检索结果-南通市图书馆

When mRNA meets gene editing

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第8期17卷 7337-7356页

作者： Weijie li chen Wang {2. Lu Key Laboratory of Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China department of Chemical Engineering Tsinghua UniversityBeijing 100084China

The critical challenge of gene therapy lies in delivering gene editing *** with DNA,while RNA is less stable and more accessible to degrade,it comes with the benefit of lower off-target effects since permanent insertion is not *** review focuses on mRNA-based delivery of gene editing agents,highlighting novel mRNA delivery *** provide context,a comparison is made between three main gene editing agents:programmable nucleases,base editors,and prime *** potential of Cas\pi and transposons is also discussed in this ***,a summary of four main barriers to mRNAbased in vivo delivery is ***,this review detailedly introduced different delivery systems,both viral(lentivirus)and non-viral vectors(genome editing via oviductal nucleic acids delivery,lipid nanoparticles,polymer-based nanoparticles,viruslike-particles,extracellular vesicles,and migrasome).Each delivery strategy is assessed by comparing its advantages and disadvantages to offer a comprehensive and objective overview of the delivery ***,we emphasized the vital role of the protein corona as a critical regulator for ***,we concluded the challenges of mRNA-based gene editing strategies(RNA stability,targeting,potential immunogenicity,cytotoxicity,heterogeneity,and rational design).The purpose of this review is to guide further research and provide a comprehensive analysis of mRNA-based in vivo delivery of gene editing agents in this promising field.

关键词： gene editing mRNA delivery viral vectors non-viral vectors CRISPR

Resilience provides mediating effect of resilience between fear of progression and sleep quality in patients with hematological malignancies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2024年第4期14卷 541-552页

作者： yuan tian Ying-{2. Wang department of Hematology The First Affiliated Hospital of Jinzhou Medical UniversityJinzhou 121000Liaoning ProvinceChina department of Cardiology The First Affiliated Hospital of Jinzhou Medical UniversityJinzhou 121000Liaoning ProvinceChina

BACKGROUND Hematological tumors are common malignant tumors,with high morbidity and mortality *** patients with hematological malignancies develop sleep disorders that seriously affect their life and health because of acute onset of disease,rapid progression,high recurrence rates,complex treatment methods,and treatment *** To explore the mediating effect of resilience on fear of disease progression and sleep quality in patients with hematological *** A cross-sectional analysis of 100 patients with hematological malignancies,treated in the First Affiliated hospital of Jinzhou medical University between August 2.2. and August 2.2.,was *** were assessed using a general data survey,a simplified scale for the fear of progression(FoP)of disease,a resilience scale,and the Pittsburgh Sleep Quality *** analysis was conducted to determine the relationship between various patient characteristics and FoP,resilience,and sleep ***’s correlation analysis was used to examine the correlations between mental resilience,FoP,and sleep *** The total FoP score mean value in patients with hematological malignancies was 38.09±5.16;the total resilience score mean value was 40.73±7.04;and the Pittsburgh Sleep Quality Index score mean value was 10.72.***,resilience,and sleep quality of the patients were associated with family per capita monthly income and patient education level(Plience and sleep quality scores(r=-0.560,-0.537,Plience was significantly associated with sleep quality scores(r=0.688,Plience between FoP and sleep quality in patients with hematological malignancies was-0.100 and accounted for 50.51%of the total *** indicated that FoP directly and indirectly affected sleep quality through the mesomeric eff

关键词： Resilience Blood tumor Fear of progression Sleep quality Mediating effect

Seismic responses of an intensively constructed metro station-passageway-shaft structure system

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Structural and Civil Engineering 2024年第5期18卷 760-775页

作者： Ruohan li Yong yuan Hong {2. Xinxing li Emilio BILOTTA department of Geotechnical Engineering Tongji UniversityShanghai 200092China department of Civil Architectural and Environmental EngineeringUniversity of Naples Federico IINaples 80125Italy State Key Laboratory of Disaster Reduction in Civil Engineering Tongji UniversityShanghai 200092China Shanghai Tunnel Engineering&Rail Transit Design and Research Institute Shanghai 200235China

Intensive construction methods offer benefits for metro station development,yet they present challenges for seismic design due to the spatially asymmetric configuration of passageway-shaft *** this study,a detailed numerical model of a station-passageway-shaft structure system built using intensive construction methods was developed and the deformation and damage modes under seismic loadings were *** results indicate that inconsistent deformation between the shaft and the station generates interaction through the connecting passageway,leading to damage near the opening of the station structure and both ends of the connecting passageway Damage is more severe under longitudinal *** with the opening plan that spans four segments,the opening plan that spans five segments exacerbates the overall degree of damage to the structure *** transverse excitation,the presence of interior structures intensifies the damage to the station and connecting passageway,while with such internal structure in place the impact is relatively minor under longitudinal *** with steel segments near the station opening can appreciably attenuate the *** contrast,introducing flexible joints at both ends of the connecting passageway intensifies the ***,reinforcement using steel segments emerges as an optimal seismic mitigation strategy.

关键词： earthquake intensive construction metro station numerical simulation

Metarelation2.ec:A Metapath-Free Scalable Representation Learning Model for Heterogeneous Networks

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2024年第2期29卷 553-575页

作者： Lei chen yuan {2. Yong Lei Xingye Deng School of Information and Electrical Engineering Hunan University of Science and TechnologyXiangtan 411201China School of Computer Science and Engineering Hunan University of Science and TechnologyXiangtan 411201China

Metapaths with specific complex semantics are critical to learning diverse semantic and structural information of heterogeneous networks(HNs)for most of the existing representation learning ***,any metapaths consisting of multiple,simple metarelations must be driven by domain *** sensitive,expensive,and limited metapaths severely reduce the flexibility and scalability of the existing models.A metapath-free,scalable representation learning model,called Metarelation2.ec,is proposed for HNs with biased joint learning of all metarelations in a bid to address this ***,a metarelation-aware,biased walk strategy is first designed to obtain better training samples by using autogenerating cooperation probabilities for all metarelations rather than using expert-given ***,grouped nodes by the type,a common and shallow skip-gram model is used to separately learn structural proximity for each node ***,grouped links by the type,a novel and shallow model is used to separately learn the semantic proximity for each link ***,supervised by the cooperation probabilities of all meta-words,the biased training samples are thrown into the shallow models to jointly learn the structural and semantic information in the HNs,ensuring the accuracy and scalability of the *** experimental results on three tasks and four open datasets demonstrate the advantages of our proposed model.

关键词： metarelation random walk heterogeneous network metapath representation learning

FibroScan-aspartate transaminase:A superior non-invasive model for diagnosing high-risk metabolic dysfunction-associated steatohepatitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第18期30卷 2440-2453页

作者： Jing-Ya Yin tian-yuan Yang Bing-Qing Yang {2.-Xue Hou Jun-Nan li Yue li Qi Wang Center of liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing institute of infectious disease Beijing 100015China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China

BACKGROUND Non-alcoholic fatty liver disease(NAFLD)with hepatic histological NAFLD activity score≥4 and fibrosis stage F≥2.is regarded as“at risk”non-alcoholic steatohepatitis(NASH).Based on an international consensus,NAFLD and NASH were renamed as metabolic dysfunction-associated steatotic liver disease(MASLD)and metabolic dysfunction-associated steatohepatitis(MASH),respectively;hence,we introduced the term“high-risk MASH”.Diagnostic values of seven non-invasive models,including FibroScan-aspartate transaminase(FAST),fibrosis-4(FIB-4),aspartate transaminase to platelet ratio index(APRI),*** high-risk MASH have rarely been studied and compared in *** To assess the clinical value of seven non-invasive models as alternatives to liver biopsy for diagnosing high-risk *** A retrospective analysis was conducted on 309 patients diagnosed with NAFLD via liver biopsy at Beijing Ditan hospital,between January 2.12.and December *** screening for MASLD and the exclusion criteria,2.9 patients wereincluded and categorized into high-risk and non-high-risk MASH *** threshold values of each model,sensitivity,specificity,positive predictive value(PPV),and negative predictive values(NPV),were *** operating characteristic curves were constructed to evaluate their diagnostic efficacy based on the area under the curve(AUROC).RESULTS MASLD diagnostic criteria were met by 99.4%patients with *** MASLD population was analyzed in two cohorts:Overall population(2.9 patients)and the subgroup(117 patients)who underwent liver transient elastography(FibroScan).In the overall population,FIB-4 showed better diagnostic efficacy and higher PPV,with sensitivity,specificity,PPV,NPV,and AUROC of 2..9%,95.2.,73.5%,72.2.,and ***,Forns index,and aspartate transaminase to alanine transaminase ratio(ARR)showed moderate diagnostic efficacy,whereas S index and gamma-glutamyl transpeptidase to platelet ratio(GPR)were relatively *** the sub

关键词： Metabolic dysfunction-associated steatotic liver disease High-risk metabolic dysfunction-associated steatohepatitis Non-invasive models liver biopsy Diagnostic value

Analysing free way diverging risks using high-resolution trajectory data based on conflict prediction models

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transportation Safety and Environment 2024年第1期6卷 8-21页

作者： Ye li Sani Dalhatu chen {2. School of Traffic and Transportation Engineering Central South UniversityChangshaHunan 410075P.R.China department of Computer Science City University of Hong KongKowloonHong Kong 999077P.R.China.

This study aims to develop a reliable safety evaluation model for diverging vehicles and investigates the impact of the surrounding traffic environment on freeway diverging ***-resolution trajectory data from three sites in the Netherlands(Delft,Ter-Heide and Zonzeel)were employed for the risk *** regression(LR),support vector machine(SVM),random forest(RF),extreme randomize trees(ET),adaptive boosting(Adaboost),extreme gradient boosting(XGboost)and multilayer perceptron(MLP),were developed for safety *** result showed that MLP outperforms the other models for diverging risk prediction over all the indicators,conflict thresholds and *** matrix,shapely addictive explanation(SHAP),and LR algorithms were further adopted to interpret the influence of the surrounding *** indicates that an increase in traffic density,subject vehicle(SV)lateral speed,the distance of SV from ramp nose and SV length would increase the diverging *** the same time,an increase in leading vehicle speed and space headway would decrease diverging ***,spatial analysis was also conducted to explore the stability of identified traffic features regarding the impact on the diverging risk across the sites.

关键词： diverging risk,machine learning shapely addictive explanation(SHAP) linear regression(LR) extended time-to-collision(TET) time-integrated time-to-collision(TIT)

Diagnosis and treatment experience of atypical hepatic cystic echinococcosis type 1 at a tertiary center in China

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第5期30卷 462-470页

作者： Yu-Peng li Jie Zhang Zhi-De li Chao Ma Guang-Lei {2. yuan Meng Xiong chen Zhi-Gang Ma department of Hepatobiliary {2. People's Hospital of Xinjiang Uygur Autonomous RegionUrumqi 830000Xinjiang Uygur Autonomous RegionChina

BACKGROUND Some hydatid cysts of cystic echinococcosis type 1(CE1)lack well-defined cyst walls or distinctive endocysts,making them difficult to differentiate from simple hepatic *** To investigate the diagnostic methods for atypical hepatic CE1 and the clinical efficacy of laparoscopic *** The clinical data of 93 patients who had a history of visiting endemic areas of CE and were diagnosed with cystic liver lesions for the first time at the People's hospital of Xinjiang Uygur Autonomous Region(China)from January 2.18 to September 2.2. were retrospectively *** diagnoses were made based on findings from serum immunoglobulin tests for echinococcosis,routine abdominal ultrasound,high-frequency ultrasound,abdominal computed tomography(CT)scan,and *** to the treatments,these patients underwent reexaminations at the outpatient clinic until October *** evaluations included the diagnostic precision of diverse examinations,the efficacy of surgical approaches,and the incidence of CE *** All 93 patients were diagnosed with simple hepatic cysts by conventional abdominal ultrasound and abdominal CT *** them,16 patients were preoperatively diagnosed with atypical CE1,and 77 were diagnosed with simple hepatic cysts by high-frequency *** the 16 patients preoperatively diagnosed with atypical CE1 underwent laparoscopy,of whom 14 patients were intraoperatively confirmed to have CE1,which was consistent with the postoperative pathological diagnosis,one patient was diagnosed with a mesothelial cyst of the liver,and the other was diagnosed with a hepatic cyst combined with local *** the 77 patients who were preoperatively diagnosed with simple hepatic cysts,4 received aspiration sclerotherapy of hepatic cysts,and 19 received laparoscopic *** patients were intraoperatively diagnosed with simple hepatic *** the followup period,none of the 14 patients with CE1 expe

关键词： Hepatic echinococcosis Hepatic cystic echinococcosis type 1 Hepatic cyst Color Doppler ultrasound Laparoscopy

Optimal geometrical configuration and oxidation state of cobalt cations in spinel oxides to promote the performance of li-O_(2. battery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2024年第1期17卷 221-227页

作者： Yu Zhang Shuting Zhang Mengwei yuan Yufeng li Rong liu Caiyun Nan chen chen Engineering Research Center of Advanced Rare Earth Materials Department of ChemistryTsinghua UniversityBeijing 100084China Beijing Key Laboratory of Energy Conversion and Storage Materials Institution College of ChemistryBeijing Normal UniversityBeijing 100875China X-ray diffraction Lab Analytical and Testing CenterBeijing Normal UniversityBeijing 100875China

Co_(3)O_(4) is considered as one of promising cathode catalysts for lithium oxygen(li-O_(2.)batteries,which contains both tetrahedral Co^(2.)sites(Co^(2.)Td)and octahedral Co^(3+)sites(Co^(3+)Oh).It is important to reveal the effect of optimal geometric configuration and oxidation state of cobalt ion in Co_(3)O_(4) to improve the performance of li-O_(2. ***,through regulating the synthesis process,Co^(2.)and Co^(3+)sites in Co_(3)O_(4) were replaced with Zn and Al atoms to form materials with a unique Co *** li-O_(2. batteries based on ZnCo_(2.O_(4) showed longer cycle life than that of CoAl_(2.O_(4),suggesting that in Co_(3)O_(4),the Co^(3+)Oh site is a relatively better geometric configuration than Co^(2.)Td site for li-O_(2. *** calculations revealed that Co^(3+)Oh sites provide higher catalysis activity,regulating the adsorption energy of the intermediate liO_(2. and accelerating the kinetics of the reaction in batteries,which further leads to the change of the morphology of the discharge product and ultimately improves the electrochemical performance of the batteries.

关键词： lithium oxygen(li-O_(2.)batteries geometrical configuration oxidation state lithium peroxide

Pars plana vitrectomy with internal limiting membrane plugging assisted by autologous blood for optic disc pit associated with vitreomacular traction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第10期17卷 1957-1959页

作者： Bin chen Yan-Ting li {2.-Hong Wu Ya-Ping yuan Yun-Feng Lu department of Ophthalmology Suzhou EENT HospitalSuzhou 215000Jiangsu ProvinceChina department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina

Dear Editor,We report a case of optic disc pit(ODP)associated with vitreomacular traction which has been successfully treated by a combined *** have obtained the written informed consent from the patient,and this case... 详细信息

关键词： technique. traction macular