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Salmonella enterica Flagellin Is Recognized via FLS2 and Activates PAMP-Triggered Immunity in Arabidopsis thaliana

Molecular Plant 2014年第4期7卷 657-674页

作者： Ana Victoria Garcia Amelie Charrier Adam Schikora Jean Bigeard Stephanie Pateyron Marie-Ludivine de Tauzia-Moreau Alexandre Evrard Axel Mithofer Marie laure martin-Magniette Isabelle Virlogeux-Payant Heribert Hirt Unite de Recherche en Genomique Vegetale (URGV) UMR INRA/CNRS/Universite d'Evry Val d'Essonne 91057 Evry France Institute for Plant Pathology and Applied Zoology Research Centre for BioSystems Land Use and Nutrition JL University Giessen Heinrich-Buff-Ring 26-32 35392 Giessen Germany Transcriptomic Platform Unite de Recherche en Genomique Vegetale (URGV) 91057 Evry France Department of Bioorganic Chemistry Max Planck Institute for Chemical Ecology Hans-Knoll-Str. 8 07745 Jena Germany INRA UMR 518 MIA 75005 Paris France AgroParisTech UMR 518 MIA 75005 Paris France INRA UMR 1282 infectiologie et Sante Publique 37380 Nouzilly France

Infections with Salmonella enterica belong to the most prominent causes of food poisoning and infected fruits and vegetables represent important vectors for salmonellosis. Recent evidence indicates that plants recognize S. enterica and raise defense responses. Nonetheless, the molecular mechanisms controlling the interaction of S. enterica with plants are still largely unclear. Here, we show that flagellin from S. enterica represents a prominent pathogenassociated molecular pattern （PAMP） in Arabidopsis thaliana, which induces PAMP-triggered immunity （PTI） via the recognition of the fig22 domain by the receptor kinase FLS2. The Arabidopsis fls2 mutant shows reduced though not abolished PTI activation, indicating that plants rely also on recognition of other S. enterica PAMPs. Interestingly, the S. enterica type III secretion system （T3SS） mutant prgH- induced stronger defense gene expression than wild-type bacteria in Arabidopsis, suggesting that T3SS effectors are involved in defense suppression. Furthermore, we observe that S. enterica strains show variation in the fig22 epitope, which results in proteins with reduced PTI-inducing activity. Altogether, these results show that S. enterica activates PTI in Arabidopsis and suggest that, in order to accomplish plant colonization, S. enterica evolved strategies to avoid or suppress PTI.

关键词： Salmonella enterica Arabidopsis thaliana flagellin FLS2 immunity.

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Unmasking xenolithic eclogites:Progressive metasomatism of a key Roberts Victor sample

Unmasking xenolithic eclogites:Progressive metasomatism of a...

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中国科学院地质与地球物理研究所2014年度（第14届）学术年会

作者： Jin-Xiang Huang William L.Griffin Yoann Gréau Norman J.Pearson Suzanne Y.O'Reilly John Cliff laure martin State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChina Academy of Sciences Australian Research Council Centre of Excellence for Core to Crust Fluid Systems/GEMOC Department of Earth and Planetary SciencesMacquarie University Centre for Microscopy Characterisation and AnalysisUniversity of Western Australia

Most eclogite xenoliths from the Roberts Victor kimberlite(South Africa)have been metasomatized by melts/fluids in the carbonatitic–kimberlitic spectrum,and these can be used to define a progressive metasomatic *** stages of this progressive metasomatism have been recognized within one sample(RV07-17),using petrography,major-and trace-element compositions,and Sr-and O-isotope *** zones in RV07-17 are distinguished in terms of the compositions of their *** Zone 1 to Zone 4,the microstructure becomes less equilibrated;secondary minerals and fluid inclusions become abundant;the pyrope content of the garnets increases;and clinopyroxene shows progressive enrichment in Mg *** garnets of Zone 1 have flat REE patterns from Lu to Sm,with a strong depletion in the *** Zone 4,the relative abundance of the MREE of the garnets drops significantly,giving smoother patterns.A large relict clinopyroxene grain in Zone 1 shows a strong depletion in the LREE,but the LREE/MREE of the recrystallized clinopyroxene increases from Zone 1 to Zone *** Zone 1 to4,87Sr/86Sr in clinopyroxene increases along with Sr content andδ18O of the garnet decreases from 8.2 to 5.7‰as the Mg O content ***-element data imply that the fluids that metasomatized RV07-17 were closer to carbonatitic than kimberlitic in *** from the one relict clinopyroxene,all original compositional information in this xenolith has been swept away,making it difficult to define the *** know the origin of the xenolithic eclogites,and to use them as evidence for different geodynamic/tectonic scenarios,the least metasomatized samples must be studied;unfortunately these make up a tiny proportion of the widely studied xenolith population at Roberts Victor.

关键词： Eclogites Xenoliths Mantle metasomatism Kaapvaal craton

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Expérience des parents faceàl’accompagnement pédiatrique en HAD:étude exploratoire qualitative Parents’Experience of Pediatric Home Care:A Qualitative Exploratory Study

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Psycho-Oncologie 2024年第2期18卷 137-146页

作者： laure Salomé Jérémy martin Camille Baussant-Crenn Cécile Flahault Institut de psychologie UniversitéParis CitéBouologne-Billancourt92774France Fondation Oeuvre de la Croix Saint-Simon Service d’hospitalisationàdomicileParis75019France Laboratoire de Psychopathologie et Processus de SantéUR4057 UniversitéParis CitéBoulogne-Billancourt92774France

Aim:To investigate parents’experience of home care for children in oncology in order to identify the benefits,expectations,and possible difficulties *** and methods:A qualitative method was adopted,with non-directive interviews conducted with nine participants,then analyzed using thematic content ***:The main recurring themes in the discourse include:(1)relief,(2)positive representation of home,(3)positive representation of home care,and(4)negative representation of home *** these parents,home care appears to be positive overall,even though the introduction of home care is often reported as a source of stress for ***:These results must be interpreted with caution,but they do support previous research and highlight certain recommendations for further improving this experience,particularly for parents,but also for the sick child and the family.

关键词： Home care parents oncology pediatric children

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Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections

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Microsystems & Nanoengineering 2019年第1期5卷 100-111页

作者： Daniel Migliozzi Benjamin Pelz Diego G.Dupouy Anne-laure Leblond Alex Soltermann martin A.M.Gijs Laboratory of Microsystems Ecole Polytechnique Federale de Lausanne1015 LausanneCHSwitzerland Lunaphore Technologies SA EPFL Innovation ParkBuilding C1015 LausanneCHSwitzerland Universitatsspital Zürich Schmelzbergstrasse 128091 ZürichCHSwitzerland

Because of the close interaction between tumors and the immune system,immunotherapies are nowadays considered as the most promising treatment against *** order to define the diagnosis and the subsequent therapy,crucial information about the immune cells at the tumor site is ***,different types or activation status of cells may be indicative for specific and personalized ***,we present a quantitative method to identify ten different immuno-markers in the same tumor cut section,thereby saving precious samples and enabling correlative analysis on several cell families and their activation status in a tumor microenvironment *** designed and fabricated a microfluidic chip with optimal thermomechanical and optical properties for fast delivery of reagents on tissue slides and for fully automatic imaging by integration with an optical *** multiplexing capability of the system is enabled by an optimized cyclic immunofluorescence protocol,with which we demonstrated quantitative sequential immunostaining of up to ten biomarkers on the same tissue ***,we developed high-quality image-processing algorithms to map each cell in the entire *** proofof-concept analyses,we identified coexpression and colocalization patterns of biomarkers to classify the immune cells and their activation *** to the quantitativeness and the automation of both the experimental and analytical methods,we believe that this multiplexing approach will meet the increasing clinical need of personalized diagnostics and therapy in cancer pathology.

关键词： fluid thereby mapping

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Relationship between Adiposity, Low-Density Lipoprotein Particles Size and Cardiovascular Risk among Adult Obese Cameroonians

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Open Journal of Epidemiology 2022年第2期12卷 185-206页

作者： Jacob Tchinda Nkougni Françoise Raissa Ntentie martin Fonkoua Boris Gabin Kingue Azantsa Guy Roussel Takuissu Nguemto Evelyn Ngwa Lumngwena Judith laure Ngondi Julius Enyong Oben Laboratory of Nutrition and Nutritional Biochemistry Department of Biochemistry University of Yaounde 1 Yaounde Cameroon Department of Earth and Life Sciences Higher Teachers’ Training College University of Maroua Maroua Cameroon Centre for Food Food Security and Nutrition Research (CRASAN) Institute for the Medical Research and Medicinal Plant Studies (IMPM) Ministry of Scientific Research and Innovation Yaoundé Cameroon School of Clinical Medicine University of Witwatersrand Johannesburg South Africa Centre for the Study of Emerging and Re-Emerging Infections (CREMER) Institute for the Medical Research and Medicinal Plant Studies (IMPM) Ministry of Scientific Research and Innovation Yaoundé Cameroon

Background: Despite the evidence about the increasing prevalence of dyslipidemia among adult obese Cameroonians, little is known about the Low-Density Lipoprotein (LDL) particles which influence lipid metabolism and affect cardiovascular status. The present study aims to assess the relationship between adiposity, LDL particles size and cardiovascular risk (CVR) among adult obese Cameroonians. Methods: A cross-sectional study was conducted from September 2015 to March 2016 on apparently healthy adults (n = 1006), aged 20 - 70 years and living in the West and North-West regions of Cameroon. Anthropometric measurements, blood pressure (BP), fasting blood glucose (FBG) and lipid profile markers were analyzed and LDL particle phenotypes (LDL phenotype A;LDL phenotype I;LDL phenotype B) were characterized using small, dense LDL-cholesterol (sdLDL-c) levels. Abdominal fat accumulation (AFA) was defined as waist circumference (WC) ≥ 88 cm (men) and ≥90 cm (women) and the CVR was assessed using Framingham score method. Results: In the overall population, 36.6% were overweight, 33.1% were obese and 69.1% were overweight/obese with AFA. The prevalence of LDL phenotype B was 19.8%, 37.5% and 42.8% respectively in normal-weight, overweight and obese. Among the obese, sdLDL and triglycerides levels correlated significantly with WC (r = 0.768;p Conclusion: Among obese Cameroonians, anthropometric markers of adiposity (BMI and WC) were strongly correlated to LDL phenotype B which was associated with high CVR dependently of AFA. SdLDL particles could exacerbate the CVR in obese Cameroonians subjects.

关键词： Obese Cameroonians LDL Particle Size Adiposity Markers Cardiovascular Risk

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Assessment of Domestic Water Sources Safety: Application of the Micro Biological Survey Method and Microbiological Profile of the Contaminating Bacteria

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Journal of Water Resource and Protection 2021年第5期13卷 350-361页

作者： Sali Ben Béchir Adogaye Mabvouna Biguioh Rodrigue Nkamedjie Pete Patrick Martial Andrillene laure Deutou Wondeu Sanou Sobze martin Jean Blaise Kemogne Carla Montesano Colizzi Vittorio Department of Biology Faculty of Sciences University of Rome “Tor Vergata” Rome Italy Evangelical University of Cameroon Mbouo-Bandjoun Cameroon Faculty of Medicine and Pharmaceutical Sciences University of Dschang Dschang Cameroon

Water is essential for human life and it’s expected to be clean and safe. In Cameroon, government efforts to improve access to drinking water are mitigated. Many households are suffering from chronic water shortages leading to the majority of people using doubtful water sources. This study was carried out in accordance with guidelines produced by the STARBIOS2-EU funded project on Responsible Research and Innovation and aimed to assess the microbiological quality of water samples collected from domestic water sources in 3 divisions of the West region of Cameroon. 22 water samples from well water, stream, water pump and river were aseptically collected. At each sampling point, 50 mL of sample was taken aseptically and immediately transported to the laboratory for analysis. The detection of Coliforms bacteria was done using Micro Biological Survey (MBS) method. 1 mL of each sample was inoculated in the Coliforms MBS (Coli MBS) vial initially rehydrated with 10 mL of sterile distilled water. The Coli MBS vials were closed, shaken for homogenization, and then incubated at 37°C. From the initial red color of the Coli MBS vials, changes in color to yellow of the reaction vials were monitored at three different time intervals (12 h, 19 h and 24 h), corresponding to three levels of bacterial concentration. All positives samples on MBS method were selected to determine the presence of E. coli Loop full broths of Coli MBS vials were taken;streaked into the eosin methylene blue (EMB) agar plate and incubated at 37°C for 24 hours. E. coli was confirmed by observing green metallic sheen on EMB agar plate. The biochemical indole, methyl red, Voges-Proskauer and citrate (IMViC) test was performed to determine the complete microbiological profile of the water samples. Almost all the water samples were contaminated with Total Coliforms (TC). A high concentration of TC (>103 CFU/mL) was found in 8 samples (36.4

关键词： Water Assessment Micro Biological Survey Method Biochemical IMViC Test West Cameroon

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Factors associated with DAA virological treatment failure and resistance-associated substitutions description in HIV/HCV coinfected patients

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World Journal of Hepatology 2018年第11期10卷 856-866页

作者： Dominique Salmon Pascale Trimoulet Camille Gilbert Caroline Solas Eva Lafourcade Julie Chas Lionel Piroth Karine Lacombe Christine Katlama Gilles Peytavin Hugues Aumaitre laurent Alric Franoois Boué Philippe Morlat Isabelle Poizot-martin Eric Billaud Eric Rosenthal Alissa Naqvi Patrick Miailhes Firouzé Bani-Sadr laure Esterle Patrizia Carrieri Franoois Dabis Philippe Sogni Linda Wittkop 无 Assistance Publique des Hopitaux de Paris Hopitaux Universitaires Paris CentreHopital Hotel DieuUnitédes Maladies infectieuses et tropicalesParis 75004France Université Paris Descartes Sorbonne Paris CitéParis 75006France Trimoulet CHU de BordeauxHopital PellegrinLaboratoire de VirologieBordeaux 33000France Trimoulet CNRS-UMR 5234Microbiologie fondamentale et PathogénicitéUniversitéde BordeauxBordeaux 3000France Philippe Morlat Laure EsterleFrancois DabisLinda WittkopUniv.BordeauxISPEDInsermBordeaux Population Health Research Centerteam MORPH3EUSUMR 1219CIC-EC 1401Bordeaux F-33000France APHM Hopital La TimoneLaboratoire de Pharmacocinétique et ToxicologieMarseille 13005France Centre Hospitalier Universitaire de Dijon Département d'InfectiologieDijon cedex 21079France INSERM-CIC 1342 Universitéde Bourgogne Dijon 21000France Assistance Publique des Hopitaux de Paris GHUEP site Saint-AntoineServices Maladies infectieuses et tropicalesParis 75011France UniversitéPierre et Marie Curie UMR S1136Institut Pierre Louis d' Epidémiologie et de SantéPubliqueParis 75646France UniversitéParis-Sorbonne Paris 75005France Assistance Publique des Hopitaux de Paris Hopital PitiéSalpêtrière Services Maladies infectieuses et tropicalesParis 75013France Assistance Publique des Hopitaux de Paris Hopital Bichat-Claude BernardLaboratoire de PharmacologieParis 75877France IAME UMR 1137Sorbonne Paris CitéINSERMUniversitéParis DiderotParis 75890France Centre Hospitalier de Perpignan Service Maladies infectieuses et tropicalesPerpignan 66000Franc Centre Hospitalier Universitaire de Toulouse Hopital PurpanService Médecine interne-Pole DigestifToulouse 31300France UMR 152 IRD UniversitéToulouseⅢ Paul SabatierToulouse 31330France Hopital Antoine-Béclère Assistance Publique des Hopitaux de ParisUniversitéParis SudService Médecine interne et immunologieClamart 92140France Centre Hospitalier Universitaire de Bordeaux Service de médecine interneHopital Saint-AndréBordeaux 33000France Uni

AIMTo describe factors associated with treatment failure and frequency of resistance-associated substitutions （RAS）.METHODSHuman immunodefciency virus （HIV）/hepatitis C virus （HCV） coinfected patients starting a first direct-acting antiviral （DAA） regimen before February 2016 and included in the French ANRS CO13 HEPAVIH cohort were eligible. Failure was defned as：（1） non-response [HCV-RNA remained detectable during treatment, at end of treatment （EOT）]; and （2） relapse （HCV-RNA suppressed at EOT but detectable thereafter）. Sequencing analysis was performed to describe prevalence of drug class-specifc RAS. Factors associated with failure were determined using logistic regression *** 559 patients, 77% had suppressed plasmaHIV-RNA 〈 50 copies/mL at DAA treatment initiation41% were cirrhotic, and 68% were HCV treatmentexperienced. Virological treatment failures occurred in22 patients and were mainly relapses （17, 77%） thenundefined failures （3, 14%） and non-responses （29%）. Mean treatment duration was 16 wk overall. Posttreatment NS3, NS5A or NS5B RAS were detected in10/14 patients with samples available for sequencinganalysis. After adjustment for age, sex, ribavirin useHCV genotype and treatment duration, low platelecount was the only factor signifcantly associated with ahigher risk of failure （OR： 6.5; 95%CI： 1.8-22.6）. CONCLUSIONOnly 3.9% HIV-HCV coinfected patients failed DAAregimens and RAS were found in 70% of those failingLow platelet count was independently associated withvirological failure.

关键词： Human immunodeficiency virus Hepatitis C virus Direct-acting antiviral Treatment virological failure Resistant associated mutations

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Global prevalence of occult hepatitis C virus: A systematic review and meta-analysis

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World Journal of Methodology 2022年第3期12卷 179-190页

作者： Donatien Serge Mbaga Sebastien Kenmoe Jacky Njiki Bikoï Guy Roussel Takuissu Marie Amougou-Atsama Etienne Atenguena Okobalemba Jean Thierry Ebogo-Belobo Arnol Bowo-Ngandji martin Gael Oyono Jeannette Nina Magoudjou-Pekam Ginette Irma Kame-Ngasse Alex Durand Nka Alfloditte Flore Feudjio Cromwel Zemnou-Tepap Elie Adamou Velhima Juliette laure Ndzie Ondigui Rachel Audrey Nayang-Mundo Sabine Aimee Touangnou-Chamda Yrene Kamtchueng Takeu Jean Bosco Taya-Fokou Chris Andre Mbongue Mikangue Raoul Kenfack-Momo Cyprien Kengne-Ndé Carole Stephanie Sake Seraphine Nkie Esemu Richard Njouom Lucy Ndip Sara Honorine Riwom Essama Department of Microbiology The University of Yaounde IYaounde 00237Cameroon Department of Microbiology and Parasitology University of BueaBuea 00237Cameroon Department of Virology Centre Pasteur of CameroonYaounde 00237Cameroon Centre of Research in Food Food Security and NutritionInstitute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Centre de Recherche sur les Maladies Emergentes et Re-Emergentes Institute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Faculty of Medicine and Biomedical Science The University of Yaounde IYaounde 00237Cameroon Medical Research Centre Institute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Laboratory of Parasitology and Ecology The University of Yaounde IYaounde 00237Cameroon Department of Biochemistry The University of Yaounde IYaounde 00237Cameroon Virology Laboratory Chantal Biya International Reference Center for Research on HIV/AIDS Prevention and ManagementYaounde 00237Cameroon

BACKGROUND Occult hepatitis C infection(OCI)is characterized by the presence of hepatitis C virus(HCV)RNA in the liver,peripheral blood mononuclear cells(PBMC)and/or ultracentrifuged serum in the absence of detectable HCV-RNA in *** has been described in several categories of populations including hemodialysis patients,patients with a sustained virological response,immunocompromised individuals,patients with abnormal hepatic function,and apparently healthy *** To highlight the global prevalence of *** We performed a systematic and comprehensive literature search in the following 4 electronic databases PubMed,EMBASE,Global Index Medicus,and Web of Science up to 6th May 2021 to retrieve relevant studies published in the *** studies were unrestricted population categories with known RNA status in serum,PBMC,liver tissue and/or ultracentrifuged *** were extracted independently by each author and the Hoy et al tool was used to assess the quality of the included *** used the random-effect meta-analysis model to estimate the proportions of OCI and their 95%confidence intervals(95%CI).The Cochran's Q-test and the I2 test statistics were used to assess heterogeneity between *** plot and Egger test were used to examine publication bias.R software version 4.1.0 was used for all *** The electronic search resulted in 3950 *** obtained 102 prevalence data from 85 included *** pooled prevalence of seronegative OCI was estimated to be 9.61%(95%CI:6.84-12.73)with substantial heterogeneity[I^(2)=94.7%(95%CI:93.8%-95.4%),P<0.0001].Seropositive OCI prevalence was estimated to be 13.39%(95%CI:7.85-19.99)with substantial heterogeneity[I^(2)=93.0%(90.8%-94.7%)].Higher seronegative OCI prevalence was found in Southern Europe and Northern Africa,and in patients with abnormal liver function,hematological disorders,and kidney *** seropositive OCI prevalence was found in Southern Europe,Northern

关键词： Occult hepatitis C virus infection Prevalence Worldwide Peripheral blood mononuclear cells Hepatitis C virus

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