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Virus Location and Lymphocyte Apoptosis in Lymph Nodes of Piglets Infected with PCV-2

Agricultural Sciences in China 2008年第4期7卷 507-512页

作者： SHI Lū-juan HAN Hui-li ZHANG shu-xia College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 P.R.China

The present study has been performed to understand the location of the virus, type of apoptotic cells, and their relation to lymph nodes of piglets infected with porcine circovirus type Ⅱ （PCV-2）. Nine 32-day-old conventional piglets free of infection with PCV-2 were used, and distributed into three groups： control group （n = 3）, piglets inoculated with PCV-2 alone （PCV-2, n = 3）, and PCV-2 inoculated and KLH immunostimulated group （PCV-2 ＋ KLH, n = 3）. Superficial inguinal lymph nodes from all piglets were collected for histological examination after 32 days postinoculation, and immunohistochemistry for PCV-2 detection. Location of apoptotic cells was detected with TdT-mediated dUTP nick end labeling （TUNEL） and cell cycle, and the apoptotic rates were measured by flow cytometry. The characteristic histopathological lesions of the piglets in PCV-2 and PCV-2 ＋ KLH were lymphocyte depletions in the cortex and paracortex of the lymph nodes, epithelioid-like macrophage infiltration, and intracytoplasmic inclusion bodies presented in epithelioid-like macrophages. PCV-2 was mainly found in epithelioid-like macrophages by immunohistochemistry. In the lymph nodes, lymphocytes presented higher apoptotic rates in the cortex by TUNEL, special B-cell areas, and similar apoptotic cells were found in this compartment in the control. The apoptotic rates of the lymph nodes were 0.41, 3.34, and 4.88% in the control, PCV-2, and PCV-2 ＋ KLH groups by flow cytometry, respectively. The apoptotic rates of lymph nodes for PCV-2 and PCV-2 ＋ KLH piglets were significantly higher than those for the control group （P〈0.05 and P〈0.01）. The proliferation index （PI） was 0.17_＋0.01, 0.12_＋0.01 and 0.12_＋0.04 in the control, PCV-2, and PCV-2 ＋ KLH group, the PI of the control group was higher than that of the other groups, but without the statistical difference. PCV-2 can induce lymphocyte depletion in lymph nodes of piglets by blocking cell proliferation and promoting apoptosis. This is one of th

关键词： apoptosis virus located lymph node PCV-2 piglets

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Variable hydrological effects of herbs and shrubs in the arid northeastern Qinghai-Tibet Plateau,China

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Journal of Mountain Science 2018年第7期15卷 1532-1545页

作者： liU Ya-bin ZHANG Ying FU Jiang-tao YU Dong-mei HU xia-song li Xi-lai QI Zhao-xin li shu-xia Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources Qinghai Institute of SaltLakes Chinese Academy of Sciences Xining 810008 China Qinghai Provincial Key Laboratory of Geology and Environment of Salt Lakes Xining 810008 China University of Chinese Academy of Sciences Beijing 100049 China Department of Geological Engineering Qinghai University Xining 810016 China College of Agriculture and Animal Husbandry Qinghai University Xining 810016 China

This study aims to assess the hydrological effects of four herbs and four shrubs planted in a selfestablished test area in Xining Basin of northeastern Qinghai-Tibet Plateau, China. The Rainfallintercepting Capability(RIC) of the herbs and shrubs was evaluated in rainfall interception experiment at the end of the third, fourth and fifth month of the growth period in 2007. The leaf transpiration rate and the effects of roots on promoting soil moisture evaporation in these plants were also assessed in transpiration experiment and root-soil composite system evaporation experiment in the five month's growth period. It is found that the RIC of the fourstudied herbs follows the order of E. repens, E. dahuricus, A. trachycaulum and L. secalinus; the RIC of the four shrubs follows the order of A. canescens, Z. xanthoxylon, C. korshinskii and N. tangutorum. The RIC of all the herbs is related linearly to their mean height and canopy area(R^2 ≥ 0.9160). The RIC of all the shrubs bears a logarithmic relationship with their mean height(R^2 ≥ 0.9164), but a linear one with their canopy area(R^2 ≥ 0.9356). Moreover, different species show different transpiration rates. Of the four herbs, E. repens has the highest transpiration rate of 1.07 mg/(m^2·s), and of the four shrubs, A. canescens has the highest transpiration rate(0.74 mg/(m^2·s)). The roots of all the herbs and shrubs can promote soil moisture evaporation. Of the four herbs, the evaporation rate of E. repens root-soil composite system is the highest(2.14%), and of the four shrubs,the root-soil composite system of A. canescens has the highest evaporation rate(1.41%). The evaporation rate of the root-soil composite system of E. dahuricus and Z. xanthoxylon bears a second-power linear relationship with evaporation time(R^2 ≥ 0.9924). The moisture content of all the eight root-soil composite systems decreases exponentially with evaporation time(R^2 ≥ 0.8434). The evaporation rate and moisture content of all the plants' root-

关键词： Plant hydrological effects Rainfall interception capacity Root-soft composite system Transpiration rate Moisture evaporation rate Qinghai-Tibet Plateau

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Influences of soil moisture and salt content on loess shear strength in the Xining Basin, northeastern Qinghai-Tibet Plateau

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Journal of Mountain Science 2019年第5期16卷 1184-1197页

作者： FU Jiang-tao HU xia-song li Xi-lai YU Dong-mei liU Ya-bin YANG You-Qing QI Zhao-xin li shu-xia Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources Qinghai Institute of Salt Lakes Chinese Academy of Sciences Qinghai Provincial Key Laboratory of Geology and Environment of Salt Lakes University of Chinese Academy of Sciences Department of Geological Engineering Qinghai University College of Agriculture and Animal Husbandry Qinghai University

Moisture and salt content of soil are the two predominant factors influencing its shear strength. This study aims to investigate the effects of these two factors on shear strength behavior of loess in the Xining Basin of Northeast Qinghai-Tibet Plateau, where such geological hazards as soil erosion, landslides collapse and debris flows are widespread due to the highly erodible loess. Salinized loess soil collected from the test site was desalinized through salt-leaching in the laboratory. The desalinized and oven-dried loess samples were also artificially moisturized and salinized in order to examine how soil salinity affects its shear strength at different moisture levels. Soil samples prepared in different ways(moisturizing, salt-leaching, and salinized) were measured to determine soil cohesion and internal friction angle. The results show that salt-leaching up to 18 rounds almost completely removed the salt content and considerably changed the physical components of loess, but the soil type remained unchanged. As salt content increases from 0.00% to 12.00%, both the cohesion and internal friction angle exhibit an initial decrease and then increase with salt content. As moisture content is 12.00%, the salt content threshold value for both cohesion and internal friction angle is identified as 3.00%. As the moisture content rises to 16.0% and 20.00%, the salt content threshold value for cohesion is still 6.00%, but 3.00% for internal friction angle. At these thresholds soil shear strength is the lowest, below which it is inversely related to soil salinity. Beyond the thresholds, however, the relationship is positive. Dissimilar to salinity, soil moisture content exerts an adverse effect on shear strength of loess. The findings of this study can provide a valuable guidance on stabilizing the engineering properties of salinized loess to prevent slope failures during heavy rainfall events.

关键词： Salinized loess Desalinized loess Salt-leaching Shear strength indices Moisture content Salt content

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A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression

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Journal of Integrative Agriculture 2022年第9期21卷 2588-2602页

作者： DONG Shi-man xiaO liang li Zhi-bo SHEN Jie YAN Hua-bing li shu-xia liAO Wen-bin PENG Ming Key Laboratory of Biology and Genetic Resources of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101P.R.China Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101P.R.China Cash Crops Research Institute Guangxi Academy of Agricultural SciencesNanning 530007P.R.China College of Tropical Crops Hainan UniversityHaikou 570228P.R.China

Cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs(lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA(DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid(ABA) or jasmonic acid(JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein–protein interaction(PPI)analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control ***, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

关键词： lncRNA RNA-seq drought stress cassava DIR

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Design of Scheduling Decision Mechanism for Agile Manufacturing System

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Computer Aided Drafting,Design and Manufacturing 2007年第1期17卷 1-8页

作者： li shu-xia SHAN Hong-bo School of Business East China University of Science ＆ Technology Shanghai 200237 China College of Mechanical Engineering Donghua University Shanghai 200051 China

Design of scheduling decision mechanism is a key issue of scheduling decision method and strategy for agile manufacturing system. Effective scheduling decision mechanism helps to improve the operational agility of manufacturing system. Several scheduling decision mechanisms are discussed, including scheduling forecasting mechanism, cooperation mechanism and cell scheduling mechanism. Also soft decision mechanism is put forward as a promising prospect for agile manufacturing system, and some key techniques in soft decision mechanism are introduced.

关键词： agile manufacturing system scheduling decision mechanism soft decision

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Thermochemistry study on H2O/NaY zeolite adsorption system

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Chinese Journal of Chemistry 1988年第1期6卷 7-13页

作者： ZHOU li-Xing CHEN shu-xia ZHENG Lu-Bin Dalian Institute of Chemical Physics,Academia Sinica,Dalian Dalian Institute of Chemical Physics Academia Sinica Dalian

By using an automatic adiabatic calorimeter the heat capacity measurements in the tem-perature range of 220—320K for HO/NaY zeolite adsorption system with various amounts of adsorbedwater have been *** c-T curves obtained,there is no peak for solid-liquid phase transitionof the adsorbed *** for HO-NaY zeolite system which consists of a saturated HO/NaYadsorption system mixed mechanically with a certain amount of water,there are distinct peaks intheir *** peak in the c-Tcurve disappeared as soon as the mixed water in the lattersystem was *** facts mentioned above have been discussed from the point of view ofthe structure of the adsorbed layer and the pore size of zeolite.

关键词： NaY Thermochemistry study on H2O/NaY zeolite adsorption system

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注热水盐度对水合物开采影响的实验研究

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高校化学工程学报 2015年第2期29卷 482-486页

作者：李淑霞李杰曹文中国石油大学(华东)石油工程学院山东青岛266580 国土资源部天然气水合物重点实验室山东青岛266071 中海石油(中国)有限公司深圳分公司研究院广东广州510240

天然气水合物是目前备受关注的新型洁净优质能源,注热盐水是一种有效的开采方式。采用自制的一维天然气水合物(NGH)开采模拟实验装置,首先在填砂模型中生成初始条件相同的天然气水合物藏,之后注入盐度为2%、10%、20%的热水进行开采实验... 详细信息

天然气水合物是目前备受关注的新型洁净优质能源,注热盐水是一种有效的开采方式。采用自制的一维天然气水合物(NGH)开采模拟实验装置,首先在填砂模型中生成初始条件相同的天然气水合物藏,之后注入盐度为2%、10%、20%的热水进行开采实验。结果表明:当注热水盐度较大(达到10%后)时,水合物快速分解,与自由气同时产出,产气速率峰值较大;而当注热水盐度较小(2%)时,自由气首先产出,然后水合物分解气体产出。注热水盐度越大,水合物分解速度越快,热前缘移动速率越大,水合物大量分解阶段的能量效率越高。因此,注热水盐度的增加可以加快天然气水合物的开采速率,增大注热开采的经济可行性。

关键词：天然气水合物注热盐水盐度热前缘能量效率

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井间示踪剂产出曲线自动拟合方法

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石油勘探与开发 2005年第5期32卷 121-124页

作者：冯其红李淑霞中国石油大学(华东)石油工程学院

以对流扩散理论为基础,利用流管法导出了井间示踪剂产出浓度的理论公式。传统井间示踪剂产出曲线的解释方法需要大量调整井间参数和注入参数来拟合产出浓度,解释精度低,针对这些缺陷,提出利用遗传算法实现自动拟合产出浓度的参数解释模... 详细信息

以对流扩散理论为基础,利用流管法导出了井间示踪剂产出浓度的理论公式。传统井间示踪剂产出曲线的解释方法需要大量调整井间参数和注入参数来拟合产出浓度,解释精度低,针对这些缺陷,提出利用遗传算法实现自动拟合产出浓度的参数解释模型,只需给出该模型中各参数变化的大体范围,无须人为调参,模型自动拟合产出曲线,求解参数的最优解。应用结果表明,该方法的收敛速度快、解释精度高。

关键词：示踪剂产出曲线自动拟合参数解释遗传算法

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碳税的效率与公平目标的协调性研究

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求是学刊 2012年第5期39卷 44-50页

作者：李淑霞哈尔滨工业大学人文学院黑龙江哈尔滨150001

碳税是当前公共财政领域极富争论的研究主题。最优碳税即"庇古税"诠释了控制二氧化碳排放的最优途径,即通过征收碳税纠正化石燃料的生产或使用产生的外部效应的市场失灵。庇古税是有效率的,但是在帕累托意义上并不考虑分配问题。找出影... 详细信息

碳税是当前公共财政领域极富争论的研究主题。最优碳税即"庇古税"诠释了控制二氧化碳排放的最优途径,即通过征收碳税纠正化石燃料的生产或使用产生的外部效应的市场失灵。庇古税是有效率的,但是在帕累托意义上并不考虑分配问题。找出影响碳税效率与公平之间协调的因素,以及给出实现碳税效率与公平协调的概念框架是当前亟须解决的重要问题。以碳税税率为主线,发现碳税的效率与公平目标之间缺乏协调性,并且指出影响这种协调的内生制度约束则是碳税的收入循环效应。

关键词：碳税经济效率公平协调性

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基于敏捷制造单元的车间动态重构

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计算机集成制造系统 2007年第3期13卷 520-526页

作者：李淑霞单鸿波华东理工大学商学院管理科学与工程系上海200237 东华大学机械工程学院上海200051

为适应现代车间的敏捷化需求,提出了一种基于敏捷制造单元的车间动态重构方法。车间的动态重构是一个优化制造资源,进而构造面向产品的敏捷制造单元,形成虚拟车间的过程,采用数学规划和模糊理论相结合的方法,优化制造资源。同时,提出了... 详细信息

为适应现代车间的敏捷化需求,提出了一种基于敏捷制造单元的车间动态重构方法。车间的动态重构是一个优化制造资源,进而构造面向产品的敏捷制造单元,形成虚拟车间的过程,采用数学规划和模糊理论相结合的方法,优化制造资源。同时,提出了一种基于不完全知识和进化博弈理论的动态单元重构模型,它能够面向不确定的需求目标,采用自下而上和自上而下相结合的方法,快速生成稳定优化的可重构制造单元,从而实现敏捷车间的组织再设计。该模型求解采用协同进化算法,并通过实例,验证了该方法的可行性和有效性。

关键词：敏捷制造单元动态重构进化博弈理论

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