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Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium

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Frontiers of Medicine 2016年第3期10卷 330-335页

作者： Yan Chen wenyan li Wen li Renjie Chai Huawei li Otorhinolaryngology Department of Affiliated Eye and ENT Hospital State Key Laboratory of Medical Neurobiology Fudan University Shanghai 200031 China Central Laboratory Affiliated Eye and ENT Hospital Fudan University Shanghai 200031 China Key Laboratory of Hearing Medicine of the National Health and Family Planning Commission Shanghai 200031 China MOE Key Laboratory of Developmental Genes and Human Disease State Key Laboratory of Bioelectronies Institute of Life Sciences Southeast University Nanjing 210096 China Co-innovation Center of Neuroregeneration Nantong University Nantong 226001 China Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

The enhancer of zeste 2 polycomb repressive complex 2 subunit （Ezh2） is a histone-lysine N- methyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 （E9.5）. At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day （P） 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.

关键词： polycomb repressive complex Ezh2 expression inner ear cochlea development

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Regeneration of hair cells in the mammalian vestibular system

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Frontiers of Medicine 2016年第2期10卷 143-151页

作者： wenyan li Dan You Yan Chen Renjie Chai Huawei li Otorhinolaryngology Department Affiliated Eye and ENT Hospital State Key Laboratory of Medical Neurobiology Fudan University Shanghai 200031 China Key Laboratory of Hearing Medicine of the National Health and Family Planning Commission Shanghai 200031 China Research Center Affiliated Eye and ENT Hospital Fudan University Shanghai 200031 China MOE Key Laboratory of Developmental Genes and Human Disease State Key Laboratory of Bioelectronics Institute of Life Sciences Southeast University Nanjing 210096 China Co-Innovation Center of Neuroregeneration Nantong University Nantong 226001 China Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

Hair cells regenerate throughout the lifetime of non-mammalian vertebrates, allowing these animals to recover from hearing and balance deficits. Such regeneration does not occur efficiently in humans and other mammals. Thus, balance deficits become permanent and is a common sensory disorder all over the world. Since Forge and Warchol discovered the limited spontaneous regeneration of vestibular hair cells after gentamicin- induced damage in mature mammals, significant efforts have been exerted to trace the origin of the limited vestibular regeneration in mammals after hair cell loss. Moreover, recently many strategies have been developed to promote the hair cell regeneration and subsequent functional recovery of the vestibular system, including manipulating the Wnt, Notch and Atohl. This article provides an overview of the recent advances in hair cell regeneration in mammalian vestibular epithelia. Furthermore, this review highlights the current limitations of hair cell regeneration and provides the possible solutions to regenerate functional hair cells and to partially restore vestibular function.

关键词： uncle hair cell regeneration Atohl Notch Wnt

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Preparation of SiC powders by carbothermal reduction with bamboo charcoal as renewable carbon source

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Journal of Advanced Ceramics 2013年第2期2卷 128-134页

作者： Xingzhong GUO lin ZHU wenyan li Hui YANG Department of Materials Science and Engineering Zhejiang UniversityHangzhou 310027China

Bamboo charcoal was expected to be a renewable carbon source for carbide materials in carbothermal reduction because of its superior *** powders with characteristic shapes were fabricated by carbothermal reduction with industrial silica sol and bamboo charcoal particles as silicon and carbon sources respectively,and the effects of reacting temperature and time on shape evolutions and properties of the as-prepared SiC powders were *** silica sol/bamboo charcoal system was firstly transformed into SiO_(2)/C system by the transition of silica sol and graphitization of bamboo charcoal,and the carbothermal reduction between SiO_(2) and C occurred at/above 1600℃.The characteristic shapes of SiC particles were transformed from string-beads-like to dumbbell-like and rod-like with the increase of reacting *** prepared SiC powders are expected to become new raw material for silicon carbide ceramic composites.

关键词： carbides chemical synthesis X-ray diffraction(XRD) microstructure

Numerical simulation and experimental verification of chemical reactions for SCR DeNOx

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Frontiers of Chemical Science and Engineering 2010年第4期4卷 523-528页

作者： Qiang ZHANG Yonglin FAN wenyan li Xi’an Thermal Power Research Institute Co.Ltd. Xi’an 710032China North China Electric Power University Beijing 102206China

Selective catalytic reduction(SCR)is a major commercial technology for NOx removal in power *** are a lot of complex chemical reactions in SCR reactors,and it is of great significance to understand the internal process of chemical reactions for SCR DeNOx and study the impact of various factors on NOx removal *** this paper,the impact of reaction temperature,ammonia-nitrogen molar ratio and resident time in the catalyst bed layer on NOx removal efficiency were studied by simulation of chemical *** calculated results were compared with catalyst activity test data in a power plant,which proved that the simulated results were *** a result,the reaction conditions were optimized in order to get the best removal efficiency of NO,so that we can provide a reference for optimal running of SCR in power plants.

关键词： SCR NOx removal efficiency chemical reactions simulation

Preparation of MnO2 Loaded Hydrothermal Carbon-coated Electrospun PAN Fiber Membranes for Highly Efficient Adsorption and Separation of Cationic Dye

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Chemical Research in Chinese Universities 2020年第6期36卷 1292-1301页

作者： WANG Yuannan li wenyan CHAO Shen li Yanzi li Xiang HE Dayong WANG Ce Alan G.MacDiarmid Institute College of ChemistryJilin UniversityChangchun 130012P.R.China Teaching Experimental Center Hebei Normal University for NationalitiesChengde 067000P.R.China

Hydrothermal carbonaceous materials and MnO2 have been proved to be promising adsorbents to remove organic dyes from *** this study,flexible MnO2 loaded hydrothermal carbon-coated electrospun poly-acrylonitrile(AC/MnO/PAN)fiber membranes were fabricated by a facile one-step hydrothermal method and activated by NaOH *** composite fibers exhibited large adsorption capacity toward cationic dyes and ex-cellent mechanical *** adsorption performance can be ftted well with pseudo-second order model and Langmuir isotherm *** maximum adsorption for methylene blue(MB),methyI violet(MV)and malachite green(MG)are 1173.27,1106.31 and 1129.89 mg/g,respectively,according to Langmuir *** AC/MnO/PAN fiber membrane also showed satisfactory performances for selective adsorption and *** addition,based on selective adsorption,the AC/MnOz/PAN fiber membranes that are repulsive to the anionic dye methyl orange(MO)can separate the MB/MO mixture solution by dynamic ***,this work not only provides a facile strategy to fabricate large capacity adsorbents,but also dermonstrates the potential appications in the dye wastewater treatment field.

关键词： Carbon MnO One-step hydrothermal Electrospinning Adsorption Cationic dye

Unidirectional and stage-dependent roles of Notch1 in Wnt-responsive Lgr5+ cells during mouse inner ear development

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Frontiers of Medicine 2019年第6期13卷 705-712页

作者： Hui Jiang Shan Zeng Wenli Ni Yan Chen wenyan li ENT Institute and Otorhinolaryngology Department of Affiliated Eye and ENT Hospital Fudan UniversityShanghai 200031China Key Laboratory of Hearing Medicine of NHFPC Shanghai 200031China Otorhinolaryngology Department of Jinshan Hospital Fudan UniversityShanghai 201508China

Wnt and Notch signaling play crucial roles in the determination of the prosensory domain and in the differentiation of hair cells(HCs)and supporting cells during mouse inner ear development;however,the relationship between the two signaling pathways in the mouse cochlea remains largely ***,we investigated the interactions between Notch and Wnt signaling on the basis of the bidirectional regulation of Notch1 specifically in Wnt-responsive Lgr5+progenitors during different cochlear development *** found that the downregulation of Notch1 in Lgr5+cells from embryonic day(E)14.5 to E18.5 can drive the quiescent Lgr5+cells to re-enter the cell cycle and differentiate into extra HCs,whereas the upregulation of Notch1 expression did not affect the proliferation or differentiation of otic progenitor *** effect was observed on the upregulation or downregulation of Notch1 in Lgr5+cells from E10.5 to *** concluded that the roles of Notch1 in Wnt-responsive Lgr5+cells are unidirectional and stage dependent and Notch1 serves as a negative regulator for Lgr5+progenitor activation during cochlear *** findings improved the understanding of the interactions between Notch and Wnt signaling in cochlear development.

关键词： inner ear cochlear Wnt Notch Lgr5 auditory system

A Novel Hollow Carbon@MnO_(2) Electrospun Nanofiber Adsorbent for Efficient Removal of Pb^(2+) in Wastewater

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Chemical Research in Chinese Universities 2021年第3期37卷 496-504页

作者： li wenyan li Yanzi liU Jiadi CHAO Shen YANG Tianyi li lijuan WANG Ce li Xiang Alan G.MacDiarmid Institute Jilin UniversityChangchun130012China Teaching Experimental Center Hebei Normal University for NationalitiesChengde067000China College of Plant Science Jilin UniversityChangchun130062China Department of Chemistry and Biochemistry California State UniversityLong Beach1250 Bellflower Blvd.Long BeachCA90840USA

Lead ion(Pb^(2+))is one of the most hazardous heavy metal ions in aquatic *** materials and manganese dioxide(MnO_(2))have been shown to be prospective adsorbents to cope with the lead *** this study,a novel hollow carbon@MnO_(2) composite nanofiber adsorbent was prepared by the combination of electrospinning and *** PAN nanofiber membrane is subjected to a pre-oxidation and carbonization process,and then the obtained carbon nanofibers react with KMnO4 solution during the hydrothermal process to develop the hollow carbon@MnO_(2) *** hollow carbon@MnO_(2) nanofibers displayed a higher adsorption capacity of Pb^(2+)than carbon and MnO_(2)/PDA/PAN *** maximum adsorption capacity toward Pb^(2+)by hollow carbon@MnO_(2) nanofibers was 460.83 mg/*** 5 adsorption-desorption cycles,the carbon@MnO_(2) nanofibers had a good recyclability and the removal efficiency remained 81.47%.Moreover,the removal efficiency of the hollow composite nanofibers for Pb^(2+)from real wastewater could reach 94.37%.This work shows a strategy for synthetics of the hollow carbon@MnO_(2) nanofibers,which exhibits a promising potential in actual wastewater treatment.

关键词： Carbon@MnO_(2)composite Hollow nanofiber Electrospinning Lead adsorption

INDUSTRIAliZATION AND INDUSTRIAL ALLOCATION IN CHINA

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Journal of Geographical Sciences 1992年第2期 1-13页

作者： li wenyan Pang Xiaomin Institute of Geography CAS

The process of industrialization in China has been carried out under specific national circumstances,and it is rather different from that of other countries *** the past four decades,a great change of industrial develop?ment and industrial allocation has taken place in China,and among the reasons for such a change,the government strategy of industrialization is the chief *** phrasal industrial change in China concerns the dynamic sectoral and spatial industrial structure,especially the formation of a specific industrial system with several levels.

关键词： industrialization strategy industrial allocation

Dynamic responses ofPAto environmental stimuli imaged by a genetically encoded mobilizable fluorescent sensor

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Plant Communications 2023年第3期4卷 99-111页

作者： Teng li Xingkai Xiao Qingyun liu wenyan li li li Wenhua Zhang Teun Munnik Xuemin Wang Qun Zhang College of life Sciences State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing 210095China Institute of Botany Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Nanjing 210014China Cluster Green life Sciences Section Plant Cell BiologySwammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamthe Netherlands Department of Biology University of Missouri-St.LouisSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Membrane fluidity,permeability,and surface charges are controlled by phospholipid metabolism and *** the importance of phosphatidic acid(PA)as a bioactive molecule,the mechanical properties of PA translocation and subcellular accumulation are ***,we used a mobilizable,highly responsive genetically encoded fluorescent indicator,green fluorescent protein(GFP)-N160RbohD,to monitor PA dynamics in living *** majority of GFP-N160RbohD accumulated at the plasma membrane and sensitively responded to changes in PA ***,pharmacological,and genetic analyses illustrated that both salinity and abscisic acid rapidly enhanced GFP-N160RbohD fluorescence at the plasma membrane,which mainly depended on hydrolysis of phospholipase *** contrast,heat stress induced nuclear translocation of PA indicated by GFP-N160RbohD through a process that required diacylglycerol kinase activity,as well as secretory and endocytic ***,we showed that gravity triggers asymmetric PA distribution at the root apex,a response that is suppressed by PLDz2 *** broad utility of the PA sensor will expand our mechanistic understanding of numerous lipid-associated physiological and cell biological processes and facilitate screening for protein candidates that the synthesis,transport,and metabolism of PA.

关键词： phospholipid phosphatidic acid RbohD heat stress salinity abscisic acid