The discovery and application of genome editing introduce a new era of plant breeding,giving researchers efficient tools for the precise engineering of crop ***, we demonstrate the power of genome editing for engineer...
The discovery and application of genome editing introduce a new era of plant breeding,giving researchers efficient tools for the precise engineering of crop ***, we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice( Oryza sativa).We first isolated a lesion mimic mutant( LMM) from a mutagenized rice population, demonstrated that a29-bp deletion in a gene we named RESISTANCE TO BLAST1( RBL1) caused this phenotype and showed that this mutation caused a ca.20-fold reduction in *** encodes a cytidine diphosphate diacylglycerol(CDP-DAG) synthase required for phospholipid *** of RBL1 results in reduced levels of phosphatidylinositol(P1) and its derivative PI(4, 5) *** PI(4, 5) P is enriched in cellular structures specifically associated with effector secretion and fungal infection, suggesting a role as a disease susceptibility *** targeted genome editing, we obtained an allele of RBL1, named RBL1, which confers broad-spectrum resistance but does not decrease yield in a model rice variety as assessed in small-scale field *** study has demonstrated the usefulness of editing of an LMM gene, a strategy relevant to diverse LMM genes and crops.
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