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作者： Claudia Kaiser Ulrich Wernery Jorg Kinne laurie Marker Annette Liesegang Central Veterinary Research Laboratory DubaiUnited Arab Emirates Institute of Animal Nutrition Vetsuisse Faculty ZurichZürichSwitzerland Cheetah Conservation Fund Farm Elandsvreugde#367OtjiwarongoNamibia Center for Applied Biotechnology and Molecular Medicine University of ZurichZürichSwitzerland

Neurological signs like ataxia and hind limb paresis have often been reported in cheetahs (Acinonyx jubatus), lions (Panthera leo) and snow leopards (Panthera unica). As a cause, copper and Vitamin A deficiencies have been discussed. Many cases were seen in cheetahs and lions in the United Arab Emirates (UAE) within the last years. The aim of this study was to find correlations between nutrition, serum, and tissue levels, focusing on copper and Vitamin A. Blood and tissue samples of affected and unaffected animals were analyzed at the Central Veterinary Research Laboratory in Dubai, UAE. Animals were split into three different groups (A, B and C) according to their diets. Minerals were determined in serum, tissue, food and water samples, and serum was additionally analyzed for Vitamin A and E. Liver, kidney and spinal cord samples were taken for histopathological investigations. Mean serum copper and liver copper levels of animals fed pure chicken muscle meat without supplements were significantly lower (0.41 ± 0.71 μM/L;2.16 ± 0.95 ppm wet weight) than in animals fed a whole carcass prey diet (12.16 ± 3.42 μM/L;16.01 ± 17.51 ppm wet weight) (p < 0.05). Serum Vitamin A and E levels were highest in animals fed whole carcass prey diets (1.85 ± 0.68;27.31 ± 5.69 μM/L). Liver zinc concentrations were highest in animals fed pure chicken meat only (43.75 ± 16.48 ppm wet weight). In histopathology, demyelination of the spinal cord was found in all of the affected animals and most commonly when fed a diet based on poultry without supplements.

关键词： Ataxia Cheetah(Acinonyx jubatus) Copper Lion(Panthera leo) Vitamin A

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Automated Detection and Quantification of Prostate Cancer in Needle Biopsies by Digital Image Analysis

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Open Journal of Pathology 2014年第3期4卷 138-150页

作者： Vamsi Parimi laurie J. Eisengart Ximing J. Yang Department of Pathology Northwestern University Chicago IL USA Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Chicago IL USA

Introduction: Triple immunohistochemical (IHC) stains including antibodies specific for alpha-methylacyl-CoA-racemase and basal cell markers have been a valuable aid in accurate identification of prostate carcinoma. However, accurate quantification of minuscule areas of prostate carcinoma in biopsy specimens can often be a challenge. Here we assessed the diagnostic value and quantitative use of automated digital image analysis on triple IHC stained prostate needle biopsies. Methods: Twelve cases of prostate needle biopsy material including 75 needle cores were stained with triple-antibody cocktail (P504S + 34βE12 + p63). Slides were digitally scanned with the APERIO digital image analyzer and evaluated with the GENIE pattern and color recognition digital image analysis that we developed. A slide with known areas of adenocarcinoma, high grade prostatic intraepithelial neoplasia (PIN), benign glands and stroma was used as a training set for the automated digital image analysis platform. Results: Among 75 needle biopsy cores, 19 (25.33%) contained adenocarcinoma by histology. Digital image analysis recognized adenocarcinoma in 95% of these needle biopsies. The average area of the needle biopsy was 7.63 mm2 and overall the average area of tumor was 0.196 mm2. The smallest area of tumor recognized by the program was 0.0022 mm2 (0.0363% of the core) and the largest was 0.62 mm2 (8.17% of the core) among needle core biopsies. False positives resulted from areas of high grade PIN with patchy basal cells. The false negative was caused by uneven AMACR staining in one area of adenocarcinoma. Digital recognition of areas of interest was improved by three successive image analysis training which increased the sensitivity and specificity from 83% and 89% to 90% and 93%, respectively. Conclusions: Digital image analysis in concert with IHC triple staining may be useful for accurate detection and quantitative analysis of small foci of prostatic adenocarcinoma. Defining methods to i

关键词： Prostatic Adenocarcinoma Needle Biopsy Alpha-Methylacyl-CoA-Racemase P504S Basal Cell Immunohistochemistry Automated Digital Image Analysis

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北极星英语系列教程:听说(基础)教学参考书影印

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丛书名：北极星英语系列教程

2003年

作者： [美]laurie Frazier [美]Robin Mills[编]

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先天性心脏病实践指南

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作者： laurie R. Armsby[等]原

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中文版Excel 97 轻松入门

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丛书名：当代计算机职业培训系列教程

1997年

作者：（美）（L.佩里）laurie Perry 焦爱泉等译

本书介绍了Excel 97的基本知识及基本操作技术。

ISBN: (纸本)7111057562

本书介绍了Excel 97的基本知识及基本操作技术。

关键词：电子表格系统职业教育窗口软件教材

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Remote Sensing of Aquatic Coastal Ecosystem Processes 2006

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丛书名： Remote Sensing and Digital Image Processing

2006年

作者： laurie L. Richardson Ellsworth F. LeDrew

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投资经济学

投资经济学

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1977年

作者： J.laurie Carr 赖德良译

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Life after stroke in Appalachia

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International Journal of Nursing Sciences 2017年第2期4卷 105-111页

作者： laurie Theeke A.Noelle Lucke-Wold Jennifer Mallow Patricia Horstman West Virginia University School of Nursing Health Sciences SouthPO Box 9620MorgantownWV 26506-9620United States West Virginia University HealthCare 1 Medical Center DriveMorgantownWV 26506United States

Objective: The purpose of this study was to describe the structure of meaning in the experience of surviving stroke for adults living in ***: This qualitative phenomenological study includes a sample of 6 adult survivors of ischemic stroke who were discharged from either a community or university hospital to home in the Appalachian region. Data was collected through semi-structured interviews, transcribed, and analyzed thematically by two investigators. The explicated themes were verified by the survivors as representative of their experience. Results: Five main themes emerged: 1) Frustration with new physical and functional impairment, 2) Negative emotions including anger, guilt, loneliness, and depression 3) Need for accessible support, 4) Longing for home during recovery and, 5) Stepping forward after stroke which included sub-themes of perseverance, acceptance, and retraining. Anger was described as contributing to delayed recover and emotional lability was described as a source of anger. The familiarity of home was viewed as key to reestablishing control over one's life. Survivors described how they developed perseverance to move forward and emphasized that willingness to participate in retraining led to adapting to impairments. Acceptance was described as letting go of prior expectations of self and others so one could live in the present.

关键词： qualitative establishing stroke

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Development of a Real-Time PCR Approach for the Specific Detection of Prunus mahaleb

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Food and Nutrition Sciences 2016年第8期7卷 703-710页

作者： Malcolm Burns Michael Walker Timothy Wilkes laurie Hall Kirstin Gray Gavin Nixon Science and Innovation LGC Ltd. Teddington England

In 2015 a number of cumin spice products were withdrawn from the international market because of the suspected presence of almond, a known allergen from the Prunus genus. However, immunoassay results were unable to provide unequivocal evidence of the Prunus species present, because of significant cross-reactivity with other species within the Prunus genus. A novel real-time PCR assay was developed for the specific detection of Prunus mahaleb DNA, a species known to be capable of causing false positives in almond immunoassays. The assay was developed based on available DNA sequence information from the Internal Transcribed Spacer (ITS) region, and tested against representative species within the Prunus genus to ensure no cross-reactivity. Results showed that mahaleb DNA was detected in a cumin spice product subject to the earlier international recalls, which could not be unequivocally identified using immunoassay approaches alone. This short report details preliminary results from the application of this assay, and will be of interest to analytical laboratories involved in trace detection of ingredients in support of relevant food labelling legislation.

关键词： Prunus Almond Mahaleb Herbs and Spices Real-Time PCR

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