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High plant density increases seed Bt endotoxin content in Bt transgenic cotton

Journal of integrative Agriculture 2021年第7期20卷 1796-1806页

作者： CHEN Yuan LiU Zhen-yu HENG Li leila i.mtambel CHEN De-hua Jiangsu Key Laboratory of Crops Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou 225009P R.China Agricultural Research Cooperation Biotechnology and Biosafety Research CenterKhartoum 999129Sudan

Plant density is the cultivation practice usually employed to manipulate boll distribution, boll setting and yield in cotton production. in order to determine the effect of plant density on the insecticidal protein content of Bacillus thuringiensis(Bt) cotton plants, a study was conducted in Yangzhou University of China in 2015 and 2016. Five plant densities(PD1–PD5, representing 15 000, 30 000, 45 000, 60 000, and 75 000 plants ha^(–1)) were imposed on two Bt cotton cultivars, Sikang 1(the conventional cultivar, SK-1) and Sikang 3(the hybrid cultivar, SK-3). The boll number per plant, boll weight and boll volume all decreased as plant density increased. As plant density increased from 15 000 to 75 000 plants ha–1, seed Bt protein content increased, with increases of 66.5% in SK-1 and 53.4% in SK-3 at 40 days after flowering(DAF) in 2015, and 36.8% in SK-1 and 38.6% in SK-3 in 2016. Nitrogen(N) metabolism was investigated to uncover the potential mechanism. The analysis of N metabolism showed enhanced soluble protein content, glutamic-pyruvic transaminase(GPT) and glutamate oxaloacetate transaminase(GOT) activities, but reduced free amino acid content, and protease and peptidase activities with increasing plant density. At 20 DAF, the seed Bt toxin amount was positively correlated with soluble protein level, with correlation coefficients of 0.825** in SK-1 and 0.926** in SK-3 in 2015, and 0.955** in SK-1 and 0.965** in SK-3 in 2016. in contrast, the seed Bt protein level was negatively correlated with free amino acid content, with correlation coefficients of –0.983** in SK-1 and –0.974** in SK-3 in 2015, and –0.996** in SK-1 and –0.986** in SK-3 in 2016. To further confirm the relationship of Bt protein content and N metabolism, the Bt protein content was found to be positively correlated with the activities of GPT and GOT, but negatively correlated with the activities of protease and peptidase. in conclusion, our present study indicated that high plant density elev

关键词： Bt cotton plant density Bt insecticidal protein nitrogen metabolism

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improving Channel Estimation in a NOMA Modulation Environment Based on Ensemble Learning

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Computer Modeling in Engineering & Sciences 2024年第8期140卷 1315-1337页

作者： Lassaad K.Smirani leila Jamel Latifah Almuqren Elearning Deanship and Distance Education Umm Al-Qura UniversityP.O.Box 715MekkahSaudi Arabia Department of information Systems College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia

This study presents a layered generalization ensemble model for next generation radio mobiles,focusing on supervised channel estimation *** estimation typically involves the insertion of pilot symbols with a well-balanced rhythm and suitable *** model,called Stacked Generalization for Channel Estimation(SGCE),aims to enhance channel estimation performance by eliminating pilot insertion and improving *** SGCE model incorporates six machine learning methods:random forest(RF),gradient boosting machine(GB),light gradient boosting machine(LGBM),support vector regression(SVR),extremely randomized tree(ERT),and extreme gradient boosting(XGB).By generating meta-data from five models(RF,GB,LGBM,SVR,and ERT),we ensure accurate channel coefficient predictions using the XGB *** validate themodeling performance,we employ the leave-one-out cross-validation(LOOCV)approach,where each observation serves as the validation set while the remaining observations act as the training *** performances’results demonstrate higher mean andmedian accuracy compared to the *** achieves an average accuracy of 98.4%,precision of 98.1%,and the highest F1-score of 98.5%,accurately predicting channel ***,our proposedmethod outperforms prior traditional and intelligent techniques in terms of throughput and bit error ***’s superior performance highlights its efficacy in optimizing channel *** can effectively predict channel coefficients and contribute to enhancing the overall efficiency of radio mobile *** extensive experimentation and evaluation,we demonstrate that SGCE improved performance in channel estimation,surpassing previous ***,SGCE’s capabilities have significant implications for optimizing channel estimation in modern communication systems.

关键词： Stacked generalization ensemble learning Non-Orthogonal Multiple Access(NOMA) channel estimation 5G

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inhibition of protein degradation increases the Bt protein concentration in Bt cotton

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Journal of integrative Agriculture 2024年第6期23卷 1897-1909页

作者： Yuting Liu Hanjia Li Yuan Chen Tambel leila.i.M Zhenyu Liu Shujuan Wu Siqi Sun Xiang Zhang Dehua Chen Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Agricultural Research Cooperation Biotechnology and Biosafety Research CenterKhartoum 13314Sudan

Bacillus thuringiensis(Bt)cotton production is challenged by two main problems,i.e.,the low concentration of Bt protein at the boll setting stage and the lowest insect resistance in bolls among all the cotton plant’s ***,increasing the Bt protein concentration at the boll stage,especially in bolls,has become the main goal for increasing insect resistance in *** this study,two protein degradation inhibitors(ethylene diamine tetra acetic acid(EDTA)and leupeptin)were sprayed on the bolls,subtending leaves,and whole cotton plants at the peak flowering stage of two Bt cultivars(medium maturation Sikang 1(SK1))and early maturation Zhongmian 425(ZM425)in 2019 and *** Bt protein content and protein degradation metabolism were *** results showed that the Bt protein concentrations were enhanced by 21.3 to 38.8%and 25.0 to 38.6%in the treated bolls of SK1 and ZM425 respectively,while they were decreased in the subtending leaves of these treated *** the treated leaves,the Bt protein concentrations increased by 7.6 to 23.5%and 11.2 to 14.9%in SK1 and ZM425,*** combined application of EDTA and leupeptin to the whole cotton plant increased the Bt protein concentrations in both bolls and subtending *** Bt protein concentrations in bolls were higher,increasing by 22.5 to 31.0%and 19.6 to 32.5%for SK1 and ZM425,*** organs treated with EDTA or/and leupeptin showed reduced free amino acid contents,protease and peptidase activities and significant enhancements in soluble protein *** results indicated that inhibiting protein degradation could improve the protein content,thus increasing the Bt protein concentrations in the bolls or/and leaves of cotton ***,the increase in the Bt protein concentration without yield reduction suggested that these two protein degradation inhibitors may be applicable for improving insect resistance in cotton production.

关键词： Bt cotton Bt protein inhibition of protein degradation protein degradation metabolism

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Tracing suspended and bed sediments during high and low water periods using geochemical characteristics-Case study:Vazrood watershed,northern iran

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Journal of Mountain Science 2024年第2期21卷 470-483页

作者： KARiMi Nabiyeh GHOLAMi leila KAViAN Ataollah KHALEDi DARViSHAN Abdulvahed Department of Watershed Management Faculty of Natural ResourcesSari Agricultural Sciences and Natural Resources UniversitySari ***Iran Department of Watershed Management Engineering Faculty of Natural ResourcesTarbiat Modares UniversityNoor ***Iran

Complete and comprehensive information about sediment dynamic and identification of hotspots of sediment production and transport are necessary for understanding the erosion processes and increasing the efficiency of soil and water conservation *** studies used the sediment fingerprint techniques to investigate the contribution of different sources in suspended and bed sediment yield of the ***,the contribution of various land use/land covers in suspended and bed sediment yield for the great Caspian Sea basin is in an aura of ambiguity and the present study was conducted to gather information about an important part of this area in northern iran,where rangelands are located upstream of Hyrcanian forests and dense agricultural lands are located *** surface soil of different land use/land covers including forest,rangeland,agriculture and streambank lands were sampled in 30 *** and bed sediments were sampled in the watershed outlet in two high and low water *** characteristics of soil and sediment samples containing 59 elements were measured using iCP-OES GBC *** reliable and suitable tracers from 59 elements were then selected using Range test,Kruskal-Wallis and Discriminant Function Analysis,respectively,in FingerPro package of R *** results showed that for suspended sediment,streambank and rangeland had the highest contributions of 86.2%and 47.5%,respectively,in two high and low water *** bed sediment,in two high and low water periods,rangeland and streambank had the highest contributions of 73.8%and 84.4%,*** use change and especially human activities such as agriculture,road construction and development of residential areas along the main river riparian zone has led to a significant increase in suspended and bed sediments.

关键词： Caspian Sea basin FingerPro Geochemistry Sediment transport Tracer combination

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Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo

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Asian Pacific Journal of Tropical Biomedicine 2024年第4期14卷 154-161页

作者： Fairouz Sioud Aida Lahmer Mouna Selmi Fadwa Chaabane leila Chekir-Ghedira Research Unit Bioactive Natural Products and Biotechnology UR17ES49 Faculty of Dental Medicine of MonastirUniversity of MonastirAvicenne Street5000 MonastirTunisia Laboratory of Pharmacognosy Faculty of PharmacyUniversity of MonastirAvicenne Street5000 MonastirTunisia

Objective:To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in ***:The effects of the methanolic extract of Ephedra alata on the viability,migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,Transwell assay,and annexin V-FiTC staining assay,*** examination was also carried ***,a murine breast cancer model was established to evaluate the inhibitory effect of the *** parameters including hepatic and non-hepatic enzymes,malondialdehyde,and glutathione were ***:The methanolic extract of Ephedra alata showed a strong anti-proliferative and anti-migratory activity against 4T1 cells in a dose-dependent *** also induced apoptosis in 4T1 *** an in vivo mouse model,the extract markedly inhibited tumor growth,reduced malondialdehyde,and hepatic and non-hepatic enzymes as well as increased glutathione ***:The methanolic extract of Ephedra alata inhibits breast cancer in vitro and in vivo,which may be a promising anticancer agent.

关键词： Ephedra Breast cancer Apoptosis Antioxidant Migration

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Evaluation of Mechanical Properties and Surface Quality of Wood from Bosnia and Herzegovina Exposed to Outdoor Conditions

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Journal of Renewable Materials 2024年第8期12卷 1417-1431页

作者： Redžo Hasanagić UmejrŠljivo leila Fathi Pallavi Gautam Mohsen Bahmani Miha Humar Department of Wood Science and Technology Faculty of Technical EngineeringUniversity of BihaćBihać77000Bosnia and Herzegovina Department of Wood and Furniture industry Engineering Faculty of Resources and Earth ScienceShahrekord UniversityShahrekord64165478Iran Department of Botany Institute of ScienceBanaras Hindu UniversityVaranasiUttar Pradesh221005India Biotechnical Faculty Department of Wood ScienceUniversity of LjubljanaLjubljana1501Slovenia

This study investigated the mechanical properties of beech(Fagus sylvatica L.)and fir(Abies alba)wood from Bosnia and Herzegovina under outdoor *** were exposed for 3-month exposure to assess bending strength,color changes,and surface *** showed outdoor exposure negatively affected mechanical properties,particularly in samples with extended finger joints,causing significant surface cracks in uncoated *** wood exhibited notable color changes under exposure,with approximately 50%darkening without coating compared to 25%under covered *** samples displayed minimal color changes,affirming the efficacy of surface *** wood exhibited a roughness of 8.264μm,while beechwood average roughness increased from 6.767 to 13.916μm after exposure,with micro-pore development affecting water *** analysis identified prevalent fungal colonies,including Penicillium,Aureobasidium,Sclerophoma,and Chaetomium,underscoring their role in organic matter *** study highlights the importance of wood exposure and treatment selection for various applications.

关键词： Mechanical properties outdoor conditions cracks wood species

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Deciphering resistancemechanisms and novel strategies to overcome drug resistance in ovarian cancer:a comprehensive review

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Oncology Research 2024年第5期32卷 831-847页

作者： EFFAT ALEMZADEH leila ALLAHQOLi AFROOZ MAZiDiMORADi ESMAT ALEMZADEH FAHiMEH GHASEMi HAMiD SALEHiNiYA iBRAHiM ALKATOUT infectious Diseases Research Center Birjand University of Medical SciencesBirjandIran Department of Midwifery Ministry of Health and Medical EducationTehranIran Student Research Committee Shiraz University of Medical SciencesShirazIran Department of Biotechnology Faculty of MedicineBirjand University of Medical SciencesBirjandIran Cellular and Molecular Research Center Birjand University of Medical SciencesBirjandIran Social Determinants of Health Research Center Birjand University of Medical SciencesBirjandIran Kiel School of Gynaecological Endoscopy Campus KielUniversity Hospitals Schleswig-HolsteinKielGermany

Ovarian cancer is among the most lethal gynecological cancers,primarily due to the lack of specific symptoms leading to an advanced-stage diagnosis and resistance to *** resistance(DR)poses the most significant challenge in treating patients with existing *** Food and Drug Administration(FDA)has recently approved three new therapeutic drugs,including two poly(ADP-ribose)polymerase(PARP)inhibitors(olaparib and niraparib)and one vascular endothelial growth factor(VEGF)inhibitor(bevacizumab)for maintenance ***,resistance to these new drugs has ***,understanding the mechanisms of DR and exploring new approaches to overcome them is crucial for effective *** this review,we summarize the major molecular mechanisms of DR and discuss novel strategies to combat DR.

关键词： Chemotherapy Drug resistance mechanisms Ovarian cancer PARP inhibitors VEGF inhibitor

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Changes in sexual activity in young women during pregnancy

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Asian pacific Journal of Reproduction 2024年第5期13卷 213-218页

作者： Ghazal Mansouri Mina Zakeri Fatemeh Karami Robati leila Allahqoli Azam Dehghani Department of Obstetrics and Gynecology School of MedicineAfzalipour HospitalKerman University of Medical SciencesKermanIran Clinical Research Development Unit Afzalipour HospitalKerman University of Medical SciencesKermanIran School of Public Health Iran University of Medical Sciences(IUMS)TehranIran Gastroenterology and Hepatology Research Center Institute of Basic and Clinical Physiology SciencesKerman University of Medical SciencesKermanIran

Objective:To conduct changes in sexual activity during pregnancy and its related factors in pregnant ***:The present descriptive cross-sectional study was conducted in 2017 on pregnant women who referred to the women's clinic of Afzalipour Hospital in the southeast of *** were included in the study through convenient *** data collection tool was a researcher-made questionnaire consisting of two parts of personal social information of the couple and questions to measure the level of sexual activity and the attitude of the subjects during pregnancy compared to before ***:201 Pregnant women were *** average age of pregnant women was(27.3±6.1)years and their average gestational age was(24.7±11.8)*** than 62%of women had decreased sexual *** was a significant relationship between the amount of changes in sexual activity and delivery time(P=0.013),abortion history(P=0.001)and premature birth history(P=0.002).Most pregnant women believed that sex during pregnancy caused damage to the fetus(67.7%).A decrease in the intensity of sexual desire was reported in 63%of pregnant *** than 60%of the subjects did not consult with doctors and midwives with regards to sexual issues(63.5%).The most common reason for not consulting was not feeling the need(32%).Most women experienced back pain during(42.8%)and after(39.8%)***:Changes in sex life during pregnancy are often caused by the lack of sexual knowledge and the increase in misconceptions among couples,which can affect the quality of *** role of education is ***,it is suggested that by including sexual counseling along with pregnancy care,wrong beliefs and information among women will be corrected.

关键词： Sexual behavior Women Pregnancy Midwifery Sex counseling Fetus

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The potent osteo-inductive capacity of bioinspired brown seaweed-derived carbohydrate nanofibrous three-dimensional scaffolds

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Marine Life Science & Technology 2024年第3期6卷 515-534页

作者： Zahra Dadashi Ouranj Saadi Hosseini Atefeh Alipour Shahin Homaeigohar Shahram Azari leila Ghazizadeh Mohammadali Shokrgozar Sabu Thomas Saeed irian Hosein Shahsavarani Department of Cell and Molecular Biology Faculty of Biological SciencesKharazmi UniversityTehran 15719-14911Iran Laboratory of Regenerative Medicine and Biomedical innovations National Cell Bank of IranPasteur Institute of IranTehran 13169-43551Iran Department of Nanobiotechnology Pasteur Institute of IranTehran 13169-43551Iran School of Science and Engineering University of DundeeDundee DD14HNUK School of Chemical Sciences Mahatma Gandhi UniversityKottayamKeralaIndia Department of Cell and Molecular Biology Faculty of Life Sciences and BiotechnologyShahid Beheshti UniversityTehran 19839-69411Iran iranian Biological Resource Center Academic Center for EducationCulture and Research(ACECR)Tehran ***Iran

This study aimed to investigate the osteo-inductive capacity of a fucoidan polysaccharide network derived from brown algae on human adipose-derived stem cells(HA-MSCs)for bone *** physiochemical properties of the scaffold including surface morphology,surface chemistry,hydrophilicity,mechanical stiffness,and porosity were thoroughly *** in vitro and in vivo measurements implied a superior cell viability,proliferation,adhesion,and osteo-inductive performance of obtained scaffolds compared to using specific osteogenic induction medium with increased irregular growth of calcium crystallites,which mimic the structure of natural *** scaffold was highly biocompatible and suitable for cell *** examinations,such as quantification of mineralization,alkaline phosphatase,gene expression,and immunocytochemical staining of pre-osteocyte and bone markers confirmed that HAD-MSCs differentiate into osteoblasts,even without an osteogenic induction *** study provides evidence for the positive relationship and synergistic effects between the physical properties of the decellularized seaweed scaffold and the chemical composition of fucoidan in promoting the osteogenic differentiation of ***,the natural matrices derived from brown seaweed offers a sustainable,cost-effective,non-toxic bioinspired scaffold and holds promise for future clinical applications in orthopedics.

关键词： Bone tissue engineering Seaweed-derived scaffold Fucoidans HA-MSCs differentiation Osteoblasts

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Path-Based Clustering Algorithm with High Scalability Using the Combined Behavior of Evolutionary Algorithms

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Computer Systems Science & Engineering 2024年第3期48卷 705-721页

作者： leila Safari-Monjeghtapeh Mansour Esmaeilpour Department of Computer Engineering Sanandaj BranchIslamic Azad UniversitySanandaj65166Iran Department of Computer Engineering Hamedan BranchIslamic Azad UniversityHamedan69168Iran

Path-based clustering algorithms typically generate clusters by optimizing a benchmark *** optimiza-tion methods in clustering algorithms often offer solutions close to the general optimal *** study achieves the global optimum value for the criterion function in a shorter time using the minimax distance,Maximum Spanning Tree“MST”,and meta-heuristic algorithms,including Genetic Algorithm“GA”and Particle Swarm Optimization“PSO”.The Fast Path-based Clustering“FPC”algorithm proposed in this paper can find cluster centers correctly in most datasets and quickly perform clustering *** FPC does this operation using MST,the minimax distance,and a new hybrid meta-heuristic algorithm in a few rounds of algorithm *** algorithm can achieve the global optimal value,and the main clustering process of the algorithm has a computational complexity of O�k2×n�.However,due to the complexity of the minimum distance algorithm,the total computational complexity is O�n2�.Experimental results of FPC on synthetic datasets with arbitrary shapes demonstrate that the algorithm is resistant to noise and outliers and can correctly identify clusters of varying sizes and *** addition,the FPC requires the number of clusters as the only parameter to perform the clustering process.A comparative analysis of FPC and other clustering algorithms in this domain indicates that FPC exhibits superior speed,stability,and performance.

关键词： Clustering global optimization the minimax matrix MST path-based clustering FPC

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