检索结果-南通市图书馆

知母特异性DNA条形码筛选、种质资源鉴定与遗传多样性分析

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

中国中药杂志 2024年第1期49卷 88-99页

作者：窦西铭张靖晗王馨刘珊瑚石玥黄钰莹张晓芹何高洁安克露戚源王晓晖魏胜利张媛北京中医药大学中药学院北京102488 北京中医药大学北京中医药研究院北京102488 北京中医药大学生命科学院北京102488 河北序彤种业集团有限公司河北石家庄050501

知母Anemarrhena asphodeloides是临床方剂和中成药中常用药材。该研究利用Illumina平台测序获得7份不同产地的知母叶绿体基因组序列,通过比较基因组学分析筛选特异性DNA条形码,并利用DNA条形码对全国不同产地知母样品进行种质资源鉴定... 详细信息

知母Anemarrhena asphodeloides是临床方剂和中成药中常用药材。该研究利用Illumina平台测序获得7份不同产地的知母叶绿体基因组序列,通过比较基因组学分析筛选特异性DNA条形码,并利用DNA条形码对全国不同产地知母样品进行种质资源鉴定与遗传多样性分析。7份知母叶绿体基因组均具有环状结构,全长为156801~156930 bp,均注释113个基因,其中蛋白编码基因79个、tRNA基因30个、rRNA基因4个;比较基因组学分析显示rps16、trnG-GCC、atpF、rpoB、ycf3、rpl16、ndhF、trnS-GCU_trnG-GCC、petN-psbM、ndhF-rpl32可作为潜在的知母特异性DNA条形码。该研究选取序列长度在700~800 bp,且易于扩增的ycf3(第2内含子区)和atpF(内含子区)序列对26个产地594份知母样品进行PCR扩增和测序,序列分析结果显示ycf3和atpF序列分别能鉴定6、8个单倍型,2个序列联合分析能鉴别17个单倍型,命名为Hap1~Hap17。26个知母居群单倍型多样性(Hd)为0.68;核苷酸多样性(Pi)为0.93×10^(-3);遗传距离为0~0.0031,表明知母种内遗传多样性较丰富。中介邻接网络分析显示Hap5为最古老单倍型,主要分布在河北保定易县、山西忻州河曲县和山西临汾襄汾县。该研究对知母品种的鉴定、种质资源保护和开发及产地鉴别具有重要指导意义,为进一步揭示知母的遗传进化规律提供了研究基础。

关键词：知母叶绿体基因组种质资源单倍型遗传多样性

植物精油对西门塔尔公牛血清抗氧化性能和直肠微生物的影响

在线全文

学校读者我要写书评

暂无评论

饲料研究 2024年第11期47卷 1-6页

作者：王晓林吴春会肖阳李伟刘春海张秀江王明亚李秋凤河北农业大学动物科技学院河北保定071000 定兴县动物疫病预防控制中心河北保定071000 黑龙江省农业科学院畜牧兽医分院黑龙江齐齐哈尔161005 辽宁菲迪饲料科技有限责任公司辽宁兴城125100 保定市农业局河北保定071000

试验旨在研究植物精油(PEO)对西门塔尔公牛血清抗氧化性能和直肠微生物的影响。选择56头体重(564.39±7.09)kg、体况良好的西门塔尔公牛,随机分成4组,每组14个重复,每个重复1头牛。对照组(CK)饲喂基础饲粮,处理组在基础饲粮中分别添加0.... 详细信息

试验旨在研究植物精油(PEO)对西门塔尔公牛血清抗氧化性能和直肠微生物的影响。选择56头体重(564.39±7.09)kg、体况良好的西门塔尔公牛,随机分成4组,每组14个重复,每个重复1头牛。对照组(CK)饲喂基础饲粮,处理组在基础饲粮中分别添加0.5(PEO1)、1.0(PEO2)、1.5(PEO3)g/(头·d)的PEO。预试期7 d,正式试验期60 d。结果表明:试验第30 d,PEO2组血清总抗氧化能力(T-AOC)显著高于CK组(Pda)含量显著低于CK组(P0.05)。在直肠微生物门水平上,PEO对各菌门的相对丰度无显著影响(P>0.05)。在直肠微生物科水平上,PEO1组韦荣氏菌科(Veillonellaceae)相对丰度显著低于CK组(P<0.05)。PEO2组克里斯滕森菌科(Christensenellaceae)相对丰度显著高于CK组、PEO1组和PEO3组(P<0.05)。在直肠微生物属水平上,PEO3组普雷沃氏菌属(Prevotella)相对丰度显著高于CK组和PEO2组(P<0.05)。PEO2组丁酸弧菌(Butyrivibrio)相对丰度显著高于CK组、PEO1组和PEO3组(P<0.05)。研究表明,饲粮中添加PEO可以提高西门塔尔公牛血清抗氧化性能,改善肠道微生物菌群结构,以1.0 g/(头·d)为适宜添加量。

关键词：植物精油西门塔尔公牛抗氧化性能直肠微生物

维普期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

深圳市新生儿脑卒中多中心现况调查

中国当代儿科杂志 2024年第5期26卷 450-455页

作者：时莉秀冯晋兴韦艳芳路欣茹张雨茜杨林颖何胜男陈佩娟韩静陈诚涂惠英余章斌黄进洁曾淑娟陈晚铃刘颖郭燕平毛姣玉李晓东张谦慎谢治利黄梅英晏昆山应尔雅陈俊王艳荣刘亚萍宋波刘华艳肖晓冬唐红王宇娜蔡茵莎龙琦许汉强王会战孙倩韩芳张瑞彪杨传忠窦蕾时会菊王瑞蒋萍无深圳市儿童医院新生儿科广东深圳518034 深圳市宝安区妇幼保健院新生儿科广东深圳518101 深圳市龙岗区妇幼保健院/汕头大学医学院龙岗妇幼临床学院新生儿科广东深圳518172 深圳市人民医院/暨南大学第二临床医学院/南方科技大学第一附属医院新生儿科广东深圳518020 深圳市龙岗中心医院/广州中医药大学深圳临床医学院新生儿科广东深圳518116 北京大学深圳医院新生儿科广东深圳518036 华中科技大学协和深圳医院新生儿科广东深圳518052 香港大学深圳医院新生儿科广东深圳518053 深圳市龙岗区人民医院新生儿科广东深圳518172 深圳市南山区妇幼保健院新生儿科广东深圳518067 深圳市第三人民医院新生儿科广东深圳518112 深圳市龙华区中心医院新生儿科广东深圳518110 深圳市盐田区人民医院新生儿科广东深圳518081 深圳远东妇产医院新生儿科广东深圳518002 深圳市第二人民医院新生儿科广东深圳518025 陆丰市人民医院新生儿科广东陆丰516500 惠阳三和医院新生儿科广东惠州518200 惠州市第三人民医院新生儿科广东惠州516100 深圳市妇幼保健院新生儿科广东深圳518028 南方科技大学医院新生儿科广东深圳518055 深圳市罗湖区人民医院新生儿科广东深圳518005 深圳新生儿数据协作网

目的总结深圳市新生儿脑卒中发病率、临床特征及预后等情况,提高脑卒中新生儿的早期诊治能力。方法由深圳市儿童医院牵头,收集2020年1月—2022年12月深圳新生儿数据协作网组织21家单位收治的新生儿脑卒中36例,分析深圳市新生儿脑卒中发... 详细信息

目的总结深圳市新生儿脑卒中发病率、临床特征及预后等情况,提高脑卒中新生儿的早期诊治能力。方法由深圳市儿童医院牵头,收集2020年1月—2022年12月深圳新生儿数据协作网组织21家单位收治的新生儿脑卒中36例,分析深圳市新生儿脑卒中发病率、临床特征、治疗、预后等情况。结果深圳市21家医院2020、2021、2022年新生儿脑卒中的发病率分别为1/15137、1/6060、1/7704。缺血性脑卒中占75%(27/36),男性患儿占64%(23/36)。86%(31/36)的患儿在生后3d内起病,53%(19/36)以惊厥起病。颅脑影像学结果显示,22例(61%)大脑左侧发生梗死,13例(36%)基底节梗死;12例完成磁共振血管成像检查,9例(75%)累及大脑中动脉。29例患儿完成脑电图检查,21例(72%)出现尖波,10例(34%)出现电发作。不同医院对症支持治疗不同。12例(33%)完善新生儿行为神经测定评分,平均得分(32±4)分。共随访27例患儿12月龄左右的预后情况,44%(12/27)的患儿预后良好。结论新生儿脑卒中主要为缺血性脑卒中,多以惊厥为首发症状,多累及大脑中动脉,脑电图多出现尖波,神经发育评分较低,以对症支持治疗为主,部分患儿预后不良。

关键词：脑卒中多中心研究新生儿

Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2016年第1期15卷 120-134页

作者： li Yu wang Ke XIE hui XU Chun-ling wang dong-wei li Jing huang Xin PENG xiao-fang Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine Department of Plant Pathology South China Agricultural University

Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach （Ipomoea aquatic）, malabar spinach （Basella rubra）, and squash （Cucurbita moschata） in pots. The re- sults showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathoge- nicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.

关键词： burrowing nematode parasitism pathogenicity genetic diversity PCR-RFLP

Proteomic Analysis of Wheat Seed in Response to Drought Stress

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第5期13卷 919-925页

作者： ZHANG Yu-feng huang xiu-wen wang li-li wei liu WU zhi-hui YOU Ming-shan li Bao-yun Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization Ministry of Education/College of Agronomy and Biotechnology China Agricultural University

Drought stress is one of the major factors affecting in wheat yield and grain quality. In order to investigate how drought stress might influence wheat quality during grain filling, three wheat cultivars Gaocheng 8901, Jagger and Nongda 3406 were subjected to drought stress during the grain filling stage. Neither globulin and glutenin, nor the relative percentage ofamylose significantly changed following drought treatments, whereas albumin and gliadin concentrations did. The SDS-sedimentation, which has a strong linear correlation with wheat baking quality was markedly decreased following drought stress. These results indicated that drought had an adverse effect on wheat qualit3,. In order to investigate the protein complexes in the wheat flour, the data from native PAGE and SDS-PAGE were combined and a total of 14 spots were successfully identified, and of these eight protein types were determined to be potential complex forming proteins.

关键词： wheat seed drought stress two-dimensional electrophoresis (2-DE)

变暖背景下砾石换填对多年冻土区机场跑道下地温场的影响

在线全文

学校读者我要写书评

暂无评论

气候变化研究进展 2024年第3期20卷 291-303页

作者：王娇娇张虎金晓颖黄帅王宏伟王文辉詹涛周刚义车富强李艳李新宇何瑞霞张泽张圣嵘李国玉童长江王逊金会军东北林业大学土木与交通学院冻土研究所/教育部东北冻土地质系统与环境观测试验研究站哈尔滨150040 黑龙江省自然资源调查院大兴安岭漠河冻土与寒区环境野外科学观测研究站(自然资源部) 哈尔滨150036 中国科学院西北生态环境资源研究院甘肃遥感重点实验室和黑河遥感试验研究站兰州730000 国家林业和草原局重点国有林区森林资源监测中心加格达奇165100 中国科学院西北生态环境资源研究院冻土工程国家重点实验室兰州730000 哈尔滨工业大学土木工程学院哈尔滨150090

受气候变暖影响,东北北部地区冻融灾害频发,对寒区工程设施造成了重要影响。地基温度场的研究是分析与解决工程基础冻融灾害的重要手段。文中我们以漠河机场跑道为对象,通过有限单元法研究了洁净砾石换填对机场道基温度场的影响,并对运... 详细信息

受气候变暖影响,东北北部地区冻融灾害频发,对寒区工程设施造成了重要影响。地基温度场的研究是分析与解决工程基础冻融灾害的重要手段。文中我们以漠河机场跑道为对象,通过有限单元法研究了洁净砾石换填对机场道基温度场的影响,并对运行30年内道基温度场进行预测。结果表明,换填使季节最大冻结深度(MSF)增加,且换填对道基下MSF的水平影响范围在道面中心线两侧30 m左右。之后,通过比较不同换填深度(1.5(顶)~3.5(底)、1.5~4.5、1.5~5.5和1.5~6.5 m)的道基温度场变化,发现:换填底部深度达到4.5 m时,MSF变化的速率开始减小。最后,根据IPCC第六次评估报告(AR6)未来100年间不同气候变暖速率模型模拟研究了无换填与不同换填深度下的MSF可能变化。结果发现,到2100年,在SSP2-4.5情景下,未换填及不同换填深度的道基下MSF分别为1.63、1.86、1.84、1.84和1.84 m。因此,利用换填法来减少跑道冻融灾害时换填深度应至少达到4.5 m。同时,应加强漠河机场道基地表水与该跑道区地下水的防排水设施建设与维运。研究结果有助于进一步认识换填对多年冻土和活动层水热状态的影响,可为解决道基冻胀和融沉问题提供重要科学依据。

关键词：多年冻土机场跑道地温季节最大冻结深度(MSF) 数值模拟

CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumorassociated macrophage polarization via FBXW7-N0TCHCCL2/ CSF1 axis

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第2期6卷 482-495页

作者： Yao-hui huang Kun Cai Peng-Peng Xu li wang Chuan-Xin huang ying Fang Shu Cheng xiao Jian Sun Feng liu Jin-Yan huang meng-meng Ji wei-li Zhao Shanghai Istitute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRulin Hospital Afliated to Shanghai Jiao Tong University School of MedicineShanghaiChina School of life Sciences and Biotechnology.Shanghai Jiao Tong Universiy ShanghalChina Department of Immunobiology and Microbiology Shanghai Istitute of ImmunologyShanghai Jiao Tong University School of MedicineShanghaiChina Pole de Recherches Sino Francais en Science du Vivant et Genomique Laboratory of Molecular PathologyShanghalChina

Epigenetic alterations play an important role in tumor progression of diffuse large B-cell lymphoma(DL BCL).However,the biological relevance of epigenetic gene mutations on tumor microenvironment remains to be *** core set of genes relating to histone methylation(KMT2D,KMT2C,EZH2),histone acetylation(CREBBP,EP300),DNA methylation(TET2),and chromatin remodeling(ARID1A)were detected in the training cohort of 316 patients by whole-genome/exome sequencing(WGS/WES)and in the validation cohort of 303 patients with newly diagnosed DL BCL by targeted *** correlation with peripheral blood immune cells and clinical outcomes were *** mechanisms on tumor microenvironment were investigated both in vitro and in *** all 619 DLBCL patients,somatic mutations in KMT2D(19.5%)were most frequently observed,followed by mutations in ARID1A(8.7%),CREBBP(8.4%),KMT2C(8.2%),TET2(7.8%),EP300(6.8%),and EZH2(2.9%).Among them,CREBBP/EP300 mutations were signifcantly associated with decreased peripheral blood absolute lymphocyte-to-monocyte ratios,as well as inferior progression-free and overall *** B-lymphoma cells,the mutation or knockdown of CREBBP or EP300 inhibited H3K27 acetylation,downregulated FBXW7 expression,activated the NOTCH pathway,and downstream CCL2/CSF1 expression,resulting in tumor-associated macrophage polarization to M2 phenotype and tumor cell *** B-lymphoma murine models,xenografted tumors bearing CREBBP/EP300 mutation presented lower H3K27 acetylation,higher M2 macrophage recruitment,and more rapid tumor growth than those with CREBBP/EP300 wild-type control via FBXW7-NOTCH-CCL2/CSF1 *** work thus contributed to the understanding of aberrant histone acetylation regulation on tumor microenvironment as an alternative mechanism of tumor progression in DLBCL.

关键词： FBXW7 P300 macrophage

Neoadjuvant chemotherapy for locally advanced cervical cancer reduces surgical risks and lymph-vascular space involvement

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer 2011年第9期30卷 645-654页

作者： Yue wang Guang wang li-hui wei ling-hui huang Jian-liu wang shi-Jun wang xiao-Ping li dan-Hua Shen dong-Mei Bao Jian Gao Department of Obstetrics ＆ Gynecology People＇s Hospital Peking University Beijing 100044 P. R. China Department of Obstetrics ＆ Gynecology Hangu Hospital Tianjin 300480 P. R. China Department of Obstetrics ＆ Gynecology Jinghong Municipal Hospital Jinghong Yunnan 666100 P. R. China Department of Pathology People＇s Hospital Peking University Beijing 100044 P. R. China Department of Radiology People＇s Hospital Peking University Beijing 100044. p. R. China.

Neoadjuvant chemotherapy(NACT),which can reduce the size and therefore increase the resectability of tumors,has recently evolved as a treatment for locally advanced cervical *** has been reported to decrease the risk of pathologic factors related to prognosis of cervical *** further assess the effects of NACT on surgery and the pathologic characteristics of cervical cancer,we reviewed 110 cases of locally advanced cervical cancer treated with radical hysterectomy with or without NACT at the People's Hospital of Peking University between January 2006 and December *** 110 patients,68 underwent platinum-based NACT prior to surgery(NACT group) and 42 underwent primary surgery treatment(PST group).Our results showed 48 of 68(70.6%) patients achieved a complete response or partial response to *** blood loss,operation time,and number of removed lymph nodes during surgery,as well as complication rates during and after surgery were not significantly different between the NACT group and the PST *** rates of deep stromal invasion,positive parametria,positive surgical vaginal margins,and lymph node metastasis were not significantly different between the two ***,the rate of lymph-vascular space involvement(LVSI) was significantly lower in the NACT group than in the PST group(P = 0.021).In addition,the response rate of NACT was significantly higher in the patients with chemotherapeutic drugs administrated via artery than via *** results suggest that NACT is a safe and effective treatment for locally advanced cervical cancer and significantly decreases the rate of LVSI.

关键词：手术治疗子宫颈癌化疗药物淋巴结晚期间隙血管风险

Advances in Climate Change Research 12(2021)48-65 Review

在线全文

学校读者我要写书评

暂无评论

Advances in Climate Change Research 2021年第1期12卷 48-65页

作者： li xiao-ying JIN hui-Jun wang Hong-wei Sergey SMARCHENKO SHAN wei LUO dong-liang HE Rui-Xia Valentin SPEKTOR huang Ya-dong li Xin-Yu JIA Ning Key Laboratory of Sustainable Forest Ecosystem Management(Ministry of Education) College of ForestryNortheast Forestry UniversityHarbin150040China Northeast-China Observatory and Research Station of Permafrost Geological Environment(Ministry of Education) School of Civil EngineeringInstitute of Cold Region Science and EngineeringNortheast Forestry UniversityHarbin150040China State Key Laboratory of Frozen Soils Engineering Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou730000China Permafrost Lab Geophysical InstituteUniversity of Alaska FairbanksFairbanks99775USA Melnikov Permafrost Institute Siberian BranchRussian Academy of SciencesYakutsk677010Russia School of Civil Engineering Harbin Institute of TechnologyHarbin150090China

In boreal and arctic regions,forest fires exert great influences on biogeochemical processes,hydrothermal dynamics of the active layer and near-surface permafrost,and subsequent nutrient *** this article,the studies on impacts of forest fires on the permafrost environment are *** studies indicate that forest fires could result in an irreversible degradation of permafrost,successions of boreal forests,rapid losses of soil carbon stock,and increased hazardous periglacial *** forest fires,soil temperatures rise;active layer thickens;the release of soil carbon and nitrogen enhances,and;vegetation changes from coniferous forests to broad-leaved forests,shrublands or *** may take decades or even centuries for the fire-disturbed ecosystems and permafrost environment to return to pre-fire conditions,if ever *** boreal forest,the thickness of organic layer has a key influence on changes in permafrost and *** addition,climate warming,change of vegetation,shortening of fire return intervals,and extent of fire range and increasing of fire severity may all modify the change trajectory of the fire-impacted permafrost ***,the observations and research on the relationships and interactive mechanisms among the forest fires,vegetation,carbon cycle and permafrost under a changing climate are still inadequate for a systematic impact *** the chronosequence approach of evaluating the temporal changes by measuring changes in the permafrost environment at different stages at various sites(possibly representing varied stages of permafrost degradation and modes),multi-source data assimilation and model predictions and simulations should be integrated with the results from long-and short-term field investigations,geophysical investigations and airborne surveys,laboratory testing and remote *** studies may enable quantitatively assess and predict the feed-back relationship and influence mechanism a

关键词： Forest fires Hydrothermal processes Organic layer thickness Active layer thickness Ecological thresholds Permafrost degradation Boreal and arctic regions