检索结果-南通市图书馆

Role of oncogenic long noncoding RNA KCNQ1OT1 in colon cancer

在线全文

学校读者我要写书评

暂无评论

Oncology Research 2024年第3期32卷 585-596页

作者： GANG LIU lei shi BIN WANG ZEHUI WU HAIYUAN ZHAO TIANYU ZHAO LIANGHUI shi Department of Gastrointestinal Surgery First ffiliated Hospital of Wannan Medical CollegeWuhu241001China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Stomatological Hospital of Chongqing Medical UniversityChongqing401147China

The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remains unclear.Retrovirnl vector pSEB61 was retroftted in established HCT116 siKCN and SW480-siKCN cells to silence KCNQ1 OT1.Cellular proliferation was measured using CCK8 assay,and flow cytometry(FCM)detected cell cydle changes.RNA sequencing(RNA Seq)analysis showed differentially expressed genes(DEGs).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.RT-qPCR,immunofluorescence,and western blotting were carried out to validate downstream gene expressions.The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts.Our data revealed that the silencing of KONQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase.RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cyde.RT qPCR,immunofluorescence,and western blotting confirmed downstream CCNE2 and PCNA gene expressions.HCT116 siKCN cells signifcantly suppressed tumorigenesis in BALB/c nude mice.Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer.

关键词： lncRNA KCNQ1OT1 Colon cancer HCT116 cells Tumorigenesis

Empowering smart city situational awareness via big mobile data

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Information Technology & Electronic Engineering 2024年第2期25卷 286-307页

作者： Zhiguang SHAN lei shi Bo LI Yanqiang ZHANG Xiatian ZHANG Wei CHEN State Information Center Beijing100045China School of Computer Science and Engineering Beihang UniversityBeijing100191China Zhongguancun Laboratory Beijing100094China Beijing Tendcloud Tianxia Technology Co. Ltd.Beijing100027China The State Key Lab of CAD&CG Zhejiang UniversityHangzhou310027China

Smart city situational awareness has recently emerged as a hot topic in research societies,industries,and governments because of its potential to integrate cutting-edge information technology and solve urgent challenges that modern cities face.For example,in the latest five-year plan,the Chinese government has highlighted the demand to empower smart city management with new technologies such as big data and Internet of Things,for which situational awareness is normally the crucial first step.While traditional static surveillance data on cities have been available for decades,this review reports a type of relatively new yet highly important urban data source,i.e.,the big mobile data collected by devices with various levels of mobility representing the movement and distribution of public and private agents in the city.We especially focus on smart city situational awareness enabled by synthesizing the localization of hundreds of thousands of mobile software Apps using the Global Positioning System(GPS).This technique enjoys advantages such as a large penetration rate(∼50%urban population covered),uniform spatiotemporal coverage,and high localization precision.We first discuss the pragmatic requirements for smart city situational awareness and the challenges faced.Then we introduce two suites of empowering technologies that help fulfill the requirements of(1)cybersecurity insurance for smart cities and(2)spatiotemporal modeling and visualization for situational awareness,both via big mobile data.The main contributions of this review lie in the description of a comprehensive technological framework for smart city situational awareness and the demonstration of its feasibility via real-world applications.

关键词： Smart city Mobile data Situational awareness

The influence of yttrium and manganese additions on the degradation and biocompatibility of magnesium-zinc-based alloys:In vitro and in vivo studies

在线全文

学校读者我要写书评

暂无评论

Journal of Magnesium and Alloys 2024年第2期12卷 608-624页

作者： lei shi Yang Yan Chun-sheng Shao Kun Yu Bo Zhang Liang-jian Chen Third Xiangya Hospital Central South UniversityChangsha 410013China State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China School of Materials Science and Engineering Central South UniversityChangsha 410083China Department of Mechanical Engineering City University of Hong KongHong KongChina

The repair and regeneration of bone defects are highly challenging orthopedic problems.Recently,Mg-based implants have gained popularity due to their unique biodegradation and elastic modulus similar to that of human bone.The aim of our study is to develop a magnesium alloy with a controllable degradation that can closely match bone tissue to help injuries heal in vivo and avoid cytotoxicity caused by a sudden increase in ion concentration.In this study,we prepared and modified Mg-3Zn,Mg-3Zn-1Y,and Mg-2Zn-1Mn by hot extrusion,and used Mg-2.5Y-2.5Nd was as a control.We then investigated the effect of additions of Y and Mn on alloys'properties.Our results show that Mn and Y can improve not only compression strength but also corrosion resistance.The alloy Mg-2Zn-1Mn demonstrated good cytocompatibility in vitro,and for this reason we selected it for implantation in vivo.The degraded Mg-2Zn-1Mn implanted a bone defect area did not cause obvious rejection and inflammatory reaction,and the degradation products left no signs of damage to the heart,liver,kidney,or brain.Furthermore,we find that Mg-2Zn-1Mn can promote an osteoinductive response in vivo and the formation of bone regeneration.

关键词： Magnesium alloy Biodegradation Biocompatibility Bone regeneration Bone defect repair

Polycytosine RNA-binding protein 1 regulates osteoblast function via a ferroptosis pathway in type 2 diabetic osteoporosis

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第5期15卷 977-987页

作者： Hong-Dong Ma lei shi Hai-Tian Li Xin-Dong Wang Mao-Wei Yang Department of Orthopedics The Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Orthopedics The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND Recently,type 2 diabetic osteoporosis(T2DOP)has become a research hotspot for the complications of diabetes,but the specific mechanism of its occurrence and development remains unknown.Ferroptosis caused by iron overload is con-sidered an important cause of T2DOP.Polycytosine RNA-binding protein 1(PCBP1),an iron ion chaperone,is considered a protector of ferroptosis.AIM To investigate the existence of ferroptosis and specific role of PCBP1 in the development of type 2 diabetes.METHODS A cell counting kit-8 assay was used to detect changes in osteoblast viability under high glucose(HG)and/or ferroptosis inhibitors at different concentrations and times.Transmission electron microscopy was used to examine the morpho-logical changes in the mitochondria of osteoblasts under HG,and western blotting was used to detect the expression levels of PCBP1,ferritin,and the ferroptosis-related protein glutathione peroxidase 4(GPX4).A lentivirus silenced and overex-pressed PCBP1.Western blotting was used to detect the expression levels of the osteoblast functional proteins osteoprotegerin(OPG)and osteocalcin(OCN),whereas flow cytometry was used to detect changes in reactive oxygen species(ROS)levels in each group.RESULTS Under HG,the viability of osteoblasts was considerably decreased,the number of mitochondria undergoing atrophy was considerably increased,PCBP1 and ferritin expression levels were increased,and GPX4 expression was decreased.Western blotting results demonstrated that infection with lentivirus overexpressing PCBP1,increased the expression levels of ferritin,GPX4,OPG,and OCN,compared with the HG group.Flow cytometry results showed a reduction in ROS,and an opposite result was obtained after silencing PCBP1.CONCLUSION PCBP1 may protect osteoblasts and reduce the harm caused by ferroptosis by promoting ferritin expression under a HG environment.Moreover,PCBP1 may be a potential therapeutic target for T2DOP.

关键词： Polycytosine RNA-binding protein 1 Ferroptosis Reactive oxygen species Ferritin Osteoblast Type 2 diabetic osteoporosis

Recombinant adeno-associated virus 8 vector in gene therapy:Opportunities and challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第1期11卷 283-293页

作者： Liyuan Zhao Zixuan Yang Minhui Zheng lei shi Mengyun Gu Gang Liu Feng Miao Yan Chang Fanghua Huang Naping Tang Anhui University of Traditional Chinese Medicine HefeiAnhui 230000China Yangtze Delta Drug Advanced Research Institute Yangtze Delta Pharmaceutical CollegeNantongJiangsu226133China Shanghai Innostar Bio-Technology Co. LtdChina State Institute of Pharmaceutical IndustryShanghai 201203China Innostar Bio-tech Nantong Co. Ltd.NantongJiangsu 226133China Center for Drug Evaluation National Medical Products AdministrationBeijing 100022China

In recent years,significant breakthroughs have been made in the field of gene ther-apy.Adeno-associated virus(AAV)is one of the most promising gene therapy vectors and a powerful tool for delivering the gene of interest.Among the AAV vectors,AAV serotype 8(AAv8)has attracted much attention for its efficient and stable gene transfection into specific tissues.Currently,recombinant AAv8 has been widely used in gene therapy research on a va-riety of diseases,including genetic diseases,cancers,autoimmune diseases,and viral diseases.

关键词： AAV8 Adeno-associated virus Gene therapy Primates

Rosemary extract improves egg quality by altering gut barrier function,intestinal microbiota and oviductal gene expressions in late-phase laying hens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第1期15卷 295-312页

作者： Lianhua Zhang Junwei Ge Fei Gao Min Yang Hui Li Fei Xia Hongtong Bai Xiangshu Piao Zhiying Sun lei shi Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China College of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background Rosemary extract(RE)has been reported to exert antioxidant property.However,the application of RE in late-phase laying hens on egg quality,intestinal barrier and microbiota,and oviductal function has not been systematically studied.This study was investigated to detect the potential effects of RE on performance,egg quality,serum parameters,intestinal heath,cecal microbiota and metabolism,and oviductal gene expressions in late-phase laying hens.A total of 21065-week-old“Jing Tint 6”laying hens were randomly allocated into five treatments with six replicates and seven birds per replicate and fed basal diet(CON)or basal diet supplemented with chlortetracycline at 50 mg/kg(CTC)or RE at 50 mg/kg(RE50),100 mg/kg(RE100),and 200 mg/kg(RE200).Results Our results showed that RE200 improved(P<0.05)Haugh unit and n-6/n-3 of egg yolk,serum superoxide dismutase(SOD)compared with CON.No significant differences were observed for Haugh unit and n-6/n-3 of egg yolk among CTC,RE50,RE100 and RE200 groups.Compared with CTC and RE50 groups,RE200 increased serum SOD activity on d 28 and 56.Compared with CON,RE supplementation decreased(P<0.05)total cholesterol(TC)level.CTC,RE100 and RE200 decreased(P<0.05)serum interleukin-6(IL-6)content compared with CON.CTC and RE200 increased jejunal m RNA expression of ZO-1 and Occludin compared with CON.The biomarkers of cecal microbiota and metabolite induced by RE 200,including Firmicutes,Eisenbergiella,Paraprevotella,Papillibacter,and butyrate,were closely associated with Haugh unit,n-6/n-3,SOD,IL-6,and TC.PICRUSt2 analysis indicated that RE altered carbohydrate and amino acid metabolism of cecal microbiota and increased butyrate synthesizing enzymes,including 3-oxoacid Co A-transferase and butyrate-acetoacetate Co A-transferase.Moreover,transcriptomic analysis revealed that RE200 improved gene expressions and functional pathways related to immunity and albumen formation in the oviductal magnum.Conclusions Dietary supplementation with 200 mg/kg RE could increase egg quality of late-p

关键词： Intestinal health Laying hens Microbiota Oviductal function Phytochemicals Production

Development and validation of a nomogram for predicting in-hospital mortality of intensive care unit patients with liver cirrhosis

在线全文

学校读者我要写书评

暂无评论

World Journal of Hepatology 2024年第4期16卷 625-639页

作者： Xiao-Wei Tang Wen-Sen Ren Shu Huang Kang Zou Huan Xu Xiao-Min shi Wei Zhang lei shi Mu-Han Lü Department of Gastroenterology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646099Sichuan ProvinceChina Nuclear Medicine and Molecular Imaging Key Laboratory The Affiliated Hospital of Southwest Medical UniversityLuzhou 646099Sichuan ProvinceChina Department of Gastroenterology Lianshui People’Hospital of Kangda College Affiliated to Nanjing Medical UniversityHuaian 223499Jiangsu ProvinceChina

BACKGROUND Liver cirrhosis patients admitted to intensive care unit(ICU)have a high mortality rate.AIM To establish and validate a nomogram for predicting in-hospital mortality of ICU patients with liver cirrhosis.METHODS We extracted demographic,etiological,vital sign,laboratory test,comorbidity,complication,treatment,and severity score data of liver cirrhosis patients from the Medical Information Mart for Intensive Care IV(MIMIC-IV)and electronic ICU(eICU)collaborative research database(eICU-CRD).Predictor selection and model building were based on the MIMIC-IV dataset.The variables selected through least absolute shrinkage and selection operator analysis were further screened through multivariate regression analysis to obtain final predictors.The final predictors were included in the multivariate logistic regression model,which was used to construct a nomogram.Finally,we conducted external validation using the eICU-CRD.The area under the receiver operating characteristic curve(AUC),decision curve,and calibration curve were used to assess the efficacy of the models.RESULTS Risk factors,including the mean respiratory rate,mean systolic blood pressure,mean heart rate,white blood cells,international normalized ratio,total bilirubin,age,invasive ventilation,vasopressor use,maximum stage of acute kidney injury,and sequential organ failure assessment score,were included in the multivariate logistic regression.The model achieved AUCs of 0.864 and 0.808 in the MIMIC-IV and eICU-CRD databases,respectively.The calibration curve also confirmed the predictive ability of the model,while the decision curve confirmed its clinical value.CONCLUSION The nomogram has high accuracy in predicting in-hospital mortality.Improving the included predictors may help improve the prognosis of patients.

关键词： Liver cirrhosis Intensive care unit Nomogram Predicting model Mortality

Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第4期23卷 1134-1149页

作者： Ping Xu Hao Li Haiyuan Li Ge Zhao Shengjie Dai Xiaoyu Cui Zhenning Liu lei shi Xiaohua Wang College of Agriculture and Forestry Science Linyi UniversityLinyi 27600China National Key Laboratory of Crop Genetic Improvement/Microelement Research Centre/Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of Agriculture and Rural Affairs/Huazhong Agricultural UniversityWuhan 430070China

Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide.As the most important organ for plants,root morphology traits(RMTs)play a key role in P absorption.To investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape accessions.The purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P stress.The expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P stress.Moreover,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed rape.Over-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed rape.Collectively,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.

关键词： genome-wide association studies(GWAS) root morphology traits(RMTs) organic phosphorus(Po) oilseed rape BnPAP17

OsMYB84,a transcriptional regulator of OsCOPT2 and OsHMA5,modulates copper uptake and transport and yield production in rice

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第2期12卷 456-469页

作者： Jingli Ding Chenchen Ji Lu Yu Chuang Wang Guangda Ding Sheliang Wang lei shi Fangsen Xu Hongmei Cai Research Center of Microelement Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070HubeiChina National Key Laboratory of Crop Genetics and Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been identified.We isolated a novel R2R3-MYB transcription factor,OsMYB84,and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2 and OsHMA expression.OsMYB84 was highly expressed in roots and anthers and induced by Cu.Overexpression of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice,facilitated Cu distribution into grain and increased grain yield.In contrast,mutation of OsMYB84 reduced Cu concentration in xylem sap.OsMYB84 bound to the promoter region of OsCOPT2 and OsHMA5 and upregulated their expression.OsCOPT2 mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.

关键词： OsMYB84 OsCOPT2 OsHMA5 Copper Rice