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枸杞叶片酚酸类物质的自毒作用分析

江苏农业学报 2024年第2期40卷 213-222页

作者：宋燕芳彭彤马少兰马彩霞高娜李凯乐张传继蒲美运纳小凡兰州大学生命科学学院甘肃兰州730000 细胞活动与逆境适应教育部重点实验室甘肃兰州730000 彭阳县第一中学宁夏固原756500 宁夏大学生命科学学院宁夏银川750021

自毒作用是植物连作障碍形成的主要原因之一,严重影响植物生长和粮食生产。前期研究结果表明,土壤微生物多样性下降、土传病害加重是枸杞园连作障碍形成的重要原因,但枸杞残体的自毒作用及其物质基础目前仍不明确。为探讨自毒物质的主... 详细信息

自毒作用是植物连作障碍形成的主要原因之一,严重影响植物生长和粮食生产。前期研究结果表明,土壤微生物多样性下降、土传病害加重是枸杞园连作障碍形成的重要原因,但枸杞残体的自毒作用及其物质基础目前仍不明确。为探讨自毒物质的主要来源,本研究对比分析了枸杞(Lycium barbarum L.)叶、茎、根等组织浸提液对枸杞幼苗生长的影响。浓度梯度试验结果表明,叶片浸提液的抑制效应高于根、老根皮及茎浸提液。向无枸杞种植历史的农田土壤中添加枸杞叶片干粉能够显著抑制枸杞幼苗生长及叶片光合作用,证实了枸杞叶片具有自毒作用。联合UPLC-QTOF-MS和GC-TOF-MS技术对生长季末期枸杞叶片进行代谢组分析,共鉴定出有机酸112种、醇类41种、氨基酸37种、醛酮类28种和糖类48种。对其中24种有机酸及衍生物的分析发现,20种有机物能够不同程度抑制枸杞幼苗初生根生长。其中水杨酸、邻苯二甲酸、对羟基苯甲酸和香豆素在浓度低至10μmol/L时仍具显著抑制效应。UPLC结果进一步显示,长期连作导致枸杞园土壤香豆素、水杨酸、苯甲酸、阿魏酸和香豆酸积累。此外,土壤总酚酸含量也随种植年限增加显著增加。这些结果表明土壤中枸杞叶片酚酸能够在长期连作下积累并诱发自毒作用,这为进一步探索枸杞连作障碍形成机制提供了研究基础。

关键词：枸杞连作障碍自毒物质叶片酚酸

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Valorization of poly-β-hydroxybutyrate(PHB)-based bioplastic waste in anaerobic digesters of food waste for bioenergy generation:reactor performance,microbial community analysis,and bioplastic biodegradation

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碳中和(英文) 2022年第1期1卷 523-536页

作者： le.Zhang To-Hung Tsui Jiahua Fu Yanjun Dai Yen Wah tong NUS Environmental Research Institute National University of Singapore1 Create WayCreate Tower#15-02Singapore 138602Singapore Energy and Environmental Sustainability for Megacities(E2S2)Phase II Campus for Research Excellence and Technological Enterprise(CREATE)1 CREATE WaySingapore 138602Singapore Department of Chemical and Biomole.ular Engineering National University of Singapore4 Engineering Drive 4Singapore 117585Singapore School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghaiChina

This study aims to investigate the significance and biodegradation pathways of PHB-based bioplastic in anaerobic digesters treating food waste,where the reactor performance of changed methane generation,bioplastic biodegradation efficiency,and bioinformatic analysis of functional microbes were *** results showed that PHB-based plastic film could be partially biodegraded in the food waste digester,and a bioaugmentation use of Alcaligenes Faecalis(AF)and Bacillus Megaterium(BM)was beneficial to largely accele.ate the degradation process through a beneficial shift of both the functional bacterial and archaeal *** community analysis indicated that the major bacterial species belonged to genera Candidatus_Cloacimonas,Rikenellaceae,and Defluviitoga,while.the dominant methanogenic archaeal species belonged to genera Methanomassiliicoccus,Methanosarcina,and *** biodegradation analysis suggested that the optimal fractions of AF and BM for PHB-based plastic degradation were 50% AF and 75% BM,respectively,which deserves further optimization and scale.up *** finding of this study would contribute to the combined management of PHB-based bioplastic with food waste for cle.n energy recovery and a greener environment.

关键词： Anaerobic digestion Biodegradable.plastic Waste management Energy recovery Bioinformatic analysis Poly-β-hydroxybutyrate(PHB)

Biomass Carbon Improves the Adsorption Performance of Gangue-Based Ceramsites:Adsorption Kinetics and Mechanism Analysis

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Journal of Renewable.Materials 2023年第12期11卷 4161-4174页

作者： Haodong Li Huiling Du le.Kang Yewen Zhang tong Lu Yuchan Zhang Lan Yang Shijie Song Colle.e of Materials Science and Engineering Xi’an University of Science and TechnologyXi’an710054China Shccig Yulin Chemical Co. Ltd.Management Committee of Yushen Industrial ZoneYulinChina

The large accumulation of coal gangue,a common industrial solid waste,causes severe environmental proble.s,and green development strategies are required to transform this waste into high-value-added *** this study,low-cost ceramsites adsorbents were prepared from waste gangue,silt coal,and peanut shells and applied to remove the organic dye methyle.e blue from *** investigated the microstructure of ceramsites and the effects of the sintering atmosphere,sintering temperature,and solution pH on their adsorption *** ceramsites sintered at 800℃under a nitrogen atmosphere exhibited the largest three-dimensional-interconnected hierarchical porous structure among the prepared ceramsites;further,it exhibited the highest methyle.e blue adsorption performance,with an adsorption capacity of 0.954 mg·g^(−1),adsorption efficiency of over 95%,and adsorption equilibrium time of 1 h at a solution pH of *** removal efficiency remained greater than 75%after five adsorption *** adsorption kinetics data were analyzed using various models,including the pseudosecond-order kinetic model and Langmuir equation,and the adsorption was attributed to ele.trostatic interactions between the dyes and ceramsites,n-interactions,and hydrogen *** prepared coal gangue ceramsites exhibited excelle.t adsorption capacities,removal rates,and cyclic stabilities,demonstrating their promising application prospects for the comprehensive utilization of solid waste and for wastewater treatment.

关键词： Porous ceramsites adsorption biological carbon kinetic analysis adsorption mechanism

Deuteration triggered downward shift of diele.tric phase transition temperature in a hydrogen-bonded mole.ular crystal

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Chinese Chemical le.ters 2022年第3期33卷 1422-1424页

作者： Bei-Dou Liang tong Jin le.Ping Miao Chao-Yang Chai Chang-Chun Fan Xiang-Bin Han Wen Zhang Jiangsu Key Laboratory for Science and Applications of Mole.ular Ferroele.trics and School of Chemistry and Chemical Engineering Southeast UniversityNanjing 211189China

Deuteration of hydrogen-bonded phase transition crystals can increase the transition temperatures due to the isotope effect. But rare example. show the opposite trend that originates from the structural changes of the hydrogen bond, known as the geometric H/D isotope effect. Herein, we report an organic crystal, diethylammonium hydrogen 1,4-terephthalate, exhibits a reversible.structural phase transition and diele.tric switching. Structural study shows the cations reside in channels formed by one-dimensional hydrogen-bonded anionic chains and undergo an order-disorder transition at around 206 K. The deuterated counterpart shows an elongation of the O…O hydrogen bond by about 0.005 A. This geometric isotope effect rele.ses the internal pressure of the anionic host on the cation guests and results in a downward shift of the phase transition temperature by 10 K.

关键词： Diele.tric phase transition Stimulus responsive material Deuteration Geometric isotope effect Downward shift of transition temperature

Increasing angiotensin-converting enzyme(ACE)2/ACE axes ratio alle.iates early pulmonary vascular remodeling in a porcine model of acute pulmonary embolism with cardiac arrest

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World Journal of Emergency Medicine 2022年第3期13卷 208-214页

作者： Hong-li Xiao Lian-xing Zhao Jun Yang Nan tong le.An Guo-xing Wang Miao-rong Xie Chun-sheng Li Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Emergency Internal Medicine the Affiliated Hospital of Qingdao UniversityQingdao 266000China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100050China

BACKGROUND:Acute pulmonary embolism(APE)with cardiac arrest(CA)is characterized by high mortality in emergency due to pulmonary arterial hypertension(PAH).This study aims to determine whether early pulmonary artery remodeling occurs in PAH caused by massive APE with CA and the protective effects of increasing angiotensin-converting enzyme(ACE)2-angiotensin(Ang)(1-7)-Mas receptor axis and ACE-Ang II-Ang II type 1 receptor(AT1)axis(ACE2/ACE axes)ratio on pulmonary artery le.ion after return of spontaneous circulation(ROSC).METHODS:To establish a porcine massive APE with CA model,autologous thrombus was injected into the external jugular vein until mean arterial pressure dropped below 30 mmHg(1 mmHg=0.133 kPa).Cardiopulmonary resuscitation and thrombolysis were delivered to regain spontaneous *** were divided into four groups of five pigs each:control group,APE-CA group,ROSC-saline group,and ROSC-captopril group,to examine the endothelial pathological changes and expression of ACE2/ACE axes in pulmonary artery with or without ***:Histological analysis of sample. from the APE-CA and ROSC-saline groups showed that pulmonary arteriole. were almost comple.ely occluded by accumulated endothelial *** blotting analysis reveale. a decrease in the pulmonary arterial ACE2/ACE axes ratio and increases in angiopoietin-2/angiopoietin-1 ratio and expression of vascular endothelial growth factor(VEGF)in the APE-CA group compared with the control *** significantly suppressed the activation of angiopoietin-2/angiopoietin-1 and VEGF in ple.iform le.ions formed by proliferative endothelial cells after *** also alle.iated endothelial cell apoptosis by increasing the B-cell lymphoma-2(Bcl-2)/Bcl-2-associated X(Bax)ratio and decreasing cle.ved caspase-3 ***:Increasing the ACE2/ACE axes ratio may ameliorate pulmonary arterial remodeling by inhibiting the apoptosis and proliferation of endothelial cells after ROSC i

关键词： Acute pulmonary embolism Cardiac arrest Early pulmonary vascular remodeling Angiotensin-converting enzyme

Microstructure and deformation mechanism of dual-phase Al_(0.5)CoCrNiFe high-entropy alloy

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Rare Metals 2023年第6期42卷 2020-2027页

作者： Yong-Gang tong Nan Tian Hong-Feng Huang Zhi-Bin Zhang Xiu-Bing Liang Xi-Xi Ji Jing-Zhong Fang Yong-le.Hu Colle.e of Automotive and Mechanical Engineering Changsha University of Science and TechnologyChangsha410114China Key Laboratory of New Processing Technology for Nonferrous Metal and Materials Guilin University of TechnologyMinistry of EducationGuilin541004China National Institute of Defense Technology Innovation Academy of Military Sciences PLA ChinaBeijing100071China

Dual-phase high-entropy alloys containing facecentered cubic(fcc) and body-centered cubic(bcc) phases achieve a combination of high strength and high ductility,which attract extensive *** with single.hase high-entropy alloys,the dual-phase structure generates more comple. deformation mechanisms such as structural transformation and interactions between grain boundaries and dislocations during *** order to understand the structural transformation of the dual-phase high-entropy alloy during deformation and its effect on mechanical properties,Al_(0.5)CoCrNiFe high-entropy alloy was prepared and its deformation mechanism was investigated by mole.ular dynamics simulations combined with *** results show that phase transformation occurred during deformation,and dislocation slip was the main deformation *** addition,there was significant dislocation pile.up at the interface between fcc and bcc phases after tensile.*** and strain rates significantly affected the mechanical properties and deformation behavior of high-entropy *** low temperature and high strain rate,the dislocation density of the alloy increases after stretching,resulting in the enhancement of tensile.strength.

关键词： Dual-phase high-entropy alloy(HEA) Microstructure Deformation behavior Dislocation

癸酸锌的合成及其对金黄色葡萄球菌和大肠杆菌的抑菌机制研究

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饲料研究 2024年第2期47卷 82-88页

作者：高蕊李柯洁周佳乐祁丽雯黄小露高雨僮乔汉桢王金荣河南工业大学生物工程学院河南郑州450001

研究旨在以癸酸与硫酸锌为原料高效制备癸酸锌并探讨其抑菌作用及机制。试验采用皂化复分解法将癸酸与硫酸锌螯合生成癸酸锌并进行结构表征;以金黄色葡萄球菌和大肠杆菌为模型,采用二倍稀释法测定其最低抑菌浓度(MIC)和最低杀菌浓度(MB... 详细信息

研究旨在以癸酸与硫酸锌为原料高效制备癸酸锌并探讨其抑菌作用及机制。试验采用皂化复分解法将癸酸与硫酸锌螯合生成癸酸锌并进行结构表征;以金黄色葡萄球菌和大肠杆菌为模型,采用二倍稀释法测定其最低抑菌浓度(MIC)和最低杀菌浓度(MBC),研究癸酸锌的抑菌活性;检测癸酸锌对金黄色葡萄球菌和大肠杆菌的细胞膜通透性、生物膜形成、DNA与蛋白质合成的影响,阐释癸酸锌的抑菌机制。结果显示,癸酸锌晶型结构好,粒径均一,对金黄色葡萄球菌和大肠杆菌均具有较强的抑菌效果,MIC分别为250、350 mg/L,对金黄色葡萄球菌抑菌作用更强。癸酸锌可通过时间依赖方式破坏金黄色葡萄球菌和大肠杆菌细胞膜通透性,抑制菌体生物膜的形成,从而发挥抑菌作用,但对大肠杆菌和金黄葡萄球菌的DNA和蛋白质合成影响不显著。研究表明,癸酸锌可通过抑制生物膜形成、破坏细胞膜通透性等途径对革兰氏阳性菌和革兰氏阴性菌表现出较强的抑菌活性,具有作为抗生素替代品应用于畜禽生产的潜力。

关键词：癸酸锌中链脂肪酸金黄色葡萄球菌大肠杆菌抑菌活性畜禽生产

Survival benefits and disparities in radiation therapy for elderly patients with pancreatic ductal adenocarcinoma

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World Journal of Gastrointestinal Oncology 2023年第1期15卷 155-170页

作者： Bi-Yang Cao Qian-Qian Wang le.Tian Zhang Chen-Chen Wu Fang tong Wei Yang Jing Wang Department of Radiation Oncology First Medical Center of Chinese PLA General HospitalBeijing 100853China Medical School of Chinese PLA Beijing 100853China

BACKGROUND Older patients represent a unique subgroup of the cancer patient population,for which the role.of cancer therapy requires special ***,the outcomes of radiation therapy(RT)in elderly patients with pancreatic ductal adenocarcinoma(PDAC)are not well-defined in the *** To explore the use and effectiveness of RT in the treatment of elderly patients with PDAC in clinical *** Data from patients with PDAC aged≥65 years between 2004 and 2018 were colle.ted from the Surveillance,Epidemiology,and End Results *** logistic regression analysis was performed to determine factors associated with RT *** survival(OS)and cancer-specific survival(CSS)were evaluated using the Kaplan–Meier method with the log-rank *** and multivariate analyses with the Cox proportional hazards model were used to identify prognostic factors for *** score matching(PSM)was applied to balance the baseline characteristics between the RT and non-RT *** analyses were performed based on clinical *** A total of 12245 patients met the inclusion criteria,of whom 2551(20.8%)were treated with RT and 9694(79.2%)were *** odds of receiving RT increased with younger age,diagnosis in an earlier period,primary site in the head,localized disease,greater tumor size,and receiving chemotherapy(all Ple.median OS:14.0 vs 11.0 mo;HR for OS:0.818,95%CI:0.768–0.872,Ple. that the survival benefits(OS and CSS)of RT were more significant in patients aged 65 to 80 years,in regional and distant stages,with no surgery,and receiv

关键词： Pancreatic ductal adenocarcinoma Elderly Radiotherapy Effectiveness Disparities Surveillance,Epidemiology,and End Results

A novel non-invasive identification of genome editing mutants from insect exuviae

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Insect Science 2022年第1期29卷 21-32页

作者： Xiao-Xiao Wang Jing Li tong-Xin Wang Yi-Nuo Yang Hai-Kang Zhang Meng Zhou le.Kang Li-Ya Wei Colle.e of Life Sciences Institute of Life Sciences and Green DevelopmentHebei UniversityBaodingHebei Province071002China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing100101China

With the wide application of genome editing in insects, a simple.and efficient identification method is urgently needed to meet the increasing demand for mutation detection. Here, taking migratory locusts as a model system, we developed a non-invasive method to accurately identify genome-edited mutants by using DNA from insect exuviae. We compared the quantity and quality of genomic DNA from exuviae in five instar hoppers and found that the 1st instar exuviae had the highest DNA yield and content, while.the 3rd instar exuviae had the best quality. Consensus genotypes were identified from genomic DNA of hoppers at different developmental stages in the same individuals. Moreover, we demonstrated that the amplification products from DNA extracted from locust exuviae are the consensus sequences with those from the hemolymph and forele. pre-tarsus. Therefore, non-invasive sample. provide the same genotyping results as minimally invasive and invasive sample. of the same individuals. Furthermore, this identification method that uses genomic DNA from exuviae can be used for early screening of positive genome-edited individuals in each generation for adult crossing. In our study, the non-invasive identification method was not only simple. and provided results earlier than existing methods, but also had a better reproducibility and accuracy. This non-invasive identification approach using genomic DNA from exuviae can be adapted to meet the growing demand for genetic analysis and will find wide application in insect genome editing research.

关键词： exuviae genome editing identification locusts molt non-invasive