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第三届中国畜牧基因组产业转化高峰论坛

作者： Sheng Lu Yang liu Feng liu Changwei Shao Qian Zhou Na Wang yangzhen li Yingming Yang Yingping Zhang Hejun Sun Weiwei Zheng Songlin Chen Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences (CAFS) Key Laboratory for Sustainable Development of Marine Fisheries Ministry of Agriculture Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology College of Marine life Science Ocean University of China Wuxi Fisheries College Nanjing Agricultural University Marine and Fishery Institute of Zhejiang Ocean University

Japanese flounder is one of the most widely farmed economic flatfish species throughout eastern Asia including China,Korea,and *** tarda is a major species of pathogenic bacteria that causes ascites disease and consequently,a huge economy loss for Japanese flounder *** generations selection,traditional breeding methods can hardly improve the *** resistance *** selection is an effective way to predict the breeding potential of parents but has rarely been used in aquatic *** this study,we chose931 individuals from 90 families,challenged by *** from 2013-2015 as a reference population and 71 parents of these families as selection candidates.1,934,475 markers were detected via genome sequencing and applied in this *** different algorithms,the Bayes Cπand GBLUP were used for genomic selection *** the reference population,two algorithms let to the same result（0.904）and Pearson’s correlation results between EBV andGEBV of Bayes Cπand GBLUP were 0.706 and 0.795,*** selection candidates,GEBV from two algorithms were extremely similar（0.957）.Comparing GEBV with the survival rate of families structured by selection candidates,the correlation was 0.682 and 0.665,*** of different groups divided by survival rate revealed significant differences,and the group with the higher survival rate had higher mean *** study established genomic selection method for the Japanese flounder and for the first time applied these to *** resistance ***,we provide a new breeding technique for aquatic breeding.

关键词： genomic selection Bayes Cπ GBLUP Edwardsiella tarda Japanese flounder

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基于结构化处理的评论文本情感量化分析模型

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江西理工大学学报 2016年第3期37卷 83-90页

作者：廖列法李养振黎晨江西理工大学信息工程学院江西赣州341000

由于传统褒贬二值分类模型缺乏对文本主题之间以及主题与观点持有者之间的关系挖掘,不能很好的处理具有不规则、口语化、极性强等特点的评论文本.通过提取网站的文本评论,对评论对象进行结构化处理,以How Net公布的情感词典为基础,完善... 详细信息

由于传统褒贬二值分类模型缺乏对文本主题之间以及主题与观点持有者之间的关系挖掘,不能很好的处理具有不规则、口语化、极性强等特点的评论文本.通过提取网站的文本评论,对评论对象进行结构化处理,以How Net公布的情感词典为基础,完善了评论情感倾向性词典.结合五元组模型量化文本情感信息,建立了适合处理评论文本的模型,深度挖掘了用户对商品或消费行为的主观感受.并通过实验验证了该模型的准确度和有效性.

关键词：情感分析观点挖掘自然语言处理评论文本五元组知网词典

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单宁酶发酵生产的研究进展

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中国酿造 2008年第6期27卷 1-6页

作者：李秧针邱树毅保玉心王明力贵州省发酵工程与生物制药重点实验室

单宁酶可水解没食子酸单宁中的酯键和缩酚羧键,生成没食子酸和葡萄糖。单宁酶广泛应用于制革、酿酒、医药、饮料等领域。文中综述了国内外有关单宁酶发酵生产的研究进展,包括产单宁酶菌种的选育、发酵生产方法、底物选择和培养基中添加... 详细信息

单宁酶可水解没食子酸单宁中的酯键和缩酚羧键,生成没食子酸和葡萄糖。单宁酶广泛应用于制革、酿酒、医药、饮料等领域。文中综述了国内外有关单宁酶发酵生产的研究进展,包括产单宁酶菌种的选育、发酵生产方法、底物选择和培养基中添加剂对产酶的影响。

关键词：单宁酶菌种选育发酵方法底物

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Locus mapping, molecular cloning and expression analysis of rps6kb2, a novel metamorphosis-related gene in Chinese tongue sole（Cynoglossus semilaevis）

Locus mapping, molecular cloning and expression analysis of ...

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第三届中国畜牧基因组产业转化高峰论坛

作者： Yang liu Min Wei Hua Guo Changwei Shao liang Meng Wenteng Xu Na Wang Lei Wang Deborah M Power Jilun Hou Shahid Mahboob Zhongkai Cui Yingming Yang yangzhen li Fazhen Zhao Songlin Chen Wuxi Fisheries College Nanjing Agricultural University Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences (CAFS) Key Laboratory for Sustainable Development of Marine Fisheries Ministry of Agriculture Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology College of Fisheries and life Science Shanghai Ocean University Ministry of Education Beidaihe Central Experiment Station Chinese Academy of Fishery Sciences Department of Zoology College of Science King Saud University Department of Zoology GC University

Flatfish metamorphosis denotes the extraordinary transformation of a symmetric pelagic larva into an asymmetric benthic *** unique process involves eye migration,a90°rotation in posture and asymmetrical pigmentation for adaptation to a benthic *** the present study,we used genetics to map a metamorphosis related-locus（q-10M）on a male linkage group（LG10M）,a small interval of 0.9 c M corresponding to a 1.8Mb physical area in chromosome 9 in the Chinese tongue sole（Cynoglossus semilaevis）.Combined with single marker analysis,ribosomal protein S6 kinase 2（rps6kb2）a member of the family of AGC kinases was identified as a potential metamorphosis-related candidate *** expression pattern during metamorphosis was determined by quantitative RT-PCR and whole-mount in situ hybridization ***6kb2 gene was significantly expressed in metamorphic climax stage larvae and distributed in all tissues transforming during metamorphosis,including tail,jaw,eye and skin of *** results suggest that rps6kb2 has a general role in tissue transformations during flatfish metamorphosis including tail changes,skull remodeling,eye migration and asymmetrical pigmentation.

关键词： Cynoglossus semilaevis metamorphosis Simple sequence repeat(SSR) Locus mapping Ribosomal protein S6 kinase 2(rps6kb2)

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半滑舌鳎酪氨酸酶基因(TYR)和多巴色素异构酶基因(DCT)的克隆表达与分析

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渔业科学进展 2021年第6期42卷 42-52页

作者：吴垚磊李仰真王娜马腾赵玉柱陈松林中国水产科学研究院黄海水产研究所农业农村部海洋渔业可持续发展重点实验室青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛266071 上海海洋大学水产科学国家级实验教学示范中心上海201306

作为我国鲆鳎鱼类中的一种主要养殖品种,半滑舌鳎(Cynoglossus semilaevis)有时会发生无眼侧黑化(Melanism)、有眼侧白化(Albinism)的体色异常现象。本研究克隆了半滑舌鳎体色相关的酪氨酸酶基因(TYR)和多巴色素异构酶基因(DCT)的cDNA序... 详细信息

作为我国鲆鳎鱼类中的一种主要养殖品种,半滑舌鳎(Cynoglossus semilaevis)有时会发生无眼侧黑化(Melanism)、有眼侧白化(Albinism)的体色异常现象。本研究克隆了半滑舌鳎体色相关的酪氨酸酶基因(TYR)和多巴色素异构酶基因(DCT)的cDNA序列,并对这2个基因进行系统发育分析和时空表达分析。实验获得了TYR基因编码区cDNA序列长度为1620 bp,编码539个氨基酸;DCT基因编码区cDNA序列长度为1551 bp,编码516个氨基酸。结果显示,TYR和DCT在20日龄前的鱼苗体内表达量较高,尤其是在变态关键时期(15~20日龄)表达量最高,30日龄时的表达量锐减到很低水平;在其他时期的皮肤组织中,这2个基因在有眼侧正常皮肤和无眼侧黑化皮肤的表达量最高,在有眼侧白化皮肤和无眼侧正常皮肤中表达量极低;在其他时期的其他组织中,在眼睛中的表达量最高,其次是肝脏,在脾脏和肌肉中表达量极低。研究表明,TYR和DCT基因是半滑舌鳎无眼侧黑化发生和有眼侧体色维持的关键基因。本研究为查明半滑舌鳎体色异常机制提供了重要依据和参考。

关键词：半滑舌鳎体色酪氨酸酶基因(TYR) 多巴色素异构酶基因(DCT)

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Interpretable Machine Learning Method for Compressive Strength Prediction and Analysis of Pure Fly Ash-based Geopolymer Concrete

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 65-78页

作者： SHI Yuqiong li Jingyi ZHANG Yang li li Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of Ministry of Education Northwest A&F Uni-versityYangling 712100China College of Water Resources and Architectural Engineering Northwest A&F UniversityYangling 712100China Chongqing Key Laboratory of Public Big Data Security Technology Chongqing 401420China Chongqing College of Mobile Communication Chongqing 401520China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina National Rail Transit Electrification and Automation Engineering Technology Research Center(Hong Kong Branch) The Hong Kong Polytechnic UniversityKowloonHung HomHong KongChina

In order to study the characteristics of pure fly ash-based geopolymer concrete(PFGC)conveniently,we used a machine learning method that can quantify the perception of characteristics to predict its compressive *** this study,505 groups of data were collected,and a new database of compressive strength of PFGC was *** order to establish an accurate prediction model of compressive strength,five different types of machine learning networks were used for comparative *** five machine learning models all showed good compressive strength prediction performance on *** them,R2,MSE,RMSE and MAE of decision tree model(DT)are 0.99,1.58,1.25,and 0.25,*** R2,MSE,RMSE and MAE of random forest model(RF)are 0.97,5.17,2.27 and 1.38,*** two models have high prediction accuracy and outstanding generalization *** order to enhance the interpretability of model decision-making,we used importance ranking to obtain the perception of machine learning model to 13 *** 13 variables include chemical composition of fly ash(SiO_(2)/Al_(2)O_(3),Si/Al),the ratio of alkaline liquid to the binder,curing temperature,curing durations inside oven,fly ash dosage,fine aggregate dosage,coarse aggregate dosage,extra water dosage and sodium hydroxide *** temperature,specimen ages and curing durations inside oven have the greatest influence on the prediction results,indicating that curing conditions have more prominent influence on the compressive strength of PFGC than ordinary Portland cement *** importance of curing conditions of PFGC even exceeds that of the concrete mix proportion,due to the low reactivity of pure fly ash.

关键词： machine learning pure fly ash geopolymer compressive strength feature perception

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半滑舌鳎lnc-XR＿003049606.1及其靶基因pmelb在无眼侧皮肤黑化过程中的表达

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水产学报 2022年第4期46卷 529-536页

作者：胡元日杨倩王佳林李仰真许岩岩崔忠凯程鹏陈松林上海海洋大学水产与生命学院中国水产科学研究院黄海水产研究所海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室山东省海洋渔业生物技术与遗传育种重点实验室唐山市农业科学研究院

为探究人工养殖半滑舌鳎无眼侧皮肤经常出现黑化现象的成因，实验根据本课题组前期全转录组数据分析结果预测得出lnc-XR＿003049606.1与pmelb在基因层面上存在反式调控的关系。在半滑舌鳎无眼侧黑化皮肤中克隆获得pmelb的c DNA序列。生... 详细信息

为探究人工养殖半滑舌鳎无眼侧皮肤经常出现黑化现象的成因，实验根据本课题组前期全转录组数据分析结果预测得出lnc-XR＿003049606.1与pmelb在基因层面上存在反式调控的关系。在半滑舌鳎无眼侧黑化皮肤中克隆获得pmelb的c DNA序列。生物信息学分析结果显示，pmelb的c DNA序列全长1 755 bp，编码584个氨基酸，其预测的蛋白分子量为63.94 ku，理论等电点为5.12，预测的二级蛋白结构含有1个Pleckstrin同源域、1个布鲁顿酪氨酸激酶富胱氨酸基序、1个Src同源3域、1个Src同源2域和1个酪氨酸激酶催化结构域。系统进化树分析显示，pmelb与其他鱼类对应的基因聚为一支。氨基酸同源性分析显示，半滑舌鳎的pmelb与大菱鲆和牙鲆的相似性最高(分别为68.05%和66.28%)。分别通过分析半滑舌鳎lnc-XR＿003049606.1和pmelb的表达分析发现，lnc-XR＿003049606.1在1龄半滑舌鳎的皮肤和肝脏中表达，皮肤中表达量在60 d达到顶峰，随着时间延长而降低；而pmelb则主要在皮肤中表达，表达量在时间线上先升高再降低。本研究初步探究了lnc-XR＿003049606.1和pmelb间的表达关系，为进一步筛选半滑舌鳎无眼侧黑化过程中起作用的关键基因提供了思路。

关键词：半滑舌鳎 lncRNA pmelb 体色黑化

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碳限额政策约束下基于收益共享契约的两级供应链协调

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物流技术 2015年第17期34卷 216-218,226页

作者：王东晖李阳珍罗振宇西南财经大学天府学院四川成都610052 西南民族大学四川成都610041

研究了在碳限额为外部限制条件下的两级供应链协调问题。通过对供应链共享契约的合理设计,实现在碳限额政策下的两级供应链协调,实现企业间的利润共享、风险共担,提升供应链整体利益。通过对上述问题的研究,得到了如下结论:在考虑收益... 详细信息

研究了在碳限额为外部限制条件下的两级供应链协调问题。通过对供应链共享契约的合理设计,实现在碳限额政策下的两级供应链协调,实现企业间的利润共享、风险共担,提升供应链整体利益。通过对上述问题的研究,得到了如下结论:在考虑收益共享契约的两级供应链协调下,一定存在一个比成本更低的批发价,此时,生产商的利润全部来自于销售商的收益共享中;由于政府碳限额的存在,生产商在碳限额条件下的产品最优供应量小于在非约束条件下的最优供应量;在碳限额情形下,收益共享契约当中的最优批发价格高于无限额情形下的最优批发价格的同时,零售商在此情形下会增加收益的保留比例来维持自己的收入水平。

关键词：碳限额收益共享供应链协调

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Photo‑Energized MoS_(2)/CNT Cathode for High‑Performance li–CO_(2)Batteries in a Wide‑Temperature Range

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Nano-Micro Letters 2025年第1期17卷 160-175页

作者： Tingsong Hu Wenyi lian Kang Hu Qiuju li Xueliang Cui Tengyu Yao Laifa Shen Jiangsu Key Laboratory of Materials and Technologies for Energy Storage College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

li–CO_(2) batteries are considered promising energy storage systems in extreme environments such as Mars;however,severe performance degradation will occur at a subzero temperature owning to the sluggish reaction ***,a photo-energized strategy adopting sustainable solar energy in wide working temperature range li–CO_(2) battery was achieved with a binder-free MoS_(2)/carbon nanotube(CNT)photo-electrode as *** unique layered structure and excellent photoelectric properties of MoS_(2) facilitate the abundant generation and rapid transfer of photo-excited carriers,which accelerate the CO_(2) reduction and li_(2)CO_(3) decomposition upon *** illuminated battery at room temperature exhibited high discharge voltage of 2.95 V and mitigated charge voltage of 3.27 V,attaining superior energy efficiency of 90.2%and excellent cycling stability of over 120 *** at an extremely low temperature of−30℃,the battery with same electrolyte can still deliver a small polarization of 0.45 V by the photoelectric and photothermal synergistic mechanism of MoS_(2)/CNT *** work demonstrates the promising potential of the photo-energized wide working temperature range li–CO_(2) battery in addressing the obstacle of charge overpotential and energy efficiency.

关键词： li-CO_(2)batteries Photo-energized Wide operation-temperature Kinetics MoS_(2)

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He li li Sai Wang Shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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