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Impact of chronic exposure to bevacizumab on EpCAM-based detection of circulating tumor cells

Chinese Journal of Cancer Research 2015年第5期27卷 491-496页

作者： Chiara Nicolazzo Isabella Massimi lavinia v.lotti Simone vespa Cristina Raimondi Fabio Maria Pulcinelli Angela Gradilone Paola Gazzaniga Dipartimento di Medicina Molecolare Sapienza Università di Roma Dipartimento di Medicina Sperimentale Sapienza Università di Roma

Background： Circulating tumor cells （CTCs） are often undetected through the immunomagnetic epithelial cell adhesion molecule （EpCAM）-based CellSearch~ System in breast and colorectal cancer （CRC） patients treated with bevacizumab （BEv）, where low CTC numbers have been reported even in patients with evidence of progression of disease. To date, the reasons for this discrepancy have not been clarified. This study was carried out to investigate the molecular and phenotypic changes in CRC cells after chronic exposure to BEv in vitro. Methods： The human CRC cell line WiDr was exposed to a clinically relevant dose of BEv for 3 months in vitro. The expression of epithelial and mesenchymal markers and EpCAM isoforms was determined by western blotting and immunofluorescence. To evaluate the impact of EpCAM variant isoforms expression on CTC enumeration by CellSearch, untreated and treated colon cancer cells were spiked into 7.5 mL of blood from a healthy donor and enumerated by CellSearch. Results： Chronic exposure of CRC cell line to BEv induced decreased expression of EpCAM 40 kDa isoform and increased expression EpCAM 42 kDa isoform, together with a decreased expression of cytokeratins （CK）, while no evidence of epithelial to mesenchymal transition （EMT） in treated cells was observed. The recovery rate of cells through CellSearch was gradually reduced in course of treatment with BEv, being 84% , 70% and 40% at l, 2 and 3 months, respectively. Conclusions： We hypothesize that BEv may prevent CellSearch from capturing CTCs through altering EpCAM isoforms.

关键词： Circulating tumor cells （CTCs） epithelial cell adhesion molecule （EpCAM） isoform bevacizumab（BEv） colorectal cancer （CRC）

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Microcephaly prevalence after the 2015 to 2016 Zika outbreak in Tangará da Serra, Brazil: a population-based study

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Reproductive and Developmental Medicine 2022年第2期6卷 98-103页

作者： Juliana Herrero da Silva Ana Claudia Pereira Tercas-Trettel Juliano Andre Boquett Giovanny vinicius Araujo Franca Augusto Cesar Cardoso-dos-Santos Marina Atanaka Marcelo Zagonel Oliveira Anna Pires Terra viviane Karoline vivi Lucia Andreia Nunes de Oliveira Lucia Schaedler Ronaldo Fernandes Santos Alves lavinia Schuler-Faccini Prefeitura Municipal de Tangara da Serra Secretaria de SaudeVigilancia EpidemiologicaTangara da SerraBrazil Programa de Pos-Graduacao em Genetica e Biologia Molecular Universidade Federal do Rio Grande do SulPorto AlegreBrazil Universidade do Estado de Mato Grosso Tangara da SerraBrazil Programa de Pos-Graduagcao em Saude Coletiva Universidade Federal de Mato GrossoCuiabaBrazil Instituto Nacional de Genetica Medica Populacional-INaGeMP Servico de Genetica MedicaHospital de Clinicas de Porto Alegre(HCPA)Porto AlegreBrazil Programa de Pos-Graduacao em Saude da Crianga e do Adolescente Universidade Federal do Rio Grande do SulPorto AlegreBrazil Secretaria de vigilancia em Saude Ministerio da SaudeBrasiliaBrazil Laboratorio de visualizacao Avangada e Geoinformatica(vIZLab) Programa de Pos-Graduacao em Computacao AplicadaUniversidade do Vale do Rio dos SinosSao LeopoldoBrazil Programa de Pos-Graduacao em Ciencia da Informacao Universidade Federal do Rio Grande do SulPorto AlegreBrazil Clinica Santa Luzia Tangara da SerraBrazil

Objective::Prenatal infection with the Zika virus (ZIKv) can lead to congenital Zika syndrome (CZS), characterized by microcephaly and brain injury. However, there are questions regarding the prevalence of microcephaly/CZS after the ZIKv outbreak in defined geographic areas. This study aimed to identify adverse outcomes in live births of fetuses exposed in utero to the ZIKv, compared to unexposed births, as well as maternal sociodemographic, delivery, and birth characteristics.Methods::Here, we conducted a cross-sectional observational study to investigate the characteristics of all live births in the city of Tangará da Serra, Mato Grosso, Brazil, in 2016, after the outbreak of ZIKv infection in late 2015. All live births of children to women residing in the municipality of Tangará da Serra between January 1 and December 31, 2016, were evaluated, and head circumference was measured at birth and after 24 hours. Children born with microcephaly or a maternal history of confirmed or suspected prenatal ZIKv infection were evaluated by a multidisciplinary team. The outcomes of the exposed and non-exposed children were compared. Prevalence ratios and their respective 95% confidence intervals were calculated for sociodemographic, delivery, and live birth characteristics.Results::Of 1,441 live births, 106 (7.3%) were from mothers with confirmed or highly probable exposure to ZIKv. The prevalence of severe congenital microcephaly (41.7/10,000) in Tangará da Serra in 2016 was ten-fold higher than that in Latin America before 2015.Conclusion::This study may serve as a model to investigate possible outbreaks of infections in a defined geographical space in the future.

关键词： Epidemiology Live births Microcephaly Zika virus

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Study of Monte Carlo event generators for proton-proton collisions at LHC energies in the forward region

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Chinese Physics C 2019年第8期43卷 1-14页

作者： Alexandru Catalin ENE Alexandru JIPA lavinia-Elena GIUBEGA Department of Elementary Particles Physics Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering (IFIN-HH)Reactorului 30RO- 077125P.O.B. MG-6Magurele-BucharestRomania Faculty of Physics of the University of Bucharest Atomistilor 405RO-077125P.O.B. MG-11Magurele-BucharestRomania

In this paper we present a comparative study between PYTHIA, EPOS, QGSJET, and SIBYLL generators. The global event observables considered are the charged energy flow, charged particle distributions, charged hadron production ratios and v^0 ratios. The study is performed in the LHCb and TOTEM fiducial phase spaces on minimum bias simulated data samples for pp collisions at = 7 Tev , using the reference measurements from these experiments. In the majority of cases, the measurements are within a band defined by the most extreme predictions. The observed differences between the predictions and the measurements seem to be, in most part, caused by extrapolation from the central pseudorapidity region (|η| 2.5), in which the generators were mainly tuned.

关键词： phenomenological models event generators LHCb

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Performance Evaluation of the Fe-IR-120（Na）-DEHPA Impregnated Resin in the Removal Process of As（v） from Aqueous Solution

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材料科学与工程（中英文B版） 2011年第4期1卷 421-432页

作者： Mihaela Ciopec Adina Negrea lavinia Lupa Comeliu Davidescu Petru Negrea Paula Sfarloaga Faculty of Industrial Chemistry and Environmental Engineering University ＂Politehnica＂ Timisoara Piata Victoriei No.2Timisoara 300006 Romania National Institute for Research and Development in Electrochemistry and Condensed Matter Timisoara Plautius AndronescuNo. 1 Timisoara 300224 Romania

In the present paper the performance of a new functionalized material Fe-IR-120(Na)-DEHPA in the removal process of As(v) from aqueous solutions, has been investigated. The new material was obtained trough impregnation of Amberlite IR-120(Na) with di(2-ethylhexyl) phosphoric acid (DEHPA) trough the dry method. Trough impregnation of the Amberlite IR-120(Na) was obtained a resin with two functional groups which was further loaded with Fe(III) ions because of the high affinity of arsenic towards iron. The physicochemical methods of analysis (FTIR spectroscopy, EDX and SEM) approved that the impregnation with DEHPA and the iron loading, respectively, occurred. By applying the kinetic model to the experimental data it was found that the removal of As(v) ions by Fe-IR-120(Na)-DEHPA follows the pseudo-second-order rate kinetics. The linear Langmuir and Freundlich isotherm models were used to represent the experimental data and these could be well interpreted by the Langmuir isotherm. RL values between 0 and 1.0 further indicate a favorable adsorption of As(v) ions onto Fe-IR-120(Na)-DEHPA. The maximum As(v) ions uptake calculated from Langmuir model was 21.8 μg/g.

关键词： DEHPA 浸渍树脂水溶液 Langmuir模型 IR NA 搬运过程绩效评价

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Atelosteogenesis Type 2/Diastrophic Dysplasia Phenotypic Spectrum: From Prenatal to Preimplantation Genetic Diagnosis

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Open Journal of Obstetrics and Gynecology 2014年第7期4卷 399-404页

作者： Eduardo P.Mattos José Antonio A.Magalhaes Lauréane Mittaz-Crettol Ricardo Azambuja Lilian Okada Denise P.Cavalcanti Juliana Cuzzi Mariangela Badalotti Rafaella Petracco Alvaro Petracco lavinia Schüler-Faccini Maria Teresa v.Sanseverino Department of Genetics Federal University of Rio Grande do SulPorto AlegreBrazil Medical Genetics Service Hospital de Clínicas de Porto AlegrePorto AlegreBrazil School of Medicine Federal University of Rio Grande do SulPorto AlegreBrazil Division of Molecular Pediatrics Lausanne University HospitalLausanneSwitzerland Fertilitat Clínica de Reproducao Porto AlegreBrazil Skeletal Dysplasia Group Medical Genetics DepartmentFaculty of MedicineState University of CampinasCampinasBrazil Genesis Genetics Diagnosis Sao PauloBrazil

Atelosteogenesis type II (AO2) and diastrophic dysplasia (DTD) are two recessively inherited, severe skeletal dysplasias caused by mutations in the SLC26A2 gene. AO2 is an invariably lethal condition, while DTD patients may reach adult life, although both diseases have overlapping diagnostic features. Here we report a patient with an intermediate phenotype between AO2 and DTD and present the successful application of preimplantation genetic diagnosis (PGD) in this situation. Sequencing of SLC26A2 alleles in the infant identified two compound heterozygous mutations, p.Arg178Ter and p.Arg279Trp, of paternal and maternal origin, respectively. At request from the parents, PGD was developed by haplotype mapping of parental SLC26A2 alleles in eleven five-day embryos. Transference to the mother was attempted twice, finally resulting in pregnancy and delivery of a healthy baby. This exemplifies the utility of PGD for inherited lethal conditions with a significant risk of recurrence, and highlights the importance of accurate diagnosis of skeletal dysplasias with prenatal manifestation.

关键词： Atelosteogenesis Type 2 Diastrophic Dysplasia Preimplantation Genetic Diagnosis Prenatal Diagnosis Skeletal Dysplasia

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Cytoreductive surgery and HIPEC after neoadjuvant chemotherapy for advanced epithelial ovarian cancer

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World Journal of Obstetrics and Gynecology 2013年第4期2卷 167-175页

作者： Marco lotti Luisa Maria Busci Luca Campanati Fausto Catena Federico Coccolini Naoual Bakrin Pierandrea De Iaco Giorgio Ercolani Giuseppe Grosso Michele Pisano Elia Poiasina Diego Rossetti Martina Rossi Claudio Zamagni Paolo Bertoli Antonio Daniele Pinna Luigi Frigerio Luca Ansaloni Unit of General Surgery 1 Papa Giovanni XXIII Hospital 24128 Bergamo Italy Unit of Gynecology and Obstetrics Papa Giovanni XXIII Hospital 24128 Bergamo Italy Unit of General Surgery St.Orsola-Malpighi University Hospital 40138 Bologna Italy Department of Gynecology Lyon Sud Hospital69310 Pierre Benite France Unit of Gynecology and Obstetrics St.Orsola-Malpighi University Hospital V40138 Bologna Italy Medical Oncology St.Orsola-Malpighi University Hospital 40138 Bologna Italy

AIM: To reduce postoperative complications and to make possible an optimal cytoreduction, neoadjuvant chemotherapy(NACT) followed by interval debulking surgery has been applied with encouraging results. METHODS: Between December 2009 and February 2012, patients with stage ⅢC-Ⅳ epithelial ovarian cancer(EOC) underwent diagnostic laparoscopy, to assess the feasibility of optimal debulking surgery. The modifi ed Fagotti score was applied to assess the feasibility of resection with zero residual tumor. Patients who were not candidate for upfront debulking surgery were submitted to NACT, then reassessed according to the RECIST 1.1 criteria and submitted to cytoreductive surgery(CRS) and hyperthermic intraperitoneal chemotherapy(HIPEC) if they showed clinical response or stable disease. The remaining cycles of adjuvant systemic chemotherapy(ASCT) were administered postoperatively, to complete 6 cycles of systemic chemotherapy.RESULTS: Nine patients were included. Clinical response to NACT was complete in 3 patients and partial in 5 patients; one patient had stable disease. All patients underwent CRS resulting in CC0 disease prior to HIPEC. Average operative time was 510 min. Average intensive care unit stay was 2 d. Average postoperative hospital stay was 25 d. No postoperative mortality was observed. One patient experienced pelvic abscess. One patient refused ASCT. The remaining 8 patients started ASCT. Average time to chemotherapy was 36 d. All patients are alive, with an average follow up of 11 mo. Eight patients are disease-free at follow up.CONCLUSION: HIPEC after CRS for advanced EOC is feasible with acceptable morbidity and mortality. NACT may increase the chance for achieving complete cytoreduction. Phase 3 studies are needed to determine the effects of HIPEC on survival.

关键词：辅助化疗治疗方法临床分析腹腔镜

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2015年巴西寨卡病毒感染与小头畸形之间可能存在的关联

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上海预防医学 2017年第1期29卷 82-84页

作者： lavinia Schuler-Faccini Erlane M.Ribeiro Ian M.L.Feitosa Dafne D.G.Horovitz Denise P.Cavalcanti AndréPessoa Maria Juliana R.Doriqui Joao Ivanildo Neri Joao Monteiro de Pina Neto Hector Y.C.Wanderley Mirlene Cernach Antonette S.El-Husny Marcos v.S.Pone Cassio L.C.Serao Maria Teresa v.Sanseverino 朱秋丽(译) 陆仁敏(校) Universidade Federal do Rio Grande do Sul Brazil Hospital Infantil Albert Sabin FortalezaCEBrazil Universidade Federal de Pernambuco Brazil Fundaqao Oswaldo Cruz Rio de JaneiroBrazil University of Campinas Sao PauloBrazil Hospital Infantil Juvencio Mattos MaranhaoBrazil Universidade Potiguar Rio Grande do NorteBrazil University of Sao Paulo Ribeirao PretoBrazil Secretaria de Estado da Saude do Espirito Santo Brazil Universidade Federal de Sao Paulo Brazil Centro Universitario do Estado do Para Brazil Universidade do Estado do Rio de Janeiro Brazil Hospital de Clinicas de Porto Alegre Brazil Brazilian Medical Genetics Society-Zika Embryopathy Task Force 不详

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,... 详细信息

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,在她们的羊水中也检测出寨卡病毒RNA。

关键词：小头畸形病毒感染报道数量登革热病毒产前超声检查胎儿畸形西寨黄病毒属病毒流行先天性异常

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