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Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue

Journal of Animal Science and Biotechnology 2016年第3期7卷 403-411页

作者： Timothy G.Ramsay Margo J.Stoll le ann blomberg Thomas J.Caperna Animal Biosciences and Biotechnology Laboratory USDA/ARSBeltsville Agricultural Research CenterUSDABeltsvilieMD 20705USA

Background： Porcine adipose tissue expresses orosomucoid（ORM1） mRNA, a protein with anti-inflammatory and immunomodulatory properties. Previous research has demonstrated that porcine ORM1 can reduce insulin stimulated glucose metabolism in porcine adipose tissue in vitro. The present study was designed to examine the preweaning ontogeny of ORM1 m RNA abundance in porcine subcutaneous adipose and to determine if ORM1 can regulate m RNA abundance of inflammatory cytokines that contribute to insulin resistance in primary cultures derived from neonatal porcine subcutaneous adipose tissue. Cultures were differentiated in vitro and subsequently the adipocyte containing cultures were incubated for 24 h with 0–5000 ng porcine ORM1/m L medium. Cultures were then harvested, total RNA extracted for use in reverse transcription and the m RNA abundance of cytokine m RNA quantified by real-time ***： ORM1 m RNA abundance within neonatal adipose tissue does not change from d 1 to d 21 of age and is a very small fraction relative to liver m RNA abundance. The ORM1 m RNA level in porcine adipocytes and stromalvascular cells are similar（P 〉 0.05）. Treatment with ORM1 did not affect TNFα（tumor necrosis factor α） m RNA level（P 〉 0.05）, while interleukin 6（IL6） m RNA abundance was reduced 32 % at 1,000 ng ORM1/m L（P 〈 0.01）. However,TNFα protein content in the cell culture media was reduced by ORM1 treatment（5,000 ng/m L, P 〈 0.05）, whereas ORM1 had no detectable effect on the media content of IL6（P 〉 0.05）. The reduction of macrophage migration inhibitory factor（MIF） m RNA abundance by ORM1 was dose dependent（P 〈 0.01）. Monocyte chemotactic protein（MCP） m RNA level was reduced 27 % by 1,000 ng ORM1/m L（P 〈 0.05）.Conclusions： The data suggest that ORM1 has limited effects TNFα, IL6, MIF or MCP expression at the concentrations tested. Secondly, these cytokines do not appear to contribute to the reported insulin resistance induced by ORM1 in porcine adipose tissue in vitro as an in

关键词： Adipose Cytokines Neonate Orosomucoid Swine

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Metabolomic analysis of longissimus from underperforming piglets relative to piglets with normal preweaning growth

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Journal of Animal Science and Biotechnology 2018年第3期9卷 630-638页

作者： Timothy G.Ramsay Margo J.Stoll Amy E.Shannon le ann blomberg Animal Biosciences and Biotechnology Laboratory U.S.Department of Agriculture Agricultural Research Service

Background: Recent increases in intra-litter variability in weaning weight have raised swine production costs. A contributor to this variability is the normal birth weight pig that grows at a slower rate than littermates of similar birth weight. The goal of this study was to interrogate biochemical profiles manifested in skeletal muscle originating from slow growing(SG) and faster growing littermates(control), with the aim of identifying differences in metabolic pathway utilization between skeletal muscle of the SG pig relative to its littermates. Samples of longissimus muscle from littermate pairs of pigs were collected at 21 d of age for metabolomic ***: Birth weights did not differ between littermate pairs of SG and Control pigs(P > 0.05). Weaning weights differed by 1.51 ± 0.19 kg(P le samples by growth rate, resulting in a predictive accuracy of 81% versus random segregation(50%).Decreases in sugars in the pentose phosphate pathway(PPP) in the longissimus of SG pigs were detected(P levels of phospholipids, lysolipids, diacylglycerols, and sphingolipids(P lecules associated with muscle and collagen/extracellular matrix breakdown that are increased in the SG pig(glutamate, 3-methylhistidine and hydroxylated proline moieties; P leotide pool(P le in the slow growth in some normal birth weight piglets that contribute to inc

关键词： Growth rate Longissimus Metabolome Neonate Swine

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Metabolomic analysis of longissimus from underperforming piglets relative to piglets with normal preweaninggrowth.

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畜牧与生物技术杂志(英文版) 2018年第3期

作者： Timothy G.Ramsay Margo J.Stoll Amy E.Shannon le ann blomberg

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Correlation between Saccharomyces cerevisiae DNA in intestinal mucosal samples and anti-Saccharomyces cerevisiae antibodies in serum of patients with IBD

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World Journal of Gastroenterology 2006年第2期12卷 292-297页

作者： RC Mallant-Hent M Mooij BME von blomberg RK Linskens AA van Bodegraven PHM Savelkoul Department of Gastroenterology VU University Medical CenterAmsterdamThe Netherlands Department of Medical Microbiology and Infection Control VU University Medical CenterAmsterdamThe Netherlands Department of Immunology VU University Medical CenterAmsterdamThe Netherlands Department of Gastroenterology St Anna ZiekenhuisGeldropThe Netherlands

AIM： To investigate the correlation between ASCA and presence of mucosal S. cerevisiae DNA in a population of CD, ulcerative colitis （UC） patients and controls. METHODS： S. cerevisiae-specific primers and a fluorescent probe were designed for a 5＇ exonuclease real time PCR （TaqManTM） assay, which is a homogenous system using a fluorescent-labelled probe for the detection of PCR product in real time. We analyzed the relation of the PCR results with the ASCA findings in a group of 76 inflammatory bowel disease （IBD） patients （31 CD, 45 UC） and 22 healthy controls （HC）. RESULTS： ASCA （IgA or IgG） were positive in 19 （61%） patients with CD, 12 （27%） with UC and none of the HC. PCR amplification was inhibited and excluded from the final results in 10 （22%） UC patients, 7 （22%） CD patients, and 6 （30%） HC. In only 15 of the mucosal samples, S. cerevisiae DNA was detected by real time PCR, including 7 （29%） in CD, 7 （19%） in UC, 1 （6%） in HC. In 4 CD and in 4 UC patients, ASCA and mucosal S. cerevisiae were positive. Hucosal S. cerevisiae was present in combination with negative ASCA IgA and IgG in 3 UC, and 3 CD patients. CONCLUSION： We conclude that since the presence of S. cerevisiae in colonic mucosal biopsy specimens is very rare, ASCA is unlikely to be explained by continuous exposure to S. cerevisiae in the mucosa. Therefore, ASCA formation must occur earlier in life and levels remain relatively stable thereafter in immunological susceptible persons.

关键词： ASCA Saccharomyces cerevisiae mucosa IBD

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建构社会信用体系的法制路径探析

建构社会信用体系的法制路径探析

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作者： Marianne Freiin von blomberg 浙江大学

学位级别：硕士

2014年,国务院公开宣称,社会信用体系的建设是一项国家课题。从那时开始,一些政府指定的地区和公司已经开始试行对企业、个人以及行政主体进行评分的制度。无论从它所考量的行为上看,还是从它提供的奖励和惩戒的范围上看,这项制度都是... 详细信息

2014年,国务院公开宣称,社会信用体系的建设是一项国家课题。从那时开始,一些政府指定的地区和公司已经开始试行对企业、个人以及行政主体进行评分的制度。无论从它所考量的行为上看,还是从它提供的奖励和惩戒的范围上看,这项制度都是前所未有的。根据这项制度的要求,对于个人隐私和商业隐私将有所牺牲。这种牺牲是为了更好的公共服务和商业服务的提供,以及对于法律更为迅捷和有效的执行。尽管它可能对私人和企业产生重大的影响,在国家层面上为该制度服务的法律框架尚未建立。本文通过分析试点省份和城市的地方人大所通过的行政规章,利用政府和商业试点所得到的经验,试图解决一些社会信用体系的建立者所面临的简单问题。这种分析说明,国家层面的立法者首先不得不对“信誉度”这一术语进行清晰地定义,从而阐明何种行为将与社会信用有关,刑法的界限为何,以及国家的奖励是否构成它的一部分。其次,隐私权的保护以及对于信用主体的维护必须通过避免技术上和体系上的错误得到强调。这些错误在构建统一的社会信用体系的过程中很有可能会发生。同时,只有通过维护信用主体的利益,才能够避免在社会信用体系管理过程中可能出现的权力滥用和贪污腐败。再次,通过分析可以得知,社会信用体系的影响是如此巨大,以致于单纯颁布一部法律是无法解决目前存在的众多问题的。这样的一部法律将会涉及许多不同的领域,并与现行法相冲突。合适的做法是,对相关法律领域的一系列问题进行仔细考虑,从而通过完善的立法来保障社会信用体系。

关键词： social credit system punishment reward legislation big data pilot privacy economy regulation judiciary digitalization trustworthiness credibility sincerity

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典型重金属污染地区蔬菜中重金属含量及健康风险

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环境污染与防治 2017年第9期39卷 952-956页

作者：杨俊吕府红宋永伟张敬东 Jerry blomberg 中南财经政法大学环境与健康研究中心湖北武汉430073 中南财经政法大学环境管理与政策研究所湖北武汉430073 Department of Business Administration Technology and Social SciencesLulea University of Technology

在大冶市典型重金属污染地区,选择罗家桥街办作为污染区、茗山乡作为对照区,通过采集土壤和蔬菜样品,分析5种重金属(Cu、Zn、As、Cd、Pb)的含量,评价重金属对当地居民的健康风险,评价参数根据当地居民调查得到。结果表明,污染区土壤样品... 详细信息

在大冶市典型重金属污染地区,选择罗家桥街办作为污染区、茗山乡作为对照区,通过采集土壤和蔬菜样品,分析5种重金属(Cu、Zn、As、Cd、Pb)的含量,评价重金属对当地居民的健康风险,评价参数根据当地居民调查得到。结果表明,污染区土壤样品的5种重金属的平均值均高于背景值,对照区土壤样品的5种重金属的平均值与背景值差异不显著或低于背景值。污染区蔬菜样品的5种重金属的平均值也基本都高于对照区。因此,污染区土壤已经受到了重金属的污染,对照区土壤几乎未受到重金属的污染。健康风险评价结果表明,污染区人群面临着非致癌风险和致癌风险,而对照区健康风险较小。从年龄层看,对非致癌风险抵抗力最弱的是儿童,而对致癌风险抵抗力最弱的是成人。污染区最主要的风险元素是Cd和As。

关键词：重金属污染蔬菜健康风险评价

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蔗渣制浆造纸工艺的研究

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造纸科学与技术 1990年第1期16卷 1-17,22页

作者： Jan-Erik levlin Liisa blomberg Timo Jousimaa Ilkka Wartiovaara 王仁宇冯国基芬兰制浆造纸研究所广东省造纸研究所

用蔗渣制浆造纸的研究已经完成了。研究的目的是: ——获得有关蔗渣处理的资料 ——取得可供设计碱回收系统的资料 ——发展漂白方法,使碱法蔗渣浆有良好的抄纸性能,包括白度在内。 ——寻找以蔗渣浆为其中一种原料抄制不需涂布纸张... 详细信息

用蔗渣制浆造纸的研究已经完成了。研究的目的是: ——获得有关蔗渣处理的资料 ——取得可供设计碱回收系统的资料 ——发展漂白方法,使碱法蔗渣浆有良好的抄纸性能,包括白度在内。 ——寻找以蔗渣浆为其中一种原料抄制不需涂布纸张的最佳配比。实验工作由芬兰制浆造纸研究所进行,原料是广东省造纸研究所提供的未漂白烧碱法蔗渣浆。研究结果摘要如下: 蔗渣处理需进一步改进,如有关除髓、厂内运输、筛选、净化、测定及控制水分等设备。当然,对于木浆厂的同类设备加以改进是可以解决问题的。碱法浆废液的固形物含量比通常硬木或软木硫酸盐法的废液要低。低的结垢点意味着在蒸发黑液时会产生结垢问题。粘度非常高,在设计碱回收工场时必须给予考虑。以硫酸盐浆厂成熟的同样工艺为基础的碱回收工场也可适用于蔗渣纸浆厂。本试验发展了用于碱法蔗渣浆的现代的、低污染的四段漂白工艺O(DC)(EO)D,此法采用了氧、二氧化氯作漂白剂。用这种方法得到的白度相当高,大约为87％,而浆的粘度高。较之中国现用的典型的CEH连续三段漂所达到的白度83％有显著提高。

关键词：漂白工艺蔗渣浆硫酸盐浆碱法浆制浆造纸碱回收系统卡伯值烧碱法硫酸盐法漂白试验

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How is Methane Formed and Oxidized Reversibly When Catalyzed by Ni-containing Methyl-Coenzyme M Reductase? Theoretical Investigations

How is Methane Formed and Oxidized Reversibly When Catalyzed...

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中国化学会第十二届全国量子化学会议

作者：陈世稆 Margareta R.A.blomberg Per E.M.Siegbahn 北京理工大学化学学院 Department of Organic Chemistry Stockholm University

Methyl-coenzyme M reductase（MCR）is a nickel-containing enzyme responsible for the last step of methanogenesis in methanogenic *** is capable of catalyzing methane formation from methyl-coenzyme M（CH3-SCo M）and coenzyme B（Co B-SH）,and also its reverse reaction（anaerobic methane oxidation）.By our calculations,a previously proposed mechanism for MCR,including an organometallic methyl-Ni（ⅡI）F430intermediate,has beenruled out,and a new mechanism involving a Ni（Ⅱ）-SCo M intermediate and a methyl radical was *** possible competitive mechanisms were also investigated.

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用于HVDC晶闸管模块运行试验的新合成试验回路

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高压电器 2002年第5期38卷 1-4页

作者：盛宝良 E.Jansson A.blomberg H-O Bjarme D.Windmar ABBPowerSystemsABABBSwitchgearAB

现代晶闸管阀的高额定容量使其难以在试验室用背靠背试验回路进行试验,用合成试验回路对晶闸管阀的设计进行验证是一种替代方法。设计合成试验回路的核心在于,如何正确地再现作用在晶闸管阀上的负荷。为此,重点描述了换流阀在4种运行状... 详细信息

现代晶闸管阀的高额定容量使其难以在试验室用背靠背试验回路进行试验,用合成试验回路对晶闸管阀的设计进行验证是一种替代方法。设计合成试验回路的核心在于,如何正确地再现作用在晶闸管阀上的负荷。为此,重点描述了换流阀在4种运行状态下的电压和电流负荷。ABBPowerSystems开发了一种新的合成试验回路,正确地再现了运行中施加在HVDC晶闸管阀上的电压和电流负荷。验证试验表明,该回路的设计是成功的。

关键词： HVDC 晶闸管模块运行试验新合成试验回路直流输电

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CRISPRi screen highlights chromatin regulation to be involved in formic acid tolerance in Saccharomyces cerevisiae

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Engineering Microbiology 2023年第2期3卷 57-64页

作者： Vaskar Mukherjee Ibai lenitz Ulrika Lind Anders blomberg Yvonne Nygård Department of Life Sciences Chalmers University of TechnologyGothenburgSweden Department of Chemistry and Molecular Biology University of GothenburgGothenburgSweden Department of Biorefinery and Energy RISE Research Institutes of SwedenÖrnsköldsvikSweden

Formic acid is one of the main weak acids in lignocellulosic hydrolysates that is known to be inhibitory to yeast growth even at low *** this study,we employed a CRISPR interference(CRISPRi)strain library comprising>9000 strains encompassing>98%of all essential and respiratory growth-essential genes,to study formic acid tolerance in Saccharomyces *** provide quantitative growth estimates on formic acid toler-ance,the strains were screened individually on solid medium supplemented with 140 mM formic acid using the Scan-o-Matic *** resistant and sensitive strains were characterized in liquid medium supplemented with formic acid and in synthetic hydrolysate medium containing a combination of *** with gR-NAs targeting genes associated with chromatin remodeling were significantly enriched for strains showing formic acid *** line with earlier findings on acetic acid tolerance,we found genes encoding proteins involved in intracellular vesicle transport enriched among formic acid sensitive *** growth of the strains in syn-thetic hydrolysate medium followed the same trend as when screened in medium supplemented with formic *** sensitive to formic acid had decreased growth in the synthetic hydrolysate and all strains that had im-proved growth in the presence of formic acid also grew better in the hydrolysate *** analysis of CRISPRi strains allowed identification of genes involved in tolerance mechanisms and provided novel engineering targets for bioengineering strains with increased resistance to inhibitors in lignocellulosic hydrolysates.

关键词： Formic acid Lignocellulosic hydrolysates CRISPRi library Screening Tolerance Yeast Chromatin

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