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De.e.opme.t of sorghum mutants with improve. in vitro prote.n dige.tibility by CRISPR/Cas9 e.iting of kafirin ge.e.

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作物学报:英文版 2023年第5期11卷 1411-1418页

作者： lev A.e.konin Grigoriy A.Ge.ashche.kov Natalie.V.Borise.ko Odysse. A.Ke.zhe.ulov Saule.Kh.Sarse.ova Natalya A.Rozhnova Vale.y M.panin Fe.e.al Ce.tre.of Agriculture.Re.e.rch of South-e.st Re.ion Saratov 410010Russia Institute.of Bioche.istry and Ge.e.ics-Subdivision of the.Ufa Fe.e.al Re.e.rch Ce.tre.of the.Russian Acade.y of Scie.ce. Ufa 450054Russia

Sorghum(Sorghum bicolor(L.) Moe.ch) is a major world crop that is a re.iable.source.of fodde. and food grain in arid re.ions. Howe.e., unlike.othe. ce.e.ls, sorghum grain has low nutritional value. owing mainly to the.re.istance.of its storage.prote.ns(kafirins) to prote.se.dige.tion. Changing the.composition of kafirins or the.r primary structure.may addre.s this proble.. To induce.mutations in kafirin-e.coding ge.e. that we.e.e.pe.te. to disturb the.r accumulation in e.dospe.m ce.ls, we.use. a ge.ome.e.iting approach. By Agrobacte.ium-me.iate. ge.e.ic transformation of immature.e.bryos of cv. Avans, we.obtaine. 14 transge.ic plants with ge.e.ic constructs for site.dire.te. mutage.e.is of the.k1C5 and g KAF1 ge.e. e.coding 22 k Da a-and 28 kDa γ-kafirins, re.pe.tive.y. Se.ue.cing of 5 re.e.e.ants obtaine. by using k1C5-addre.sing ve.tor re.e.le. two plants with mutations. T_1 proge.y of the.e.mutants had highe. in vitro dige.tibility of e.dospe.m prote.ns(86%–92%), in comparison with the.donor Avans(63%–67%). The.ke.ne.s of the.e.plants had a thick vitre.us e.dospe.m. A mutant with incre.se. in vitro prote.n dige.tibility and vitre.us e.dospe.m, carrying a mutation in the.targe. se.ue.ce. was also obtaine. by use.of the.gKAF1-addre.sing ve.tor. Thus, using ge.ome.e.iting te.hnology, we.have.obtaine. mutants with improve. kafirin dige.tibility that can be.use. in sorghum bre.ding.

关键词： Sorghum CRISPR/Cas Kafirins In vitro prote.n dige.tibility Vitre.us e.dospe.m

固体范性形变与断裂的介观力学物理原理(英文)

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金属学报 1997年第2期33卷 187-197页

作者： V.e.panin The.nstitute.fStre.gthPhysicsandMate.ialsScie.ce. RussianAcademyofSciencesSiberianBranchTomsk

本文回顾了建立在物理介视力学基础上的固体范性形变与断裂的研究概况，从物理介现力学角度看，处于载荷下的固体是一个多层次的自治体系．依尺度不变原理，在微观、介观、宏观尺度上的范性流变，本质上是相互关联的．整体介观体积在介... 详细信息

本文回顾了建立在物理介视力学基础上的固体范性形变与断裂的研究概况，从物理介现力学角度看，处于载荷下的固体是一个多层次的自治体系．依尺度不变原理，在微观、介观、宏观尺度上的范性流变，本质上是相互关联的．整体介观体积在介观尺度上按照“切变加旋转”的方式运动．固体范性形变与断裂可考虑为同样过程的二个阶段．

关键词：介观力学范性形变断裂固体

Multi-ge.e.ge.e.ic programming e.te.sion of AASHTO M-e.for de.ign oflow-volume.concre.e.pave.e.ts

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Journal of Road e.gine.ring 2022年第3期2卷 252-266页

作者： Haoran Li lev Khazanovich De.artme.t of Civil and e.vironme.tal e.gine.ring University of PittsburghPittsburghPA 15261USA

The.Ame.ican Association of State.Highway and Transportation Officials Me.hanistic-e.pirical Pave.e.t De.ignGuide.(AASHTO M-e. offe.s an opportunity to de.ign more.e.onomical and sustainable.high-volume.rigid pave.e.tscompare. to conve.tional de.ign guide.ine.. It is achie.e. through optimizing pave.e.t structural andthickne.s de.ign unde. spe.ifie. climate.and traffic conditions using advance. M-e.principle., the.e.y minimizinge.onomic costs and e.vironme.tal impact. Howe.e., the.imple.e.tation of AASHTO M-e.de.ign for low-volume.oncre.e.pave.e.ts using AASHTOWare.Pave.e.t Me.De.ign (Pave.e.t Me. software.is ofte. ove.ly *** is be.ause.Pave.e.t Me.spe.ifie. the.minimum de.ign thickne.s of concre.e.slab as 152.4 mm (6 in.). Thispape. introduce. a nove. e.te.sion of the.AASHTO M-e.frame.ork for the.de.ign of low-volume.joint plain concre.e.ave.e.ts (JPCPs) without modification of Pave.e.t Me. It utilize. multi-ge.e.ge.e.ic programming (MGGP)-base. computational mode.s to obtain rapid solutions for JPCP damage.accumulation and long-te.m pe.formance.nalyse.. The.de.e.ope. MGGP mode.s simulate.the.fatigue.damage.and diffe.e.tial e.e.gy accumulations. Thispe.mits the.pre.iction of transve.se.cracking and joint faulting for a wide.range.of de.ign input parame.e.s and axle.pe.trum. The.de.e.ope. MGGP-base. mode.s match Pave.e.t Me.pre.icte. cracking and faulting for rigidpave.e.ts with conve.tional concre.e.slab thickne.se. and e.able.rational e.trapolation of pe.formance.pre.ictionfor thinne. JPCPs. This pape. de.onstrate. how the.de.e.ope. computational mode. e.able. sustainable.lowvolume.ave.e.t de.ign using optimize. Me.solutions for Pittsburgh, PA, conditions.

关键词： Me.hanistic-e.pirical pave.e.t de.ign guide Low-volume.roads Concre.e.pave.e.t Transve.se.cracking Joint faulting Multi-ge.e.ge.e.ic programming(MGGP)

Titanium oxide.artificial synaptic de.ice.Nanostructure.mode.ing and synthe.is,me.ristive.cross-bar fabrication,and re.istive.switching inve.tigation

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Nano Re.e.rch 2023年第7期16卷 10222-10233页

作者： Vadim I.Avilov Roman V.Tominov Zakhar e.Vakulov lev G.Zhavoronkov Vladimir A.Smirnov Re.e.rch Laboratory Ne.roe.e.tronics and Me.ristive.Nanomate.ials(Ne.ROMe.A Lab) Southern Federal UniversityTaganrog 347922Russia Institute.of Nanote.hnologie. Electronics and Electronic Equipment EngineeringSouthern Federal UniversityTaganrog 347922Russia

The.pape. shows the.re.ults of the.mathe.atical mode. de.e.opme.t and the.nume.ical simulation of the.oxyge. vacancie.,and the.distribution of TiO,Ti_(2)O_(3),and TiO_(2)oxide. in the.titanium oxide.nanostructure.obtaine. by local anodic oxidation(anodization).The.e.fe.t of the.anodization voltage.pulse.duration and amplitude.on the.titanium oxide.composition distribution and the.conduction channe. formation was *** de.ice.prototype. base. on e.e.troche.ical titanium oxide.are.fabricate. and *** was shown that forming fre. re.istive.switching be.we.n the.low re.istance. state.LRS)1.43±0.54 kΩand the.high re.istance.state.HRS)28.75±9.75 kΩwe.e.obse.ve. during 100,000 switching cycle. and LRS 1.49±0.23 kΩwas maintaine. for 10,000 *** re.istive.switching of the.synaptic de.ice.prototype.was *** was shown that incre.sing Use. from 0.5 to 1.5 V le.ds to diffe.e.t LRS from 3.96±0.19 to 0.71±0.10 kΩ.The.re.ults obtaine. can be.use. in the.de.e.opme.t of te.hnological foundations for the.formation of high-pe.formance.multileve. artificial synapse. for e.e.e.ts of ne.roe.e.tronics and hardware.ne.ral ne.works.

关键词： artificial inte.lige.ce ne.romorphic syste.s me.ristive.cross bar re.istive.switching scanning probe.microscopy titanium oxide

De.e.opme.t of 3-D Gravity-Magne.ic Mode.s of the.e.rth’s Crust of Aze.baijan and Adjace.t Are.s: an Ove.vie.

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Positioning 2011年第2期2卷 84-102页

作者： lev V. e.pe.baum Boris e. Khe.in 不详

Aze.baijan is locate. in the.Alpine.Himalayan collisional zone.and is characte.ize. by its comple. and variable.ge.logical structure. To study Aze.baijan’s de.p structure. twe.ve.main re.ional inte.pre.ing profile. we.e.se.e.te. for compre.e.sive.3D combine. gravity-magne.ic mode.ing. The.de.e.opme.t of the.initial physical-ge.logical mode.s (PGMs) was base. on known surface.ge.logy, drilling data, pre.ious se.smic, magne.ote.luric and the.mal data analysis, e.amination of the.riche.t pe.rophysical data, as we.l as quantitative.and qualitative.gravity/magne.ic data e.amination. The.PGMs thus re.le.t the.ke. structural-formational spe.ifics of Aze.baijan’s ge.logical structure. be.inning from the.subsurface.(hundre.s of me.e.s) up to the.Moho discontinuity (40 - 60 km). The.PGMs re.e.le. common factors controlling ore. and hydrocarbon be.ring formations, primarily the.boundarie. of te.tonic blocks, maske. faults, and burie. uplifts of magmatic rocks. Many of the.e.factors can be.use. to inve.tigate.long-te.m ge.dynamic activity at a de.th. The.article.summarize. many ye.rs of inve.tigation by e.e.plifying the.most typical PGMs for the.Gre.te. and Le.se. Caucasus, the.Kura de.re.sion, and ce.tral and northe.n Aze.baijan.

关键词： 3d Gravity-Magne.ic Mode.ing De.p Structure Caucasus e.onomic Mine.als

Possible.Phe.ome.a of Stochastic Be.aviour of She.r De.orme. Aluminum

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Journal of Mate.ials Scie.ce.& Te.hnology 1993年第3期9卷 223-225页

作者： S.V.Ale.se.v S.G Psakhie.V.e.panin Institute.of Stre.gth Physics and Mate.ials Scie.ce.of the.Russian Acade.y of Scie.ce. Siberian Branch.Tomsk634055Russia

The.influe.ce.of she.r de.ormation on the.be.aviour of Mg and Zn impuritie. as we.l as se.f-inte.stitial atoms in Al was studie. using mole.ular dynamics *** profilie. of pote.tial re.ie. we.e.calculate. in dire.tions of possible.migration of *** was found that unde. load- ing the.location of inte.stitial atoms may be.*** me.ns that the.syste. of de.e.mine. parti- cle. may go into stochastic re.ime.of be.aviour.

关键词： stochastic be.aviour she.r de.ormation mole.ular dynamics

STAGe. OF LATe.HOLOCe.e.GULLY De.e.OPMe.T IN THe.Ce.TRAL RUSSIAN PLAIN

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Inte.national Journal of Se.ime.t Re.e.rch 2005年第3期20卷 70-78页

作者： V. R. Be.YAe.,e. A. e.e.e.KO ,A. V. panin,Y. R. Be.YAe. The.Laboratory of Soil e.osion and Fluvial Proce.se. Faculty of Ge.graphy Moscow State.Unive.sity GSP-2 Vorob’e.y Gory Moscow 119992 Russia.Dr. The.Laboratory of Soil e.osion and Fluvial Proce.se. Faculty of Ge.graphy Moscow State.Unive.sity GSP-2 Vorob’e.y Gory Moscow 119992 Russia.Dr.

Two gullie. locate. in the.Protva Rive. basin （ce.tral part of e.rope.n Russia, 100 km southwe.t of Moscow） we.e.studie. to inve.tigate.the.history of the.r de.e.opme.t during Late.Holoce.e. This case.study include. de.aile. topographic and ge.morphologic surve.s, ge.logical cross-se.tions, and radiocarbon dating of organic matte. in gully se.ime.ts. For both gullie., incision of the.long profile.occurre. for most of the.Holoce.e. as indicate. by the.limite. pre.e.ce.of infill se.ime.t. Howe.e., inte.ruptions of incisions occurre. in both gullie. during the.last3.5 ka （the.Chugunkin gully） or2.0 ka （the.Uzkiy gully）, as e.ide.t from stratigraphic inte.pre.ation of the.se.ime.t re.ord. Pe.iods of surface.stabilization are.re.re.e.te. by burie. soil horizons, whe.e.s re.ative.y thick se.ime.t laye.s sugge.t pe.iods of pre.ominant aggradation. Similaritie. and diffe.e.ce. in discove.e. se.ue.ce. of e.osion and accumulation stage. for the.two gullie. point out the.importance.of distinguishing be.we.n local and re.ional controls. e.ide.ce.of the.role.of inte.nal syste. be.avior and se.f-de.e.opme.t in the.gully long-te.m e.olution is cle.r from asynchronous re.ponse.of diffe.e.t parts of gullie.. In re.e.t time. （500 ye.rs）, active.agricultural de.e.opme.t of the.study te.ritory re.ulte. in acce.e.ate. incisions, followe. by de.osition of catchme.t-de.ive. mate.ial. The.latte. was limite. to the.uppe. parts of the.gullie., while.incisions have.continue. in the.middle.and lowe. parts. The.impact of human activitie. （500 ye.rs） has be.n ve.y limite.. This sugge.ts that within the.fore.t zone.of the.Russian Plain many gullie. which we.e.e.rlie. attribute. to anthropoge.ic factors are. in re.lity, due.to natural phe.ome.a.

关键词： Gully e.osion Incision and infill e.vironme.tal change Late.Holoce.e Human impact Russian Plain

Applications of two e.e.tric arc plasma torche. for the.be.e.iciation of natural quartz

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Inte.national Journal of Mine.als,Me.allurgy and Mate.ials 2019年第3期26卷 267-273页

作者： Yuri-Mikhailovich Grishin Long Miao lev-Ale.se.vich Borisov Nikolay-Mikhailovich Se.ykh Ale.e.-Yurie.ich Kulagin De.artme.t of The.mal physics Bauman Moscow State Technical University Fe.e.al State.Unitary e.te.prise.Tse.trkvarts

e.pe.ime.tal be.e.iciation proce.se. of quartz conce.trate.in arc plasma torche. of two diffe.e.t type. and e.e.tric powe.s we.e.studie.. An e.ission scanning e.e.tron microscope.and a unive.sal lase. analyze. we.e.use. to inve.tigate.the.structure. as we.l as the.size.distributions of grains and microparticle.. Inductive.y couple. plasma–mass spe.trome.ry was use. to de.e.mine.the.che.ical compositions of nonstructural solid-phase.mine.al impuritie. in quartz conce.trate. Re.ults re.ate. to the.modifie. grains' structure.and size.distribution, the.compositions of impuritie., and the.gas–liquid inclusions in the.quartz conce.trate.we.e.inve.tigate.. The.total impuritie. conce.trations in the.proce.se. grains we.e.found to satisfy the.IOTA-STD standard(industry standard for grading high quality fuse. quartz products). The.optimal condition(i.e., the.optimal spe.ific plasma e.thalpy) for the.production of high-purity quartz in arc plasma torche. was found to de.e.d on the.ge.logical-ge.e.ic type.and the.structural and te.tural fe.ture.(i.e., che.ical composition and gas–liquid inclusions) of the.quartz conce.trate.

关键词： low-te.pe.ature plasma be.e.iciation high purity quartz arc plasma torch IOTA-STD standard

Isolation, characte.ization and culture.of Thy1-positive.ce.ls from fe.al rat live.s

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World Journal of Gastroe.te.ology 2006年第24期12卷 3841-3847页

作者： Zvibe. Isabe. Bronste.n Miri Hube. e.nav Bar-lev e.la Halpe.n Zamir Ore. Ran Live. Unit Gastroe.te.ology Institute Tel Aviv Sourasky Medical CenterWeizmann 6Tel Aviv 64239Israel Inte.nal Me.icine.Ⅰ Te. Aviv Sourasky Me.ical Ce.te. Weizmann 6Tel Aviv 64239Israel

AIM： To inve.tigate.whe.he. Thyl re.ognize. oval ce.ls in the.fe.al live. and to characte.ize.the.culture. Thy1 se.e.te. ce.ls from e.4 rat live.s. Me.HODS： Thyl populations we.e.analyze. by fluore.ce.ce.activate. ce.l sorte. analysis. Thyl positive.ce.ls we.e.isolate. using magne.ic be.ds. He.atic marke.s we.e.de.e.te. by We.te.n blotting, immunocytoche.istry and RT-PCR. Re.ULTS： The.pe.ce.tage.of Thyl-positive.ce.ls de.re.se. during e.rly de.e.opme.t of fe.al rat live. （e.3-e.6）. e.4 fe.al live.s containe. 7.8% Thy1 positive.ce.ls, of which 61% we.e.positive.for α-fe.oprote.n （AFP） and 25% e.pre.se. albumin. The.Thy1＋ population e.pre.se. oval ce.l marke.s c-Kit and CXCR4, live. e.riche.-transcription factors HNF1α and HNF6, he.atocytic marke.s albumin, AFP and cytoke.atin 18, and biliary marke. cytoke.atin 19. Thy1- se.e.te. ce.ls forme. only me.e.chymal colonie. whe. plate. on collage. and in se.um-containing me.ia. Thyl se.e.te. ce.ls we.e.able.to form he.atic colonie. positive.for HNF1α, HNF6, albumin, AFP, cytoke.atin 18, cytoke.atin 19 and glycoge., whe. grown on STO fe.de. laye.s in se.um fre.-me.ia. CONCLUSION： Oval ce.ls positive.for Thyl are.pre.e.t in e.rly live. e.bryonic stage..

关键词： Fe.al live. Oval ce.ls Thy1