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Synergistic responses of NHX, AKT1, and SOS1 in the control of Na^+ homeostasis in sweet sorghum mutants induced by ^(12)C^(6+)-ion irradiation

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Nuclear Science and Techniques 2018年第1期29卷 32-38页

作者： Wen-Ting Gu li-bin zhou Rui-Yuan liu Wen-Jie Jin Ying Qu Xi-Cun Dong Wen-Jian li Institute of Modern Physics Chinese Academy of Sciences

Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions of the synergistic responses of NHX, AKT1, and SOS1 related to Na^+ accumulation were investigated in control(KFJT-CK) sorghum and KF1210-3 and KF1210-4 mutants. The results indicated that the NHX, AKT1, and SOS1 proteins in sweet sorghum are mainly involved in the transport, exclusion, and spatial distribution of Na^+,respectively. In addition to physiological parameters, we also measured the expression levels of NHX, AKT1, and SOS1 genes. The experimental results indicated that 150 m M Na Cl induced marked increases in the transcripts of NHX and SOS1 after 8 and 12 h in the KF1210-3,KF1210-4, and KFJT-CK cultivars. In contrast, however, a decrease in AKT1 was observed. On the basis of our results, we propose a model in which cooperation amongNHX, AKT1, and SOS1 facilitates Na^+ homeostasis in sweet sorghum in response to an increase in salt concentration. Accordingly, study of the regulatory mechanisms in sweet sorghum generated by carbon ion irradiation is essential for the selection of salt-tolerant cultivars.

关键词： 12C6+-ion irradiation Sweet sorghum Salt stress NHX AKT1 SOS1

Endoscopic clipping for the secondary prophylaxis of bleeding gastric varices in a patient with cirrhosis:A case report

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World Journal of Clinical Cases 2022年第4期10卷 1447-1453页

作者： Guang-Chao Yang Ya-Xian Mo Wei-Hua Zhang li-bin zhou Xu-Ming Huang li-Ming Cao Department of Gastroenterology Shenzhen Shiyan People's HospitalShenzhen 518000Guangdong ProvinceChina Department of Internal Medicine Shenzhen University The First Affiliated HospitalShenzhen 518000Guangdong ProvinceChina

BACKGROUND Bleeding from gastroesophageal varices(GOV)is a serious complication in patients with liver cirrhosis,carrying a very high mortality *** secondary prophylaxis against initial and recurrent bleeding,endoscopic therapy is a critical *** variceal clipping for secondary prophylaxis in adult GOV has not been *** SUMMARY A 66-year-old man with cirrhosis was admitted to our hospital complaining of asthenia and hematochezia for 1 *** hemoglobin level and red blood cell counts were significantly decreased,and his fecal occult blood test was *** enhanced computed tomography of the abdomen showed *** patient was diagnosed with hepatitis B cirrhosis-related GOV bleeding.A series of palliative treatments were administered,resulting in significant clinical ***,an endoscopic examination revealed severe gastric fundal varices,prompting endoscopic variceal *** were no further episodes of gastrointestinal *** GOV improved significantly on follow-up imaging and was confirmed as improved on endoscopy at the 5th post-operative *** Our results suggest that endoscopic clipping is an inexpensive,safe,easy,effective,and tolerable method for the secondary prophylaxis of bleeding from gastric type 2 ***,additional research is indicated to confirm its longterm safety and efficacy.

关键词： Endoscopy Metal clips Gastric varices Endoscopic variceal clipping Secondary prophylaxis Case report

Morphological study on colonic pathology in ulcerative colitis treated by moxibustion

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World Journal of Gastroenterology 2000年第6期6卷 861-865页

作者： Huan Gan Wu li bin zhou Da Ren Shi Shi Min liu Hui Rong liu Bi Meng Zhang Han Ping Chen lin Shan Zhang Shanghai Institute of Acupuncture-Moxibustion and Meridians Shanghai 200030China Tumor Hospital Shanghai Medical UniversityShanghai 200032China Zhejiang Traditional Chinese Medicine College Hangzhou 310009Zhejiang ProvinceChina

AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal *** Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine *** were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the *** was also analyzed by H.E and *** Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion *** the control group,5patients were clinically cured,7 improved and *** patients with active UCwere taken as the subjects for histopathologicanalysis in this *** colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also *** sections,the chronic inflammation ofmucosa was geatly ameliorated(Plitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal.

关键词： colitis ulcerative/therapy moxibustion intestinal mucosa morphological study

A novel adaptive spectrum forming filter:Application in cognitive ultra-wideband

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Science China(Information Sciences) 2010年第12期53卷 2584-2599页

作者： li bin ,zhou Zheng & ZOU WeiXia Key Lab of Universal Wireless Communications,MOE.Wireless Network Lab,Beijing University of Posts and Telecommunications,Beijing 100876,China Key Lab of Universal Wireless Communications MOE. Wireless Network Lab Beijing University of Posts and Telecommunications Beijing China

Ultra wideband impulse radio(UWB-IR) has important applications in high data rate communications,high precision positioning and penetrating target *** with cognitive radio(CR),UWB can also greatly alleviate the current spectrum ***,the existing UWB waveforms are faced with two *** spectrum utilization of UWB signal is rather low,which reduces the communication ***,it appears difficult for UWB-IR to avoid spectrum collision with other incumbent *** UWB will inevitably interfere other legal wireless *** by these considerations,we model the UWB waveform design into an interpolation *** on radial basis function neural network,a novel UWB pulse is presented in this *** UWB pulse is then realized though reasonable simplification on the already proposed theoretical *** designed UWB pulses can adaptively track the external spectral environment where UWB devices work and reconfigure its emission spectrum according to the state of primer users without any modification on *** frequency utilization is maximized when there is no active primary *** adequate spectrum avoidance has also been achieved without much spectrum utilization degradation within the non-primary *** and analysis show that our UWB-IR pulse is much superior to the other existing UWB signals in the frequency utilization,spectrum avoidance and in the transmission performance.

关键词： UWB-IR cognitive radio pulse design RBF network spectrum efficiency spectrum avoidance

RPPK modulation with high data rates

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Science China(Information Sciences) 2010年第2期53卷 344-354页

作者： li bin , zhou Zheng & ZOU WeiXia Key Laboratory of Universal Wireless Communications, Ministry of Education Wireless Network Laboratory, Beijing University of Posts and Telecommunications, Beijing 100876, China Key Laboratory of Universal Wireless Communications Ministry of Education Beijing University of Posts and Telecommunications Beijing China Wireless Network Laboratory Beijing University of Posts and Telecommunications Beijing China

With the development of communication technology, the precious frequency spectrum is becoming more and more crowded. Exploiting the particular filter with zero group delay, ultra narrow band (UNB) modulation is intended for acquiring the high frequency efficiency. Unfortunately, this UNB filter is confronted with great challenges from the classical communication theories. The validation on its realization is also difficult. This paper proposes a novel UNB modulation, namely random pulse position keying (RPPK). It is demonstrated from analysis that there is no inessential discrete spectral line in modulated signal’s power density spectrum (PDS), and the UNB filter has been successfully avoided. Consequently, RPPK is rather explicit in theory and simple in implementation. As is shown by the simulation, the frequency utilization of RPPK can even reach 100 bits/s/Hz. Its optimum receiving performance is slightly inferior to BPSK, yet is much superior to that of other UNB modulations such as VMSK/2. Moreover, multi-access and confidentiality are additional benefits to UNB users.

关键词： UNB modulation frequency utilization efficiency RPPK PDS

Modified vitrification method for cryopreservation of human ovarian tissues

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Chinese Medical Journal 2007年第2期120卷 110-114页

作者： li Yu-bin zhou Can-quan YANG Guo-fen WANG Qiong DONG Yu Center of Reproductive Medicine First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 China

Background Vitrification is a prospective technology in ovarian tissue cryopreservation, but it is still in an initial stage. This study was conducted to investigate a modified vitrification protocol for human ovarian tissue, which can be used as an alternative to preserve fertility for young women with cancer who have to undergo cytotoxic therapy and sterilization. Methods Ovarian tissue samples were collected from 15 patients and randomly allocated to groups of fresh, vitrification, and conventional slow freezing. A modified carrierless vitrification method was applied. The proportion of morphologically intact follicles in fresh ovarian tissues was compared with that in warmed/thawed tissues. The initial growth of the follicles and the concentrations of estradiol and progesterone were detected to determine the viability and endocrine function of the cryopreserved tissues. Results The proportion of morphologically intact primordial follicles in the fresh group （97.6%） was significantly higher than that in the other two groups （vitrification group 80.3% and slow-freezing group 72.6%, P〈0.001）. In both the vitrification and slow-freezing groups, estradiol and progesterone were secreted continuously during 2-week culture in vitro, the proportion of primary follicles were both significantly increased compared to the fresh group. No statistically significant differences existed between the two groups after cryopreservation in the proportion of both primordial and primary follicles, and the concentrations of estradiol and progesterone （P〉0.05）.Conclusion The modified vitrification method for cryopreservation of human ovarian tissues is effective, simple, and inexpensive.

关键词： cryopreservation vitrification ovarian tissue

Expression of feeding-related peptide receptors mRNA in GT1-7 cell line and roles of leptin and orexins in control of GnRH secretion

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Acta Pharmacologica Sinica 2005年第8期26卷 976-981页

作者： Ying YANG, li-bin zhou, Shang-quan liU, Jing-feng TANG, Feng-yin li, Rong-ying li, Huai-dong SONG, Ming-dao CHEN~2 Rui-jin Hospital Affiliated to Shanghai Second Medical University, Shanghai Institute of Endocrine and Metabolic Diseases, Shangha 200025. China Rui-jin Hospital Affiliated to Shanghai Second Medical University Shanghai Institute of Endocrine and Metabolic Diseases Shanghai China

Aim: To investigate the expression of feeding-related peptide receptors mRNA in GT1-7 cell line and roles of leptin and orexins in the control of GnRH secretion. Methods: Receptors of bombesin3, cholecystoldnin (CCK)-A, CCK-B, glucagonlike peptide (GLP)1, melanin-concentrating hormone (MCH)I, orexin1, orexin2, neuromedin-B, neuropeptide Y (NPY)1 and NPYS, neurotensin (NT)1, NT2, NT3, and leptin receptor long form mRNA in GT1-7 cells were detected by reversed transcriptase-polymerase chain reaction. GT1-7 cells were treated with leptin, orexin A and orexin B at a cohort of concentrations for different lengths of time, and GnRH in medium was determined by radioimmunoassay (RIA). Results: Receptors of bombesin 3, CCK-B, GLP1, MCH1, orexin1, neuromedin-B, NPY1, NPYS, NT1, NT3, and leptin receptor long form mRNA were expressed in GT1-7 cells, of which, receptors of GLP1, neuromedin-B, NPY1, and NT3 were highly expressed. No amplified fragments of orexin2, NT2, and CCK-A receptor cDNA were generated with GT1-7 RNA, indicating that the GT1-7 cells did not express mRNA of them. Leptin induced a significant stimulation of GnRH release, the results being most significant at 0.1 nmol/L for 15 min. In contrast to other studies in hypothalamic explants, neither orexin A nor orexin B affected basal GnRH secretion over a wide range of concentrations ranging from 1 nmol/L to 500 nmol/Lat 15, 30, and 60 min. Conclusion: Feeding and reproductive function are closely linked. Many orexigenic and anorexigenic signals may control feeding behavior as well as alter GnRH secretion through their receptors on GnRH neurons.

关键词： GT1-7 cell line bombesin cholecystokinin neuropeptide Y neurotensin orexin leptin gonadorelin

Metal nanoparticles decorated CoFe-(oxy)hydroxysulfides nanosheets fabricated by a general strategy for electrocatalytic water splitting

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Journal of Energy Chemistry 2025年第1期100卷 26-38页

作者： Xiaodong Yang Haochen Shen Xiaoming Xiao Zhichao li Qi zhou Wei Yang bin Jiang Yongli Sun Luhong Zhang Zhenhua Yan School of Chemical Engineering and Technology Tianjin University Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

This study presents a novel method to fabricate metal-decorated,sulfur-doped layered double hydroxides （M/SLDH） through spontaneous redox and sulfurization *** developed Ag/SLDH and Pt/SLDH catalysts with abundant heterogeneous interfaces and hierarchical nanostructures demonstrated outstanding oxygen evolution reaction （OER） and hydrogen evolution reaction （HER） performance,achieving low overpotentials of 212 and 35 mV at 10 mA cm-2in 1 M KOH,*** both anode and cathode in water splitting,they required only 1.47 V to reach 10 mA cm-2and exhibited high structural robustness,maintaining stability at 1000 mA cm-2for 300 ***-situ Raman analysis revealed that the synergistic effects of metal nanoparticles and S doping significantly promote the transformation into the S-Co1-xFexOOH layer,which serves as the active phase for water ***,ultraviolet photoelectron spectroscopy （UPS） and density functional theory （DFT） analyses indicated that incorporating metal nanoparticles and S doping increase electron density near the Fermi level and reduce reaction energy barriers,thus enhancing intrinsic OER and HER *** study provides a scalable strategy for synthesizing high-performance electrocatalysts for water splitting,with promising potential for broader applications.

关键词： LDH Spontaneous reaction Metal nanoparticles Water splitting

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高眼压症的相关特殊检查研究

国际眼科杂志 2013年第7期13卷 1358-1359页

作者：陈丽周莅斌中国四川省攀枝花市中西医结合医院眼科 617000

高眼压症又称为疑似青光眼或早期青光眼,随着临床研究的深入,眼科检测技术不断提高,对高眼压症的诊断、治疗评估、判断预后等有重要指导意义。我们仅对近年来高眼压症的相关特殊检查研究进展作一总结。

关键词：高眼压症特殊检查