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Enhancing the surface Properties of a Bioengineered Anterior Cruciate Ligament Matrix for Use with Point-of-Care stem Cell Therapy

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Engineering 2021年第2期7卷 153-161页

作者： Xiaohua Yu Paulos Y.Mengsteab Ganesh Narayanan lakshmi S.Nair Cato T.Laurencin Connecticut Convergence Institute for Translation in Regenerative Engineering University of Connecticut HealthFarmingtonCT 06030USA Raymond and Beverly sackler Center for Biological Physical and Engineering SciencesUniversity of Connecticut HealthFarmingtonCT 06030USA Department of Orthopedic surgery University of Connecticut HealthFarmingtonCT 06030USA Department of Biomedical Engineering University of ConnecticutStorrsCT 06269USA Department of Materials science and Engineering University of ConnecticutStorrsCT 06269USA Department of Chemical and Biomolecular Engineering University of ConnecticutStorrsCT 06269USA Department of Reconstructive sciences University of Connecticut HealthFarmingtonCT 06030USA

We have previously developed a poly(L-lactic)acid(PLLA)bioengineered anterior cruciate ligament(ACL)matrix that has demonstrated enhanced healing when seeded with primary ACL cells prior to implantation in a rabbit model,as compared with the matrix alone.This suggests that improving cell adhesion on the matrix may beneficially affect the healing response and long-term performance of the bioengineered ACL matrix.One regenerative engineering approach involves enhancing the surface properties of the matrix to support cell adhesion and growth in combination with point-of-care stem cell therapy.Herein,we studied the cell adhesion properties of PLLA braided microfiber matrices enhanced through the physical adsorption of fibronectin and air plasma treatment.We evaluated the kinetics and binding efficiency of fibronectin onto matrices at three time points and three fibronectin concentrations.Incubating the matrix for 120 min in a solution of 25 lgmL1 fibronectin achieved the greatest binding efficiency to the matrix and cellular adhesion.Exposing the matrices to air plasma treatment for 5 min before fibronectin adsorption significantly enhanced the cell adhesion of rabbit bone marrow-derived mesenchymal stem cells(R-BMMsCs)24 h post cell seeding.Finally,cellular proliferation was monitored for up to 21 d,the matrices were exposed to air plasma treatment,and fibronectin adsorption was found to result in enhanced cell number.These findings suggest that exposure to air plasma treatment and fibronectin adsorption enhances the cellular adhesion of PLLA braided microfiber matrices and may improve the clinical efficacy of the matrix in combination with point-of-care stem cell therapies.

关键词： Anterior cruciate ligament Ligament Poly(L-lactic)acid plasma treatment Fibronectin stem cells Adhesion

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The Quest toward limb regeneration:a regenerative engineering approach

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Regenerative Biomaterials 2016年第2期3卷 123-125页

作者： Cato T.Laurencin lakshmi S.Nair Department of Orthopaedic surgery Institute for Regenerative EngineeringRaymond and Beverly Sackler Center for BiomedicalBiologicalPhysical and Engineering SciencesUniversity of Connecticut HealthFarmingtonCT 06030USA Department of Biomedical Engineering University of ConnecticutStorrsCT 06269USA Department of Materials science and Engineering University of ConnecticutStorrsCT 06269USA Department of Chemical and Biomolecular Engineering University of ConnecticutStorrsCT 06269USA Department of Craniofacial sciences School of Dental MedicineUniversity of Connecticut HealthFarmingtonCT 06030USA.

The Holy Grail to address the clinical grand challenge of human limb loss is to develop innovative strategies to regrow the amputated limb.The remarkable advances in the scientific understanding of regeneration,stem cell science,material science and engineering,physics and novel surgical approaches in the past few decades have provided a regenerative tool box to face this grand challenge and address the limitations of human wound healing.Here we discuss the convergence approach put forward by the field of Regenerative Engineering to use the regenerative tool box to design and develop novel translational strategies to limb regeneration.

关键词： regenerative engineering limb regeneration stem cells advanced biomaterial science clinical translation

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A combination of learning and non-learning based method for enhancement, compression and reconstruction of underwater images

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Aquaculture and Fisheries 2022年第2期7卷 201-210页

作者： Rashmi s.nair sandanam Domnic Department of Computer Applications National Institute of TechnologyTiruchirappalliTamil Nadu620015India

Complex nature of underwater environment poses biggest challenge towards image acquisition and transmission of underwater images.This paper proposes an integrated approach which consists of a non-learning enhancement method with deep Convolutional Neural Networks(CNN)for compression and reconstruction of the image.The proposed method does color and contrast correction for image enhancement.The enhanced images are down-sampled using 9-layer CNN followed by Discrete Wavelet Transform(DWT).The decompression is done by using Inverse DWT.Further,the sub-pixel up-sampled image is de-blurred using a three-layer CNN.Residual Dense CNN(RD-CNN)is used to improve the quality of the reconstructed image after deblurring.The quality of the reconstructed images is measured using Peak signal to Noise Ratio(PsNR)and structural similarity Index Metric(ssIM).The proposed model provides better image enhancement,compression,and reconstruction quality than the existing state-of-the-art methods and super Resolution CNN(sRCNN)respectively.

关键词： Convolutional neural network Discrete wavelet transform Residual dense convolutional neural network Peak signal to noise ratio structural similarity index metric super resolution CNN

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structural,magnetic and antibacterial properties of manganese-substituted magnetite ferrofluids

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International Journal of Minerals,Metallurgy and Materials 2023年第7期30卷 1417-1426页

作者： Blessy Babukutty Deepalekshmi Ponnamma swapna s.nair Jiya Jose saritha G.Bhat sabu Thomas Department of Physics Central University of KeralaKasargod 671314India Materials science and Technology Program College of Arts and SciencesQatar UniversityP.O.Box 2713DohaQatar Department of Biotechnology Cochin University of Science and TechnologyKalamasseryKeralaIndia Department of Chemistry Mahatma Gandhi UniversityKottayam 686560KeralaIndia

Manganese-substituted magnetite ferrofluids(FFs)Mnx Fe_(1-x)Fe_(2)O_(4)(x=0–0.8)were prepared in this work through a chemical coprecipitation reaction.The controlled growth of FF nanomaterials for antibacterial activities is challenging,and therefore,very few reports are available on the topic.This research focuses on stabilizing aqueous FFs with the tetramethylammonium hydroxide surfactant to achieve high homogeneity.Morphological characterization reveals nanoparticles of 5–11 nm formed by the chemical reaction and nanocrystalline nature,as evident from structural investigations.Mn-substituted magnetic FFs are analyzed for their structural,functional,and antibacterial performance according to the Mn-substituent content.Optical studies show a high blue shift for Mn^(2+)-substituted Mnx Fe_(1-x)Fe_(2)O_(4)with the theoretical correlation of optical band gaps with the Mn content.The superparamagnetic nature of substituted FFs causes zero coercivity and remanence,which consequently influence the particle size,cation distribution,and spin canting.The structural and functional performance of the FFs is correlated with the antibacterial activity,finally demonstrating the highest inhibition zone formation for Mnx Fe_(1-x)Fe_(2)O_(4)FFs.

关键词： manganese ferrofluids homogeneity antibacterial stability

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Bagging with CTD–A Novel signature for the Hierarchical Prediction of secreted Protein Trafcking in Eukaryotes

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Genomics, Proteomics & Bioinformatics 2013年第6期11卷 385-390页

作者： Geetha Govindan Achuthsankar s.nair Department of Computational Biology and Bioinformatics University of Kerala

Protein trafficking or protein sorting in eukaryotes is a complicated process and is carried out based on the information contaified in the protein. Many methods reported prediction of the subcellular location of proteins from sequence information. However, most of these prediction methods use a flat structure or parallel architecture to perform prediction. In this work, we introduce ensemble classifiers with features that are extracted directly from full length protein sequences to predict locations in the protein-sorting pathway hierarchically. sequence driven features, sequence mapped features and sequence autocorrelation features were tested with ensemble learners and their performances were compared. When evaluated by independent data testing, ensemble based-bagging algorithms with sequence feature composition, transition and distribution （CTD） successfully classified two datasets with accuracies greater than 90%. We compared our results with similar published methods, and our method equally performed with the others at two levels in the secreted pathway. This study shows that the feature CTD extracted from protein sequences is effective in capturing biological features among compartments in secreted pathways.

关键词： sequence driven features sequence mapped features Autocorrelation Ensemble classifiter Protein sorting

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Regenerative Engineering for Knee Osteoarthritis Treatment： Biomaterials and Cell-Based Technologies

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Engineering 2017年第1期3卷 16-27页

作者： Jorge L. Escobar Ivirico Maumita Bhattacharjee Emmanuel Kuyinu lakshmi s. nair Cato T. Laurencin Institute for Regenerative Engineering University of Connecticut Health Center Farrnington CT 06030 USA Raymond and Beverly sackler Center for Biomedical Biological Physical and Engineering Sciences University of Connecticut Health Center Farmington CT 06030 USA Department of Orthopaedic surgery University of Connecticut Health Center Farmington CT 06030 USA Department of Biomedical Engineering School of Engineering University of Connecticut Storrs CT 06269 USA Department of Materials science and Engineering School of Engineering University of Connecticut Storrs CT 06269 USA Institute of Materials science University of Connecticut Storrs CT 06269 USA Department of Craniofacial sciences School of Dental Medicine University of Connecticut Health Center Farmington CF 06030 USA Department of Chemical and Biomolecular Engineering School of Engineering University of Connecticut Storrs CT 06269 USA

膝关节骨性关节炎(OA)是世界上最常见的关节炎,其发病率逐年上升。不断上涨的治疗费用给患者带来了经济负担。膝关节OA治疗的两个早期干预目标是减轻膝关节的疼痛和关节软骨的损害。目前用于治疗膝关节OA的方法虽取得了一定疗效,但仍没... 详细信息

膝关节骨性关节炎(OA)是世界上最常见的关节炎,其发病率逐年上升。不断上涨的治疗费用给患者带来了经济负担。膝关节OA治疗的两个早期干预目标是减轻膝关节的疼痛和关节软骨的损害。目前用于治疗膝关节OA的方法虽取得了一定疗效,但仍没有一种方法可达到全膝关节置换术(TKA)的治疗效果。TKA主要应用于治疗末期膝关节OA,其缺点是有手术侵入性和手术费用昂贵。因而,应该重视创新性的再生技术,以推迟甚至消除患者对TKA的需求。一些基于生物材料和细胞的疗法目前正处于发展阶段,并已在临床前和临床研究方面取得了初步进展。单独或联合应用先进的生物材料和干细胞治疗膝关节OA可减轻疼痛,使损害的关节软骨再生。在此综述中,我们讨论了膝关节OA疼痛和软骨损害的发病机制,并探讨了该病的最新治疗策略及其局限性。

关键词：膝关节骨性关节炎骨关节炎疼痛间充质干细胞生物材料再生工程

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Anti-fibrotic drugs dealing with pulmonary fibrosis after COVID-19-associated acute respiratory distress syndrome

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Journal of Acute Disease 2021年第4期10卷 177-178页

作者： Abhijit s.nair sai Kaushik Pulipaka Praveen Kumar Kodisharapu Asiel Christopher Department of Anaesthesiology Ibra HospitalNorth Sharqiya GovernorateIbra-414Sultanate of Oman Department of Anaesthesiology Basavatarakam Indo-American Cancer Hospital and Research InstituteHyderabad-500034Telangana StateIndia

severe acute respiratory syndrome coronavirus 2(sARs-CoV-2),the virus which is responsible for coronavirus disease(COVID-19),uses an angiotensin-2-converting enzyme(ACE2)as a cell receptor in humans.Initially,there is interstitial lung damage after infection,and then parenchymal lesions appear,which if not managed appropriately could worsen.Available data suggests that around 40%of patients with COVID-19 develop acute respiratory distress syndrome(ARDs),and 20%of these patients who develop ARDs require invasive and advanced modes of ventilation[1].

关键词： ACE2 drugs damage after

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Cellular interplay to 3D in vitro microphysiological disease model:cell patterning microbiota-gut-brain axis

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Bio-Design and Manufacturing 2024年第3期7卷 320-357页

作者： Kamare Alam lakshmi nair souvik Mukherjee Kulwinder Kaur Manjari singh santanu Kaity Velayutham Ravichandiran sugato Banerjee subhadeep Roy Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and ResearchKolkataWest Bengal 700054India Department of Pharmaceutical sciences Assam UniversitySilchar 788011India Department of Pharmaceutical sciences Guru Ghasidas UniversityKoniBilaspur 495009C.G.India school of Pharmacy and Biomolecular sciences RCSI University of Medicine and Health SciencesDublin D02 YN77Ireland Tissue Engineering Research Group Department of Anatomy and Regenerative MedicineRCSI University of Medicine and Health SciencesDublin D02 YN77Ireland Department of Pharmaceutics National Institute of Pharmaceutical Education and ResearchKolkataWest Bengal 700054India Department of Natural Products National Institute of Pharmaceutical Education and ResearchKolkataWest Bengal 700054India

The microbiota-gut-brain axis(MGBA)has emerged as a key prospect in the bidirectional communication between two major organ systems:the brain and the gut.Homeostasis between the two organ systems allows the body to function without disease,whereas dysbiosis has long-standing evidence of etiopathological conditions.The most common communication paths are the microbial release of metabolites,soluble neurotransmitters,and immune cells.However,each pathway is intertwined with a complex one.With the emergence of in vitro models and the popularity of three-dimensional(3D)cultures and Transwells,engineering has become easier for the scientific understanding of neurodegenerative diseases.This paper briefly retraces the possible communication pathways between the gut microbiome and the brain.It further elaborates on three major diseases:autism spectrum disorder,Parkinson’s disease,and Alzheimer’s disease,which are prevalent in children and the elderly.These diseases also decrease patients’quality of life.Hence,understanding them more deeply with respect to current advances in in vitro modeling is crucial for understanding the diseases.Remodeling of MGBA in the laboratory uses many molecular technologies and biomaterial advances.spheroids and organoids provide a more realistic picture of the cell and tissue structure than monolayers.Combining them with the Transwell system offers the advantage of compartmentalizing the two systems(apical and basal)while allowing physical and chemical cues between them.Cutting-edge technologies,such as bioprinting and microfluidic chips,might be the future of in vitro modeling,as they provide dynamicity.

关键词： Microbiota-gut-brain axis Neurodegeneration 3D disease model Organoid Transwell system

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structural,magnetic,and acidic properties of cobalt ferrite nanoparticles synthesised by wet chemical methods

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Journal of Advanced Ceramics 2015年第3期4卷 199-205页

作者： Manju KURIAN smitha THANKACHAN Divya s.nair Aswathy E.K. Aswathy BABU Arathy THOMAs Binu KRIsHNA K.T. Department of Chemistry Mar Athanasius CollegeKothamangalam-686666India Department of Physics Mar Athanasius CollegeKothamangalam-686666India

A detailed investigation on the effect of preparation method on the structural,magnetic,and acidic properties of cobalt ferrite nanoparticles prepared by sol-gel and co-precipitation is presented.Citric acid and ethylene glycol were used as gelling agents,while sodium hydroxide and aqueous ammonia were used as precipitating agents.The resulting ferrites were calcined at 450℃ and 750℃.sharper X-ray diffraction(XRD)peaks were observed for the samples calcined at 750℃,indicating greater crystallinity of the samples calcined at higher temperature.Average crystallite sizes fell in the ranges of 7.1-21.1 nm and 30.4-42.1 nm for the samples calcined at 450℃ and 750℃,respectively.The infrared spectra revealed two main absorption bands,the high frequency bandν1 around 600 cm^(-1) and the low frequency bandν2 around 400 cm^(-1) arising from stretching vibrations of the oxygen bond with the metal in the tetrahedral(A)and octahedral(B)sites in the spinel lattice.Agglomeration of particles was observed in the scanning electron microscopy(sEM)images.Magnetic parameters of CoFe_(2)O_(4) nanoparticles greatly depended on calcination temperature and preparation techniques.Ammonia temperature programmed desorption(TPD)measurements indicated that weak acid sites predominate medium strength sites,while the number of strong acid sites is the least.Cumulative acidity decreased for the samples calcined at higher temperature.The results underline the effect of preparation conditions on the morphology,crystallite size,and magnetic properties of nano ferrites.

关键词： nano ferrites sol-gel technique co-precipitation magnetic property surface acidity

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患有动静脉畸形的儿童和青年纳米氧化铁增强MRI后脑铁评估：病例对照研究

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国际医学放射学杂志 2021年第1期44卷 108-108页

作者： M.Iv N.N.Ng s.nair Y.Zhang J.Lavezo s.H.Cheshier 李佳佳

摘要氧化铁纳米粒子是MR成像的替代对比剂,钆沉积引起了安全问题,但目前尚不清楚纳米氧化铁是否也会沉积在脑组织中。目的研究儿童和青年纳米氧化铁增强后多

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