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Acta Oceanologica Sinica 2011年第6期30卷 86-94页

作者： ZHANG Dajuan li shaojing WANG Guizhong GUO Donghui College of Oceanography and Environmental Science Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

Ecological experiments were conducted to examine the effects of seawater containing elevated par- tial pressure of carbon dioxide （p CO2 800 × 10 -6 , 2 000 × 10 -6 , 5 000 × 10 -6 and 10 000 × 10 -6 ） on the survival and reproduction of female Acartia pacifica, Acartia spinicauda, Calanus sinicus and Centropages tenuiremis, which are the dominant copepods in the southern coastal waters of China. The results show that the effects of elevated p CO2 on the survival rates of copepods were species-specific. C. sinicus, which was a macro-copepod, had a higher survival rate （62.01%–71.96%） than the other three species （5.00%–26.67%） during the eight day exposure. The egg production rates of C. sinicus, A. spinicauda and C. tenuiremis were significantly inhibited by the increased p CO2 and the exposure time duration. There were significantly negative impacts on the egg hatching success of A. spinicauda and C. tenuiremis in the p CO2 2 000 × 10 -6 and 10 000 × 10 -6 groups, and, in addition, the exposure time had noticeably impacts on these rates too. This study indicates that the reproductive performances of copepods were sensitive to elevated p CO2 , and that the response of different copepod species to acidified seawater was different. Furthermore, the synergistic effects of seawater acidification and climate change or other pollutant stresses on organisms should be given more attention.

关键词： CO2-driven acidification copepod survival reproductive performance

Occurrence of gonadtropins like substance in the thoracic ganglion mass of the mud crab, Scylla paramamosain (Crustacea: Decapoda: Brachyura)

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Acta Oceanologica Sinica 2009年第5期28卷 76-80页

作者： YE Haihui HUANG Huiyang WANG Guizhong li shaojing Department of Oceanography ＆ Institute of Subtropical Oceanograpy Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

The identification and localization of vertebrate follicle stimulating hormone （FSH） and luteinizing hormone （LH） in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using immunocytochemical staining techniques, this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass （TGM） of Scylla paramamosain （Crustacea： Decapoda： Brachyura）. Enzyme-linked immunosorbent assay （EliSA） has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage. These results indicate that substances resembling the vertebrate FSH and LH are present in S. paramamosain, and they may be involved in the development of the ovary as well as in ovulation.

关键词： follicle stimulating hormone (FSH) leuteinizing hormone (LH) Scylla paramamosain thoracic ganglion mass immunocytochemistry enzyme-linked immunosorbent assay (EliSA)

Cloning and expression of aequorin genes from jellyfish Aequorea and characterization of aequorins activities

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Acta Oceanologica Sinica 2002年第4期21卷 547-556页

作者： Luo Wenxin Zhang Jun li Shaowei Cheng Tong Chen Min li shaojing Xia Ningshao Cell Biology and Tumor Cell Engineering Laboratory Ministry of EducationXiamen UniversityXiarnen 361005China

Two new aequorin genes,aeqxm and aeqxxm,were isolated from jellyfish Aequorea macrodactyla and Aequorea parva respectively,which are commonly found in the warmer waters on the coastal region of the East China *** DNA sequences of the two genes have no introns and each one contains an ORF of 585 bp in full-length encoding a 195-aa *** two genes of aeqxm and aeqxxm share nucleotide homologies of 80.7%and 85.1%with AEVAQ440X respectively,and the corresponding proteins share amino acid homologies of 84.7%and 84.2%with *** amino acid homology was found between apoaeqxm and *** two genes were cloned into expression vector pTO-T7 respectively,and the expression yields amounted to 40%of the total protein in *** *** activities of the two photoproteins were reconstituted by incubating the expressed apoproteins with coelenterazine *** the presence of Ca ion,both of the regenerated aeqxm and aeqxxm exhibited an emission peak at the wave length of 470 nm.

关键词： Aequorea macrodactyla Aequorea parva aequorin expression

On Two New Species of Planktonic Copepoda from the Estuary of Jiulong River, Fujian, China

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厦门大学学报(自然科学版) 1984年第3期 385-390页

作者： li shaojing Xuang Jiaqhi (Department of Oceanography)

The diagnoses of new species, the types of which are deposited in theDepartment of Oceanography, Xiamen University, are given as follows:1. Bestiola amoyensis sp. nov. (Figs. 1--10)Type specimens: Holotype, female, XO... 详细信息

关键词： Fujian China

Genetic diversity and differentiation of mud crab Scylla serrata populations from southeastern China

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Acta Oceanologica Sinica 2004年第2期23卷 309-316页

作者： liZhongbao lishaojing WANGGuizhong FisheriesCollege JimeiUniversityXiamen361021China SchoolofMarineandEnvironmentalStudies XiamenUniversityXiamen361005China

The genetic diversity and differentiation of 6 mud crab Scylla serrata populations from southeastern China are investigated using allozyme electrophoresis. The mean number of alleles per locus population is 1.3 ± 0.1; the percentage of polymorphic loci per population is 27.3; the observed heterozygosity ranges from (0.195±0.083) to ( 0.241±0.090) and the expected heterozygosity ranges from ( 0.105±0.043 ) to ( 0.131±0.047 ). The coefficient of gene differentiation among populations is low (Fst =0.032), indicating that only 3.2 % of the total genetic diversity comes from inter-population, while the remaining 96.8 % comes from intra-population differences. The genetic distance among populations is 0.000-0.008 (the average is 0.002). Gene flow among the populations is large (Nm = 7.56).Genetic structure is very similar among 6 Scylla serrata populations.

关键词： Scylla serrata allozyme genetic diversity genetic structure genetic differentiation

A preliminary study of the variation of phytoplankton absorption coefficients in the northern South China Sea

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Acta Oceanologica Sinica 2009年第5期28卷 17-29页

作者： WU Jingyu HONG Huasheng SHANG Shaoling DAI Minhan LEE Zhongping li shaojing State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China Department of Oceanography Xiamen University Xiamen 361005 China Naval Research Lab Code 7333 Stennis Space Center MS 39529 USA

The temporal and spatial variabilities of phytoplankton absorption coefficients （a ph （λ）） and their relationships with physical processes in the northern South China Sea were examined, based on in situ data collected from two cruise surveys during May 14 to 25, 2001 and November 2 to 21, 2002. Significant changes in the surface water in a ph values and B/R ratios （a ph （440）/a ph （675）） were observed in May, which were caused by a phytoplankton bloom on the inner shelf stimulated by a large river plume due to heavy precipitation. This is consistent with the observed one order of magnitude elevation of chlorophyll a and a shift from a pico/nano dominated phytoplankton community to one dominated by micro-algae. Enhanced vertical mixing due to strengthened northeast monsoon in November has been observed to result in higher surface a ph （675）（0.002–0.006 m-1 higher） and less pronounced subsurface maximum on the outer shelf/slope in November as compared with that in May. Measurements of a ph and B/R ratios from three transects in November revealed a highest surface a ph （675） immediately outside the mouth of the Zhujiang （Pearl） River Estuary, whereas lower a ph （675） and higher B/R ratios were featured in the outer shelf/slope waters, demonstrating the respective influence of the Zhujiang River plume and the oligotrophic water of the South China Sea. The difference in spectral shapes of phytoplankton absorption （measured by B/R ratios and bathochromic shifts） on these three transects infers that picoprocaryotes are the major component of the phytoplankton community on the outer shelf/slope rather than on the inner shelf. A regional tuning of the phytoplankton absorption spectral model （Carder et al., 1999） was attempted, demonstrating a greater spatial variation than temporal variation in the lead parameter a 0 （λ）. It was thus implicated that region-based parameterization of ocean color remote sensing algorithms in the northern South China Sea was mandatory.

关键词： phytoplankton absorption coefficient variation picoprokaryotes parameterization South China Sea

Chemical induction of settlement and metamorphosis in two Babylonia (Gastropoda) larvae

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Acta Oceanologica Sinica 2000年第1期19卷 59-67页

作者： Ke Caihuan li shaojing li Fuxue Zheng Zhon liu Weibin (1. Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China 2. Fujian Fisheries Research Institute, Xiamen 361012, China) Department of Oceanography Inst. of Subtropical Oceanography Xiamen University Xiamen 361005 China Fujian Fisheries Research Institute Xiamen 361012 China

By exposure to 12 x 10 3 mol/dm3 K+ for 24 h, more than 90% of planktonic larvae of Babylonia formosae and B. areolata were effectively induced to metamorphose completely. 15 x 10 - 3 mol/dm3 K+ exerted toxic effect on larval B. areolata over a 24-h period, but produced better results of induction by 12 h exposure. Metamorphosed juveniles induced by increased by K' could move and feed normally. Percent metamorphosis increased with average shell height for K + -induced larvae. L-DOPA, epinephrine and norepinephrine ranging from 5. 5 x 10 -- 6 to 5. 5 x 10 - 5 mol/dm3 had no inductive activity to metamorphosis in larval B. areolata, whereas the rate of metamorphosis induced by dopamine at a concentration of 10--4 mol/dm3 was approximately half less than that by 12 x 10 3 mol/dm3 K+. TEA (a K+ -channel blocker) did not inhibit metamorphosis in the two Babylonia larvae by induction of K+. Based on the results of K+ -induced experiments, it is preliminarily suggested that shell height of larvae achieving metamorphic competence was in the range of 860. 2 - 1 009. 8μm for B. formosae, and 849. 6- 956. 0 um for B. areolata. In addition, planktonic larvae of the two Babylonia had delayed metamorphosis.

关键词： Chemical induction metamorphosis Babylonia formosae B. areolata larva

Distribution characteristics of zooplankton in the south part of Taiwan Strait in summer

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Acta Oceanologica Sinica 2000年第1期19卷 127-135页

作者： Zhu Changshou Huang Jiaqi lin Junzhou Du Qinghong Chen Xu li shaojing (1. Fujian Institute of Oceanology, Xiamen 361012, China 2. Department of Oceanography, Xiamen University, Xiamen 361005, China) Fujian Institute of Oceanology Xiamen 361012 China Department of Oceanography Xiamen University Xiamen 361005 China

Based on the samples collected mesoplankton net from the south part Of Taiwan Strait (22. 25 --24. 65N, 117. 50 -- 120. 00E) in August 1997, 292 species of zooplankton and 11 groups of meroplankton were identified. In the survey waters, Copepoda was dominant in either species or individual numbers. The diversity (H' ) was 5. 11 and the ratio of diversity to evenness (J ) was 7. 10, showing that the diversity of zooplankton was exceedingly high in the survey waters. Three zooplankton communities were clearly divided by systematical cluster analysis, indicating that the biotopes of these zooplankton communities may serve as the evidence for the basic status of distribution of water masses during the investigation.

关键词： Zooplankton species diversity distribution characteristics Taiwan Strait

Structures of ovary and ovarian follicle in flathead lobster,Thenus orientalis (Lund, 1793) (Crustacea: Decapoda: Scyllarida)

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Acta Oceanologica Sinica 2001年第2期20卷 241-250页

作者： Zhu Dongfa, li shaojing, Wang Guizhong (1. Department of Oceanography, Xiamen University, Xiamen 361005, China 2. Faculty of life Science and Biotechnology, Ningbo University, Ningbo 315211, China) Department of Oceanography Xiamen University Faculty of Life Sci./Biotechnology Xiamen 361005 China

The ovary contains four morphological components: (1) the ovarian wall, (2) the reproductive epithelium, (3) the cellular layer containing oocytes, oogonia (especially for early-developing ovary) and follicle cells, and (4) the extensions of the ovarian wall. The ovarian wall and its extensions consist of blood vessels, sinuses, muscle cells and others. The extensions of the ovarian wall project into among the follicles and insert on the thick basal membrane of each follicle. From inside to outside, the follicles are composed of four parts: (1) the oocyte, (2) the perivitelline space, (3) the follicle cells, and (4) the basal membrane. The surface of the oocyte during vitellogenesis is folded into numerous long microvilli that project into the perivitelline space between the oocyte surface and the bace of the follicle cell layer. In addition, the plasma membrane of the vitellogenic oocyte contains many pinocytotic pits. The perivitelline space is engorged with with more electron- denser material as the development of the follicle. The inclusion of perivitelline space in the mature follicle is named specially as the chorine. The chorion is composed of two region, a thinner exochorion and a thicker endochorion containing electron-dense granular material. The follicle cell layer is composed of a single layer of polygonal follicle cells which exhibit higher synthetic activity. The synthetic product of the follicle cell layer is one scarce for the inclusion of the perivitelline space. The structures of the ovary and ovarian follicle in T. orientalis show that the exogenously biosyn- thetic yolk plays important roles in the vitellogenesis.

关键词： Thenus orientalis ovary follicle chorion formation

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己烯雌酚影响锯缘青蟹幼蟹生长的初探

厦门大学学报（自然科学版） 1989年第2期28卷 199-202页

作者：王桂忠李少菁厦门大学海洋学系

研讨了■烯雌酚对锯缘青蟹幼蟹的存活,摄食和生长蜕壳的影响。结果表明:■■■■量(2μg/g■d)的已烯雌酚能促进幼蟹的蜕壳,提高日摄食量,但仅注身已烯雌酚■■■幼蟹的■,■低摄食量、且存活率随着剂量加大和注射间隔时间的缩短而下的... 详细信息

研讨了■烯雌酚对锯缘青蟹幼蟹的存活,摄食和生长蜕壳的影响。结果表明:■■■■量(2μg/g■d)的已烯雌酚能促进幼蟹的蜕壳,提高日摄食量,但仅注身已烯雌酚■■■幼蟹的■,■低摄食量、且存活率随着剂量加大和注射间隔时间的缩短而下的。■■■■克含有14和18μg已烯雌酚的饵料对幼蟹的生长有明显的促进作用。

关键词：锯缘青蟹己烯雌酚幼蟹蜕壳