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High voltage electrical treatments can eco-efficiently promote the production of high added value peptides during chymotryptic hydrolysis of β-lactoglobulin

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Food Bioscience 2022年第3期47卷 197-210页

作者： Rock-Seth Agoua laurent bazinet Jacinthe Thibodeau Eugène Vorobiev Nabil Grimi Sergey Mikhaylin Institute of Nutrition and Functional Foods (INAF) Dairy Research Center (STELA)Food Science DepartmentUniversité LavalQuébecQCCanada Laboratory of Food Sustainability (EcoFoodLab) Food Science DepartmentUniversité LavalQuébecQCCanada Laboratoire de Transformation Alimentaire et Procédés Électro-Membranaires (LTAPEM) Food Science DepartmentUniversité LavalQuébecQCCanada University of Technology of Compiègne Laboratory of Agro-industrial technologiesCompiègneFrance

Chymotryptic hydrolysis of β-lactoglobulin (β-lg) leads to the release of a wide range of biofunctional ***,the proteolysis of native β-lg is limited due to its compact globular structure,thus ending with low yields of *** our previous study showed that eco-efficient High Voltage Electric Treatments (HVET),namely Pulsed Electric Fields (PEF) and Electric Arc (ARC),of β-lg substantially improved its enzymatic hydrolysis,no information regarding the nature of released peptides is available up to ***,the time-dependent release of peptides and the dynamics of this release were discussed in the current *** analysis of the final hydrolysates showed that chymotrypsinolysis of HVET samples released 19% more peptides with higher concentration than the native and preheated ***,1-min HVET were found to be more efficient than 10-min ones as they led to the release of bioactive peptides (even without chymotrypsin) with higher eco-efficiency scores.

关键词： β-lactoglobulin Peptides High-voltage electrical treatments Eco-efficiency Chymotrypsin Hydrolysis.

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Production of antihypertensive and antidiabetic peptide fractions from quinoa(Chenopodium quinoa Willd.)by electrodialysis with ultrafi ltration membranes

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Food Science and Human Wellness 2022年第6期11卷 1650-1659页

作者： Adrián González-Muñoz Marion Valle Rotimi E.Aluko laurent bazinet Javier Enrione Biopolymer Research and Engineering Lab(BiopREL) School of Nutrition and DieteticsFaculty of MedicineUniversidad de los AndesMonseñorÁlvaro del Portillo 12455Chile Centre for Biomedical Research and Innovation(CIIB) Universidad de los AndesChileMonseñorÁlvaro del Portillo 12455Chile Institute of Nutrition and Functional Foods(INAF) UniversitéLavalQuébec QC G1V 0A6Canada Department of Anatomy and Physiology Faculty of MedicineLaval Hospital Research CenterUniversitéLavalQuébec QC G1V 4G5Canada Department of Food and Human Nutritional Sciences University of ManitobaWinnipeg MB R3T 2N2Canada Department of Food Sciences Laboratoire de Transformation Alimentaire et ProcédésÉlectro Membranaire(LTAPEMLaboratory of Food Processing and Electro-Membrane Processes)UniversitéLavalQuébec QC G1V 0A6Canada

Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes(EDUF)have gained attention since it can fractionate in terms of their charge and molecular *** is a pseudo-cereal highlighted by its high protein content,amino acid profile and adapting growing *** present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic *** data showed the production of peptides ranging between 0.4 and 1.5 k ***(CQPF)(3.01%),anionic(AQPF)(1.18%)and the electrically neutral fraction quinoa protein hydrolysate(QPH)-EDUF(~95%)were ***-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF(17%and 11%)indicating potential antidiabetic *** antihypertensive effect studied in-vivo in spontaneously hypertensive rats(SHR),showed a decrease in systolic blood pressure in presence of the fractionated peptides,being 100 mg/kg a dose comparable to Captopril(positive control).These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide ***,further characterization is needed towards peptide sequencing,their respective role in the metabolism and scaling-up production using EDUF.

关键词： Quinoa Electrodialysis with ultrafiltration membranes Bioactive peptides Antihypertensive Antidiabetic

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Bioactivity of mackerel peptides on obesity and insulin resistance,an in-vivo study

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Food Bioscience 2022年第3期47卷 394-402页

作者： Soheila Abachi Hokmabadinazhad Jean-Philippe Songpadith Vanessa P.Houde Geneviève Pilon Ismail Fliss André Marette laurent bazinet Lucie Beaulieu Institute of Nutrition and Functional Foods (INAF) Université LavalQuebecQuebecCanada Department of Food Science Faculty of Agricultural and Food SciencesUniversité LavalQuebecQuebecCanada Department of Medicine Faculty of MedicineCardiology Axis of the Quebec Heart and Lung InstituteQuebecQuebecCanada

Of many metabolic irregularity risk factors,glucose intolerance,insulin resistance,and abdominal obesity are the main *** risk of vascular diseases and cancers is rather high in such individuals therefore its treatment and prevention are equally important for the health of *** including fish biopeptides have frequently shown to prevent and or ameliorate these risk *** our earlier studies,Atlantic mackerel hydrolysate and its fractions showed promising effects on immunity and ***,therefore,in the current study,aimed to examine the effects of mackerel hydrolysate along its cationic peptides,isolated at pH3 using electrodialysis with ultrafiltration membrane,on metabolic disorder factors in diet-induced insulin resistant obese *** were fed the chow and or the hypercaloric diet (high fat high sucrose) for the duration of *** the hypercaloric fed insulin resistant obese mice were gavaged daily either the vehicle and or the test material at the dosage of 208 mg of sample protein per kg mice body *** to our observations,mackerel peptides could not effectively modulate food intake,weight gain,adiposity,plasma glucose and insulin concentrations,hepatic cholesterol,and triglyceride ***,obesity and metabolic impairments could not be averted by the treatments in insulin resistant obese *** conclusion,anti-hypertensive,and immunomodulatory attributes of mackerel peptides at the tested concentrations do not seem to be correlated with its metabolic syndrome benefiting *** presume a general statement about the metabolic syndrome benefiting potency of Atlantic mackerel's biopeptides further research is warranted.

关键词： Atlantic mackerel Bioactivity Cationic peptides Diabetes Obesity

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