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In-vitro assessment for the control of Fusarium species using a lactic acid bacterium isolated from yellow pitahaya(Selenicereus megalanthus(K. Schum. Ex Vaupel Moran))

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journal of Integrative Agriculture 2021年第1期20卷 159-167页

作者： leidy j.valencia-hernandez Karina LÓOPEZ-LÓPEZ Eyder D.GÓMEZ-LÓPEZ Liliana SERNACOCK Cristobal N.AGUILAR Universidad Nacional de Colombia Palmira CampusValle 763533Colombia Food Research Department School of ChemistryAutonomous University of CoahuilaSaltillo CampusCoahuila 25280Mexico

The fungistatic activity of a lactic acid bacterium, which had been isolated from yellow pitahaya cultures, against fungi associated with basal rot(Fusarium oxysporum and Fusarium fujikuroi) was measured in the present study. Its activity was assessed in three fractions: fermented(S1), metabolic products(S2), and biomass(S3), using two fermentation substrates: Man Rogosa Sharpe agar(MRS) and potato dextrose agar(PDA). The bacterium was molecularly identified as Lactobacillus plantarum. S3 reduced F. fujikuroi growth by 100% over 48 h of fermentation, which occurred during the stationary phase of bacterial growth. The three fractions' fungistatic activity against F. fujikuroi depended on the substrate employed. The fermentation kinetic parameters for L. plantarum indicated that its specific growth rate was 0.46 h^–1, with 93.63% substrate consumption, 0.045 kg kg^–1 cell yield, and 0.54 kg kg^–1 product yield. The kinetic parameters calculated will allow for bacteria production scaling. These in-vitro trials reveal L. plantarum's possible application as a biocontrol agent for diseases associated with Fusarium. However, further ex-vivo and in-vivo researches are required to demonstrate its behavior in crops.

关键词： lactic acid bacteria Fusarium fungistatic activity biomass yellow pitahaya

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Use of fly and bottom ashes from a thermoelectrical plant in the synthesis of geopolymers:Evaluation of reaction efficiency

Energy Geoscience

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Energy Geoscience 2021年第2期2卷 167-173页

作者： leidy j.jaramillo Nieves Fabio Elyseu Silviany Goulart Mariana de Souza Pereira Erick Zabot Valvassori Adriano Michael Bernardin Ceramic Materials Group Universidade do Extremo Sul CatarinenseAv.Universitaria 110588806-000CriciumaSanta CatarinaBrazil

The use of by-products as raw materials in the manufacturing of industrial products has risen in the last years because of environmental considerations.One example is the use of coal ashes from thermalelectrical plants in the production of geopolymer–a green cement made by mixing aluminosilicate with alkaline activator.In this study,fly and bottom ashes from a thermal-electrical unit were used as sources of aluminosilicate in the synthesis of geopolymers.A mixture of sodium hydroxide(10 mol/L)and sodium silicate(SiO_(2)/Na_(2)O ratio of 2.2)was used as the alkaline activator.The type(fly or bottom ash)and content of the ash were the variables in the synthesis.The ashes were characterized by X-ray fluorescence(XRF),X-ray diffraction(DRX),particle size distribution(PSD),specific surface area(BET),and thermal analysis(DTA/TGA).The ash-based geopolymer samples were measured to obtain their compressive strength after curing.The evolution of the geopolymerization process was also assessed based on final alkali concentration measurements.The results show that it is possible to obtain geopolymers using coal ashes as raw materials with high solid content.The compressive strength for the bottom ash geopolymer after 90 days of curing is 35 MPa.The low concentration of unreacted alkalis after curing(1.5×10^(-3)e 3.5×10^(-3)M)corresponds to high efficiency of the geopolymerization reaction.

关键词： Coal ash Geopolymer Industrial by-product Geopolymerization reaction

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Acid-Functionalized Nanoparticles for Pretreatment of Wheat Straw

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journal of Biomaterials and Nanobiotechnology 2012年第3期3卷 342-352页

作者： leidy Pena Myles Ikenberry Keith L.Hohn Donghai Wang Department of Biological and Agricultural Engineering Kansas State UniversityManhattanUSA Department of Chemical Engi-neering Kansas State UniversityManhattanUSA

Perfluoroalkylsufonic (PFS) and alkylsufonic (AS) acid-functionalized magnetic nanoparticles were synthesized and characterized, then evaluated for their ability to hydrolyze hemicelluloses. The magnetic core was made of cobalt spinel ferrite and was coated with silica to protect it from oxidation. The silanol groups allowed surface chemical modification of the nanoparticles with the PFS and AS acid functionalities. Thermogravimetric analysis gave a total organic load of 12.6% and 32.5% (w/w) for AS and PFS nanoparticles, respectively. The surface sulfur content was calculated from XPS analysis as 1.37% and 1.93% for PFS and AS nanoparticles, respectively. Wheat straw samples were treated with the acid-functionalized nanoparticles at two different conditions: 80℃ for 24 h and 160℃ for 2 h. These experiments aimed to hydrolyze wheat straw hemicelluloses to soluble oligosaccharides. PFS nanoparticles solubilized significantly higher amounts of hemicelluloses (24.0% ± 1.1%) than their alkyl-sulfonic counterparts (9.1% ± 1.7%) at 80℃, whereas the hydrothermolysis control solubilized 7.7% ± 0.8% of the original hemicelluloses in the sample. At 160℃, PFS and AS nanoparticles gave significantly higher amounts of oligosaccharides (46.3% ± 0.4% and 45 ± 1.2%, respectively) than the control (35.0% ± 1.8%). The hemicelluloses conversion at 160?C reached 66.3% ± 0.9% using PFS nanoparticles and 61.0% ± 1.2% using AS nanoparticles compared with the control experiment, which solubilized 50.9% ± 1.7% of hemicelluloses in the biomass.

关键词： Acid-Functionalized Magnetic Nanoparticles Biomass Hemicelluloses Hydrolysis

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca leidy Yasmín Vargas-Mendoza André Aptroot Laura juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres josé Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez jesús E.Hernández Marin María de los Ángeles Herrera-Campos María E.Holgado-Rojas Harald jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins joel A.Mercado-Díaz jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-Corredor jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi james D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de Caldas(FAUC) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 Chavis Avenue LexingtonVA 24450USA Charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany jardı´n Bota´nico de Bogota´jose´Celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de Ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 years.Based on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been sequenced.Species of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is provided.The new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking spec.nov.,C.applanata Moncada,Soto-Medina&Lücking spec.nov.,C.arachnodavidea Moncada,Dal Forno&Lücking spec.nov.,C.arborescens Dal Forno,Chaves&Lücking spec.nov.,C.arcabucana Moncada,C.Rodrı´guez&Lücking spec.nov.,C.aturucoa Lücking,Moncada&C.Vargas spec.nov.,C.auriculeslia Moncada,Ya´nez-Ayabaca&Lücking spec.nov.,C.barbifera Moncada,Patin˜o&Lücking spec.nov.,C.boleslia Lücking,E.Morales&Dal Forno spec.nov.,C.caliginosa Holgado,Rivas Plata&Perlmutter spec.nov.,C.campestris Dal Forno,Eliasaro&Spielmann spec.nov.,C.canari Nugra,Dal Forno&Lücking spec.nov.,C.caraana Lücking,Martins&Lucheta spec.nov.,C.casasolana Moncada,R.-E.Pe´rez&Lücking spec.nov.,C.caucensis Moncada,M.Gut.&Lücking spec.nov.,C.celestinoa Moncada,CabreraAmaya&Lücking spec.nov.,C.comaltepeca Moncada,R.-E.Pe´rez&Herrera-Camp.***.nov.,C.corani Lücking,E.Morales&Dal Forno spec.nov.,C.corelleslia Moncada,A.Sua´rez-Corredor&Lücking spec.nov.,C.crispoleslia Moncada,j.Molina&Lücking spec.nov.,C.cuzcoensis Holgado,Rivas Plata&Perlmutter spec.nov

关键词： Bolivia Citizen science Chile Mexico Puerto Rico Sri Lanka

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