland management.

关键词： Earthen berm Rangeland Grayscale morphological reconstruction Geomorphon liDAR