检索结果-南通市图书馆

In vivo Anti-tumor Effect of siRNA Against STAT3 on Transplanted Lewis Lung Cancer in Mice

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2008年第3期24卷 330-337页

作者： WANG Chun-guang WANG Rong-you SUN Mei li jin-dong GAO Nan ZHANG Xing-yi SHI dong-lei jin Cheng-yan Department of Thoracic Surgery the Second Hospital of Jilin University Changchun 130041 P. R. China Department of Pathology the Second Hospital of Jilin University Changchun 130041 P. R. China

This study aims at determining the therapeutic effect of knockdown of signal transducers and activators of transcription3（STAT3） gene expression by short interference RNA（siRNA） on transplanted Lewis lung cancer in mice in vivo. pSilencer 2.1-U6 STAT3 siRNA against STAT3（STAT3 siRNA） was synthesized. Lewis lung cancer cells were inoculated subcutaneously into C57BL/6 mice. Seven days after inoculation, the tumor-bearing mice were randomly divided into 3 groups and received intratumoral injection of （1） vehicle（PBS solution）, （2） vector（negative control）, and （3） STAT3 siRNA. Tumor volume and weight were calculated. Tumors and the lungs were excised for 21 days after inoculation. Expressions of STAT3, vascular endothelial growth factor（VEGF）, and matrix metalloproteinase-2（MMP2） were analyzed by RT-PCR, Western blot, and immunohistochemical staining. HE staining and TUNEL assay were used to confirm the apoptosis of tumors. The synthetic STAT3 siRNA effectively suppressed tumor growth, prevented tumor from pulmonary metastasis, and induced tumor apoptosis in vivo compared with vehicle and vectore in controls. It significantly inhibited STAT3 expression to contribute to downregulation of VEGF and MMP2 expression within tumors in vivo. This study demonstrates that STAT3 siRNA can effectively inhibit the expression of STAT3 gene within tumors, leading to suppression of tumor growth, prevention of cancer from pulmonary metastasis, and enhancing apoptosis of the transplanted Lewis lung cancer in mice in vivo.

关键词： STAT3 Lewis lung cancer siRNA

The Herbicidal Activity of Mutant Isolates from Botrytis cinerea

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2006年第8期5卷 622-628页

作者： ZHANG jin-lin ZHANG li-hui liU Ying-chao MA Juan li Chuan dong jin-gao Mycotoxin Laboratory Hebei Agricultural University Baoding 071001 P.R.China

Fifteen mutant isolates were obtained by ultraviolet mutation from parent isolate Botrytis cinerea BC-4. Among them three mutant isolates, BC4-1, BC4-2, and BC4-15, showed strong herbicidal activity. BC4-1 showed maximum herbicidal activity for inhibition of germination and growth of Digitaria sanguinalis L. and Arnaranthus retroflexus L. The results also showed that herbicidal activity was influenced by differing pH of PD media, with pH value of 4.0 being the optimum. The crude toxin was extracted using chloroform, petroleum ether, and ethyl acetate, respectively, and the ethyl acetate extracts showed the strongest inhibitory activity on the germination and growth of D. sanguinalis L. and A. retroflexus L. Using HPLC, one fraction with an absorption peak at 271 nm was separated from the crude toxin. This fraction could strongly inhibit the growth of D. sanguinalis L. at a concentration of 100 mg L^-1 and could completely inhibit the seed germination of D. sanguinalis L. and A. retroflexus L. at a concentration of 50 mg L^-1 .

关键词： Botrytis cinerea toxin ultraviolet mutation herbicide activity separation and purification

Methoxy poly(ethylene glycol)-b-poly(ethyl cyanoacrylate) copolymer nanoparticles as delivery vehicles for dexamethasone

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第17期54卷 2918-2924页

作者： ZHAI YingLei DENG liandong liN XiaoNa XIAO li jin FengMin dong AnJie School of Chemical Engineering and Technology Tianjin University 'l-ianjin 300072 China

Methoxy poly(ethylene oxide)-b-poly(ethyl cyanoacrylate) (mPEG-b-PECA), amphiphilic block copolymer, was synthesized via oxyanion-initiated polymerization with a sodium alcoholate-terminated monomethoxy poly(ethylene glycol) as the macroinitiator. mPEG-b-PECA was characterized by GPC, 1H-NMR and FTIR. The results indicate that the structure of mPEG-b-PECA is well controlled with narrow molecular weight distribution. The dexamethasone (DXM)-loaded mPEG-b-PECA nanoparticles (NPs) were prepared by the nanoprecipitation technique and characterized by LPSA, 1H-NMR and TEM. The DXM-loaded mPEG-b-PECA NPs are of spherical shape with the size of less than 100 nm. The drug-loaded amount (DL) and encapsulation efficiency (EE) of DXM-loaded NPs were investigated by HPLC. The results show that DXM can be effectively incorporated into mPEG-b-PECA NPs, which provides a potential delivery system for DXM and other hydrophobic drugs.

关键词：甲氧基聚乙二醇两亲嵌段共聚物 MPEG 地塞米松纳米粒子运载工具丙烯酸酯氰基丙烯酸乙酯

Construction and Characterization of Lentiviral shRNA Expression Vector Targeting Rat CD40 Gene in Dendritic Cells

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2009年第5期25卷 666-672页

作者： SUN Mei li jin-dong JIANG Rui jin Cheng-yan GAO Nan LUO Shu-li WANG Chun-guang WANG Bin WANG Rong-you ZHANG Xing-yi Department of Pathology Second Hospital of Jilin University Changehun 130041 P. R. China Department of Thoracic Surgery Second Hospital of Jilin University Changehun 130041 P. R. China Department of Orthopedic Surgery China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China Department of Thoracic Surgery China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China

To construct a lentiviral shRNA vector targeting rat CD40 gene and detect its effectiveness of gene silencing in dendritic cells（DCs）, specific siRNA targets with short hairpin frame were designed and synthesized according to the mRNA sequence of rat CD40 gene. DNA oligo was cloned into lentiviral expression vector, and then PCR and sequencing analyses were conducted to verify the constructs. The verified plasmids were transfected into 293T cells that over-express recombinant CD40 in order to select the most effective siRNA targets, shRNA lentiviruses from the selected constructs were propagated and harvested with a virus packaging system, and the virus titers were determined. Western blot and Real-time PCR were performed to determine CD40 expression level in the virusinfected dendritic cells. PCR and sequencing analyses reveal that shRNA plasmids of four targets were successfully constructed. The optimal interfering target was selected, and the virus with a titer of 5 × 10^7 TU/mL was successfully packaged. CD40 expression in rat DCs was knockdown at both mRNA and protein levels by virus infection. In comparison to that of control groups, CD40 mRNA expression and protein expression were decreased by 60.9% and 61.2%, respectively. We have successfully constructed recombinant lentiviral shRNA expression vector targeting rat CD40 gene that can effectively down-regulate CD40 gene expression at mRNA and protein levels in rat DC.

关键词： CD40 RNA interference Lentivirus Dendritic cell

Effect of Photoperiod Treatments on Dormancy Induction and Changes in Correlated Respiratory Rate of Nectarine Peach Bud

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第12期7卷 1445-1450页

作者： li jin GAO dong-sheng ZHAO Kai YU Qin XU Chen-shan State Key Laboratory of Crop Biology College of Horticulture Science and Engineering Shandong Agricultural University Tai＇an271018 P.R.China

The responses of dormancy induction to illumination and the characteristics of respiratory rate were studied with the nectarine peach bud in this article. The trial was conducted with nectarine （Prunus persica var. nectariana cv. Shuguang） and involved three treatments： a short day treatment （8 h）, a long day treatment （16 h）, and the normal condition as the control. The dormancy status was determined with the growth of shoot and the sprouting ability, and the respiratory rate was mensurated with oxygen electrode. Short day treatment could induce the growth stopping of peach shoots ahead, promote the development of dormancy, and induce buds into dormancy with 21 d previous to control. Long day treatment postponed the growth stopping and the induction and development of dormancy. The respiratory rate decreased according to the development of dormancy induction. The minimum respiratory rate appeared about 7 days after the start of dormancy induction. Bud respiratory rate increased during this period and then declined and remained at low level during dormancy period. Long day reduced buds respiratory rate slightly. Short day could induce dormancy obviously, and long day postponed dormancy induction. The changes of respiratory rate were correlated with the development of dormancy induction, and the bud respiratory rate was also affected by photoperiod.

关键词： peach dormancy induction photoperiod respiratory rate

Alveolar Rhabdomyosarcoma of the Laryngohypopharynx： A Case Report

在线全文

学校读者我要写书评

暂无评论

Clinical oncology and cancer researeh 2010年第1期7卷 69-70页

作者： Da-wei li jin XIE Pin dong Deptartpent of Otorhinolaryngology & Headand Neck the First Affiliated Hospital ofShanghai Jiaotong University Shanghai200080. China.

Introduction Rhabdomyosarcoma is one of the most commonly seen soft-tissue sarcomas in children and teenagers, approximately accounting for half of all soft-tissue sarcomas. The tumor is usually found in head and neck, four limbs and urogenital system. However, the tumor orig- inating from the laryngohypopharynx is rare. In 2008, a patient with alveolar rhabdomyosarcoma of the laryngohypopharynx was admitted to our hospital. Clinical information of the disease was reported as follows, and clinicopathologic features and current therapeutic principle were discussed and summarized in combination with the review of literature, to facilitate the diagnosis and treatment of the patients with rhabdomyosarcoma of the laryngohypopharynx in future.

关键词： laryngeal neoplasm rhabdomyosarcoma diagnosis therapy.

Expression and Characterization of Catalytic Domain of T Cell Protein Tyrosine Phosphatase(ΔTC-PTP)——Immunohistochemical Study of ΔTC-PTP Expression in Non-small Cell Lung Carcinomas

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第3期23卷 289-296页

作者： ZHU Zhi-cheng SUN Mei ZHANG Xing-yi liU Ke-xiang SHI dong-lei li jin-dong SU Ji-quan XU Yue-chi FU Xue-qi Department of Cardiovascular Surgery The Second Hospital of Jilin University Changchun 130041 P. R. China Department of Pathology The Second Hospital of Jilin University Changchun 130041 P. R. China Department of Thoracic Surgery The Second Hospital of Jilin University Changchun 130041 P. R. China College of life Sciences Jilin University Changchun 130023 P. R. China

This study objective was to express and characterize the catalytic domain of the human T cell protein tyrosine phosphatase（△TC-PTP） and to study immunohistochemically the expression of △TC-PTP in human non-small cell lung cancers. △TC-PTP gene was PCR amplified with the cDNA of human TC-PTP as template, and cloned into the pT7 expression vector. The recombinant pT7-△TC-PTP was expressed in E. coli Rosetta （ DE3 ） host cells and puri- fied. The enzymatic characteristics of △TC-PTP including enzyme activity and kinetics assay were measured. The antiserum was prepared by immunizing rabbit with the purified recombinant △TC-PTP. Rabbit polyclonal antibody against △TC-PTP was purified by PVDF immobilized antigen affinity chromatography. Immunohistochemical staining of lung cancer tissues was performed with antibody against △TC-PTP protein. △TC-PTP gene was correctly cloned, expressed, and purified. The recombinant △TC-PTP had a highly catalytic activity of PTPase. Squamous cell lung carcinoma showed a significantly higher expression rate of △TC-PTP （76. 92%, 10/13 ） than adenocarcinoma （57.14%, 4/7） and normal lung tissue（20%, 1/5 ）. This study represents the first demonstration that △TC-PTP is highly expressed in human squamous cell lung carcinomas. In addition, this study provides an important basis for further studying the biological function of TC-PTP and its relationship with lung carcinomas and other diseases.

关键词： T cell protein tyrosine phosphatase Catalytic domain Characterization Lung cancer

Normality Family and Shared Functions by Meromorphic Functions and Its Differential Polynomials

在线全文

学校读者我要写书评

暂无评论

Journal of Mathematical Research and Exposition 2008年第3期28卷 541-548页

作者： LU Qian li jin dong College of Science Southwest University of Science and Technology Sichuan 621010 China College of Information Management Chengdu University of Technology Sichuan 610059 China

In this paper, we obtain the following normal criterion： Let F be a family of mero-morphic functions in domain D belong to C, all of whose zeros have multiplicity k ＋ 1 at least. If there exist holomorphic functions α（z） not vanishing on D, such that for every function f（z） ∈F, f（z） shares α（z） IM with L（f） on D, then F is normal on D, where L（f） is linear differential polynomials of f（z） with holomorphic coefficients, and k is some positive numbers. We also proved coressponding results on normal functions.

关键词： meromorphic functions differential polynomials normal criterion shared functions.

在线全文

学校读者我要写书评

暂无评论

固液相变过程最优控制数学定解分析及其算法

工程热物理学报 2005年第1期26卷 91-94页

作者：李劲东中国空间技术研究院总体专业技术部北京100086

本文应用分布参数系统控制理论,采用变分法系统地分析,导出了二维固液相变传热过程最优控制问题的数学定解条件,提出模型离散和数值求解方法,对共轭梯度最优控制算法进行适应性分析。

关键词：固液相变过程最优控制