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The AMPK and AKT/GSK3β pathways are involved in recombinant proteins fibroblast growth factor 1(rFGF1 and rFGF1a) improving glycolipid metabolism in rainbow trout(Oncorhynchus mykiss) fed a high carbohydrate diet

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Animal Nutrition 2024年第2期17卷 11-24页

作者： Huixia Yu Shuo Geng Shuai li Yingwei Wang Xin Ren Debin Zhong Haolin Mo mingxing Yao Jiajia Yu Yang li lixin Wang College of Animal Science and Technology Northwest A&F University Meixian Aquaculture Farm of Shitouhe Reservoir Administration

Fibroblast growth factor 1(FGF1) regulates vertebrate cell growth, proliferation and differentiation, and energy metabolism. In this study, we cloned rainbow trout(Oncorhynchus mykiss) fgf1 and fgf1a, prepared their recombinant proteins(rFGF1 and rFGF1a), and described the molecular mechanisms by which they improve glycolipid metabolism in carnivorous fish. A 31-d feeding trial was conducted to investigate whether they could enhance glycolipid metabolism in rainbow trout on high-carbohydrate diets(HCD). A total of 720 rainbow trout(8.9 ± 0.5 g) were equally divided into 4 groups: the chow diet(CD) group injected with PBS, the HCD group injected with PBS, the HCD group injected with rFGF1(400 ng/g body weight), and the HCD group injected with rFGF1a(400 ng/g body weight). The results showed that short-term HCD had a significant positive effect on the specific growth rate(SGR) of rainbow trout(P liver, serum TG, GOT, and GPT contents caused by HCD(P lied the promotion of TG catabolism. Moreover, rFGF1 and rFGF1a contributed to promoting lipolysis by activating the AMPK pathway and reducing lipid accumulation in the liver caused by HCD. In addition,the rFGF1 and r FGF1a-treated groups significantly reduced serum glucose levels and elevated hepatic glycogen content under HCD, and increased glucose uptake by hepatocytes. We observed a decrease in mRNA levels for pepck, g6pase, and pygl, along with an increase in mRNA levels for gys, glut2, and gk in the liver. Furthermore, these proteins regulated hepatic gluconeogenesis and glycogen synt

关键词： Rainbow trout Recombinant proteins Fibroblast growth factor 1 Glycogen synthesis lipid deposition

Conditional reprogramming:next generation cell culture

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Acta Pharmaceutica Sinica B 2020年第8期10卷 1360-1381页

作者： Xiaoxiao Wu Shengpeng Wang mingxing li Jing li Jing Shen Yueshui Zhao Jun Pang Qinglian Wen Meijuan Chen Bin Wei Parham Jabbarzadeh Kaboli Fukuan Du Qijie Zhao Chi Hin Cho Yitao Wang Zhangang Xiao Xu Wu Laboratory of Molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhou 646000China South Sichuan Institute of Translational Medicine Luzhou 646000China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacaoChina Department of Oncology and Hematology Hospital(T.C.M)Affiliated to Southwest Medical UniversityLuzhou 646000China Center of Radiation Oncology Hospital(T.C.M)Affiliated to Southwest Medical UniversityLuzhou 646000China Department of Oncology the Affiliated Hospital of Southwest Medical UniversitySouthwest Medical UniversityLuzhou 646000China College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals Zhejiang University of TechnologyHangzhou 310014China School of Biomedical Sciences Faculty of Medicinethe Chinese University of Hong KongHong KongChina

Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming(CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR cocultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase(ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years’ development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.

关键词： culture epithelium programming

A novel porous silica-zirconia coating for improving bond performance of dental zirconia

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第3期22卷 214-222页

作者： Zhiwei SU mingxing li ling ZHANG Chaoyang WANG Leiqing ZHANG Jingqiu XU Baiping FU The Affiliated Hospital of Stomatology School of StomatologyZhejiang University School of Medicineand Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhou 310006China

Objective:To coat a zirconia surface with silica-zirconia using a dip-coating technique and evaluate its effect on resin-zirconia shear bond strength(SBS).Methods:A silica-zirconia suspension was prepared and used to coat a zirconia surface using a dip-coating *** hundred and eighty-nine zirconia disks were divided into three groups according to their different surface treatments(polishing,sandblasting,and silica-zirconia coating).Scanning electron microscopy(SEM),energy dispersive X-ray(EDX),and X-ray diffraction(XRD)were used to analyze the differently treated zirconia *** primer treatments(Monobond N,Z-PRIME Plus,and no primer)were also applied to the zirconia ***,180 composite resin cylinders(Filtek Z350)were cemented onto the zirconia disks with resin cement(RelyX Ultimate).The SBS was measured after water storage for 24 h or 6 *** data were analyzed by two-way analysis of variance(ANOVA).Results:SEM and EDX showed that the silica-zirconia coating produced a porous layer with additional Si,and XRD showed that only tetragonal zirconia was on the silica-zirconia-coating *** with the control group,the resin-zirconia SBSs of the,andblasting group and silica-zirconia-coating group were significantly increased(Plica-zirconia coating followed by the application of Monobond N produced the highest SBS(Plica-zirconia might be a feasible way to improve resin-zirconia bonding.

关键词： Silica-zirconia coating Zirconia Bond performance Shear bond strength(SBS)

Small molecule inhibitors of RORγt for Th17 regulation in inflammatory and autoimmune diseases

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Journal of Pharmaceutical Analysis 2023年第6期13卷 545-562页

作者： Jiuping Zeng mingxing li Qianyun Zhao Meijuan Chen Long Zhao Shulin Wei Huan Yang Yueshui Zhao Anqi Wang Jing Shen Fukuan Du Yu Chen Shuai Deng Fang Wang Zhuo Zhang Zhi li Tiangang Wang Shengpeng Wang Zhangang Xiao Xu Wu Laboratory of Molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhouSichuan646000China Cell Therapy&Cell Drugs of Luzhou Key Laboratory LuzhouSichuan646000China South Sichuan Institute of Translational Medicine LuzhouSichuan646000China Department of Spleen and Stomach Diseases The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical UniversityLuzhouSichuan646000China School of Medicine Chengdu UniversityChengdu610106China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao999078China Department of Oncology The Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan646000China

As a ligand-dependent transcription factor,retinoid-associated orphan receptor gt(RORγt)that controls T helper(Th)17 cell differentiation and interleukin(IL)-17 expression plays a critical role in the progression of several inflammatory and autoimmune *** emerging novel approach to the therapy of these diseases thus involves controlling the transcriptional capacity of RORγt to decrease Th17 cell development and IL-17 *** RORγt inhibitors including both antagonists and inverse agonists have been discovered to regulate the transcriptional activity of RORγt by binding to orthosteric-or allosteric-binding sites in the ligand-binding *** of small-molecule inhibitors have entered clinical ***,in current review,the role of RORγt in Th17 regulation and Th17-related inflammatory and autoimmune diseases was ***,the recently developed RORγt inhibitors were summarized,with an emphasis on their optimization from lead compounds,efficacy,toxicity,mechanisms of action,and clinical *** limitations of current development in this area were also discussed to facilitate future research.

关键词： T helper 17 RORγt Small-molecule inhibitor Inflammatory disease Autoimmune disease

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超媒体与决策支持系统集成化的研究

计算机工程 1998年第7期24卷 20-22页

作者：刘翔李明星哈尔滨工业大学管理学院哈尔滨150001

提出了超媒体与决策支持系统（DSS）集成化的方法，给出了超媒体协同器功能结构设计方案，并就超媒体协同器关键技术的实现进行了研究。

关键词：超媒体决策支持系统多媒体集成化

矿井瞬变电磁PSO-DLS组合算法反演研究

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煤炭科学技术 2019年第9期47卷 268-272页

作者：李明星煤炭科学研究总院中煤科工集团西安研究院有限公司

隧道及煤矿井下巷道施工过程中面临严重的水文地质灾害问题,在复杂的施工环境及水文地质条件下,对水文地质的精确探测是十分困难的。矿井瞬变电磁法作为一种应用广泛的电磁感应勘探方法,对低阻体非常敏感,能够对隧道及巷道顶底板含水层... 详细信息

隧道及煤矿井下巷道施工过程中面临严重的水文地质灾害问题,在复杂的施工环境及水文地质条件下,对水文地质的精确探测是十分困难的。矿井瞬变电磁法作为一种应用广泛的电磁感应勘探方法,对低阻体非常敏感,能够对隧道及巷道顶底板含水层进行探测。为解决巷道或隧道瞬变电磁探测数据处理难度大且准确度不高的问题,通过对粒子群优化法(PSO)和阻尼最小二乘法(DLS)2种算法进行了比较分析研究,提出一种PSO-DLS组合反演算法,解决了PSO算法晚期寻优效率低下和DLS算法需要人工给定初始值的问题。结合某矿井实际地层资料,建立数学模型,分别利用PSO、DLS以及笔者提出的组合算法进行反演试算。研究结果表明:相比于两种单独的算法,组合算法具有更高的寻优效率且不需要人工给定初始模型,最后应用该组合算法对中国淮北煤田煤矿巷道实测瞬变电磁数据进行反演计算,结果实现了顶底板异常分离,解释成果与测井资料、地质资料及巷道揭露情况吻合,证明该组合算法能够对巷道瞬变电磁探测数据进行反演处理且准确度较高。

关键词： PSO算法 DLS算法组合算法矿井瞬变电磁

Flexible interdigitated layered framework with multiple accessible active sites for high-performance CH_(3)I capture

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Science China Chemistry 2024年第5期67卷 1569-1577页

作者： Bo Tai Baoyu li linwei He Zhonglin Ma Shujing lin mingxing Zhang Junchang Chen Fuqi Wu Long Chen Xing Dai Fuyin Ma Zhifang Chai Shuao Wang State Key Laboratory of Radiation Medicine and Protection School for Radiological and interdisciplinary Sciences(RAD-X)and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsSoochow UniversitySuzhou 215123China

The development of adsorbent materials for effective capture of radioactive iodomethane(CH_(3)I) from the off-gas of used nuclear fuel reprocessing, remains a significant and challenging line of research because currently state-of-art adsorbents still suffer from low binding affinity with CH_(3)I. Here, we proposed a brand-new adsorption topological structure by developing a 2D interdigitated layered framework, named SCU-20, featuring slide-like channel with multiple active sites for CH_(3)I capture. The responsive rotating-adaptive aperture of SCU-20 enables the optimal utilization of all active sites within the pore for highly selective recognition and capture of CH_(3)I. A record-breaking CH_(3)I uptake capacity of 1.84 g/g was achieved under static sorption conditions with saturated CH_(3)I vapor. Both experimental and theoretical findings demonstrated that the exceptional uptake of SCU-20 towards CH_(3)I can be attributed to the confined physical electrostatic adsorption of F sites, coupled with the chemical nitrogen methylation reaction with uncoordinated N atoms of pyrazine. Moreover, dynamic CH_(3)I uptake capacity potentially allows for the capture of CH_(3)I in simulated real-world off gas reprocessing conditions. This study highlights the potential of SCU-20 as a promising candidate for efficient capture of iodine species and contributes to the development of effective solutions for radioactive iodine remediation.

关键词： 2D interdigitated layered framework iodomethane dual-sites electrostatic interaction physisorption and chemisorption

Matrix-Mediated Color-Tunable Ultralong Organic Room Temperature Phosphorescence of 7H-Benzo[c]carbazole Derivatives

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CCS Chemistry 2024年第3期6卷 798-811页

作者： Chen Qian Xue Zhang Zhimin Ma Xiaohua Fu Zewei li Huiwen Jin mingxing Chen Hong Jiang Zhiyong Ma Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic–Inorganic CompositesCollege of Chemical EngineeringBeijing University of Chemical TechnologyBeijing 100029 Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871

Building on the recent systematic research on 1Hbenzo[f]indole(Bd),an important advancement in constructing ultralong organic room temperature(UORTP)materials with a universal strategy via a readily obtained unit(7H-Benzo[c]carbazole,BCz)is proposed in this *** powders of BCz and its derivatives merely exhibit blue fluorescence at ambient ***,when BCz and its derivatives are dispersed into polymer or powder matrixes,strong photo-activated green UORTP can be observed from their doped systems at room ***,the UORTP color can be tuned between green and yellow depending on the *** ultralong phosphorescence originates from the generation of charge-separated states via *** matrixes play a key role in both stabilizing charge-separated states and controlling UORTP *** interestingly,when using polymethyl methacrylate as matrix,the doped films achieve stronger photo-activated ultralong phosphorescence underwater than in air at room *** Bd,BCz achieves better performance not only in ultralong phosphorescence properties but also in practical *** work gains a deeper insight into the mechanism of UORTP and paves a new approach to applying organic phosphorescent materials to underwater coating and imaging.

关键词： 7H-Benzo[c]carbazole ultralong organic room temperature phosphorescence ion radical matrix-mediated color tunning underwater application

Oxidation of a Zr_(46)Cu_(46)Al_(7)Gd_(1)bulk metallic glass detected via optical characterizations

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Science China Materials 2021年第4期64卷 973-978页

作者： Weijie Xie mingxing li Jiashu Cao liwei Hu Chao Wang Wei-Hua Wang Yanhui liu Institute of Physics Chinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China

Oxidation of metallic glasses has been shown to be different from crystalline alloys due to the disordered atomic structures and far-from-equilibrium state,and quick characterization is required to reveal their early stage *** this study,we investigated the oxidation behavior of a Zr_(46)Cu_(46)Al_(7)Gd_(1)bulk metallic glass by measuring the changes of optical constants via spectroscopic *** with X-ray diffraction,scanning electron microscopy,and thermogravimetric analyzer confirmed that the changes of pseudo-optical constants reflected not only the onset temperature at which the alloys underwent obvious oxidation,but also the subtle oxidation which was tedious to *** study suggests a more efficient and accurate approach to understand the oxidation of metallic glasses with temperature,time and compositions.

关键词： metallic glasses spectroscopic ellipsometry oxidation