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ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

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动物学研究(英文) 2023年第5期44卷 848-859页

作者： Ming-Hong Sun Wen-Jie Jiang Xiao-Han Li Song-Hee lee Geun Heo Dongjie Zhou Zhi Chen Xiang-Shun Cui Department of Animal Science Chungbuk National UniversityCheongjuSouth Korea 28644Korea College of Animal Science and Technology Yangzhou UniversityYangzhouJiangsu 225009China

Activating transcription factor 6(ATF6),one of the three sensor proteins in the endoplasmic reticulum(ER),is an important regulator of ER stress-induced ***6 resides in the ER and,upon activation,is translocated to the Golgi apparatus,where it is cleaved by site-1 protease(S1P)to generate an amino-terminal cytoplasmic *** recent studies have made progress in elucidating the regulatory mechanisms of ATF6,its function during early porcine embryonic development under high-temperature(HT)stress remains *** this study,zygotes were divided into four groups:control,HT,HT+ATF6 knockdown,and HT+PF(S1P inhibitor).Results showed that HT exposure induced ER stress,which increased ATF6 protein expression and led to a decrease in the blastocyst ***,ATF6 expression was knocked down in HT embryos under microinjection of ATF6 double-stranded RNA(dsRNA).Results revealed that ATF6 knockdown(ATF6-KD)attenuated the increased expression of CHOP,an ER stress marker,and Ca2+release induced by *** addition,ATF6-KD alleviated homeostasis dysregulation among organelles caused by HT-induced ER stress,and further reduced Golgi apparatus and mitochondrial dysfunction in HT ***2 is an important downstream effector of *** showed that ATF6-KD reduced the occurrence of AIFM2-mediated embryonic apoptosis at *** together,our findings suggest that ATF6 is a crucial mediator of apoptosis during early porcine embryonic development,resulting from HT-induced ER stress and disruption of organelle homeostasis.

关键词： ATF6 Embryo High temperature ER stress Organelle homeostasis

Structural and Dielectric Properties of Dy-doped(Ba,Sr,Ca) TiO_3 Thick Films

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材料科学与工程学报 2007年第6期25卷 928-931页

作者： Hyun-Ji Noh Sung-Gap lee Sang-Man Park Sang-Eun Yun Dept.of ceramic Engineering Eng.Res.Insti.Gyeongsang National University 900 GajwaJinju-siGyeongnam 660-701Korea

Preparation and electrocatalytic activities of Pt-TiO_2 nanotubes(Ba_ 0.57Sr_ 0.33Ca_ 0.10)TiO_3 powders,prepared by the sol-gel method,were doped MnCO_3 as acceptor and Dy_2O_3 as *** powder was mixed with an organic vehicle and BSCT thick films were fabricated by the screen-printing techniques on alumina *** structural and dielectric properties of BSCT thick films were investigated with variation of Dy_2O_3 *** a result of the differential thermal analysis(DTA),exothermic peak was observed at around 670℃ due to the formation of the polycrystalline perovskite *** the BSCT thick films showed the XRD patterns of a typical polycrystalline perovskite *** average grain size of BSCT thick films decreased with increasing amount of Dy_2O_*** relative dielectric constant and dielectric loss of the BSCT thick film doped Dy_2O_3 0.1mol% were 4637.4 and 1.6% at 1kHz,respectively.

关键词：介电性能结构性能镝厚膜丝网印刷术

Tissue-specific Tregs in cancer metastasis:opportunities for precision immunotherapy

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Cellular & Molecular Immunology 2022年第1期19卷 33-45页

作者： Laura A.Huppert Michael D.Green Luke Kim Christine Chow Yan Leyfman Adil I.Daud James C.lee Division of Hematology/Oncology Department of MedicineUniversity of CaliforniaSan FranciscoSan FranciscoCAUSA Department of Radiation Oncology University of Michigan School of MedicineAnn ArborMIUSA Veterans Affairs Ann Arbor Healthcare System Ann ArborMIUSA University of California San Francisco School of MedicineSan FranciscoCAUSA Penn State College of Medicine HersheyPAUSA Parker Institute for Cancer Immunotherapy San FranciscoCAUSA

Decades of advancements in immuno-oncology have enabled the development of current immunotherapies,which provide longterm treatment responses in certain metastatic cancer ***,cures remain infrequent,and most patients ultimately succumb to treatment-refractory metastatic *** insights suggest that tumors at certain organ sites exhibit distinctive response patterns to immunotherapy and can even reduce antitumor immunity within anatomically distant tumors,suggesting the activation of tissue-specific immune tolerogenic mechanisms in some cases of therapy *** immune cells known as regulatory T cells(Tregs)are present within all tissues in the body and coordinate the suppression of excessive immune activation to curb autoimmunity and maintain immune *** the high volume of research on Tregs,the findings have failed to reconcile tissue-specific Treg functions in organs,such as tolerance,tissue repair,and regeneration,with their suppression of local and systemic tumor immunity in the context of immunotherapy *** improve the understanding of how the tissue-specific functions of Tregs impact cancer immunotherapy,we review the specialized role of Tregs in clinically common and challenging organ sites of cancer metastasis,highlight research that describes Treg impacts on tissue-specific and systemic immune regulation in the context of immunotherapy,and summarize ongoing work reporting clinically feasible strategies that combine the specific targeting of Tregs with systemic cancer *** knowledge of Tregs in the framework of their tissue-specific biology and clinical sites of organ metastasis will enable more precise targeting of immunotherapy and have profound implications for treating patients with metastatic cancer.

关键词： Tissue Tregs Immunotherapy Organ-specific tolerance Metastasis Cancer

Brain-derived neurotrophic factor mediates macrophage migration inhibitory factor to protect neurons against oxygen-glucose deprivation

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Neural Regeneration Research 2020年第8期15卷 1483-1489页

作者： Su Hwan Bae Mi Ran Yoo Ye Yeong Kim In Kyung Hong Mi Hee Kim Seung Hak lee Dae Yul Kim Department of Rehabilitation Medicine Asan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea Asan Institute for Life Sciences Asan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea

Macrophage migration inhibitory factor(MIF)is a chemokine that plays an essential role in immune system *** studies suggested that MIF protects neurons in ischemic ***,few studies are reported on the role of MIF in neurological recovery after ischemic *** purpose of this study is to identify the molecular mechanism of neuroprotection mediated by *** neuroblastoma cells were incubated in Dulbecco’s modified Eagle’s medium under oxygen-glucose deprivation(OGD)for 4 hours and then returned to normal aerobic environment for reperfusion(OGD/R).30 ng/mL MIF recombinant(30 ng/mL)or ISO-1(MIF antagonist;50μM)was administered to human neuroblastoma *** cell cultures were assigned to one of four groups:control,OGD/R,OGD/R with MIF,OGD/R with *** viability was analyzed using WST-1 *** levels of brain-derived neurotrophic factor(BDNF),microtubule-associated protein 2(MAP2),Caspase-3,Bcl2,and Bax were detected by western blot assay and immunocytochemistry in each group to measure apoptotic ***-1 assay results revealed that compared to the OGD/R group,cell survival rate was significantly higher in the OGD/R with MIF group and lower in the OGD/R with ISO-1 *** blot assay and immunocytochemistry results revealed that expression levels of BDNF,Bcl2,and MAP2 were significantly higher,and expression levels of Caspase-3 and Bax were significantly lower in the MIF group than in the OGD/R *** levels of BDNF,Bcl2,and MAP2 were significantly lower,and expression levels of Caspase-3 and Bax were significantly higher in the ISO-1 group than in the OGD/R *** administration promoted neuronal cell survival and induced high expression levels of BDNF,MAP2,and Bcl2(anti-apoptosis)and low expression levels of Caspase-3 and Bax(pro-apoptosis)in an OGD/R *** results suggest that MIF administration is effective for inducing expression of BDNF and leads to neuroprotection of neuronal cells

关键词： apoptosis brain-derived neurotrophic factor hypoxia in vitro ischemic stroke macrophage migration inhibitory factor nerve regeneration neuroprotective effect reperfusion

Challenges of Artificial Intelligence in Space Medicine

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Space(Science & Technology) 2022年第1期2022卷 363-369页

作者： Ethan Waisberg Joshua Ong Phani Paladugu Sharif Amit Kamran Nasif Zaman Andrew G.lee Alireza Tavakkoli University College Dublin School of Medicine BelfieldDublinIreland Michigan Medicine University of MichiganAnn ArborMichiganUSA Brigham and Women’s Hospital Harvard Medical SchoolBostonMassachusettsUSA Human-Machine Perception Laboratory Department of Computer Science and EngineeringUniversity of NevadaRenoRenoNevadaUSA Center for Space Medicine Baylor College of MedicineHoustonTexasUSA Department of Ophthalmology Blanton Eye InstituteHouston Methodist HospitalHoustonTexasUSA

The human body undergoes many changes during long-duration spaceflight including musculoskeletal,visual,and behavioral *** of these microgravity-induced effects serve as potential barriers to future exploration *** advent of artificial intelligence(AI)in medicine has progressed rapidly and has many promising applications for maintaining and monitoring astronaut health during ***,the austere environment and unique nature of spaceflight present with challenges in successfully training and deploying successful systems for upholding astronaut health and mission *** this article,the dynamic barriers facing AI development in space medicine are *** diverse challenges range from limited astronaut data for algorithm training to ethical/legal considerations in deploying automated diagnostic systems in the setting of the medically limited space *** to address these challenges is then discussed and future directions for this emerging field of research.

关键词： legal directions barriers

Nuclear and chloroplast genome diversity revealed by low-coverage whole-genome shotgun sequence in 44 Brassica oleracea breeding lines

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Horticultural Plant Journal 2021年第6期7卷 539-551页

作者： Sampath Perumal Nomar Espinosa Waminal Jonghoon lee Hyun-Jin Koo Boem-soon Choi Jee Young Park Kyounggu Ahn Tae-Jin Yang Department of Agriculture Forestry and BioresourcesPlant Genomics and Breeding Instituteand Research Institute of Agriculture and Life SciencesCollege of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Republic of Korea Global Institute for Food Security University of Saskatchewan421 Downey RoadSaskatoonSK S7N 4L8Canada Agriculture and Agri-Food Canada 107 Science PlaceSaskatoon S7N 0X2Canada Chromosome Research Institute Department of Life ScienceSahmyook UniversitySeoul 01795Republic of Korea Joeun Seeds.Co. LtdGoesan-GunChungcheongbuk-Do 367-833Republic of Korea Phyzen Genomics Institute 13Seongnam-daero 331beon-gilBundang-guSeongnam-siGyeonggi-do 13558Republic of Korea

Whole-genome shogun sequence(WGS)data generated by next-generation sequencing(NGS)platforms are a valuable resource for crop *** produced 5–6×WGS coverage of 44 Brassica oleracea breeding lines representing seven subspecies/morphotypes:cabbage,broccoli,cauliflower,kailan,kale,Brussels sprout,and kohlrabi to systematically evaluate the nuclear and chloroplast(Cp)diversity in the 44 *** breeding *** then exploited the impact of low-coverage NGS by evaluating nuclear genome diversity and assembly,annotation of complete chloroplast(Cp)genomes and 45 S nuclear ribosomal DNA(45S nrDNA)sequences,and copy number variation for major *** genome diversity analysis has revealed a total of 496463 SNPs and 37493 indels in the nuclear genome across the 44 ***,some SNPs showed subspecies enrichment at certain chromosomal *** assembly of complete Cp genomes contained 153361–153372 bp with 37 variants including SNPs and *** 45S nrDNA transcription unit was 5802 bp long with a total of 31 SNPs from the 44 *** phylogenetic tree inferred from the nuclear and Cp genomes coincided and clustered broccoli,cauliflower,and kailan in one group and cabbage,Brussels sprout,kale,and kohlrabi in another *** morphotypes diverged during the last 0.17 million *** Cp genome diversity reflected the unique cytoplasm of each subspecies,and revealed that the cytoplasm of many breeding lines was replaced and intermingled via inter-subspecies crosses during the breeding process *** polymorphic Cp markers provide a classification system for the cytoplasm types in ***,copy numbers of major transposable elements(TEs)showed high diversity among the 44 accessions,indicating that many TEs have become active ***,we demonstrated a comprehensive utilization of low-coverage NGS data and might shed light on the genetic diversity and evolution of diverse *** morphotypes.

关键词： Brassica oleracea subspecies Chloroplast genome Nuclear ribosomal DNA Genetic diversity Phylogeny

Biology by the numbers on the Hawaiian Islands

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Frontiers of Electrical and Electronic Engineering in China 2013年第3期8卷 221-226页

作者： Rajat Bhatnagar Russell M. Gordley Volkan Sevim Connie M. lee Center for Systems and Synthetic Biology University of California San Francisco San Francisco CA 94158 USA Department of Biochemistry and Biophysics California Institute for Quantitative Biosciences University of California San Francisco San Francisco CA 94158 USA Department of Cellular and Molecular Pharmacology University of California San Francisco San Francisco CA 94158 USA Department of Bioengineering and Therapeutic Sciences University of California San Francisco San Francisco CA 94158 USA

One could imagine it might be hard to focus on science at a beautiful location such as the famous Waikiki Beach on Oahu Island, Hawaii. Amazingly, however, that＇s just what more than 330 participants did at the First... 详细信息

关键词： Artemisinin Synthetic Biology Artemisinic Acid Craig Venter Synthetic Biology Approach

Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

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Journal of Oceanology and Limnology 2020年第2期38卷 503-508页

作者： Yeon Jung PARK Mi Nan lee Eun-Mi KIM Jung Youn PARK Jae Koo NOH Tae-Jin CHOI Jung-Ha KANG Biotechnology Research Division National Institute of Fisheries ScienceBusan 46083Republic of Korea Department of Microbiology Pukyoung National UniversityBusan 48513Republic of Korea

Korean freshwater snails of the genus Semisulcospira are widely distributed across East *** has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean ***,no microsatellite markers have been generated to study the population genetic diversity of this *** the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic *** microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in *** addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira *** identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,*** observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,*** to the analysis of the cross-species transferability of these markers,four species,***,***,***,and ***,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

关键词： Semisulcospira next-generation sequencing(NGS) microsatellite markers cross-species transferability

Long-term tribocorrosion resistance and failure tolerance of multilayer carbon-based coatings

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Friction 2022年第10期10卷 1707-1721页

作者： Hao LI Linlin LIU Peng GUO Lili SUN Jing WEI Yingrui LIU Shuyu LI Shuyuan WANG Kwang-Ryeol lee Peiling KE Aiying WANG Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Computational Science Center Korea Institute of Science and TechnologySeoul 136-791Republic of Korea

Current tribocorrosion research of metallic materials and their surface protective coatings mainly focuses on their short-term properties,with test time of 0.5‒2.0 h and a sliding distance 50‒500 m,which may significantly deviate from the practical long-term service condition and thus cause a catastrophe of marine *** this study,three carbon-based multilayer coatings(Ti/DLC,TiC_(x)/DLC,and Ti‒TiC_(x)/DLC)were deposited on S32750 substrates,and both short-term and long-term tribocorrosion behaviors were *** experimental results indicate that the coatings substantially improve the tribocorrosion resistance of the S32750 stainless *** the short-term tribocorrosion test,TiC_(x)/DLC exhibited the best tribocorrosion resistance owing to its high *** the long-term tribocorrosion test,however,Ti‒TiC_(x)/DLC coating indicated the best anti-tribocorrosion performance owing to its excellent fracture toughness together with high ***,under 5 N,Ti‒TiC_(x)/DLC can withstand a long-term test of more than 24 ***,under a higher load of 20 N,the Ti‒TiC_(x)/DLC with a corresponding sliding distance of approximately 1,728 m maintained a low friction coefficient of approximately ***,the coating was completely worn out;this is attributable to the formation of tribocorrosion products consisting of graphitized carbon and nanocrystalline Fe_(x)O_(y).

关键词： DLC multilayer structure long-term tribocorrosion failure tolerance