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Open Journal of Medical Microbiology 2013年第3期3卷 172-177页

作者： Xing Tang Steven Wood larissa baeva Division of biology OSEL/CDRH/FDA

A modified low denaturing temperature PCR (LDT-PCR) method combined with DNA microarray technique is developed in our lab for quick and effective identification of various mutations in an 81 base pair region of Mycobacterium Tuberculosis (MTb) ribosome RNA polymerase subunit b (rpob) gene associated with rifampin resistance. by incurporation of wild type (wt) allele fragments that had been PCR amplified previously, the target PCR fragments coming from mutant clinical MTb samples were codenaturized with incorporated wt type allele fragment at 94°C and then let them randomly form matched structures (homoduplex) and allele mismatch-containing structures (heteroduplex), respectively, when the temperature cooled down to 70°C. After the temperature was raised to 80°C, the heteroduplex double stranded fragments were preferentially denatured and resulted in PCR amplification as well as fluorescence incurporation. Since the homoduplex fragments need a higher temperature to be denatured, they were kept in double-stranded status at that temperature and failed to be PCR amplified. by hybridization of LDT-PCR products with the probes spotted on microarray slides, the fluorescent signals representing the presence of gene mutations were detected. We have tested this method on 35 clinical MTb samples and obtained satisfied results.

关键词： Low Denaturing Temperature PCR (LDT-PCR) Mycobacterium Tuberculosis (MTb) Ribosome RNA Polymerase Subunit b (rpob) Drug Resistance Homoduplex Heteroduplex Microarray

Field testing innovative differential geospatial and photogrammetric monitoring technologiesin mountainous terrain near Ashcroft,british Columbia,Canada

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Journal of Mountain Science 2021年第1期18卷 1-20页

作者： David HUNTLEY Peter bObROWSKY Roger MACLEOD Robert COCKING Jamel JOSEPH Drew ROTHERAM-clarke Geological Survey of Canada 1500-605 Robson StreetVancouverBritish ColumbiaV6B 5J3Canada Geological Survey of Canada 9860 West Saanich RoadSidneyBritish ColumbiaV8L 4B2Canada

This paper presents a novel approach to continuously monitor very slow-moving translational landslides in mountainous terrain using conventional and experimental differential global navigation satellite system(d-GNSS)technologies.A key research question addressed is whether displacement trends captured by a radio-frequency“mobile”d-GNSS network compare with the spatial and temporal patterns in activity indicated by satellite interferometric synthetic aperture radar(InSAR)and unmanned aerial vehicle(UAV)*** testing undertaken at Ripley Landslide,near Ashcroft in south-central british Columbia,Canada,demonstrates the applicability of new geospatial technologies to monitoring ground control points(GCPs)and railway infrastructure on a landslide with small and slow annual displacements(by way of deep-penetrating tension cracks at the head scarp and across the main slide *** over the last decade provides vital information for government agencies,national railway companies,and other stakeholders to understand geohazard risk,predict landslide movement,improve the safety,security,and resilience of Canada’s transportation infrastructure;and reduce risks to the economy,environment,natural resources,and public safety.

关键词： Landslide Change detection monitoring Global Navigation Satellite System Real-Time Kinematic System GeocubeTM bathymetric Survey Unmanned Aerial Vehicle Interferometric Synthetic Aperture Radar

Analysis of the Impact of Different PEV battery Chargers during Faults

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Journal of Power and Energy Engineering 2014年第8期2卷 31-44页

作者： Andrew D. clarke Himanshu A. bihani Elham b. Makram Keith A. Corzine Department of Electrical and Computer Engineering Clemson University Clemson USA

With a high penetration of Plug-In Electric Vehicles (PEVs) in the electric grid, utilities will have to face the challenges related to them. Considerable research is being done to study and mitigate the impact of PEVs on the electric grid and devise methodologies to utilize them for energy storage and distributed generation. In this paper, the impact of PEVs in a smart car park, placed in an unbalanced distribution system, during a Single Line to Ground fault with auto-recloser operation is studied. Level-2, bidirectional battery chargers with Current Controlled and Voltage Controlled Voltage Source Converters are modeled for the battery charging systems of the PEVs. A smart car park, with 16 vehicles connected to each of the three phases is simulated at one of the buses in the IEEE 13 bus Test Feeder. The impacts observed during the fault are analyzed and a method to mitigate them is suggested.

关键词： Electric Vehicles battery Chargers Voltage Source Converters Current Control Voltage Control Power Distribution Faults

Minimal improvement in coronary artery disease risk prediction in Chinese population using polygenic risk scores:evidence from the China Kadoorie biobank

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Chinese Medical Journal 2023年第20期136卷 2476-2483页

作者： Songchun Yang Dong Sun Zhijia Sun Canqing Yu Yu Guo Jiahui Si Dianjianyi Sun Yuanjie Pang Pei Pei Ling Yang Iona YMillwood Robin GWalters Yiping Chen Huaidong Du Zengchang Pang Dan Schmidt Rebecca Stevens Robert clarke Junshi Chen Zhengming Chen Jun Lv Liming Li On behalf of the China Kadoorie biobank Collaborative Group Department of Epidemiology and biostatistics School of Public HealthPeking UniversityBeijing 100191China Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Peking University Center for Public Health and Epidemic Preparedness&Response Beijing 100191China Fuwai Hospital Chinese Academy of Medical Sciences Beijing 100730China Medical Research Council Population Health Research Unit at the University of Oxford Oxford OX37LFUK Clinical Trial Service Unit and Epidemiological Studies Unit(CTSU) Nuffield Department of Population HealthUniversity of OxfordOxford OX37LFUK Qingdao Center of Disease Control and Prevention QingdaoShandong 266033China China National Center for Food Safety Risk Assessment Beijing 100738China 不详

background:Several studies have reported that polygenic risk scores(PRSs)can enhance risk prediction of coronary artery disease(CAD)in European ***,research on this topic is far from sufficient in non-European countries,including *** aimed to evaluate the potential of PRS for predicting CAD for primary prevention in the Chinese ***:Participants with genome-wide genotypic data from the China Kadoorie biobank were divided into training(n=28,490)and testing sets(n=72,150).Ten previously developed PRSs were evaluated,and new ones were developed using clumping and thresholding or LDpred *** PRS showing the strongest association with CAD in the training set was selected to further evaluate its effects on improving the traditional CAD risk-prediction model in the testing *** risk was computed by summing the product of the weights and allele dosages across genome-wide single-nucleotide *** of the 10-year first CAD events was assessed using hazard ratios(HRs)and measures of model discrimination,calibration,and net reclassification improvement(NRI).Hard CAD(nonfatal I21-I23 and fatal I20-I25)and soft CAD(all fatal or nonfatal I20-I25)were analyzed ***:In the testing set,1214 hard and 7201 soft CAD cases were documented during a mean follow-up of 11.2 *** HR per standard deviation of the optimal PRS was 1.26(95%CI:1.19-1.33)for hard *** on a traditional CAD risk prediction model containing only non-laboratory-based information,the addition of PRS for hard CAD increased Harrell’s C index by 0.001(-0.001 to 0.003)in women and 0.003(0.001 to 0.005)in *** the different high-risk thresholds ranging from 1%to 10%,the highest categorical NRI was 3.2%(95%CI:0.4-6.0%)at a high-risk threshold of 10.0%in *** association of the PRS with soft CAD was much weaker than with hard CAD,leading to minimal or no improvement in the soft CAD ***:In this Chinese population sam

关键词： Coronary artery disease Polygenic risk score Risk prediction model Chinese population

The Distribution and Substrate Specificity of Extracellular Nuclease Activity in Marine Fungi

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Open Journal of Marine Science 2012年第4期2卷 188-195页

作者： larissa A. balabanova Michael V. Pivkin Valery A. Rasskazov G.b. Elyakova Pacific Institute of bioorganic Chemistry Far-Eastern Branch of Russian Academy of Sciences Vladivostok Russia

The distribution and specificity of extracellular nucleases produced by marine fungi belonging to eleven genera, namely: Alternaria, Aspergillus, Aureobasidium, Chaetomium, Fusarium, Gliomastix, Humicola, Penicillium, Scopulariopsis, Wardomyces, Periconia, have implied its important function in the organic phosphorus and nitrogen circle in the Ocean. The fungal nucleases of 64 isolates tested were more or less specific for single-stranded DNA with a high preferential specificity towards poly-U substrate with forming of 5’-phosphate mononucleotides. A couple of the nucleases were capable of RNA digesting. The highest level of extracellular nucleolytic ability was observed in Penicillium spp. isolates. The tight correlation found between extracellular nuclease activity and the rate of thymidine uptake by actively growing and sporulating marine fungus Penicillium melinii suggests that this nuclease is required for fulfilling the nucleotide pool of precursors of DNA biosynthesis during transformation of hyphae into the aerial mycelium and conidia in stressful environmental conditions.

关键词： Marine Fungi Marine Environment Single-Strand-Specific Nuclease DNAse RNAse ssDNA Thymidine Uptake

Endoscopic balloon catheter dilatation via retrograde or static technique is safe and effective for cricopharyngeal dysfunction

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World Journal of Gastrointestinal Endoscopy 2017年第4期9卷 183-188页

作者： Vinay Chandrasekhara Joyce Koh Lakshmi Lattimer Kerry b Dunbar William J Ravich John O clarke Division of Gastroenterology and Hepatology Department of Internal Medicinethe Johns Hopkins Medical Institutions Gastroenterology Division Department of Internal MedicineUniversity of Pennsylvania Health System Perelman Center for Advanced Medicine South Pavilion Gastroenterology and Liver Diseases Depart-ment of Internal Medicinethe George Washington University Division of Gastroenterology and Hepatology Department of MedicineUniversity of Texas Southwestern Medical SchoolDallas VA Medical Center

AIM To evaluate the safety and efficacy of upper esophageal sphincter(UES) dilatation for cricopharyngeal(CP) dysfunction. To determine if:(1) indication for dilatation; or(2) technique of dilatation correlated with symptom *** All balloon dilatations performed at our institution from over a 3-year period were retrospectively analyzed for demographics, indication and dilatation site. All dilatations involving the UES underwent further review to determine efficacy, complications, and factors that predict success. Dilatation technique was separated into static(stationary balloon distention) and retrograde(brusque pull-back of a fully distended balloon across the UES).RESULTS Four hundred and eighty-eight dilatations were reviewed. Thirty-one patients were identified who underwent UES dilatation. Median age was 63 years(range 27-81)and 55% of patients were male. Indications included dysphagia(28 patients), globus sensation with evidence of UES dysfunction(2 patients) and obstruction to echocardiography probe with cricopharyngeal(CP)bar(1 patient). There was evidence of concurrent oropharyngeal dysfunction in 16 patients(52%) and a small Zenker's diverticula(≤ 2 cm) in 7 patients(23%). Dilator size ranged from 15 mm to 20 mm. Of the 31 patients, 11 had dilatation of other esophageal segments concurrently with UES dilatation and 20 had UES dilatation ***-up was available for 24 patients for a median of 2.5 mo(interquartile range 1-10 mo), of whom 19 reported symptomatic improvement(79%). For patients undergoing UES dilatation alone, follow-up was available for 15 patients, 12 of whom reported improvement(80%).Nineteen patients underwent retrograde dilatation(84%response) while 5 patients had static dilatation(60%response); however, there was no significant difference in symptom improvement between the techniques(P = 0.5).Successful symptom resolution was also not significantly affected by dilator size, oropharyngeal dysfunction,Zenker's diverticu

关键词： Cricopharygeal 机能障碍 Cricopharyngeal 酒吧咽下困难食道的膨胀内视镜的汽球膨胀

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Jean baudrillard

Jean Baudrillard

Diabetes Mellitus, Leprosy: Intensive Education Protocol in Foot at Risk, Santa Marcelina Hospital, Amazon, brazil

作者： (interviewee..1929-2007 )baudrillard Jean (editor.)(Lecturer in human geography) ·Smith Richard G. (editor..1964-)clarke David b.

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Open Journal of Endocrine and Metabolic Diseases 2017年第1期7卷 52-58页

作者： Orlando Leite Vanderneide Oliveria Augiceli Rodrigues Ticiana Albuquerque Alexandre Carvalho larissa Gaspar Alexandre Falqueti larissa bianca Paiva Cunha de Sá Alberto Krayyem Arbex Division of Endocrinology Santa Marcelina Hospital Porto Velho Brazil Division of Endocrinology IPEMED Medical School (IPEMED) S&atilde o Paulo Brazil

Objective: To apply an intensive and multidisciplinary education protocol in order to decrease, improve, delay or cancel the beginning of neuropathy and the manifestation of lesions in diabetic patients. Methodology: This is a cross-sectional descriptive study carried out at the Diabetes Mellitus Outpatient Clinic and Ward of Santa Marcelina Hospital in Porto Velho. This research was based on cases of patients with Diabetes and Diabetics with Leprosy. The criteria used to include the patients were: being treated with insulin therapy, not to be amputated, being on high medication of the leprosy at the moment of the evaluation and to present nutritional risk classification by the screening. The population was divided into two groups of fifteen patients: eight diabetic patients, four male and four female. Seven diabetics associated with leprosy were 4 males and 3 females. The monofilament test (Semmes-Weinstein 10 g) was used as the classification basis. Results and Discussion: Group A, called the intensive care group, began diabetes education work with medical, nutritional and rehabilitation guidelines by a multidisciplinary team for an average period of 15 days in the ward and after discharged with biweekly monitoring. Group b, called conventional care, received the same guidelines in outpatient care and the monitoring followed the quarterly protocol. both groups were evaluated and reassessed for a period of 180 days in the outpatient clinic. Group A consisted of 8 (100%) patients, 4 (50%) diabetics and 4 (50%) diabetics and leprosy patients. Conclusion: Intensive education in diabetes showed an improvement in the sensitivity, healing and nutritional status of the patients, leading to an improvement in quality of life and disability level, reducing or delaying the beginning of neurological complications. The protocol intensive method demonstrated a 100% improvement in patients in group A.

关键词： Continuing Education Foot at Risk Healing

bright spots for inland fish and fisheries to guide future hydropower development

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Water biology and Security 2022年第1期1卷 81-99页

作者： William M.Twardek Ian G.Cowx Nicolas W.R.Lapointe Craig Paukert T.Douglas beard Elena M.bennett David browne Andrew K.Carlson Keith D.clarke Zeb Hogan Kai Lorenzen Abigail J.Lynch Peter b.McIntyre Paulo Pompeu Mark Rogers Alexis Sakas William W.Taylor Taylor D.Ward Zeenatul basher Steven J.Cooke Fish Ecology and Conservation Physiology Laboratory Department of Biology and Institute for Environmental StudiesCarleton University1125 Colonel By DrOttawaON K1S 5B6Canada Fisheries Institute at the University of Hull Cottingham RdHullHU67RXUnited Kingdom Canadian Wildlife Federation 350 Michael Cowpland DrKanataONK2M 2W1Canada U.S.Geological Survey Missouri Cooperative Fish and Wildlife Research UnitUniversity of MissouriColumbiaMO65211United States U.S.Geological Survey National Climate Adaptation Science Center12201 Sunrise Valley DriveMS-516RestonVA20192United States McGill School of Environment McGill University845 Sherbrooke St WMontrealQuebec H3A 0G4Canada Princeton Environmental Institute and Department of Ecology and Evolutionary biology Princeton UniversityM30 Guyot HallPrincetonNJ08544United States Ecological Sciences Section Science branch Fisheries and Oceans CanadaCanadaPO Box/CP 5667St.John'sNewfoundland and LabradorA1C 5X1Canada University of Nevada Reno.4505 S Maryland PkwyLas VegasNV89154United States Fisheries and Aquatic Sciences School of Forest Resources and ConservationUniversity of Florida7922 NW 71st StreetGainesvilleFL32653United States Department of Natural Resources Cornell UniversityIthacaNY14853United States Departamento de biologia Universidade Federal de LavrasAquenta SolLavrasState of Minas Gerais37200-900Brazil U.S.Geological Survey Tennessee Cooperative Fishery Research UnitTennessee tech UniversityCookevilleTN38505United States The Nature Conservancy 6375 Riverside DriveSuite 100DublinOH43017United States Michigan State University Department of Fisheries and WildlifeEast LansingMI48823United States

Hydropower production is one of the greatest threats to fluvial ecosystems and freshwater *** that we have entered the Anthropocene,there is an opportunity to reflect on what might constitute a‘sustainable’Anthropocene in the context of hydropower and riverine fish *** elements of existing practices that promote favorable social-ecological outcomes(i.e.,‘bright spots’)is timely given that there are plans to expand hydropower capacity in previously undammed rivers,intensify dam development in some of the world's largest river systems,and re-license existing *** approach this from a pragmatic perspective:for the foreseeable future,hydropower will likely remain an important source of renewable *** offer support for moving toward a more‘sustainable’Anthropocene,we provide syntheses of best practices during the siting,design,construction,operation,and compensation phases of hydropower development to minimize impacts on inland *** each phase,we offer positive examples(or what might be considered‘bright spots’)pertaining to some of the approaches described within our syntheses,acknowledging that these projects may not be viewed as without ecological and(or)societal detriment by all *** findings underscore the importance of protecting critical habitat and free-flowing river reaches through careful site selection and basinscale planning,infrastructure designs that minimize reservoir effects and facilitate safe passage of fish,construction of hydropower plants using best practices that minimize long-term damage,operating guidelines that mimic natural flow conditions,and compensation that is lasting,effective,inclusive,and locally *** from these‘bright spots’may require engagement of diverse stakeholders,professionals,and governments at scales that extend well beyond a given site,river,or even ***,environmental planning that integrates hydropower development into broader discussions

关键词： Migration Dams Salmon Hydroelectric Flow Socioecological Fish passage barriers Ecosystems

Nonsmooth Semi-Infinite Minmax Programming Involving Generalized(Φ, ρ)-Invexity

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Journal of Systems Science & Complexity 2015年第4期28卷 857-875页

作者： UPADHYAY b b MISHRA S K Department of basic Sciences & Humanities National Institute of Technology

This paper introduces some new generalizations of the concept of(Φ,ρ)-invexity for nondifferentiable locally Lipschitz functions using the tools of clarke *** functions are used to derive the necessary and sufficient optimality conditions for a class of nonsmooth semi-infinite minmax programming problems,where set of restrictions are indexed in a compact *** the sufficient optimality conditions,the authors formulate three types of dual models and establish weak and strong duality *** results of the paper extend and unify naturally some earlier results from the literature.

关键词：规划问题局部Lipschitz函数半无限非光滑极大极小最优性条件广义凸性