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American Journal of Plant Sciences 2015年第1期6卷 240-248页

作者： Essé Agossou Mélanie Ahokpe Justin Behanzin Marcellin Takin Ganiou Yessoufou Loica Zohoun lamine baba-mous.a Alphonse Sezan Laboratoire de Biomembranes et Signalisation Cellulaire Département de Physiologie Animale Université d’Abomey-Calavi Cotonou Benin Laboratoire de Biologie et de Typage moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Université d’Abomey Calavi Cotonou Benin

The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD) were treated with the extract in oral doses of respectively 10, 25 and 50 mg/kg body weight for 30 days;three other groups receiving respectively these doses of the extract were placed under a priori temporary overload of glucose;a control group received instead distilled water treatment. Blood glucose, triglycerides and total cholesterol were measured by spectrophotometry and weight gain of the animals was performed at the beginning of the experiment and every 2 weeks during the study period. The phytochemical screening revealed the presence of polyphenolic compounds (majority) of saponins of anthracene derivatives and steroids in the extract. The test results indicate that under the dose of 50 mg/kg body weight (BW) there was a significant decrease (p < 0.05) in blood glucose after 24 hours. The extract had no effect on the lipid profile or body weight of rabbits.

关键词： Rabbit Khaya senegalensis Glucose Lipid Profile

Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers

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American Journal of Molecular Biology 2016年第1期6卷 12-24页

作者： Hafiz A. Salami Kamirou Chabi Sika Wilfrid Padonou Djima Aly Chabi Yallou Adolphe Adjanohoun Simeon Kotchoni lamine baba-mous.a Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Programme de Technologie Agricole (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Bénin Centre de Recherches Agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Cotonou Bénin Centre de Recherches Agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) Ina Bénin Departement of Biology Rutgers University Camden USA

Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize inbred lines. The aim of this study was to evaluate the genetic diversity of Benin’s maize accessions by SSR marker. Thus, one hundred eighty seven maize accessions from three areas (South, Center and North) were analyzed using three SSR markers. A total of 227 polymorphic bands were produced and showed high genetic diversity (Shannon index = 0.51). The polymorphic information content (PIC) values for the SSR loci ranged from 0.58 to 0.81, with an average of 0.71. Genetic distance-based UPGMA dendrogram showed a genetic differentiation between accessions and they were grouped into four clusters in each area. This work provides necessary information that can be used not only to improve the maize production and conservation but also to better manage genetic species resources in Benin.

关键词： SSR PCR Molecular Characterization LZea mays L. Benin

Characterization of Potential Pathogenic Bacteria Isolated in High-Risk Infectious Services at the University Hospital Center of Suru-Léréin Benin

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Advances in Microbiology 2021年第1期11卷 1-15页

作者： Cyriaque Dégbey Rosine Olga Quenum Wassiyath mousse Haziz Sina Akim Socohou Eric Hounsinou lamine baba-mous.a Honoré Bankolé Regional Institute of Public Health University of Abomey-Calavi Ouidah Benin University Clinic of Hospital Hygiene National Hospital and University Center Hubert Koutoukou MAGA Cotonou Benin Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin Laboratory of Biology and Molecular Typing in Microbiology Abomey-Calavi Benin University Hospital Center of Suru-Léré Cotonou Benin

Healthcare-associated infections (HCAIs) remain a major public health concern. The aim of this study was to characterize potential pathogenic bacteria isolated in high-risk infectious services at the University Hospital Center of Suru-Léré in Benin. A cross-sectional, descriptive study was carried out on 215 samples with 8 air samples, 20 hand samples and 187 samples of medical-technical material. Genes for resistance to β-lactam antibiotics (blaTEM, blaSHV, blaCTX-M and blaOXA-1) were sought. The data were processed and analyzed using EPI Info version 3.5.4 software. A total of 10 bacterial species were identified. Of the 319 bacterial strains identified, Staphylococcus saprophyticus, Staphylococcus aureus and Enterococcus faecalis were predominant at 20.06%, 17.87% and 11.28% respectively. Antibiotic susceptibility showed that the majority of bacterial strains were multidrug-resistant. Of the 4 resistance genes sought, only the blaTEM and blaSHV genes were found. The poor microbiological quality observed in high-risk infectious services could be a risk factor for healthcare-associated infections. It is therefore useful to implement preventive measures in order to prevent or reduce the risk of healthcare-associated infections.

关键词： Bacteria Infection Risk Factors Benin

Survey of Cereal Consumption Habits in the Community of Djougou, Benin

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Food and Nutrition Sciences 2023年第9期14卷 843-863页

作者： Mouhamed N. El-Hadji Alassane Moutawakilou J. Kisito Chabi-Sika Agossou D. P. Noumavo Haziz Sina Joseph Dossou lamine baba-mous.a Farid baba-mous.a Laboratoire de Microbiologie et des Technologies Alimentaires Faculté des Sciences et Techniques Université d’Abomey-Calavi Abomey-Calavi Bénin Agro Sciences Laboratory (ASL) Abomey-Calavi Bénin Agence Béninoise de Sécurité Sanitaire des Aliments Ministère de l’Agriculture de l’Elevage et de la Pèche Cotonou Bénin Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Faculté des Sciences et Techniques Université d’Abomey-Calavi Abomey-Calavi Bénin Laboratoire de Bioingénierie des Procédés Alimentaires Faculté des Sciences Agronomiques Université d’Abomey-Calavi Abomey-Calavi Bénin

The cereal group occupies a prominent place in the dietary habits of people in northern Benin and there is little recent information on cereal consumption. This study aims to assess the consumption, acquisition and supply of cereals to households in the community of Djougou. A semi-directive survey with KoBoCollect was conducted among 369 households to collect individual cereal food consumption data. The survey data processed by statistical tools showed that the most consumed cereals are maize (95%, p = 0.887), millet (58%, p = 0.755), rice (55%, p = 0.753), sorghum (15%, p = 0.635), wheat (5%, p = 0.920) and fonio barely 5%. The most common mode of acquisition in Djougou is purchase (50%, p = 0.947) but donation is also observed (25%, p = 0.988) as well as production observed in 20.6% of households. Purchases are made from retailers in local markets (45%, p = 0.920) but also in streets and alleys (30%, p = 0.765). The most widely used preservation technique is drying at room temperature (70%, p = 0.995). Households most often dry in the areas provided in the field (50%, p = 0.783) and at home (40%, p = 0.643). The preferred storage location is the kitchen (60%, p = 0.790). The bedroom (20%, 0.669) and the store (15%, 0.522) are the alternative places for storing cereals. In addition, the supply costs of cereals increased between 2020 and 2021. This vertiginous rise in prices is due, among other things, to the covid19 pandemic. The various data generated not only make it possible to have fresh data but also to invest them in the assessment of health risks for the achievement of a high level of protection of the health and life of consumers.

关键词： Cereals Survey Consumption Data Food Safety Benin

Assessment of Treatment from the National Laboratory of Cholera Patients in Benin: Retro-Prospective and Prospective Study

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Advances in Microbiology 2020年第9期10卷 443-455页

作者： Bawa Boya Théodora A. Ahoyo Victorien T. Dougnon Haziz Sina Odilon Nonfodji Wassiyath moussé Adolphe Adjanohoun lamine baba-mous.a Laboratoire de Biologie et de Typage Moléculaire en Microbiologie (LBTMM). University of Abomey-Calavi Cotonou Benin Génie de Biologie Humaine/Ecole Polytechnique d’Abomey-Calavi (EPAC) University of Abomey-Calavi Cotonou Benin Unité de Recherche en Microbiologie Application et Pharmacologie des substances Naturelles (URMAPha) University of Abomey-Calavi Cotonou Benin Institut National des Recherches Agricoles du Benin Cotonou Benin

Cholera is re-emerging as an infectious disease with an increase in its overall incidence in Africa since 2008. To analyze the epidemiological aspects of the cholera outbreak in endemic areas in Benin. The study is a retrospective investigation on 2560 data of cholera patients received in health facilities from 2012 to 2016 in Benin. Besides, a prospective study was conducted. This prospective study includes 513 person (health professionals, community workers and people exposed to cholera) of five different areas of the country. It was conducted from April 10 to May 10, 2017. A survey was conducted over 513 in order to collect data on cholera symptoms, hygiene guidelines suggested by the respondents, excreta disposal, drinking water, household waste management and direct observation. Software R 3.4.0 was used for simple correspondence factor analysis (CFA). From 2012 to 2016, 16.48% of samples were taken and 48.34% were positive for Vibrio cholera O1, El Tor biotype, Ogawa serotypes. The Littoral area is leading in the number of cases (33.83%) followed by Atlantic (23.75%), Ouémé (16.79%) and Colline (8.91%). The survey revealed that the exposed populations are unaware of the symptoms of cholera and do not perceive the quality of drinking water as a strong guidance in the prevention of cholera. Nevertheless, the majority of respondents (86%) are aware for the best times to wash their hands and know that they need to go to hospital in case of symptoms. Our study highlights the persistence of vulnerability factors to cholera in the targeted population, despite a good knowledge of prevention rules among staff and the population.

关键词： Cholera Hygiene Guidelines Practices and Preventions Benin

Chilli Pepper (<i>Capsicum</i>Spp.) Diversity, Production and Fungal Contamination Management in Benin

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American Journal of Plant Sciences 2021年第12期12卷 1859-1879页

作者： Nicéphore M. Glodjinon Agossou P. Noumavo Brice A. M. Ohin Noel S. Tovide Fatiou Toukourou lamine baba-mous.a Aly Savadogo Farid baba-mous.a Laboratoire de Microbiologie et des Technologies Alimentaires Faculté des Sciences et Techniques Université d’Abomey-Calavi Abomey-Calavi Bénin Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Faculté des Sciences et Techniques Université d’Abomey-Calavi Abomey-Calavi Bénin Laboratoire de Biochimie et Immunologie Appliquée Unité de Formation et de Recherche en Sciences de la Vie et de la Terre Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso

The present study was carried out in the form of a survey with the aim of listing and describing the technical itineraries of production, storage and conservation used in six departments of high production of chilli pepper in Benin. The survey was conducted on the basis of a questionnaire in the six departments of Benin: Ouémé, Plateau, Atlantic, Littoral, Zou and Collines. The survey collected information on the socio-economic characteristics of producers, the varieties of chilli pepper grown, and the current technical itineraries used for the production, storage and conservation of chilli pepper. On the other hand, it also gave an idea of the constraints that prevent the production of chilli pepper of good sanitary quality. The results of this survey show that three varieties of chilli peppers are the most produced and consumed in Benin. These are the Gbotakin variety (Capsicum chinense), the Afundja variety (Capsicum annuum) and the Danhomètakin variety (Capsicum frutescens). The technical itineraries related to the cultivation of these chilli peppers varieties are the same in the six study departments. Unfortunately, their contamination sometimes starts at the nursery stage, passing through the field where other contaminants are added and develop until harvest. After harvest, contamination can continue in storage and finally reach the consumer if proper measures are not taken. The inappropriate or inadequate use of phytosanitary products in the chilli pepper industry is also a source of illness for consumers of this vegetable fruit. Training of producers on the best technical itineraries for the production and storage of chilli peppers is essential to revitalize and develop this sector by improving the sanitary quality of the chilli peppers produced in Benin.

关键词： Chilli Pepper Capsicum Spp. Technical Itineraries Fungal Contamination Benin

Morphological Diversity of Corn’s (<i>Zea mays</i>L.) Local Cultivar and Improved Varieties in Central and North of Benin

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American Journal of Plant Sciences 2015年第18期6卷 2867-2877页

作者： Hafiz A. Salami Adolphe Adjanohoun Wilfrid Padonou Abdoul-Madjidou Yacoubou Djima Aly Chabi Yallou Haziz Sina lamine baba-mous.a Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Centre de Recherches Agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Attogon Benin Programme Technologie Agricole et Alimentaire (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Benin Centre de Recherches Agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) N’dali Benin

The better knowledge of a plant genetic biodiversity is based on a prior study of its agro-morphological characteristics. The objective of this study was to investigate the morphological diversity of maize accessions and its structure on the basis of 14 variables. For the experimentation, 43 and 98 maize accessions were planted in three replicates respectively in central and northern Benin following an incomplete randomized block. The mixed model analysis of two factors variance revealed a significant difference for all accessions considering each agro-morphological characteristic evaluated except the germination day of two areas seed accessions. The numerical classification grouped the accessions into four groups in each zone. The stepwise discriminant analysis showed that early characters related to plant height and ear insertion were the variables that discriminated accessions in both zones. Maturity and recovery ears, sensitivity to streak and the germination days are the variables that discriminate accessions of the two areas. These results provide a database for the creation of improved maize varieties that meet the needs of

关键词： Accessions Characterization Agro-Morphological Zea mays L. Benin

Diversity, Chemical Compositions and Beneficial Effects of Some Spices and Aromatic Leaves Consumed in Benin and in the World: Critical Review

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American Journal of Plant Sciences 2023年第5期14卷 569-598页

作者： Carole Vikou Josiane Semassa Zoriţa Diaconeasa Gautier Roko Majoie Tohoyessou Durand Dah-Nouvlessounon Haziz Sina Andreea Stanilă lamine baba-mous.a Laboratory of Biology and Molecular Typing in Microbiology Faculty of Science and Technology University of Abomey-Calavi Abomey-Calavi Benin Republic Faculty of Food Science and Technology University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca Cluj-Napoca Romania

Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and aromatic herbs, the chemical composition, the different properties and forms of use of six spices and aromatic herbs commonly used in Benin and around the world. These are Zingiber officinalis (ginger), Curcuma longa (curcuma), Syzygium aromaticum (clove) and three aromatic herbs Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), and Laurus nobilis (laurel). The methodology used is that of documentary research oriented towards the consultation of previous scientific documents that have highlighted the different pharmacological activities of the different species of spices and aromatic plants targeted. It is important to note that more than twenty plant species are used as spices and aromatic plants in Benin and around the world. Chemically, these different spices and aromatic herbs contain certain secondary metabolites such as flavonoids, tannins, coumarins, alkaloids, steroids, terpenes, saponins, and polyphenols. This diversity of secondary metabolites alone or in a possible synergy may be responsible for many beneficial properties attributed to spices and aromatic herbs.

关键词： Spices Aromatic Leaves Pharmacological Effects Humans

Status of Techniques Used to Control Moulds in Maize Storage in Africa

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Agricultural Sciences 2022年第1期13卷 49-64页

作者： Halfane Lehmane Rafiatou Ba Durand Dah-Nouvlessounon Haziz Sina Gautier Roko Farid T. Bade Akim Socohou Adolphe Adjanohoun lamine baba-mous.a Laboratory of Biology and Molecular Typing in Microbiology Department of Biochemistry and Cell Biology Faculty of Sciences and Techniques University of Abomey-Calavi Cotonou Benin Institut National des Recherches Agricoles du Bénin Cotonou Benin

In Benin, the preservation of maize is a real problem involving important physical and chemical properties losses. Physical losses are due by rodents, pests and insects and chemical losses are mainly due to fungal infection. Several methods, products and practices are used by farmers to overcome these problems. The methods used do not always control the losses caused by fungal infection. The objective of the study was to make a bibliographic synthesis of the impacts induced by the items, practices and methods used to overcome the chemical losses of maize. In the search for information, the library of the Ministry of Agriculture, Livestock and Fisheries was consulted;the archives of the library of the University of Abomey-Calavi and the National Institute of Agricultural Research of Benin were also consulted. Thus, chemicals such as sofagrain, Pyrimiphos-Methyl, Thiamethoxam, Pyrimiphos-Methyl, Permethrin, Deltamethrin, fumigant are used to control physical attacks on maize. Ash and plants species such as neem tree (Azadirachta indica) are generally used for the preservation and storage of maize seeds. In addition, chemicals such as arsenious anhydride and zinc phosphide are used for the preservation of corn. The use of these molecules and chemical products has negative impacts on human health and the environment. Of all the methods used, no treatment is still popularized for the reduction of the chemical losses caused by the fungal infection in corn storage.

关键词： Impact Aflatoxins Cereals Conservation Africa

Case-by-Case Surveillance for Bacterial Meningitis in Benin: Data Analysis, 2016 to 2018

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Open Journal of Epidemiology 2020年第1期10卷 1-14页

作者： Togbemabou Primous Godjedo moussiliou Noel Paraiso Alidehou Jerrold Agbankpe Tamegnon Victorien Dougnon Cyriaque Degbey Angele Ahoyo lamine baba-mous.a HonoréBankole Epidemiological Surveillance Service Ministry of Public HealthCotonouBenin National Health Laboratory Ministry of Public HealthCotonouBenin Regional Institute of Public Health University of Abomey-CalaviCotonouBenin Research Unit in Applied Microbiology and Pharmacology of Natural Substances Research Laboratory in Applied BiologyPolytechnic School of Abomey-CalaviUniversity of Abomey-CalaviCotonouBenin [Laboratory of Biology and Molecular Typing in Microbiology Faculty of Science and TechnologyUniversity of Abomey-CalaviUACCotonouBenin

Background: Bacterial meningitis is an inflammation of the meninges caused mainly by three bacterial species Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae that are transmitted by nasopharyngeal secretions emitted by carriers. Meningitis is a public health problem in Benin, like all countries in the African meningitis belt. This study aims to analyze the epidemiological surveillance data of meningitis in Benin from 2016 to 2018. Methods: Each suspect case of meningitis was recorded and Cerebrospinal Fluid (CSF) samples were collected. CSF collection was accompanied by the Integrated Disease Surveillance and Response form. This sheet provides information on the patient’s social-demographic and epidemiological data. CSF specimens were sent to the laboratory for analysis and identification (Gram stain, biochemical parameters, and latex agglutination test) of pathogens according to the WHO standards. Results: Of the 2992 patients with suspected meningitis, 2893 were hospitalized with a death rate of 9.4% (281/2992). The sex ratio of registered patients was 1.29 in favor of men. The median age was 4 years (min: 0;max: 90). Patients younger than five years were the most represented (44.8%). During the study period, there was a decrease in the incidence of meningitis per 100,000 inhabitants (6.3 to 3.2 from 2016 to 2018). Of 2928 CSF samples collected we were able to identify 899 pathogenic bacterial species. The most represented species are S. pneumoniae (63.4%), N. meningitidis (24.4%) and H. influenzae (12.2%). Conclusion: The burden of disease is disproportionate in the northern departments as in others. The frequency of bacterial meningitis in the northern region increased during the study period. However, deaths have been recorded in the departments of the South (“Atlantic”, “Plateau”). This suggests an improvement in epidemiological surveillance and case management throughout the national territory.

关键词： Benin Meningitidis Epidemiologic Surveillance CSF Bacterial Meningitidis