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激活鼠FcγRⅡb对天然免疫因子产生的影响

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中国生物工程杂志 2024年第5期44卷 10-19页

作者：赵睿杰孙杨杨李梦娟张晓晓张梦停张宜娜陈静夏平安河南农业大学动物医学院郑州450046

目的:了解FcγRIIb对小鼠巨噬细胞天然免疫及抗病毒免疫作用的影响。方法:构建鼠源FcγRIIb原核表达质粒pET28a-FcγRIIb,将其转化至大肠杆菌BL21感受态细胞中进行诱导表达,采用镍柱亲和层析法对重组蛋白进行纯化。以纯化后的重组蛋白... 详细信息

目的:了解FcγRIIb对小鼠巨噬细胞天然免疫及抗病毒免疫作用的影响。方法:构建鼠源FcγRIIb原核表达质粒pET28a-FcγRIIb,将其转化至大肠杆菌BL21感受态细胞中进行诱导表达,采用镍柱亲和层析法对重组蛋白进行纯化。以纯化后的重组蛋白作为抗原,免疫兔制备多克隆抗体,采用间接EliSA法检测血清效价,利用Western blotting技术鉴定抗体特异性,高免血清经饱和硫酸铵溶液粗纯和ProteinG Sapharose精纯获得兔抗鼠FcγRIIb多克隆抗体。以该抗体激活小鼠腹腔巨噬细胞表面的FcγRIIb,利用荧光定量PCR技术检测MHV感染上述细胞后相关细胞因子的mRNA转录水平。结果:单独激活FcγRIIb 12~48 h时巨噬细胞中TLR3、IRF3、IFN-α、IFN-β、IFN-γ、TNF-α和IL-1β的mRNA转录水平上调,免疫抑制因子TGF-β的mRNA转录水平下调。此外,在MHV感染小鼠腹腔巨噬细胞12~48 h时,与未激活组相比,FcγRIIb激活后可上调细胞中TLR3、IRF3、IFN-α、IFN-β、IFN-γ、TNF-α和IL-1β的mRNA转录水平,下调TGF-β的mRNA转录水平,且其病毒拷贝数及病毒滴度低于未激活组。结论:研究证明FcγRIIb的活化能促进小鼠腹腔巨噬细胞天然免疫因子产生,同时具有一定抗病毒作用,为进一步探究FcγRIIb在MHV感染过程中的作用奠定了基础。

关键词： FcγRⅡb 多克隆抗体小鼠肝炎病毒细胞因子天然免疫

眼轮匝肌“窗口法”在切开法重睑成形术中的应用

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中国美容整形外科杂志 2024年第9期35卷 519-521页

作者：王永洁李梦娟龙剑虹中南大学湘雅医院烧伤整形科湖南长沙410008

目的观察上睑提肌腱膜瓣或睑板前筋膜瓣穿过眼轮匝肌窗口前徙固定的仿生法重睑成形术的临床效果。方法自2018年7月至2020年1月,中南大学湘雅医院烧伤整形科对286例单睑患者采用眼轮匝肌“窗口法”行切开法重睑成形术。术中显露切口之间... 详细信息

目的观察上睑提肌腱膜瓣或睑板前筋膜瓣穿过眼轮匝肌窗口前徙固定的仿生法重睑成形术的临床效果。方法自2018年7月至2020年1月,中南大学湘雅医院烧伤整形科对286例单睑患者采用眼轮匝肌“窗口法”行切开法重睑成形术。术中显露切口之间眼轮匝肌,分别于内侧、中间、外侧去除约2 mm×2 mm的眼轮匝肌组织形成窗口,窗口内为上睑提肌腱膜组织或睑板前筋膜组织,形成上睑提肌腱膜瓣或睑板前筋膜瓣,分别将3处窗口部位的下唇皮肤、下唇眼轮匝肌、窗口内上睑提肌腱膜瓣或睑板前筋膜瓣与上唇皮肤做缝合固定,其余位置皮缘直接缝合。结果286例患者均于术后7 d拆线。所有患者术后随访12~24个月,重睑线形态自然流畅,闭眼时切口隐蔽,无凹陷性、“台阶样”瘢痕。满意度评价:280例满意,6例不满意,其中双侧重睑不对称3例,重睑线变浅3例,满意率为97.9%。结论眼轮匝肌“窗口法”切开重睑成形术是一种可靠稳定、瘢痕隐蔽、重睑效果自然流畅、接近仿生的重睑成形术,值得推广应用。

关键词：切开法重睑成形术眼轮匝肌窗口法

神经纤毛蛋白-1(NRP-1)在原发免疫性血小板减少症发病机制中的作用

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中华血液学杂志 2021年第2期42卷 146-150页

作者：周虎杨静宜徐佩佩刘柳丁冰洁刘建平李梦娟宋永平郑州大学附属肿瘤医院、河南省肿瘤医院血液科 450008 郑州大学第一附属医院血液科 450052

目的探讨神经纤毛蛋白-1(NRP-1)在调节性T细胞(Treg)上的表达与其配体信号素3A(Sema3A)、转化生长因子β1(TGF-β1)以及1型辅助T细胞(Th1)和2型辅助T细胞(Th2)之间平衡的关系。方法纳入2014年3月至2015年5月就诊的62例ITP患者(新诊断ITP... 详细信息

目的探讨神经纤毛蛋白-1(NRP-1)在调节性T细胞(Treg)上的表达与其配体信号素3A(Sema3A)、转化生长因子β1(TGF-β1)以及1型辅助T细胞(Th1)和2型辅助T细胞(Th2)之间平衡的关系。方法纳入2014年3月至2015年5月就诊的62例ITP患者(新诊断ITP 33例、慢性ITP 29例),以同期30名健康体检者作为正常对照组。流式细胞术检测Treg细胞NRP-1表达,酶联免疫吸附法(EliSA)检测血浆Sema3A、TGF-β1、IFN-γ和IL-4水平,实时聚合酶链式反应(RT-PCR)检测外周血NRP-1、Sema3A、TGF-β1 mRNA表达水平。分别采用单因素方差和独立样本t检验进行三组间和两组间比较,用Spearman相关系数评估NRP-1、Sema3A、TGF-β1 mRNA表达的相关性。结果与慢性ITP组和正常对照组比较,新诊断ITP组Treg细胞NRP-1表达降低[分别为(0.15±0.03)%、(0.33±0.15)%、(0.46±0.06)%,P<0.01],血浆Sema3A水平升高[分别为(8.10±1.32)μg/L、(7.41±1.30)μg/L、(2.88±0.82)μg/L,P<0.01],血浆TGF-β1水平降低[分别为(16.50±3.36)μg/L、(35.17±10.26)μg/L、(41.00±10.02)μg/L,P<0.01],血浆IFN-γ水平升高[分别为(17.21±2.80)ng/L、(10.23±1.59)ng/L、(8.18±3.27)ng/L,P<0.01],Th1/Th2(IFN-γ/IL-4)比值升高(分别为1.29±0.30、0.72±0.16、0.61±0.27,P<0.01)。新诊断ITP、慢性ITP组NRP-1、Sema3A mRNA的表达均低于正常对照组(P<0.01)。新诊断ITP组NRP-1 mRNA表达与Sema3A、TGF-β1 mRNA表达均呈正相关。结论NRP-1可能参与ITP的发病机制。

关键词：神经纤毛蛋白1 信号素3A 转化生长因子β1 原发免疫性血小板减少症

Cell polarization in ischemic stroke: molecular mechanisms and advances

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Neural Regeneration Research 2025年第3期20卷 632-645页

作者： Yuanwei li Xiaoxiao Xu Xuan Wu Jiarui li Shiling Chen Danyang Chen Gaigai li Zhouping Tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is a cerebrovascular disease associated with high mortality and disability rates. Since the inflammation and immune response play a central role in driving ischemic damage, it becomes essential to modulate excessive inflammatory reactions to promote cell survival and facilitate tissue repair around the injury site. Various cell types are involved in the inflammatory response, including microglia, astrocytes, and neutrophils, each exhibiting distinct phenotypic profiles upon stimulation. They display either proinflammatory or anti-inflammatory states, a phenomenon known as ‘cell polarization.’ There are two cell polarization therapy strategies. The first involves inducing cells into a neuroprotective phenotype in vitro, then reintroducing them autologously. The second approach utilizes small molecular substances to directly affect cells in vivo. In this review, we elucidate the polarization dynamics of the three reactive cell populations(microglia, astrocytes, and neutrophils) in the context of ischemic stroke, and provide a comprehensive summary of the molecular mechanisms involved in their phenotypic switching. By unraveling the complexity of cell polarization, we hope to offer insights for future research on neuroinflammation and novel therapeutic strategies for ischemic stroke.

关键词： astrocyte polarization immune regulation inflammation ischemic injury microglia polarization neutrophil polarization signaling pathways stroke

The Mechanism of Heating Rate on the Secondary Recrystallization Evolution in Grain Oriented Silicon Steel

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 275-282页

作者： GAO Qian li Jun WANG Xianhui CAO Laifu GONG Jian li Bo Central Iron and Steel Research Institute Group Beijing 100081China Shougang Zhixin Electromagnetic Material R&D(Beijing)Co. Ltd.Beijing 101300China Beijing Shougang Co. Ltd.Qian’an 064404China

Grain-oriented silicon steels were prepared at different heating rates during high temperature annealing,in which the evolution of magnetic properties,grain orientations and precipitates were *** illustrate the Zener factor,the diameter and number density of precipitates of interrupted testing samples were statistically *** effect of precipitate ripening on the Goss texture and magnetic property was *** indicated that the trend of Zener factor was similar under different heating rates,first increasing and then decreasing,and that the precipitate maturing was greatly inhibited as the heating rate *** recrystallization was developed at the temperature of 1010℃when a heating rate of 5℃/h was used,resulting in Goss,Brass and{110}oriented grains growing abnormally and a magnetic induction intensity of ***,increasing the heating rate to 20℃/h would inhibit the development of undesirable oriented grains and obtain a sharp Goss ***,when the heating rate was extremely fast,such as 40℃/h,poor secondary recrystallization was developed with many island grains,corresponding to a decrease in magnetic induction intensity to 1.87 *** a suitable heating rate of 20℃/h,the sharpest Goss texture and the highest magnetic induction of 1.94 T with an onset secondary recrystallization temperature of 1020℃were found among the experimental variables in this *** heating rate affected the initial temperature of secondary recrystallization by controlling the maturation of precipitates,leading to the deviation and dispersion of Goss texture,thereby reducing the magnetic properties.

关键词： high temperature annealing heating rate secondary recrystallization grain oriented silicon steel

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区块链溯源服务投入对生鲜供应链定价的影响

物流技术 2023年第1期42卷 98-106页

作者：刘盼李梦娟司海平 Fernando Bação 河南农业大学信息与管理科学学院河南郑州450046 新里斯本大学信息管理学院葡萄牙新里斯本1070-312

为研究生鲜供应链投资区块链溯源服务后如何定价才能获得更多收益,选取一个生鲜产品生产商和零售商组成的供应链为研究对象,考虑生鲜产品可追溯性和新鲜度的变化修正需求函数,并基于博弈理论,构建了四种收益模型。比较各模型下供应链的... 详细信息

为研究生鲜供应链投资区块链溯源服务后如何定价才能获得更多收益,选取一个生鲜产品生产商和零售商组成的供应链为研究对象,考虑生鲜产品可追溯性和新鲜度的变化修正需求函数,并基于博弈理论,构建了四种收益模型。比较各模型下供应链的收益,提出应用价格折扣和收益共享契约实现采用区块链溯源服务后的供应链协调。结果表明:一定条件下,价格折扣和收益共享契约可以刺激生产商和零售商的投资积极性,实现供应链协调;区块链溯源服务水平对生产商和零售商的定价策略影响敏感度与收益共享系数有关;生产商和零售商的最优定价、最优收益和感知新鲜度信息可信度与区块链溯源服务水平密切相关。供应链决策者投资区块链溯源服务后,可以通过提升感知新鲜度信息可信度提高收益。

关键词：区块链溯源服务生鲜供应链定价决策

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A Hybrid Approach for Pavement Crack Detection Using Mask R-CNN and Vision Transformer Model

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Computers, Materials & Continua 2025年第1期82卷 561-577页

作者： Shorouq Alshawabkeh li Wu Daojun Dong Yao Cheng liping li Faculty of Engineering China University of GeosciencesWuhan430074China

Detecting pavement cracks is critical for road safety and infrastructure *** methods,relying on manual inspection and basic image processing,are time-consuming and prone to *** deep-learning(DL)methods automate crack detection,but many still struggle with variable crack patterns and environmental *** study aims to address these limitations by introducing the Masker Transformer,a novel hybrid deep learning model that integrates the precise localization capabilities of Mask Region-based Convolutional Neural Network(Mask R-CNN)with the global contextual awareness of Vision Transformer(ViT).The research focuses on leveraging the strengths of both architectures to enhance segmentation accuracy and adaptability across different pavement *** evaluated the performance of theMaskerTransformer against other state-of-theartmodels such asU-Net,TransformerU-Net(TransUNet),U-NetTransformer(UNETr),SwinU-NetTransformer(Swin-UNETr),You Only Look Once version 8(YoloV8),and Mask R-CNN using two benchmark datasets:Crack500 and *** findings reveal that the MaskerTransformer significantly outperforms the existing models,achieving the highest Dice SimilarityCoefficient(DSC),precision,recall,and F1-Score across both ***,the model attained a DSC of 80.04%on Crack500 and 91.37%on DeepCrack,demonstrating superior segmentation accuracy and *** high precision and recall rates further substantiate its effectiveness in real-world applications,suggesting that the Masker Transformer can serve as a robust tool for automated pavement crack detection,potentially replacing more traditional methods.

关键词： Pavement crack segmentation transportation deep learning vision transformer Mask R-CNN image segmentation

Ultra‑High Sensitivity Anisotropic Piezoelectric Sensors for Structural Health Monitoring and Robotic Perception

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Nano-Micro Letters 2025年第2期17卷 432-446页

作者： Hao Yin Yanting li Zhiying Tian Qichao li Chenhui Jiang Enfu liang Yiping Guo State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Beijing Vacuum Electronics Research Institute Beijing 100015People’s Republic of China Fundamental Science On Vibration Shock and Noise LaboratoryState Key Laboratory of Mechanical System and VibrationSchool of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Monitoring minuscule mechanical signals,both in magnitude and direction,is imperative in many application scenarios,e.g.,structural health monitoring and robotic sensing ***,the piezoelectric sensor struggles to satisfy the requirements for directional recognition due to the limited piezoelectric coefficient matrix,and achieving sensitivity for detecting micrometer-scale deformations is also ***,we develop a vector sensor composed of lead zirconate titanate-electronic grade glass fiber composite filaments with oriented arrangement,capable of detecting minute anisotropic *** as-prepared vector sensor can identify the deformation directions even when subjected to an unprecedented nominal strain of 0.06%,thereby enabling its utility in accurately discerning the 5μm-height wrinkles in thin films and in monitoring human pulse *** ultra-high sensitivity is attributed to the formation of porous ferroelectret and the efficient load transfer efficiency of continuous lead zirconate titanate ***,when integrated with machine learning techniques,the sensor’s capability to recognize multi-signals enables it to differentiate between 10 types of fine textures with 100%*** structural design in piezoelectric devices enables a more comprehensive perception of mechanical stimuli,offering a novel perspective for enhancing recognition accuracy.

关键词： Flexible piezoelectric filaments Anisotropic Ultra-high sensitivity Structural health detection Texture recognition

Optimization Strategies of Na_(3)V_(2)(PO_(4))_(3) Cathode Materials for Sodium‑Ion Batteries

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Nano-Micro Letters 2025年第2期17卷 204-251页

作者： Jiawen Hu Xinwei li Qianqian liang li Xu Changsheng Ding Yu liu Yanfeng Gao School of Materials Science and Engineering Shanghai UniversityShanghai 200444People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China

Na_(3)V_(2)(PO_(4))_(3)(NVP)has garnered great attentions as a prospective cathode material for sodium-ion batteries(SIBs)by virtue of its decent theoretical capacity,superior ion conductivity and high structural ***,the inherently poor electronic conductivity and sluggish sodium-ion diffusion kinetics of NVP material give rise to inferior rate performance and unsatisfactory energy density,which strictly confine its further application in ***,it is of significance to boost the sodium storage performance of NVP cathode *** to now,many methods have been developed to optimize the electrochemical performance of NVP cathode *** this review,the latest advances in optimization strategies for improving the electrochemical performance of NVP cathode material are well summarized and discussed,including carbon coating or modification,foreign-ion doping or substitution and nanostructure and morphology *** foreign-ion doping or substitution is highlighted,involving Na,V,and PO_(4)^(3−)sites,which include single-site doping,multiple-site doping,single-ion doping,multiple-ion doping and so ***,the challenges and prospects of high-performance NVP cathode material are also put *** is believed that this review can provide a useful reference for designing and developing high-performance NVP cathode material toward the large-scale application in SIBs.

关键词： Sodium-ion batteries Na_(3)V_(2)(PO_(4))_(3) Cathode materials Electrochemical performance Optimization strategies