检索结果-南通市图书馆

Infertility treatment, a matter of a lovely sperm？

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2013年第6期15卷 719-720页

作者： laura C Giojalas Centro de Biologfa Celular y Molecular & Instituto deInvestigaciones Biol6gicas y tecnol6gicas (CONICEt-Universidad Nacional de Cordoba) Av. Velez Sasrfield1611 X5016GCA - Cordoba Argentina

Nowadays, the efficiency of the infertil-ity treatment is relatively low. One of the cues to counteract this problem relies on the optimum selection of spermatozoa. We developed a new method （sperm selection assay （SSA）） based on the chemical attrac-tion of spermatozoa that are at the best functional state. Additionally, the SSA leads spermatozoa to complete and/or acquire the competence to fertilize the egg. these effects are equally observed either in nor-mal or subfertile semen samples. those cap-abilities of SSA may improve the success of current infertility treatment.

关键词： GUIDANCE

Using lysosomal membrane stability of haemocytes in Ruditapes philippinarum as a biomarker of cellular stress to assess contamination by caffeine, ibuprofen, carbamazepine and novobiocin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2013年第7期25卷 1408-1418页

作者： Gabriela V. Aguirre-Martínez Sara Buratti Elena Fabbri Angel t. DelValls M. laura Martín-Díaz Unitwin/Unesco/WiCoP Physical Chemistry Department University of Cdiz Faculty of Marine and Environmental SciencesPolígono Río San Pedro s/n 11510 Puerto Real Cdiz Spain Andalusian Center for Marine Science and technology (CACYtMAR) Campus Universitariode Puerto Real11510 Puerto Real Cdiz Spain University of Bologna Interdepartment Centre for Research in Environmental Sciences (CIRSA)via S. Alberto 16348123 Ravenna Italy

Although pharmaceuticals have beendetected in the environment only in the range from ng/L to μg/L, it has beendemonstrated that they can adversely affect the health status of aquatic organisms. Lysosomal membrane stability （LMS） has previously been applied as an indicator of cellularwell-being todetermine health status in bivalve mussels. the objective of this study is to evaluate LMS in Ruditapes philippinarum haemolymph using the neutral red retention assay （NRRA）. Clamswere exposed in laboratory conditions to caffeine （0.1, 5, 15, 50 μg/L）, ibuprofen （0.1, 5, 10, 50 μg/L）, carbamazepine and novobiocin （both at 0.1, 1, 10, 50 μg/L） for35days. Results show adose-dependent effect of the pharmaceuticals. the neutral red retention time measured at the end of the bioassaywas significantly reduced by 50% after exposure to environmental concentrations （p 〈 0.05）（caffeine = 15 μg/L; ibuprofen = 10 μg/L; carbamazepine = 1 μg/L and novobiocin = 1 μg/L）, compared to controls. Clams exposed to these pharmaceuticalswere considered to present adiminished health status （retention time 〈45 min）, significantlyworse than controls （96 min）（p 〈 0.05）. the predicted no environmental effect concentration （PNEC） results showed that these pharmaceuticals are very toxic at the environmental concentrations tested. Measurement of the alteration of LMS has been found to be a sensitive technique that enables evaluation of the health status of clams after exposure to pharmaceuticals under laboratory conditions, thus representing a robust tier-1 screening biomarker.

关键词： neutral red pharmaceuticals bioassay haemolymph bivalves health status Manila clam

Applying Gregory Johnson’s Concepts of Scalar Stress to Scale and Information thresholds in Holocene Social Evolution

在线全文

学校读者我要写书评

暂无评论

Journal of Social Computing 2022年第1期3卷 38-56页

作者： laura J.Ellyson the Department of Anthropology Washington State UniversityPullmanWA 99163USA terrestrial Archaeology Division SEARCH Inc.PensacolaFL 32503USA.

Although Gregory Johnson’s models have influenced social theory in archaeology,few have applied or built upon these models to predict aspects of social organization,group size,or *** have been limited to small case ***,the relationship between a society’s scale and its information-processing capacities has been explored using the Seshat ***,I apply multiple-linear regression analysis to the Seshat data using turchin and colleagues’9“complexity characteristics”(CCs)to further examine the relationship between the hierarchy CC and the remaining 8 CCs which include both aspects of a polity’s scale and aspects of what Kohler et ***“collective computation”.the results support Johnson’s ideas that stratification will generally increase with increases in a polity’s scale(population,territory);however,stratification is also higher when polities increase their developments in information-processing variables such as texts.

关键词： hierarchy scalar stress Seshat Databank multiple imputation multiple regression

Structural and functional aspects of the Helicobacter pylori secretome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第6期20卷 1402-1423页

作者： Giuseppe Zanotti laura Cendron Department of Biomedical Sciences University of Padua Department of Biology University of Padua

Proteins secreted by Helicobacter pylori (H. pylori), an important human pathogen responsible for severe gastric diseases, are reviewed from the point of view of their biochemical characterization, both functional and structural. Despite the vast amount of experimental data available on the proteins secreted by this bacterium, the precise size of the secretome remains unknown. In this review, we consider as secreted both proteins that contain a secretion signal for the periplasm and proteins that have been detected in the external medium in in vitro experiments. In this way, H. pylori’s secretome appears to be composed of slightly more than 160 proteins, but this number must be considered very cautiously, not only because the definition of secretome itself is ambiguous but also because the included proteins were observed as secreted in in vitro experiments that were not representative of the environmental situation in vivo. the proteins that appear to be secreted can be grouped into different classes: enzymes (48 proteins), outer membrane proteins (43), components of flagella (11), members of the cytotoxic-associated genes pathogenicity island or other toxins (8 and 5, respectively), binding and transport proteins (9), and others (11). A final group, which includes 28 members, is represented by hypothetical uncharacterized proteins. Despite the large amount of data accumulated on the H. pylori secretome, a considerable amount of work remains to reach a full comprehension of the system at the molecular level.

关键词： Helicobacter pylori Secreted proteins Periplasmic space Secretion signal Secretome

Sensitivity analysis of sediment flux derived by laser diffraction and acoustic backscatter within a reservoir

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Sediment Research 2018年第1期33卷 18-26页

作者： Stefan Haun laura Lizano Institute for Modelling Hydraulic and Environmental Systems University of Stuttgart Department of Hydraulic and Environmental Engineering Norwegian University of Science and Technology Ministry of Environment and Energy

A combination of two indirect methods to measure sediment flux is presented in this study to evaluate suspended sediment transport in a hydropower reservoir. the acoustic backscatter signal(ABS)from an Acoustic Doppler Current Profiler(ADCP) is therefore applied in pre-defined transects within the reservoir in combination with a Laser In-Situ Scattering transmissometry-stream lined device(LISStSL). the stationary LISSt-SL derived suspended sediment concentration(SSC) measurements are used to calibrate the ABS. From the LISSt-SL measurements a time series of SSC is obtained. this enables, in addition, a comprehensive data analysis to evaluate the influence of natural fluctuations of the SSC on the calculated sediment flux, which should be taken into account when assessing sediment transport. Furthermore SSC measurements are done with the LISSt-SL close to the reservoir bed. In areas close to the bed no information regarding the ABS is available from the ADCP measurements due to the side-lobe interference. In various studies the information from the last three valid cells is used for ***, as result of a comparison of the LISSt-SL measurements with extrapolated SSC values from the ADCP measurements it can be seen that, especially in deep reservoirs, this method has to be adapted to the in-situ conditions.

关键词： Reservoir Suspended sediment concentrations Sediment flux Laser diffraction Acoustic backscatter signal

Implications of metabolism-driven myeloid dysfunctions in cancer therapy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2021年第4期18卷 829-841页

作者： laura Strauss Valentina Guarneri Alessandra Gennari Antonio Sica Department of Immunopharmacology and Immuno Oncology EMD Serono Research&Development InstituteInc.80119545A Middlesex TurnpikeBillericaMA 01821USA Department of Surgery Oncology and GastroenterologyUniversity of PadovaPadovaItaly Veneto Institute of Oncology-IRCCS PaduaItalyPadovaItaly Division of Oncology Department of Translational MedicineUniversity of Eastern PiedmontNovaraItaly Department of Pharmaceutical Sciences University of Eastern PiedmontA.Avogadrovia Bovio 6NovaraItaly Humanitas Clinical and Research Center IRCCSVia Manzoni 56RozzanoMilanItaly

Immune homeostasis is maintained by an adequate balance of myeloid and lymphoid *** chronic inflammatory states,including cancer,this balance is lost due to dramatic expansion of myeloid progenitors that fail to mature to functional inflammatory neutrophils,macrophages,and dendritic cells(DCs),thus giving rise to a decline in the antitumor effector lymphoid ***-related inflammation orchestrates the production of hematopoietic growth factors and cytokines that perpetuate recruitment and activation of myeloid precursors,resulting in unresolved and chronic *** pathologic inflammation creates profound alterations in the intrinsic cellular metabolism of the myeloid progenitor pool,which is amplified by competition for essential nutrients and by hypoxia-induced metabolic rewiring at the tumor ***,persistent myelopoiesis and metabolic dysfunctions contribute to the development of cancer,as well as to the severity of a broad range of diseases,including metabolic syndrome and autoimmune and infectious *** aims of this review are to(1)define the metabolic networks implicated in aberrant myelopoiesis observed in cancer patients,(2)discuss the mechanisms underlying these clinical manifestations and the impact of metabolic perturbations on clinical outcomes,and(3)explore new biomarkers and therapeutic strategies to restore immunometabolism and differentiation of myeloid cells towards an effector phenotype to increase host antitumor *** propose that the profound metabolic alterations and associated transcriptional changes triggered by chronic and overactivated immune responses in myeloid cells represent critical factors influencing the balance between therapeutic efficacy and immune-related adverse effects(irAEs)for current therapeutic strategies,including immune checkpoint inhibitor(ICI)therapy.

关键词： Myelopoiesis tumor-associated macrophages Myeloid-derived suppressor cells Metabolism Cancer therapy

Longitudinal bending stiffness does not affect running economy in Nike Vaporfly Shoes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2022年第3期11卷 285-292页

作者： laura A.Healey Wouter Hoogkamer Integrative Locomotion Laboratory Department of KinesiologyUniversity of MassachusettsAmherstMA 01003-9258USA

Purpose this study aimed to determine the independent effect of the curved carbon-fiber plate in the Nike Vaporfly 4%shoe on running economy and running *** Fifteen healthy male runners completed a metabolic protocol and a biomechanics *** both protocols participants wore 2 different shoes,an intact Nike Vaporfly 4%(VF_(intact))and a cut Nike Vaporfly 4%(VF_(cut)).the VF_(cut) had 6 medio-lateral cuts through the carbon-fiber plate in the forefoot to reduce the effectiveness of the *** the metabolic protocol,participants ran at 14 km/h for 5 min,twice with each shoe,on a force-measuring treadmill while we measured metabolic *** the biomechanics protocol,participants ran across a runway with embedded force plates at 14 km/*** calculated running economy,kinetics,and lower limb joint *** Running economy did not significantly differ between shoe conditions(on average,0.55%±1.77%(mean±SD))worse in the VF_(cut) compared to the VF_(intact);95%confidence interval(-1.44%to 0.40%).Biomechanical differences were only found in the metatarsophalangeal(MtP)joint with increased MtP dorsiflexion angle,angular velocity,and negative power in the VF_(cut).Contact time was 1%longer in the VF_(intact).Conclusion Cutting the carbon-fiber plate and reducing the longitudinal bending stiffness did not have a significant effect on the energy savings in the Nike Vaporfly 4%.this suggests that the plate's stiffening effect on the MtP joint plays a limited role in the reported energy savings,and instead savings are likely from a combination and interaction of the foam,geometry,and plate.

关键词： Biomechanics Footwear Mechanical doping Metabolic rate Performance

Effects of Different Electromagnetic Fields on Circadian Rhythms of Some Haematochemical Parameters in Rats

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2009年第4期22卷 348-353页

作者： laura CONtALBRIGO CALOGERO StELLEttA laura FALCIONI StEFANIA CASELLA GIUSEPPE PICCIONE MORANDO SOFFRIttI AND MASSIMO MORGANtE European Foundation of Oncology and Environmental Sciences "B. Ramazzini" Department of Clinical Veterinary Science University of Padova Department of Experimental Sciences and Applied Biotechnology Faculty of Veterinary Medicine University of Messina

Objective to investigate the effects of different electromagnetic fields on some haematochemical parameters of circadian rhythms in Sprague-Dawley rats. Methods the study was carried out in 18 male and 18 female rats in good health conditions exposed to 50 Hz magnetic sinusoid fields at the intensity of 1000 μt, 100 μt, and 0 μt （control group） respectively, and in 18 male and 18 female rats in good health conditions exposed to 1.8 GHz electromagnetic fields at the intensity of 50 V/m, 25 V/m and 0 V/m （control group）, respectively. Following haematochemical parameters for glucose, triglycerides, and total cholesterol were measured. Results Different effects of electromagnetic fields on circadian rhythms of both male and female rats were observed. Different changes occurred in some haematochemical parameters for glucose, triglycerides, and total cholesterol （P〈0.05）. Conclusion Exposure to different electromagnetic fields is responsible for the variations of some haematochemical parameters in rats.

关键词： Extremely low frequency electromagnetic fields (50 Hz) Radiofrequency electromagnetic fields (1.8 GHz) Haematochemical parameters Circadian rhythm Sprague-Dawley rat

therapeutic potential of α7 nicotinic receptor agonists to regulate neuroinflammation in neurodegenerative diseases

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第9期12卷 1418-1421页

作者： laura Foucault-Fruchard Daniel Antier UMR INSERM U930 Université Fran?ois Rabelais CHRU de tours H?pital Bretonneau

Neurodegenerative diseases, such as Alzheimer＇s, Parkinson＇s and Huntington＇s diseases, are all character- ized by a component of innate immunity called neuroinflammation. Neuronal loss and neuroinflammation are two phenomena closely linked. Hence, the neuroinflammation is a relevant target for the management of the neurodegenerative diseases given that, to date, there is no treatment to stop neuronal loss. Several studies have investigated the potential effects of activators of alpha 7 nicotinic acetylcholine receptors in animal models of neurodegenerative diseases. these receptors are widely distributed in the central nervous system. After activation, they seem to mediate the cholinergic anti-inflammatory pathway in the brain. this anti-inflammatory pathway, first described in periphery, regulates activation of microglial cells considered as the resident macrophage population of the central nervous system. In this article, we shortly review the agonists of the alpha 7 nicotinic acetylcholine receptors that have been evaluated in vivo and we focused on the selective positive allosteric modulators of these receptors. these compounds represent a key element to enhance receptor activity only in the presence of the endogenous agonist.

关键词： α7 nicotinic receptors cholinergic anti-inflammatory pathway Alzheimer's disease Huntington's disease Parkinson's disease neuroinflammation neurodegeneration positive allosteric modulators