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The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice

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Molecular Plant 2021年第6期14卷 1012-1023页

作者： Qing wang Qingmei Su Jinqiang Nian Jian Zhang Meng Guo Guojun Dong Jiang Hu Rongsheng wang Changshuo Wei Guanwen li Wan wang Hui-Shan Guo Shaoyang lin Wenfeng Qian XianzhiXie Qian Qian Fan Chen jianru Zuo State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Chinese Research Academy of Environmental Sciences Beijing 100012China Shandong Rice Research Institute Shandong Academy of Agricultural SciencesJinan 250100China Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesBeijing 100101China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

The genetic improvement of nitrogen use efficiency(NUE)of crops is vital for grain productivity and sustainable ***,the regulatory mechanism of NUE remains largely ***,we report that the rice Grain number,plant height,and heading date7(Ghd7)gene genetically acts upstream of ABC1 REPRESSOR1(ARE1),a negative regulator of NUE,to positively regulate nitrogen *** a transcriptional repressor,Ghd7 directly binds to two Evening Element-like motifs in the promoter and intron 1 of ARE1,likely in a cooperative manner,to repress its ***7 and ARE1 display diurnal expression patterns in an inverse oscillation manner,mirroring a regulatory scheme based on these two *** of a panel of 2656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during ***,the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South ***,the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting ***,these results define a Ghd7–ARE1-based regulatory mechanism of nitrogen utilization,providing useful targets for genetic improvement of rice NUE.

关键词： elite crops heading

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南海南部陆坡表层沉积物细菌和古菌多样性

微生物学报 2008年第3期48卷 323-329页

作者：李涛王鹏汪品先同济大学海洋地质国家重点实验室上海200092

从南海南部陆坡表层沉积物中扩增了细菌和古菌16S rDNA序列,并对克隆子文库进行系统发育分析。细菌序列以变形杆菌(Proteobacteria)居多,其次是浮霉菌(Planctomycete)、酸杆菌(Acidobacteria)和candidate division OP10,另外还有少量铁... 详细信息

从南海南部陆坡表层沉积物中扩增了细菌和古菌16S rDNA序列,并对克隆子文库进行系统发育分析。细菌序列以变形杆菌(Proteobacteria)居多,其次是浮霉菌(Planctomycete)、酸杆菌(Acidobacteria)和candidate division OP10,另外还有少量铁还原杆菌(Deferrobacteres)、candidate di-vision OP3、OP11、OP8、TM6、疣微菌(Verrucomicrobia)和螺旋体(Spirochaetes)。古菌序列分别来自泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota),以Marine Benthic Group B(MBGB)、Marine Crenarchaeotic GroupⅠ(MGⅠ)、Marine Benthic Group D(MBGD)和South African Gold Mine Eu-ryarchaeotic Group(SAGMEG)为主。少量序列为C3、甲烷杆菌(Methanobacteriales)和Novel Eu-ryarchaeotic Group(NEG)。结果表明海底表层沉积物中有丰富多样的微生物群落。

关键词：南海沉积物 16S rDNA 多样性

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95家危重新生儿救治中心建设现状调查

发育医学电子杂志 2024年第2期12卷 102-107页

作者：彭健茹韩涛李秋平杨常栓张艳平张珊李磊王自珍朱柠孔祥永惠州市中心人民医院新生儿科广东惠州516000 解放军总医院儿科医学部解放军总医院第七医学中心新生儿重症监护病房北京100700 南方医科大学第二临床医学院广东广州510000

目的评估《危重新生儿救治中心建设与管理指南》(以下简称《指南》)在指导危重新生儿救治中心(以下简称“救治中心”)资源建设上的有效性、针对性和可操作性。方法2019年6月至12月,在我国东、中、西部地区共选取95家救治中心,其中省级25... 详细信息

目的评估《危重新生儿救治中心建设与管理指南》(以下简称《指南》)在指导危重新生儿救治中心(以下简称“救治中心”)资源建设上的有效性、针对性和可操作性。方法2019年6月至12月,在我国东、中、西部地区共选取95家救治中心,其中省级25家,市级35家,县级35家,对危重新生儿救治网络建设现状进行调查。调查内容包括病房建设、设备配置和开展的技术项目。统计学方法采用χ^(2)检验或Fisher确切概率法。结果25家省级救治中心均在三级甲等医院,57%(20/35)的市级救治中心在三级甲等医院。省、市、县级救治中心床位数达标率分别为64%(16/25)、71%(25/35)、89%(31/35)。95家救治中心均能开展新生儿复苏和蓝光治疗。25家省级救治中心均能开展普通氧疗、气管插管、无创监测、血气分析、持续气道正压通气(continuous positive airway pressure,CPAP),但亚低温治疗、腹膜透析、连续性肾脏替代治疗(continuous renal replacement therapy,CRRT)等技术项目的达标率较低。部分市级救治中心未按《指南》要求开展CPAP、高频通气、换血疗法和经外周置入中心静脉导管(peripherally inserted central catheter,PICC)。部分县级救治中心的基本技术尚未达标,如气管插管、无创监测、普通氧疗等。省、市、县3级比较,新生儿复苏、气管插管、CPAP、蓝光治疗、血气分析等技术项目的达标率差异无统计学意义(P>0.05);而换血疗法、无创监测、普通氧疗、PICC等技术项目比较,差异有统计学意义(P<0.05),省级达标率较高。结论部分救治中心的床位设置、设备配置、技术项目需要进一步完善,以推动新生儿救治中心及区域协作救治网络体系的建设。

关键词：危重新生儿救治中心指南现状调查技术项目

SIRT7 orchestrates melanoma progression by simultaneously promoting cell survival and immune evasion via UPR activation

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1953-1969页

作者： Xiuli Yi Huina wang Yuqi Yang Hao wang Hengxiang Zhang Sen Guo jianru Chen Juan Du Yangzi Tian Jingjing Ma Baolu Zhang lili Wu Qiong Shi Tianwen Gao Weinan Guo Chunying Department of Dermatology Xijing HospitalFourth Military Medical UniversityNo 127 of West Changle RoadXi’anShaanxi 710032China

Melanoma is the most lethal type of skin cancer,originating from the malignant transformation of *** the development of targeted therapy and immunotherapy has gained revolutionary advances in potentiating the therapeutic effect,the prognosis of patients with melanoma is still *** tumor progression,melanoma frequently encounters stress from both endogenous and exogenous sources in tumor ***7 is a nuclear-localized deacetylase of which the activity is highly dependent on intracellular nicotinamide adenine dinucleotide(NAD+),with versatile biological functions in maintaining cell ***,whether SIRT7 regulates tumor cell biology and tumor immunology in melanoma under stressful tumor microenvironment remains ***,we reported that SIRT7 orchestrates melanoma progression by simultaneously promoting tumor cell survival and immune evasion via the activation of unfolded protein *** first identified that SIRT7 expression was the most significantly increased one in sirtuins family upon ***,we proved that the deficiency of SIRT7 potentiated tumor cell death under stress in vitro and suppressed melanoma growth in ***,SIRT7 selectively activated the IRE1α-XBP1 axis to potentiate the pro-survival ERK signal pathway and the secretion of tumorpromoting ***7 directly de-acetylated SMAD4 to antagonize the TGF-β-SMAD4 signal,which relieved the transcriptional repression on IRE1αand induced the activation of the IRE1α-XBP1 ***,SIRT7 up-regulation eradicated anti-tumor immunity by promoting PD-L1 expression via the IRE1α-XBP1 ***,the synergized therapeutic effect of SIRT7 suppression and anti-PD-1 immune checkpoint blockade was also *** together,SIRT7 can be employed as a promising target to restrain tumor growth and increase the effect of melanoma immunotherapy.

关键词： melanoma SIRT7 immunity

Regulation of nitrogen starvation responses by the alarmone(p)ppGpp in rice

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Journal of Genetics and Genomics 2022年第5期49卷 469-480页

作者： Hanwen li Jinqiang Nian Shuang Fang Meng Guo Xiahe Huang Fengxia Zhang Qing wang Jian Zhang Jiaoteng Bai Guojun Dong Peiyong Xin Xianzhi Xie Fan Chen Guodong wang Yingchun wang Qian Qian jianru Zuo Jinfang Chu Xiaohui Ma State Key Laboratory of Plant Genomics National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhouZhejiang 310006China Institute of Wetland Agriculture and Ecology Shandong Academy of Agricultural SciencesJinanShandong 250100China Hainan Yazhou Bay Laboratory SanyaHainan 572025China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesBeijing 100101China

Nitrogen is an essential macronutrient for all living organisms and is critical for crop productivity and *** higher plants,inorganic nitrogen is absorbed through roots and then assimilated into amino acids by the highly conserved glutamine synthetase/glutamine:2-oxoglutarate aminotransferase(GS/GOGAT)*** nitrogen metabolism and nitrogen starvation responses of plants are regulated remains largely *** studies revealed that mutations in the rice ABNORMAL CYTOKININ RESPONSE1(ABC1)gene encoding Fd-GOGAT cause a typical nitrogen deficiency ***,we show that ARE2(for ABC1 REPRESSOR2)is a key regulator of nitrogen starvation responses in *** are2 mutations partially rescue the nitrogen-deficient phenotype of abc1 and the are2 mutants show enhanced tolerance to nitrogen deficiency,suggesting that ARE2 genetically interacts with ABC1/***2 encodes a chloroplast-localized Rel A/Spo T homolog protein that catalyzes the hydrolysis of guanosine pentaphosphate or tetraphosphate(p)pp Gpp,an alarmone regulating the stringent response in bacteria under nutritional stress *** are2 mutants accumulate excessive amounts of(p)pp Gpp,which correlate with lower levels of photosynthetic proteins and higher amino acid ***,these observations suggest that the alarmone(p)pp Gpp mediates nitrogen stress responses and may constitute a highly conserved mechanism from bacteria to plants.

关键词： Rice Stringent response Nitrogen starvation response (p)ppGpp ARE2

A novel molecular classification method for osteosarcoma based on tumor cell differentiation trajectories

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Bone Research 2023年第1期11卷 148-162页

作者： Hao Zhang Ting wang Haiyi Gong Runyi Jiang wang Zhou Haitao Sun Runzhi Huang Yao wang Zhipeng Wu Wei Xu Zhenxi li Quan Huang Xiaopan Cai Zaijun lin Jinbo Hu Qi Jia Chen Ye Haifeng Wei jianru Xiao Department of Orthopedic Oncology Shanghai Changzheng HospitalNaval Military Medical UniversityShanghai200003China Musculoskeletal Laboratory Institute of BiotechnologyUniversity of Shanghai for Science and TechnologyShanghai200093China Department of Orthopedics Naval Medical Center of CPLANaval Medical UniversityShanghai200052China Peking-Tsinghua Center for life Sciences Tsinghua UniversityBeijing100084China Division of Spine Department of OrthopedicsTongji Hospital Affiliated to Tongji University School of MedicineShanghai200065China

Subclassification of tumors based on molecular features may facilitate therapeutic choice and increase the response rate of cancer ***,the highly complex cell origin involved in osteosarcoma(OS)limits the utility of traditional bulk RNA sequencing for OS ***-cell RNA sequencing(sc RNA-seq)holds great promise for identifying cell ***,this technique has rarely been used in the study of tumor *** analyzing sc RNA-seq data for six conventional OS and nine cancellous bone(CB)samples,we identified 29 clusters in OS and CB samples and discovered three differentiation trajectories from the cancer stem cell(CSC)-like subset,which allowed us to classify OS samples into three *** classification model was further examined using the TARGET *** subgroup of OS had different prognoses and possible drug sensitivities,and OS cells in the three differentiation branches showed distinct interactions with other clusters in the OS *** addition,we verified the classification model through IHC staining in 138 OS samples,revealing a worse prognosis for Group B ***,we describe the novel transcriptional program of CSCs and highlight the activation of EZH2 in CSCs of *** findings provide a novel subclassification method based on sc RNA-seq and shed new light on the molecular features of CSCs in OS and may serve as valuable references for precision treatment for and therapeutic development in OS.

关键词： osteosarcoma holds classify

Quaternary biogenic opal records in the South China Sea: linkages to East Asian monsoon, global ice volume and orbital forcing

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Science China Earth Sciences 2007年第5期50卷 710-724页

作者： wang RuJian JIAN ZhiMin XIAO WenShen TIAN Jun li jianru CHEN RongHua ZHENG YuLong CHEN JianFang State Key Laboratory of Marine Geology Tongji University Shanghai 200092 China

Particulate fluxes investigated in the central South China Sea (SCS) during 1993―1996 indicate that opal flux can be used to show primary productivity change, which provides a foundation for tracing the evolutionary relationship between the surface productivity and East Asian monsoon in the SCS during the late Quaternary glacial and interglacial periods. Based on the studies of opal % and their mass accumulation rates (MAR) at the six sites recovered from the SCS during the “Resolution” ODP Leg 184 and “Sonne” 95 cruise of the Sino-Germany cooperation, opal % and their MARs increased evidently in the northern sites since 470―900 ka, and they enhanced and reduced, respectively, during the glacial and interglacial periods. Whereas they increased obviously in the southern sites since 420―450 ka, and they augmented and declined, respectively, during the interglacial and glacial periods. The vari- ability in opal % and their MARs in the late Quaternary glacial cyclicity indicate the “seesaw” pattern of surface productivity in the SCS. The winter monsoon intensified during the glacial periods, surface productivity increased and decreased, respectively, in the northern and southern SCS. The summer monsoon strengthened during the interglacial periods, surface productivity increased and decreased, respectively, in the southern and northern SCS. The cross spectral analyses between the opal % in the northern and southern SCS during the Quaternary and global ice volume (δ 18O) and orbital forcing (ETP) indicate that the East Asian winter and summer monsoons could be ascribed to the different drive mechanisms. On the orbital time scale, the global ice volume change could be a dominant factor for the winter monsoon intension and temporal variations. As compared with the winter monsoon, the correlative summer solar radiation with the obliquity and precession in the Northern Hemisphere could be a mostly controlling factor for the summer monsoon intension and temporal variations.

关键词： biogenic opal surface productivity East Asian monsoon global ice volume orbital forcing ODP Leg 184 Quaternary South China Sea

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荫罩式彩显管电子束斑测量技术的研究

电子学报 1999年第6期27卷 13-15页

作者：于莉杜秉初汪健如汪荣军清华大学电子工程系北京100084

本文提出了实时地、真实地再现荫罩式彩显管电子束斑的原理和方法；描述了系统的硬件组成；微偏转系统的要求和设计原则；并利用图形相关原理，实现了微偏转系统偏转灵敏度的校准．最后给出了在有荫罩隔离的情况下，在彩显管荧光屏前测... 详细信息

本文提出了实时地、真实地再现荫罩式彩显管电子束斑的原理和方法；描述了系统的硬件组成；微偏转系统的要求和设计原则；并利用图形相关原理，实现了微偏转系统偏转灵敏度的校准．最后给出了在有荫罩隔离的情况下，在彩显管荧光屏前测出的完美电子束斑．

关键词：阴极射线管 CRT 彩色显象管束斑测量

Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop

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Bone Research 2020年第2期8卷 169-181页

作者： Fan Chen Guowei Jiang Hui liu Zemin li Yuxin Pei Hua wang Hehai Pan Haowen Cui Jun Long jianru wang Zhaomin Zheng Department of Spine Surgery The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Department of Pediatric Intensive Care Unit The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Pain Research Center Sun Yat-Sen UniversityGuangzhou 510080China

The inflammatory response is induced by the overexpression of inflammatory cytokines, mainly interleukin(IL)-1β, and is one of the main causes of intervertebral disc degeneration(IVDD). NLR pyrin domain containing 3(NLRP3) inflammasome activation is an important source of IL-1β. As an anti-inflammatory neuroendocrine hormone, melatonin plays various roles in different pathophysiological conditions. However, its roles in IVDD are still not well understood and require more examination. First, we demonstrated that melatonin delayed the progression of IVDD and relieved IVDD-related low back pain in a rat needle puncture IVDD model;moreover, NLRP3 inflammasome activation(NLRP3, p20, and IL-1β levels) was significantly upregulated in severely degenerated human discs and a rat IVDD model. Subsequently, an IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop was found in nucleus pulposus(NP) cells that were treated with IL-1β. In these cells, expression of NLRP3 and p20 was significantly increased, NF-κB signaling was involved in this regulation, and mitochondrial reactive oxygen species(mt ROS)production increased. Furthermore, we found that melatonin disrupted the IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop in vitro and in vivo. Melatonin treatment decreased NLRP3, p20, and IL-1β levels by inhibiting NF-κB signaling and downregulating mt ROS production. Finally, we showed that melatonin mediated the disruption of the positive feedback loop of IL-1β in vivo. In this study, we showed for the first time that IL-1β promotes its own expression by upregulating NLRP3 inflammasome activation. Furthermore, melatonin disrupts the IL-1β positive feedback loop and may be a potential therapeutic agent for IVDD.

关键词： NLRP3 degeneration inflam