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Effects of extender and packaging method on morphological and functional characteristics of cryopreserved Ossimi ram semen

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Asian pacific Journal of Reproduction 2020年第3期9卷 148-155页

作者： Wael A.Khalil Abdel-Khalek E.Abdel-Khalek laura falchi Bedir E.El-Saidy Ahmed I.Yousif Department of Animal Production Faculty of AgricultureMansoura UniversityMansoura35516Egypt Department of Veterinary Medicine Section of Obstetrics and GynecologyUniversity of SassariSassari07100Italy Animal Production Research Institute Agriculture Research CentreMinistry of AgricultureEgypt

Objective:To test the effects of extenders and packaging methods on morphological and functional characteristics of frozen thawed Ossimi ram ***:Ram semen was pooled,diluted in 3 different extenders:Tris-egg yolk(TEY),Tris-soybean lecithin(TSBL),and Tris-butylatedhydroxytoluene(TBHT),equilibrated at 5℃for 4 h,and packaged in straws or pellets for *** was evaluated for sperm progressive motility,viability,abnormality,and membrane integrity after dilution,equilibration and *** percentages of viable,early apoptotic,apoptotic,and necrotic spermatozoa as well as comet assay parameters were determined in post-thawed *** antioxidants capacity,malondialdehyde and lactic dehydrogenase were assayed in thawed seminal ***:After equilibration,only sperm membrane integrity was significantly higher(P<0.05)in TEY and TSBL than in *** thawing,TEY or TSBL in straws significantly improved sperm progressive motility and vitality(P<0.05).In thawed seminal plasma,TBHT or TSBL in straws and TBHT in pellets significantly reduced malondialdehyde,and TBHT in pellets significantly increased lactic dehydrogenase(P<0.05).TEY in straws increased viable sperm,while significantly decreased early apoptotic and apoptotic sperm(P<0.05).DNA damage was significantly decreased(P<0.05)in straws with TEY and TSBL,and tail moment decreased in straws with all extenders(P<0.05).Conclusions:Despite the disadvantages of dilution of cryopreserved semen with egg yolk,ram semen cryopreserved with TEY gives the best physical,morphological and functional characteristics in straws compared with pellets,followed by semen diluted with ***,semen diluted with TBHT or TSBL,regardless of packaging method,showed the highest impact on antioxidant status of cyopreserved ram semen.

关键词： Cryopreservation Egg-yolk Lecithin Sperm Ossimi ram

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