检索结果-南通市图书馆

Diversification of SUMO-Activating Enzyme in Arabidopsis： Implications in SUMO Conjugation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2013年第5期6卷 1646-1660页

作者： laura Castano-Miquel Josep Segui Silvia Manrique ines Teixeira Lorenzo Carretero-Paulet Felix Atencio L. Maria Lois Center for Research in Agricultural Genomics CRAG (CSIC-IRTA-UAB-UB) Edifici CRAG-Campus UAB Bellaterra (Cerdanyola del Valles) 08193 Barcelona Spain Department of Biological Sciences SUNY-University at Buffalo North Campus 109 Cooke Hall Buffalo NY 14260 USA

Sumoylation is an essential posttranslational modification that participates in many biological processes including stress responses. However, little is known about the mechanisms that control Small Ubiquitin-like MOdifier （SUMO） conjugation in vivo. We have evaluated the regulatory role of the heterodimeric E1 activating enzyme, which catalyzes the first step in SUMO conjugation. We have established that the E1 large SAE2 and small SAE1 subunits are encoded by one and three genes, respectively, in the Arabidopsis genome. The three paralogs genes SAEla, SAElbl, and SAElb2 are the result of two independent duplication events. Since SAElbl and SAElb2 correspond to two identical cop- ies, only two E1 small subunit isoforms are present in vivo： SAEla and SAElb. The E1 heterodimer nuclear localization is modulated by the C-terminal tail of the SAE2 subunit. In vitro, SUMO conjugation rate is dependent on the SAE1 isoform contained in the E1 holoenzyme and our results suggest that downstream steps to SUMO-E1 thioester bond formation are affected. In vivo, SAEla isoform deletion in T-DNA insertion mutant plants conferred sumoylation defects upon abi- otic stress, consistent with a sumoylation defective phenotype. Our results support previous data pointing to a regula- tory role of the E1 activating enzyme during SUMO conjugation and provide a novel mechanism to control sumoylation in vivo by diversification of the E1 small subunit.

关键词： E1 activating enzyme conjugation rate subcellular localization regulation abiotic stress gene duplication.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

夸特罗科蒂商务中心俄罗斯圣彼德堡

世界建筑导报 2018年第5期33卷 97-99页

作者： yusuke aizawa momoko asano ilan balouka filippo carcano erica cazzaniga cristina castelli maiko chiriki giuseppe crocco ola abetskaya alessandro laner alessandro mauri luca mazzeo elena migliorati hirotaka oishi an-drea palaia miguel pallares nicola pelucchi elena petko laura ines quatela daniele reimondo daniela scarpa marta sesana roberto songini olga strikha sarah trianni enrico venturini andrea martiradonna(摄)

夸特罗科蒂（Quattro Corti）商务中,15落成于俄罗斯古城圣彼德堡，紧邻圣以撒大教堂。该项目是在保留原有两座大楼立面的基础上，构造新的现代化建筑。设计以新型金属结构屋顶重新连接了原有建筑斜坡式的楼顶。屋顶在选材和形状上与城... 详细信息

夸特罗科蒂（Quattro Corti）商务中,15落成于俄罗斯古城圣彼德堡，紧邻圣以撒大教堂。该项目是在保留原有两座大楼立面的基础上，构造新的现代化建筑。设计以新型金属结构屋顶重新连接了原有建筑斜坡式的楼顶。屋顶在选材和形状上与城市天际线完美相融。屋顶之下是四个庭院，既保证了内部空间享有充足光源，又可以用于举行艺术造型、展览等公共活动。庭院立面覆盖以角度不同的反光玻璃板。

关键词：商务中心俄罗斯现代化建筑城市天际线金属结构内部空间艺术造型公共活动

SUMOylation Inhibition Mediated by Disruption of SUMO El-E2 Interactions Confers Plant Susceptibility to Necrotrophic Fungal Pathogens

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第5期10卷 709-720页

作者： laura Castano-Miquel Abraham Mas ines Teixeira Josep Segui Anna Perearnau Bhagyasree N. Thampi Arnaldo L. Schapire Natalia Rodrigo Gaelle La Verde Silvia Manrique Maria Coca L. Maria Lois Center for Research in Agricultural Genomics - CRAG Edifici CRAG-Campus UAB Bellaterra (Cerdanyola del Vall~s) 08193 Barcelona Spain

Protein modification by SUMO modulates essential biological processes in eukaryotes. SUMOylation is facilitated by sequential action of the El-activating, E2-conjugating, and E3-1igase enzymes. In plants, SUMO regulates plant development and stress responses, which are key determinants in agricultural productivity. To generate additional tools for advancing our knowledge about the SUMO biology, we have developed a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO El-E2 interactions through expression of E1 SAE2uFDct domain. Targeted mutagenesis and phylogenetic analyses revealed that this inhibition involves a short motif in SAE2uFDct highly divergent across kingdoms. Transgenic plants expressing the SAE2UFDCt domain displayed dose-dependent inhibition of SUMO conjugation, and have revealed the existence of a post-transcriptional mechanism that regulates SUMO E2 conjugating enzyme levels. Interestingly, these transgenic plants displayed increased suscep- tibility to necrotrophic fungal infections by Botrytis cinerea and Plectosphaerella cucumerina. Early after fungal inoculation, host SUMO conjugation was post-transcriptionally downregulated, suggesting that targeting SUMOylation machinery could constitute a novel mechanism for fungal pathogenicity. These findings support the role of SUMOylation as a mechanism involved in plant protection from environmental stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be applicable in important crop plants and other non-plant organisms regardless of their genetic complexity.

关键词： SUMQ inhibition development flowering necrotrophic fungi El-activating enzyme El-E2interaction disruption

Water Quality, Contamination, and Wetlands in the Croton Watershed, New York, USA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Modern Hydrology 2012年第1期2卷 7-14页

作者： Jeffrey M. McKenzie Donald I. Siegel laura K. Lautz Martin H. Otz James Hassett ines Otz 不详

The Croton Watershed (New York State, USA) is a semi-urban region that provides 10% of the drinking water for the City of New York. Nonpoint source contamination in the watershed is a major concern for managers because the water supply is currently unfiltered water. Results are reported from three synoptic studies of surface water quality from 98 wetland-containing sub-catchments in the Croton Watershed designed to broadly characterize, at a reconnaissance level, the geochemical controls on water quality, in particular as it relates to wetlands. Total dissolved organic carbon concentrations in surface waters draining wetlands correlated well (average R2 of 0.93) with standard Gelbstoff (g440) color measurements, although there is very little correlation between dissolved organic carbon concentrations and wetland areas in the sub-catchments. This may be a potential indication of other sources of colored organic material. Concentrations of dissolved sodium and chloride, while related to road length, stochiometrically had more chloride than expected for pure road-salt dissolution. This offset is likely due to cation exchange and sorbtion of sodium by wetlands in the Croton watershed. The results show contamination in the Croton hydrologic system that should addressed in ongoing management policies and decision-making.

关键词： Wetlands Croton Watershed Geochemistry Water Quality

CTNS Molecular Genetics Profile in a Portuguese Cystinosis Population

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Genetics 2018年第4期8卷 91-100页

作者： Filipa Ferreira ines Leal David Sousa Teresa Costa Conceicao Mota Ana Marta Gomes Daniela Lopes Maria do Carmo Macario Isabel Tavares Helena Pinto Joao Paulo Oliveira Rita Magrico Celia Carmona Sonia Ramos Raquel Neiva Ana Marcao laura Vilarinho Newborn Screening Metabolism and Genetics UnitHuman Genetics DepartmentNational Institute of Health Dr Ricardo JorgePortoPortugal Centro Hospitalar Lisboa Norte Hospital de Santa MariaLisboaPortugal Centro Hospitalar Universitario do Porto PortoPortugal Centro Hospitalar Vila Nova de Gaia EspinhoPortugal Centro Hospitalar Universitario de Coimbra CHUCCoimbraPortugal Servico de Genetica Medica Centro Hospitalar de Sao JoaoPortoPortugal Unidade de Genetica Faculdade de Medicina da Universidade do PortoPortoPortugal I3S—Instituto de Investigacao e Inovacao em Saude PortoPortugal Servico de Nefrologia do Hospital Garcia de Orta AlmadaPortugal Research and Development Unit Department of Human GeneticsNational Institute of Health Dr Ricardo JorgePortoPortugal

Background: Cystinosis is a multisystemic autosomal recessive deficiency of the lysosomal membrane transporter protein (cystinosin) caused by mutations in CTNS gene. Objective: This study summarizes the Portuguese experience in the diagnosis and management of patients with this rare disease over the past few years and reports recurrent mutations in the CTNS gene. Methods: Unrelated patients from different pediatric and adult hospitals all over Portugal with non-nephrotic proteinuria, hypercalciuria, hypokalemia impaired proximal reabsorption of amino acids, glycosuria and hypophosphatemia, suggestive of a Fanconi syndrome and ocular problems, were studied. Intra-leukocyte cystine levels were determined and molecular analysis was performed, to determine the presence or absence of the 57-kb deletion in CTNS, followed by direct sequencing of the coding exons of CTNS. Results: From 1998 to 2017, twenty-one cystinotic patients were biochemically diagnosed. From the remaining seventeen (four deceased), eleven were studied for CTNS gene. Five out of eleven patients were homozygous for the 57-kb deletion (10/22;45.5%), and other five were compound heterozygous for this variant (15/22;68.2%). The other mutations found were p.Q128X (c.721 C>T;2/22), p.S139F (c.755 C>T;4/22) and c.18-21delGACT (p.T7FfsX7;1/22). All of these seventeen cystinotic patients are in treatment. Approximately 84% are adults, 16% are young children, and 54.5% are kidney transplant recipient. Conclusions: The authors would like to emphasize the importance of first screening for the 57-kb deletion since it is very common in our population. This genetic study is the first in our country and it could be the basis for future genetic counseling in Portuguese population.

关键词： Cystinosis CTNS Gene Mutational Spectrum Kidney Failure 57-kb Deletion

Inter-Laboratory Ring Trial to Evaluate Reverse Transcription Polymerase Chain Reaction Methods Used for <i>Dolphin Morbillivirus</i>Detection in Italy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2019年第5期9卷 423-437页

作者： Zoccola Roberto Pautasso Alessandra Grattarola Carla Monnier Milena Miceli Ilaria Ingravalle Francesco Crescio Maria ines Giorda Federica Masoero Loretta Zaccaria Guendalina Purpari Giuseppa Cersini Antonella Viscardi Maurizio Serracca laura Padalino Iolanda Puggioni Giantonella Canonico Cristina Toffan Anna Centelleghe Cinzia Di Francesco Cristina Esmeralda Mazzariol Sandro Di Guardo Giovanni Mignone Walter Casalone Cristina Goria Maria Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta Torino Italy Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise Teramo Italy Istituto Zooprofilattico Sperimentale della Sicilia Palermo Italy Istituto Zooprofilattico Sperimentale del Lazio e della Toscana Roma Italy Istituto Zooprofilattico Sperimentale del Mezzogiorno Portici (NA) Italy Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta La Spezia Italy Istituto Zooprofilattico Sperimentale della Puglia e Basilicata Foggia Italy Istituto Zooprofilattico Sperimentale della Sardegna Sassari Italy Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche Ancona Italy Istituto Zooprofilattico Sperimentale delle Venezie Legnaro (PD) Italy University of Padova Department of Comparative Biomedicine and Food Science (BCA) Legnaro (PD) Italy Faculty of Veterinary Medicine University of Teramo Teramo Italy Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta Imperia Liguria Italy

Dolphin Morbillivirus (DMV) is one of the most frequently detected pathogens in stranded cetacean specimens worldwide as well as in Italy. Due to the persistence of DMV in the Mediterranean Sea and to the lack of information about the efficiency of the available diagnostic techniques, the Italian National Reference Centre for diagnostic activities on dead stranded marine mammals (C.Re.Di.Ma) performed the first inter-laboratory ring trial with the aim to standardize a diagnostic biomolecular approach for DMV in Italy. Viral isolation is usually considered the “gold standard” for the definitive diagnosis of most pathogens, but it is not often feasible in DMV diagnosis, due to the poor preservation of virus-targeted tissues in stranded cetacean carcasses, as well as to the lack of appropriate sensitivity of cell lines towards DMV variability. Therefore direct viral detection on tissues by means of reverse transcription-PCR (RT-PCR) represents a valuable option for DMV infection’s diagnosis. For detecting DMV in cetacean die-offs occurred in the Mediterranean basin since 2013, C.Re.Di.Ma developed an RT-PCR based method targeting to a 287 bp fragment of DMV nucleoprotein (N) gene. With the purpose to evaluate its performances in terms of accuracy (Se = sensitivity and Sp = specificity) and precision (reproducibility), it was submitted to a ring trial. So, 12 Public Laboratories belonging to the Italian dead stranded marine mammals diagnostic network were asked to analyze a panel of 40 samples (positive and negative for DMV, using different dilutions of a viral suspension obtained from a cell culture supernatant of a DMV strain) with the aforementioned technique. Furthermore, we also aimed at comparing the accuracy of other 7 molecular methods routinely applied for DMV detection in Italy. For this purpose, the second panel of identical 40 DMV +ve and -ve samples was provided to Laboratories that routinely used DMV detection methods other than those developed by C.Re.Di.Ma, in order to be analyzed simultaneously with

关键词： Dolphin Morbillivirus RT-PCR Interlab Ring Test Method Comparison Stranded Cetaceans Mediterranean Sea