检索结果-南通市图书馆

Neuroscience Bulletin 2018年第1期34卷 165-177页

作者： li min chen Departments of Radiology and Radiological Sciences and Psychology Institute of Imaging Science Vanderbilt University Medical Center

Human functional MRI studies in acute and various chronic pain conditions have revolutionized how we view pain, and have led to a new theory that complex multi-dimensional pain experience （sensory-discriminative, affective/motivational, and cognitive） is represented by concurrent activity in widely-distributed brain regions （termed a network or pain matrix）. Despite these break- through discoveries, the specific functions proposed for these regions remain elusive, because detailed electrophys- iological characterizations of these regions in the primate brain are lacking. To fill in this knowledge gap, we have studied the cortical areas around the central and lateral sulci of the non-human primate brain with combined submillimeter resolution functional imaging （optical imaging and fMRI） and intracranial electrophysiological recording. In this mini-review, I summarize and present data showing that the cortical circuitry engaged in nociceptive processing is much more complex than previously recognized. Electrophysiological evidence supports the engage- ment of a distinct nociceptive-processing network within SI （i.e., areas 3a, 3b, 1 and 2）, SII, and other areas along the lateral sulcus. Deafferentation caused by spinal cord injury profoundly alters the relationships between fMRI and electrophysiological signals. This finding has significant implications for using fMRI to study chronic pain conditions involving deafferentation in humans.

关键词： Nociception Non-human primate Cortex Functional MRI Functional connectivity

Health Effects of Airborne Particulate Matter Trace Elements

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2005年第5期18卷 349-355页

作者： XIANG GAO QI YU li-min chen Department of Environmental Science and Engineering Fudan University Shanghai 200433 China

The effects of airborne particulate matter （PM） trace elements on health are widely concerned nowadays. Many achievements have been made while many unknowns exist. This article reports the recent research progresses, describes the effects of exposure to PM trace elements on health epidemiological evidence, toxicology findings, and raises some questions for future studies.

关键词： Health effect Particulate matter Trace element

Flavonoid triglycosides from the seeds of Camellia oleifera Abel

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2008年第11期19卷 1315-1318页

作者： li Chun Du Bei li Wu Jian min chen Department of Environmental Science ＆ Engineering Fudan University Shanghai 200433 China

Two flavonoid triglycosides, kaempferol 3-O-{ β-D-glucopyranosyl-（1→2）-[α-L-rhamnopyranosyl-（1→ 6）]-β-D-glucopyranoside} （1） and kaempferol 3-O-{ β-D-xylopyranosyl-（1 → 2）-[α-L-rhamnopyranosyl-（1→6）]-β-D-glucopyranoside } （2）, were isolated from the seed of Camellia oleifera Abel. The absolute configuration of compound 1 was established on the basis of its X-ray analysis. Their cytotoxic activities and anti-HIV-RT activities were evaluated.

关键词： Flavonoid triglycosides Camellia oleifera Abel Cytotoxic activity

Differentiation of human umbilical cord blood stem cells into hepatocytes in vivo and in vitro

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第25期12卷 4014-4019页

作者： Xiao-Peng Tang min Zhang Xu Yang li-min chen Yang Zeng Research Center of liver Diseases the Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China

AIM： To study the condition and potentiality of human umbilical cord blood stem cells （HUCBSC） to differentiate into hepatocytes in vivo or in vitro. METHODS： In a cell culture study of human umbilical cord blood stem cell （HUCBSC） differentiation, human umbilical cord blood mononuclear cells （HUCBMNC） were separated by density gradient centrifugation. Fibroblast growth factor （FGF） and hepatocyte growth factor （HGF） and the supernatant of fetal liver were added in the inducing groups. Only FGF was added in the control group. The expansion and differentiation of HUCBMNC in each group were observed. Human alpha fetoprotein （AFP） and albumin （ALB） were detected by immunohistochemistry. In the animal experiments, the survival SD rats with acute hepatic injury after carbon tetrachloride （CCL4） injection 48 h were randomly divided into three groups. The rats in group A were treated with human umbilical cord blood serum. The rats in group B were treated with HUCBMNC transplantation. The rats in group C were treated with HUCBMNC transplantation followed by intraperitoneal cyclophosphamide for 7 d. The rats were killed at different time points after the treatment and the liver tissue was histopathologically studied and human AFP and ALB detected by immunohistochemistry. The human X inactive-specific transcript gene fragment in the liver tissue was amplified by PCR to find human DNA. RESULTS： The results of cell culture showed that adherent cells were stained negative for AFP or ALB in control group. However, the adherent cells in the inducing groups stained positive for AFP or ALB. The result of animal experiment showed that no human AFP or ALB positive cells present in the liver tissue of group A （control group）. However, many human AFP or ALB positive cells were scattered around sinus hepaUcus and the central veins of hepatic Iobules and in the portal area in group B and group C after one month. The fragment of human X chromagene could be detected in the liver tissue

关键词： Umbilical cord blood Stem cell liver failure Cell transplantation Cell differentiation

Effects of deoxidizing degree on the pitting corrosion behavior of carbon and manganese steels

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of minerals,Metallurgy and Materials 2011年第2期18卷 169-177页

作者： Guo-liang Cao Cuo-min li Shan chen Wan-shun Chang Xue-qun chen College of Sciences Naval University of Engineering Wuhan 430033 China

Nine steels with different deoxidizing degrees and two comparative steels were selected. Their pitting initiation susceptibility was compared by means of potentiodynamic polarization tests in 3wt% NaCl solution. The pit propagation rate was evaluated in artificial sea water and 3wt% sea salt solution by simulating occluded corrosion cell （SOCC） test and hanging plate test, respectively. The composition of inclusions and corrosive feature were studied by scanning electron microscopy （SEM）, electron probe micro-analysis （EPMA）, and optical microscopy （OM）. The results indicate that sulfide inclusions in steel are the sites for pit nucleation. The sulphide inclusions vary in shape from short spindle-like to long strip-like with increasing deoxidizing degree. Under the same conditions, the lower the deoxidizing degree gets, the lower the pitting initiation susceptibility becomes, and the stronger the resistance to pit propagation exhibits. For steels with different deoxidizing degrees, their pitting initiation susceptibility is mainly influenced by thermodynamic stability, while the pit propagation rate is primarily subject to the characteristics of inclusions in steel.

关键词： carbon steel manganese steel pitting deoxidization inclusions

Analysis on the time-domain characteristics of botnets control traffic

在线全文

学校读者我要写书评

暂无评论

The Journal of China Universities of Posts and Telecommunications 2011年第2期18卷 106-113页

作者： li Wei-min MIAO chen liU Fang LEI Zhen-ming School of Information and Communication Engineering Beijing University of Posts and Telecommunications Beijing 100876 China

Botnets are networks composed with malware-infect ed *** are designed and organized to be controlled by an *** victims are infected through their inappropriate network behaviors in most cases,the Internet protocol（IP） addresses of infected bots are ***,a bot can get an IP address through dynamic host configuration protocol（DHCP）,so they need to get in touch with the controller initiatively and they should attempt continuously because a controller can＇t be always *** whole process is carried out under the command and control（C＆C） *** goal is to characterize the network traffic under the C＆C channel on the time *** analysis draws upon massive data obtained from honeynet and a large Internet service provider（ISP） *** extract and summarize fingerprints of the bots collected in our ***,with the fingerprints,we use deep packet inspection（DPI） Technology to search active bots and controllers in the ***,we gather and analyze flow records reported from network traffic monitoring *** this paper,we propose a flow record interval analysis on the time domain characteristics of botnets control traffic,and we propose the algorithm to identify the communications in the C＆C channel based on our *** that,we evaluate our approach with a 3.4 GB flow record trace and the result is *** addition,we believe that our work is also useful information in the design of botnet detection schemes with the deep flow inspection（DFI） technology.

关键词： botnet detection netflow record time domain analysis deep flow inspection

Oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第31期13卷 4260-4263页

作者： Yong-Zhong Wang Guo-Xiang Wu li-Bo Luo min chen li-Hua Ruan Research Center for liver diseases The ThirdHospital of Changzhou Changzhou 213001 Jiangsu ProvinceChina

To compare the oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus in Chinese patients with chronic hepatitis B. METHODS： Mixture of samples with different genotypes and clinical serum samples from 126 chronic hepatitis B patients was tested for hepatitis B virus genotypes by oligonucleotide chip, real-time PCR and sequencing of PCR products, respectively. Clinical performances, time required and costs of the three assays were evaluated. RESULTS： Oligonucleotide chips and real-time PCR detected 1% and 0.1% genotypes, respectively, in mixed samples. Of the 126 clinical samples from patients with chronic hepatitis B, genotype B was detected in 41 （33%）, 41 （33%） and 45 （36%） samples, and genotype C in 76 （60%）, 76 （60%） and 81 （64%） samples, by oligonucleotide chip, real-time PCR and sequencing, respectively. Oligonucleotide chip and real-time PCR detected mixed genotypes B and C in 9 samples. Real- time PCR was the rapidest and cheapest among the three assays. CONCLUSION： Oligonucleotide chip and real-time PCR are able to detect mixed genotypes, while sequencing only detects the dominant genotype in clinical samples.

关键词： Hepatitis B virus Genotypes Oligonucleotidechips Real-time PCR Sequencing

Health Management of Breast Cancer Survivors

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinical oncology and cancer researeh 2009年第3期6卷 174-178页

作者： min li Juan chen Zhendong chen Department of Oncology the First AffiliatedHospital of Anhui Medical University Hefei20022 Anhui Province China

Breast cancer is defined as a chronic *** amounts of attention have been paid to the healthmanagement of breast cancer survivors. An important issue ishow to find the most appropriate method of follow-up in orderto detect long-term complications of treatment, local recurrenceand distant metastasis and to administer appropriate treatmentto the survivors with recurrence in a timely fashion. Differentoncology organizations have published guidelines for followingup breast cancer survivors. However, there are few articles onthis issue in China. Using the published follow-up guidelines,we analyzed their main limitations and discussed the content,follow-up interval and economic benefits of following up breastcancer survivors in an effort to provide suggestions to *** on a large number of clinical trials, we discussed the roleof physical examination, mammography, liver echograph, chestradiography, bone scan and so on. We evaluated the effects ofthe above factors on detection of distant disease, survival time,improvement in quality of life and time to diagnosis of *** results of follow-up carried out by oncologists and primaryhealth care physicians were compared. We also analyzed thecorrelation factors for the cost of such follow-up. It appears thatfollow-up for breast cancer survivors can be carried out effectivelyby trained primary health care physicians. If anything unusualarises, the patients should be transferred to specialists.

关键词： breast cancer survivor health management.

Optical imaging of nociception in primary somatosensory cortex of nonhuman primates

在线全文

学校读者我要写书评

暂无评论

生理学报 2008年第5期60卷 664-668页

作者： li-min chen Robert M. Friedman Anna W. Roe Departments of Radiology and Radiological Science Vanderbilt University Nashville TN 3 7232 USA Departments of psychology Vanderbilt University Nashville TN 3 7232 USA Departments of Institute of lmaging Science Vanderbilt University Nashville TN 3 7232 USA

While the activation of primary somatosensory(SI) cortex during pain perception is consistently reported in functional imaging studies on normal subjects and chronic pain patients,the specific roles of SI,particularly the subregions within SI,in the processing of sensory aspects of pain are still largely *** optical imaging of intrinsic signal(OIS) and single unit electrophysiology,we studied cortical activation patterns within SI cortex(among Brodmann areas 3a,3b and 1) and signal amplitude changes to various intensities of non-nociceptive,thermal nociceptive and mechanical nociceptive stimulation of individual distal finerpads in anesthetized squirrel *** have demonstrated that areas 3a and 1 are preferentially involved in the processing of nociceptive information while areas 3b and 1 are preferentially activated in the processing of non-nociceptive(touch) *** activations of individual fingerpad were organized topographically suggesting that nociceptive topographic map exits in areas 3a and *** amplitude was enhanced to increasing intensity of mechanical nociceptive stimuli in areas 3a,3b and *** area 1,nociceptive response co-localizes with the non-nociceptive ***,we hypothesize that nocicepitve information is area-specifically represented within SI cortex,in which nociceptive inputs are preferentially represented in areas 3a and 1 while non-nociceptive inputs are preferentially represented in areas 3b and 1.

关键词：光学图象分析体感皮质疼痛分析猴类

DIAGNOSTIC VALUE OF INTERICTAL DIFFUSION-WEIGHTED IMAGING IN EVALUATION OF INTRACTABLE TEMPORAL LOBE EPILEPSY

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2008年第2期23卷 68-72页

作者： Rui Wang Sa-ying li min chen cheng Zhou Department of Radiology Beijing Hospital Beijing 100730

Objective To explore the ability of interictal diffusion-weighted imaging(DWI)to localize the temporal lobe of seizure origin and to predict postoperative seizure control in patients with temporal lobe epilepsy(TLE).Methods Twenty-seven patients with intractable TLE considered for surgery and 19 healthy volunteers were studied with conventional magnetic resonance imaging(MRI)and *** diffusion coefficients(ADCs)of bilateral hippocampi in both TLE patients and control subjects were *** to either temporal lobe with hippocampal ADC was based on the threshold values derived from ±1SD of right/left ratios in normal *** the postoperative pathology was *** Hippocampal ADCs were higher on the side of surgery compared with those on the contralateral side as well as the ipsilateral side in control subjects [resected side(109.8±7.3)×10-5 cm2/s,contralateral side(91.7±4.7)×10-5 cm2/s,control subjects(81.6±5.2)×10-5 cm2/s,all Plized to the operated temporal lobe in 21 of 27(77.8%)and in 18 of 27(66.7%)*** to the surgical side was not associated with postoperative seizure control with right/left hippocampal ADC ratio(P>0.05).Conclusions Conventional MRI is a sensitive method to detect hippocampal *** of the right/left hippocampal ADC ratio for lateralizing to the side of surgery is very high,but it isn't a better predictor of surgical outcome.

关键词： magnetic resonance imaging temporal lobe epilepsy epilepsy surgery