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Long-term thinning decreases the contribution of heterotrophic respiration to soil respiration in subalpine plantations

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Journal of Forestry Research 2024年第3期35卷 189-204页

作者： Longfei Chen Zhibin He Wenzhi Zhao Xi Zhu Qin Shen Mingdan Song zhengpeng li Junqia Kong Shuping Yang Yuan Gao Northwest Institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhou 730000People's Republic of China State Forestry Administration Key Laboratory of Forest Resources and Environmental Management Beijing Forestry UniversityBeijing 100083People's Republic of China Academy of Agriculture and Forestry Sciences Qinghai UniversityXining 810000People's Republic of China College of Landscape Architecture Zhejiang A&F UniversityHangzhou 310000People's Republic of China

Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental ***,as a principal silvicultural practice,the long-term impacts of thinning on R_(S) and its heterotrophic and autotrophic respiration components(R_(h) and Ra,respectively)in subalpine plantations are poorly understood,espe-cially in winter.A 3-year field observation was carried out with consideration of winter CO_(2) efflux in middle-aged sub-alpine spruce plantations in northwestern China.A trench-ing method was used to explore the long-term impacts of thinning on Rs,Rn and R_(a).Seventeen years after thinning,mean annual Rs,Rn and R_(a) increased,while the contribu-tion of R_(h) to R_(s) decreased with thinning *** significantly decreased winter R,because of the reduction in R_(n) but had no significant effect on *** temperature sensitivity(Q_(10))of R_(h) and R_(a) also increased with thinning intensity,with lower Q_(10) values for R_(h)(2.1-2.6)than for Ra(2.4-2.8).The results revealed the explanatory variables and pathways related to R_(n) and R_(a) *** increased soil moisture and nitrate nitrogen(NO_(3)^(-)-N),and the enhanced nitrogen and water availability promoted R_(h) and R_(a) by improving fine root biomass and microbial *** results highlight the positive roles of NO_(3)^(-)-N in stimulating R_(s) components following long-term ***,applications of nitrogen fertilizer are not recommended while thinning subalpine spruce plantations from the perspective of reducing soil CO_(2) *** increased Q_(10) values of R_(s) components indicate that a large increase in soil CO_(2) emissions would be expected following thinning because of more pronounced climate warming in alpineregions.

关键词： Heterotrophic respiration Autotrophic respiration Long-term thinning impacts Cold seasons Subalpine plantations Temperature sensitivity

Five Steps to Cultivate Volvariella volvacea

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Agricultural Science & Technology 2017年第9期18卷 1593-1594,1618页

作者： zhengpeng li Mingjie CHEN Changxia YU Qiaozhen li Feng ZHOU Yu li Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Key Laboratory of Edible Fungi Resources and Utilization(South) of Ministry of AgricultureP.R.China National Engineering Research Center of Edible Fungi

Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial *** the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting.

关键词： Volvariella volvacea Composting Spawning Pining Harvesting

High-yield Cultivating Techniques of Flammulina velutipes

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Agricultural Science & Technology 2017年第6期18卷 1010-1011,1015页

作者： Feng ZHOU Qiaozhen li Chunyan SONG zhengpeng li Qi TAN Xiaodong SHANG Yu li Institute of Edible Fungi Shanghai Academy of Agricultural Sciences/National Engineering Research Center of Edible Fungi/Key Laboratory of Edible Fungi Resources and Utilization (South) Ministry of Agriculture

Flammulina velutipes is an edible fungus with the largest amount of factory-like cultivation yield in China. In this study,based on many years of cultivation experience, 2 kinds of factory-like high-yield cultivating techniques of F. velutipes in China were summarized. One way is bag cultivation, and the other way is bottle cultivation. The cultivation process of the 2 methods were introduced in detail, including mixing material, bagging or bottling, sterilization, inoculation, spawn running, and mushroom fruiting.

关键词： Flammulina velutipes Factory-like cultivation Cultivation techniques

Key Technology for liquid Spawn Production and Green and High-yield Cultivation of Pleurotus eryngii

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Asian Agricultural Research 2020年第7期12卷 52-54页

作者： Yali ZHANG Shuguang ZHAO Yu li zhengpeng li Cuie ZHANG Xiaotao SUN Agricultural Technology Extension Center of Guannan County Guannan 222500 China Vegetable Office of Guannan County Guannan 222500 China Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201403 China Jiangsu lisha Fungus Industry Co. Ltd. Guannan 222500 China

[Objectives]Pleurotus eryngii is currently the second largest edible fungus variety cultivated in China,and it mainly adopts the bag cultivation *** study aims to effectively solve the problems of high cost of solid spawn,long spawn production cycle,low application level of green and high-yield cultivation technology and equipment,and unstable output and quality in the factory production process and to further promote industrial upgrading.[Methods]Since June,2018,a series of research and technical optimizations had been carried out by a technical research team consisting of personnels from Vegetable Office of Guannan County,Institute of Edible Fungi of Shanghai Academy of Agricultural Sciences and Jiangsu Xiangru Biotechnology Co.,Ltd.,around the production of liquid spawn,introduction of excellent varieties of ***,medium pretreatment,autoclaving,clean room inoculation,digital cultivation,harvesting,packaging,etc.[Results]The key technology of industrial production,liquid spawn production and green and high-yield cultivation of *** are summarized.[Conclusions]The economic benefit of liquid spawn of *** is extremely considerable compared with liquid spawns such as branch spawn.

关键词： Pleurotus eryngii liquid spawn Factory cultivation Green High yield

Study on the Variation of Three Parameters during Industrial Cultivation of liquid Spawn to Pleurotus eryngii

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Agricultural Biotechnology 2017年第6期6卷 57-59页

作者： Feng ZHOU Tao WANG Yu li Xiaodong SHANG Chunyan SONG Qiaozhen li Qi TAN zhengpeng li Institute of Edible Fungi Shanghai Academy of Agricultural Sciences/Key Laboratory of Edible Fungi Resources and Utilization( South) Ministry of Agriculture P. R. China/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Edible Fungi Resources and Utilization (South) Ministry of Agriculture P.R. China/Key Laboratory of Agricultural Genetics and Breeding of Shanghai Shanghai 201403 China

In order to effectively guide industrial cultivation of liquid spawn to Pleurotus eryngii, the changes of the mycelial amount, CO2 concentration and pH value during the industrial cultivation of liquid spawn to Pleurotus eryngii were studied. The results showed that during the culture of mycelium, the changes of my- celium amount and CO2 concentration accorded with the S type growth curve, and the 4^th -8^th d was the logarithmic phase with the growth amount of mycelium 0.05 g/100 ml per day. After 9 d, the mycelium growth tended to slow down, and by tile end of the mycelium cultivation, mycelium began to slightly decrease. The pH value of medium changed slightly in 0.5 during the mycelium cultivation. The three groups of parameters showed that the liquid strain fermentation time should be 8 to9d.

关键词： Pleurotus eryngii liquid spawn Mycelium amount COs concentration pH value

Pseudomonas *** Z0-J is a promising biocontrol agent on postharvest brown rot of apple

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Pseudomonas *** Z0-J is a promising biocontrol agent on post...

金针菇产木聚糖酶固态发酵条件优化和酶学性质研究

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： li zhengpeng Song Huwei Gu Yi’an Yang Wei Luo Yuming Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake School of Life ScienceHuaiyin Normal University

Apple brown rot,caused by Monilinia fructicola,results in a serious enormous loss during apple *** and more beneficial microorganisms are considered as potential biological *** this study,an endophytic Pseudomonas *** ZO-J was identified and the biocontrol effects on postharvest brown rot of apple were evaluated under laboratory conditions as *** on the library of beneficial microbial resources,we screened and identified the endophytic Pseudomonas *** ZO-J by colony characteristics,physiological and biochemical methods,16 S rDNA *** experiment in dishes indicated that the treatment with fermentation broth(FB)(0-,10-,20-,and 50-fold dilutions) inhibited mycelial growth by more than 90%.Moreover,FB still retained a significant inhibitory activity(about 50%) even after a 500-fold *** treatment also induced severe malformation and protoplasm releases compared with the normal *** all,FB significantly inhibited lesion expansion on apple in laboratory ***,Pseudomonas *** ZO-J could stably colonize on the apple peel for 12 *** results showed that Pseudomonas *** ZO-J can be further developed as an effective biocontrol agent for postharvest brown rot of apple.

关键词： Postharvest diseases Colonization Microscopic observation Endophytic bacteria

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华中师范大学学报（自然科学版） 2010年第3期44卷 472-477页

作者：吴萍李正鹏安徽科技学院生命科学学院安徽凤阳233100

以豆秸粉为主要原料,通过单因素和正交试验确定金针菇(Flammulina velutipes)固态发酵生产木聚糖酶最佳培养条件,并对其粗酶的酶学性质进行了研究.结果表明,碳源为豆秸粉:玉米芯:麸皮=7:2:1,氮源为1.5%的黄豆粉,pH为5,接种量为干料的20%... 详细信息

以豆秸粉为主要原料,通过单因素和正交试验确定金针菇(Flammulina velutipes)固态发酵生产木聚糖酶最佳培养条件,并对其粗酶的酶学性质进行了研究.结果表明,碳源为豆秸粉:玉米芯:麸皮=7:2:1,氮源为1.5%的黄豆粉,pH为5,接种量为干料的20%,装瓶量为6 g/100 mL三角瓶装,料水比为1:2.1,培养天数5 d,在此培养条件下得到的酶活力最高,产量可达52 768.91 U/g.粗酶液的最适反应温度为60℃,最适反应pH为6.酶的pH稳定性及热稳定性较好,80℃时相对酶活力仍保持在40.41%.

关键词：木聚糖酶金针菇固态发酵正交试验

关中平原冬小麦临界氮稀释曲线和氮营养指数研究

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农业机械学报 2015年第10期46卷 177-183,273页

作者：李正鹏冯浩宋明丹西北农林科技大学水土保持研究所陕西杨凌712100 中国科学院水利部水土保持研究所陕西杨凌712100 西北农林科技大学中国旱区节水农业研究院陕西杨凌712100

在整理分析关中平原7年氮肥大田试验的基础上,分析比较了不同氮肥处理下冬小麦的氮浓度稀释曲线,并构建了关中平原主栽品种小偃22地上部生物量的临界氮浓度稀释曲线模型。结果表明：不施氮、氮肥不足、氮肥适宜和过量施氮4组处理的平均... 详细信息

在整理分析关中平原7年氮肥大田试验的基础上,分析比较了不同氮肥处理下冬小麦的氮浓度稀释曲线,并构建了关中平原主栽品种小偃22地上部生物量的临界氮浓度稀释曲线模型。结果表明：不施氮、氮肥不足、氮肥适宜和过量施氮4组处理的平均产量分别为4 263、5 097、6 023、6 203 kg/hm2,氮肥不足处理显著降低了小麦产量,过量施氮与氮肥适宜处理相比产量没有显著差异。植株氮浓度均随生物量增加而降低,较高的施氮量导致较高的植株氮浓度。关中平原冬小麦临界氮浓度与地上部最大生物量符合幂函数关系。利用氮营养指数NNI（植株实际测定的氮浓度与临界氮浓度的比值）和2010—2011年冬小麦季的独立试验资料对建立的临界氮稀释曲线进行检验,结果表明：不施氮、氮肥不足、氮肥适宜和过量施氮4组处理的NNI值分别为0.70、0.89、1.01和1.25;关中平原各试验地土壤肥力差异较大,冬小麦适宜施氮量为75～180 kg/hm2,平均为137 kg/hm2。该研究建立的临界氮稀释模型的准确性较高,所得出的分析结果可靠,可以用于诊断和调控冬小麦氮素营养。

关键词：冬小麦临界氮浓度氮营养诊断关中平原优化施氮

水氮耦合下冬小麦LAI与株高的动态特征及其与产量的关系

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农业工程学报 2017年第4期33卷 195-202页

作者：李正鹏宋明丹冯浩西北农林科技大学水土保持研究所杨凌712100 西北农林科技大学中国旱区节水农业研究院杨凌712100 中国科学院水利部水土保持研究所杨凌712100

为了进一步阐明灌水施氮对小麦生长(叶面积指数(leaf area index,LAI)和株高)和产量的影响机制,本研究在2012-2014年2a大田试验基础上,采用修正的Logistic和Richards数学模型定量分析了不同灌水施氮处理对LAI和株高的动态发育的影响,以... 详细信息

为了进一步阐明灌水施氮对小麦生长(叶面积指数(leaf area index,LAI)和株高)和产量的影响机制,本研究在2012-2014年2a大田试验基础上,采用修正的Logistic和Richards数学模型定量分析了不同灌水施氮处理对LAI和株高的动态发育的影响,以及LAI和株高动态变化过程与产量及产量构成的回归关系。结果表明灌水能显著提高LAI的最大扩展速率(LRmax),从而增大最大LAI和平均LAI。施氮通过降低达到最大速率和最大LAI时的积温、增大LRmax来提高最大LAI和平均LAI(分别由不施氮下的1.87、1.35 cm^2/cm^2增大到施氮210 kg/hm^2条件下的4.57、3.82 cm^2/cm^2,继续施氮,增加效果不显著)。灌水通过延长株高生长时间来增大株高。施氮通过缩短株高进入快速生长期的时间和延长株高生长时间来增大株高,最大株高由不施氮下的58 cm提高到施氮105 kg/hm^2条件下的65 cm,继续施氮对株高增加不显著。年份对株高的生长影响显著。产量及产量构成与株高、LAI过程的逐步回归分析表明产量由平均LAI和最大株高共同决定,平均LAI主要决定了每平方米穗数,而最大株高主要决定了千粒质量,平均LAI对产量贡献更多。该研究为揭示水肥对作物生长过程和产量形成提供理论依据,为合理调控作物群体结构提供技术支撑。

关键词：灌溉氮作物 Logistic方程 Richards方程产量构成要素