检索结果-南通市图书馆

Addition of heparin binding sites strongly increases the bone forming capabilities of BMP9 in vivo

Bioactive Materials 2023年第11期29卷 241-250页

作者： Claudia Siverino Shorouk Fahmy-Garcia Viktoria Niklaus Nicole Kops laura dolcini Massimiliano Maraglino Misciagna Yanto Ridwan Eric Farrell Gerjo JVMvan Osch Joachim Nickel Department of Tissue Engineering and Regenerative Medicine University Hospital WuerzburgWuerzburgGermany Department of Orthopaedics and Sports Medicine Erasmus MCUniversity Medical CenterRotterdamthe Netherlands Department of Internal Medicine Erasmus MCUniversity Medical CenterRotterdamthe Netherlands Department of Oral and Maxillofacial Surgery Erasmus MCUniversity Medical CenterRotterdamthe Netherlands Fin-Ceramica Faenza SpA Via Granarolo 177/348018FaenzaItaly AMIE Core Facility Erasmus University Medical CenterRotterdamthe Netherlands Department of Otorhinolaryngology Head and Neck SurgeryErasmus MCUniversity Medical CenterRotterdamthe Netherlands Department of Biomechanical Engineering Faculty of MechanicalMaritime and Materials EngineeringDelft University of TechnologyDelftthe Netherlands Fraunhofer ISC Translational Center RTWuerzburgGermany

Bone Morphogenetic proteins(BMPs)like BMP2 and BMP7 have shown great potential in the treatment of severe bone *** recent in vitro studies,BMP9 revealed the highest osteogenic potential compared to other BMPs,possibly due to its unique signaling pathways that differs from other osteogenic ***,in vivo the bone forming capacity of BMP9-adsorbed scaffolds is not superior to BMP2 or *** silico analysis of the BMP9 protein sequence revealed that BMP9,in contrast to other osteogenic BMPs such as BMP2,completely lacks so-called heparin binding motifs that enable extracellular matrix(ECM)interactions which in general might be essential for the BMPs’osteogenic ***,we genetically engineered a new BMP9 variant by adding BMP2-derived heparin binding motifs to the N-terminal segment of BMP9′s mature *** resulting protein(BMP9 HB)showed higher heparin binding affinity than BMP2,similar osteogenic activity in vitro and comparable binding affinities to BMPR-II and ALK1 compared to ***,remarkable differences were observed when BMP9 HB was adsorbed to collagen scaffolds and implanted subcutaneously in the dorsum of rats,showing a consistent and significant increase in bone volume and density compared to BMP2 and *** at 10-fold lower BMP9 HB doses bone tissue formation was *** innovative approach of significantly enhancing the osteogenic properties of BMP9 simply by addition of ECM binding motifs,could constitute a valuable replacement to the commonly used *** possibility to use lower protein doses demonstrates BMP9 HB’s high translational potential.

关键词： Bone morphogenetic protein 9(BMP9) Heparin binding sites Bone regeneration Subcutaneous animal model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：