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Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry

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Genomics, proteomics & Bioinformatics 2021年第2期19卷 243-252页

作者： jozsef A.Balog Viktor Honti Eva Kurucz Beata Kari Laszlo G.puskas Istvan Ando Gabor j.Szebeni Laboratory of Functional Genomics Institute of GeneticsBiological Research CentreSzeged H-6726Hungary University of Szeged Ph.D.School in BiologySzeged H-6726Hungary Immunology Unit Institute of GeneticsBiological Research CentreSzeged H-6726Hungary Department of physiology Anatomy and NeuroscienceFaculty of Science and InformaticsUniversity of SzegedSzeged H-6726Hungary

Single-cell mass cytometry(SCMC)combines features of traditional flow cytometry(i.e.,fluorescence-activated cell sorting)with mass spectrometry,making it possible to measure several parameters at the single-cell level for a complex analysis of biological regulatory *** this study,we optimized SCMC to analyze hemocytes of the Drosophila innate immune *** used metal-conjugated antibodies(against cell surface antigens H2,H3,H18,L1,L4,and p1,and intracellular antigens 3A5 and L2)and anti-IgM(against cell surface antigen L6)to detect the levels of antigens,while anti-GFp was used to detect crystal cells in the immune-induced *** investigated the antigen expression profile of single cells and hemocyte populations in naive states,in immune-induced states,in tumorous mutants bearing a driver mutation in the Drosophila homologue of janus kinase(hopTum)and carrying a deficiency of the tumor suppressor gene lethal(3)malignant blood neoplasm-1[l(3)mbn1],as well as in stem cell maintenance-defective hdcD84 mutant *** analysis enabled the discrimination of the functionally different major hemocyte subsets for lamellocytes,plasmatocytes,and crystal cells,and delineated the unique immunophenotype of Drosophila *** have identified subpopulations of L2^(+)/p1^(+)and L2^(+)/L4^(+)/p1^(+)transitional phenotype cells in the tumorous strains l(3)mbn1 and hopTum,respectively,and a subpopulation of L4^(+)/p1^(+)cells upon immune *** results demonstrated for the first time that SCMC,combined with multidimensional bioinformatic analysis,represents a versatile and powerful tool to deeply analyze the regulation of cell-mediated immunity of Drosophila.

关键词： Mass cytometry Innate immunity Drosophila Single-cell analysis Hemocyte

NUMERICAL SIMULATION OF STRESS-STRAIN DISTRIBUTIONS FOR WELD METAL SOLIDIFICATION CRACKING IN STAINLESS STEEL

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Acta Metallurgica Sinica(English Letters) 2000年第1期13卷 133-138页

作者： Y. H. Wei R. p. Liu and Z. j. Dong( 1) National Key Laboratory of Advanced Welding production Technology, HIT, Harbin 150001, China 2) Harbin Research institute of Welding,Harbin 150080, China)

This paper analyzed the characteristics of welding solidification crack of stainless steels,and clearly re- vealed the the of the deformation in the molten - the pool and the solidification shrinkage on the stress - strain fields in the trail of molten - weld ***, rheologic properties of the alloys in solid - liquid zone were also obtained by measuring the hading and unloading deform curves of the *** a result, a numerical model for simulation of stress - strain distributions of welding solidifi- cation crack was developed. On the basis of the model,the thesis simulated the driving force of solidifi- cation crack of stainless steels, that is, stress - strain fields in the trail of molten-weld pool with fi- nite element method.

关键词： welding solidification crack welding solidification crack driving force,finite element method stress - strain field,numerical simulation

Characterization of Flow Structures Induced by Highly Rough Surface Using particle Image Velocimetry, proper Orthogonal Decomposition and Velocity Correlations

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Engineering（科研） 2018年第7期10卷 399-416页

作者： L.RAndersson I.A.S.Larsson j.G.I.Hellstrom p.Andreasson A.G.Andersson T.S.Lundstrom Division of Fluid and Experimental Mechanics Lulea University of TechnologyLuleaSweden Vattenfall Research and Development AlvkarlebySweden

High Reynolds number flow inside a channel of rectangular cross section is examined using particle Image Velocimetry. One wall of the channel has been replaced with a surface of a roughness representative to that of real hydropower tunnels, i.e. a random terrain with roughness dimensions typically in the range of ≈10% - 20% of the channels hydraulic radius. The rest of the channel walls can be considered smooth. The rough surface was captured from an existing blasted rock tunnel using high resolution laser scanning and scaled to 1:10. For quantification of the size of the largest flow structures, integral length scales are derived from the auto-correlation functions of the temporally averaged velocity. Additionally, proper Orthogonal Decomposition (pOD) and higher-order statistics are applied to the instantaneous snapshots of the velocity fluctuations. The results show a high spatial heterogeneity of the velocity and other flow characteristics in vicinity of the rough surface, putting outer similarity treatment into jeopardy. Roughness effects are not confined to the vicinity of the rough surface but can be seen in the outer flow throughout the channel, indicating a different behavior than postulated by Townsend’s similarity hypothesis. The effects on the flow structures vary depending on the shape and size of the roughness elements leading to a high spatial dependence of the flow above the rough surface. Hence, any spatial averaging, e.g. assuming a characteristic sand grain roughness factor, for determining local flow parameters becomes less applicable in this case.

关键词： CFD Validation Hydraulic Roughness pIV Hydropower

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磷盐岩研究进展

地球与环境 1984年第4期 12-12,37+63页

作者： p.j.Cook j.H.Shergold 刘艳平长沙二三○所

在过去的20年中,世界磷盐岩的消耗量从每年的50百万吨增至每年的150百万吨,其中大部分是用于生产磷肥。虽然世界磷盐岩总的说来并不短缺,而且已探明矿石达数十亿吨,但其分布范围是不均匀的。某些国家,如摩洛哥、美国、突尼斯和多哥,是... 详细信息

在过去的20年中,世界磷盐岩的消耗量从每年的50百万吨增至每年的150百万吨,其中大部分是用于生产磷肥。虽然世界磷盐岩总的说来并不短缺,而且已探明矿石达数十亿吨,但其分布范围是不均匀的。某些国家,如摩洛哥、美国、突尼斯和多哥,是磷盐岩的主要出口国。然而,许多国家却不产磷盐岩,而且它们多数是急需增加粮食产量的国家。磷盐岩分布的不均匀,是世界许多地区农业发展受到限制的关键因素。因此,近年来,寻找新的磷盐岩(特别

关键词：盐岩不均匀磷灰岩化学岩

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流域综合管理分论坛小结

水利水电快报 2012年第1期33卷 12-13页

作者： j.p.罗伯法瑞拉唐湘茜葡萄牙国家土木工程实验室

中国水利部长江水利委员会、中欧流域管理项目办等单位与机构的50余名中外嘉宾和代表出席了第四届长江论坛"流域综合管理理论与实践"分论坛,与会嘉宾围绕分论坛设置的4个议题积极建言献策。从水利科学技术的角度,介绍总结了中国目前的... 详细信息

中国水利部长江水利委员会、中欧流域管理项目办等单位与机构的50余名中外嘉宾和代表出席了第四届长江论坛"流域综合管理理论与实践"分论坛,与会嘉宾围绕分论坛设置的4个议题积极建言献策。从水利科学技术的角度,介绍总结了中国目前的流域管理工作进展以及与其他国际组织的合作状况,强调对水资源发展与环境保护应给予同等关注。通过建立中欧水资源交流平台可以加强相互合作,交流经验,为中国治江事业的进一步发展提供有益的借鉴。

关键词：流域综合管理管理分析分析总结

吗替麦考酚酯在熊脱氧胆酸不敏感的原发性胆汁性肝硬化患者中的治疗作用

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世界核心医学期刊文摘（胃肠病学分册） 2005年第6期1卷 53-54页

作者： Talwalkar j.A. Angulo p. Keach j.C. 王铮 Div. of Gastroenterol. and Hepatol. Mayo Clinic and Foundation 200 First Street SW RochesterMN55905 United States Dr.

Background&Aims: Despite evidence for therapeutic efficacy with ursodeoxycholic acid (UDCA) in primary biliary cirrhosis (pBC), only 30-50%of patients achieve complete biochemical remission within 1 year of therapy. Mycophenolate mofetil (MMF) is an immunosuppressive medication that inhibits T and B lymphocyte proliferation. The aim of this investigation was to determine the safety and estimated efficacy of MMF in patients with pBC. Methods: Twenty-five patients with incomplete responses to UDCA (defined as persistent elevation of serum alkaline phosphatase ≥2 times the upper limit of normal) received MMF 1 g daily to a maximum of 3 g daily with UDCA (13-15 mg/kg per day) for 1 year. Liver biochemistries were determined at 3-month intervals with Mayo Risk Score calculated at baseline and end of therapy. Results: Nineteen (76%) patients completed 1 year of therapy. Despite improvements in serum alkaline phosphatase (920 ±308 vs. 709 ±242 IU/L, p = 0.001) and AST (65 ±31 vs. 51 ±19 IU/L, p = 0.007) levels, these findings were clinically insignificant. Exploratory analysis revealed a strong correlation between advanced pBC defined by higher Mayo Risk Score and reduction in serum alkaline phosphatase levels (r = -0.74, p = 0.006). Six patients (24%) did not complete therapy; adverse drug events were responsible for study withdrawal in 3 individuals. Adverse reactions that resolved spontaneously or by dose reduction occurred in 13 patients. Conclusions: MMF is not associated with important clinical benefits in pBC based on the results of this pilot investigation.

关键词：吗替麦考酚酯治疗作用熊脱氧胆酸正常值上限血清碱性磷酸酶免疫抑制剂中评估生化指标

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磁铅矿型和B—氧化铝型磷光体的能量传递

发光学报 1977年第2期 13-14页

作者： j.M.p.jVerstegen,j.L.Sommerdijk,石军岩爱恩德荷文菲利浦光学部研究实验室爱恩德荷文菲利浦光学部研究实验室

六边形的磁铅矿和和β-氧化铝是由隔开的尖晶石块组成的结构,在结构中每个垂直于C轴的、将尖晶石块隔开的中间层含有一个大的阳离子,分别含有三个和一个氧离子。用Mn2+、TI+Eu2+、Ce3+、Tb3+或这些离子的组合激活的具有磁铅矿的β一氧... 详细信息

六边形的磁铅矿和和β-氧化铝是由隔开的尖晶石块组成的结构,在结构中每个垂直于C轴的、将尖晶石块隔开的中间层含有一个大的阳离子,分别含有三个和一个氧离子。用Mn2+、TI+Eu2+、Ce3+、Tb3+或这些离子的组合激活的具有磁铅矿的β一氧化铝结构的镓酸盐和铝酸盐,是高效率的磷光体。从晶体结构考虑Tl+、Eu2+、Ce2+

关键词：能量传递磷光体电偶极子铅矿氧化铝铝化合物

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对一颗快速脉动射电源的观测

北京大学学报（自然科学版） 2002年第B12期38卷 66-72,132-135页

作者： A.休伊什 S.j.贝尔 j.D.H.皮尔金顿 p.F.司科特 R.A.科林斯徐仁新剑桥大学卡文迪什实验室缪拉德射电天文台

缪拉德射电天文台记录到了来自某种脉动射电源的不平常信号。这种辐射似乎起源于银河系内天体,可能与白矮星或中子星的震荡有关。在1967年7月,一台工作频率为81.5MHz的大型射电望远镜在缪拉德射电天文台交付使用。设计这一仪器是为了通... 详细信息

缪拉德射电天文台记录到了来自某种脉动射电源的不平常信号。这种辐射似乎起源于银河系内天体,可能与白矮星或中子星的震荡有关。在1967年7月,一台工作频率为81.5MHz的大型射电望远镜在缪拉德射电天文台交付使用。设计这一仪器是为了通过观测由行星际介质不均匀分布和不规则运动导致的闪烁1),来研究致密射电源的角结构[1]。开始巡天天区的赤纬δ在-08°和44°之间,该天区每周扫描一次。因而,这个常规的巡天覆盖了大部分天区。此设备工作不久就记录到一种信号,它们起先很弱且零星地出现,随后能够在固定的赤经和赤纬重复观测到。这些结果意味着,这种辐射起源于地外。系统的研究开始于当年11月。高速记录显示,出现的信号由一系列的脉冲组成;脉冲持续时间约0.3s,以大约1.337s为周期重复出现且维持非常精确的周期。进一步的观测表明,尽管存在因地球轨道运动导致观测周期的系统变化,真实周期的精度优于10-7。另外,被记录的周期性脉冲信号具有在接收机1MHz带宽内随频率递减的特征2)。起先认为这种信号的非凡特征起源于某种人为的传输过程,可能与深空探测器、行星雷达或与地面信号被月球的反射有关。然而,即使那些源位于太阳系的边缘也应该存在视差...

关键词：射电天文学天文观测辐射中子星白矮星快速脉动射电源

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CW环形染料激光器的无反射镜腔体

应用光学 1988年第4期 31-33+53页

作者： j.jasy j.Sepiol U.p.Wild 徐东

本文论述了CW环形染料激光器的新型无反射镜腔体结构。腔体是根据新型的布鲁斯特入射角和全内反射焦聚棱镜设计的。专用的屋脊棱镜可以调节波长。这些棱镜都是用无涂层石英制成的,因此,染料激光器工作的波长范围较大。该文报道了采用若... 详细信息

本文论述了CW环形染料激光器的新型无反射镜腔体结构。腔体是根据新型的布鲁斯特入射角和全内反射焦聚棱镜设计的。专用的屋脊棱镜可以调节波长。这些棱镜都是用无涂层石英制成的,因此,染料激光器工作的波长范围较大。该文报道了采用若丹明6G染料,使CW运转及腔内倍频的同步泵浦锁模运转。

关键词：染料激光器谐振腔液体激光器同步泵浦锁模腔体结构布鲁斯特角腔内倍频非线性晶体屋脊棱镜全内反射环形激光器聚焦装置反射镜反光镜 CW