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Charcot-Marie-Tooth-1A and sciatic nerve crush rat models:insights from proteomics

Neural Regeneration Research 2023年第6期18卷 1354-1363页

作者： Zeina Msheik Stephanie Durand Emilie Pinault Martial Caillaud laetitia vignaud Fabrice Billet Mohamed El Massry Alexis Desmoulière UR20218 NeurIT(NEURopathies périphériques et Innovation Thérapeutique) University of LimogesLimogesFrance BISCEm(Biologie Intégrative SantéChimie Environnement)Platform US 42 Inserm/UAR 2015 CNRSUniversity of LimogesLimogesFrance UMR 1308 Inserm/CHU–CAPTuR(Contrôle de l’Activation cellulaire Progression Tumorale et Résistance thérapeutique)University of LimogesLimogesFrance Inserm UMR1235–TENS(The Enteric Nervous System in Gut and Brain Diseases) University of NantesNantesFrance

The sensorimotor and histological aspects of peripheral neuropathies were already studied by our team in two rat models:the sciatic nerve crush and the Charcot-Marie-Tooth-1A *** this study,we sought to highlight and compare the protein signature of these two pathological ***,the identification of protein profiles in diseases can play an important role in the development of pharmacological *** fact,Charcot-Marie-Tooth-1A rats develop motor impairments that are more severe in the hind ***,for the first time,protein expression in sciatic nerve of Charcot-Marie-Tooth-1A rats was ***,distal sciatic nerves were collected from Charcot-Marie-Tooth-1A and uninjured wild-type rats aged 3 *** protein extraction,sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass spectrometry was employed.445 proteins mapped to Swiss-Prot or trEMBL Uniprot databases were identified and *** these,153 proteins showed statistically significant differences between Charcot-Marie-Tooth-1A and wild-type *** majority of these proteins were overexpressed in *** clustering and functional enrichment using Gene Ontology were used to group these proteins based on their biological effects concerning Charcot-Marie-Tooth-1A ***,proteomic characterization of wild-type rats subjected to sciatic nerve crush was performed sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass *** month after injury,distal sciatic nerves were collected and analyzed as described *** of 459 identified proteins,92 showed significant differences between sciatic nerve crush and the uninjured wild-type rats used in the first *** results suggest that young adult Charcot-Marie-Tooth-1A rats(3 months old)develop compensatory mechanisms at the level of redox balance,protein folding,myelination,and

关键词： Charcot-Marie-Tooth-1A endoplasmic reticulum Gene Ontology neurogenesis oxidative stress proteomics rat repair sciatic nerve crush SWATH-MS

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The interactive effect of temperature and fertilizer types determines the dominant microbes in nitrous oxide emissions and the dicyandiamide efﬁcacy in a vegetable soil

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Soil Ecology Letters 2024年第3期6卷 121-133页

作者： Xiaoya Xu Haiyang Liu Yaowei Liu Didier Lesueur laetitia Herrmann Hongjie Di Caixian Tang Jianming Xu Yong Li Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China College of Geography and Environment Shandong Normal UniversityJinan 250014China Centre de Coopération Internationale en Recherche Agronomique pour le Développement(CIRAD) UMR Eco&SolsHanoiVietnam Eco&Sols Universitéde Montpellier(UMR)CIRADInstitut National de Recherche pour l’Agriculturel’Alimentation et l’Environnement(INRAE)Institut de Recherche pour le Développement(IRD)Montpellier SupAgro34060 MontpellierFrance Alliance of Biodiversity International and International Center for Tropical Agriculture(CIAT) Asia hubCommon Microbial Biotechnology Platform(CMBP)HanoiVietnam School of Life and Environmental Sciences Faculty of ScienceEngineering and Built Environment–Deakin UniversityMelbourneVIC 3125Australia Rubber Research Institute Chinese Academy of Tropical Agricultural SciencesHaikou 571737China La Trobe Institute for Sustainable Agriculture and Food Department of AnimalPlant&Soil SciencesLa Trobe UniversityBundooraVic 3086Australia

Heat waves associated with global warming and extreme climates would arouse serious consequences on nitrogen(N)***,the responses of the functional guilds to different temperatures,especially high temperature and the cascading effect on N_(2)O emissions remain *** incubation study was conducted to examine the effect of different temperatures(20°C,30°C,and 40°C)and fertilizer types(urea and manure)on N_(2)O-producers and N_(2)O-reducers,as well as the efficacy of dicyandiamide(DCD)on N_(2)O emissions in a vegetable *** showed that ammonia oxidizers and nirS-type denitrifiers were well adapted to high temperature(40°C)with manure application,while the fungal nirK-denitrifiers had better tolerance with urea *** nosZ clade I microbes had a strong adaptability to various temperatures regardless of fertilization type,while the growth of nosZ clade II group microbes in non-fertilized soil(control)were significantly inhibited at higher *** N_(2)O emissions were significantly decreased with increasing temperature and DCD application(up to 60%,even at 40°C).Under high temperature conditions,fungal denitrifiers play a significant role in N-limited soils(non-fertilized)while nirS-type denitrifiers was more important in fertilized soils in N_(2)O emissions,which should be specially targeted when mitigating N_(2)O emissions under global warming climate.

关键词： nitrogen fertilizer microorganisms nitrification inhibitor bacteria archaea fungi

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Osgood Schlatter Disease: Study of 2 Observations in Conakry, Guinea

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Open Journal of Rheumatology and Autoimmune Diseases 2024年第2期14卷 77-83页

作者： Adama Bah laetitia Cynthia Abandazegoue Andjembe Abdoulaye Barry Mamadou Lamine Diallo Moustapha Niasse Aly Badra Kamissoko Rheumatology Department Ignace Deen Hospital Conakry Conakry Guin&#233e Rheumatology Department CHU Aristide Le Dantec of Dakar Dakar S&#233n&#233gal Department of Rhumatology Sylvanus Olypio University Hospital Lom&#233 Lom&#233 Togo

Introduction: OSD is a growth osteochondrosis affecting the apophyseal cartilage of the anterior tibial tuberosity of the knee, 20 to 30% of young growing athletes suffer from this pathology, described by Osgood and Schlatter in 1903. Objective: To report 2 observations and review the literature. Observation 1: This was a 14-year-old Guinean teenager, Halpoular, living in Conakry, a right-handed footballer who presented with a painful swelling of the right knee that looked mechanical, whose father had HLA B27 positive ankylosing spondylitis. X-ray of the right knee showed fragmentation of the anterior tibial tuberosities, and ossicle with edema of the patellar tendons at stage 4 according to Ehrenbord and Lagergren (1961). Thus, the disease of OSD is retained, He received 120 mg of diclofenac, 20 mg omeprazole for 1 month and the cessation of sports activity, the evolution was favorably marked by the regression of pain. Observation 2: A 16-year-old girl, initials M.L.T, Guinean, residing in Coyah (Republic of Guinea), a handball player, consulted for intense pain (VAS: 8/10) in her knees after a violent shock received when she hit the post. The physical examination found a painful, soft swelling at the anterior tibial tuberosity of the right knee, the rest of the examination was normal. The X-ray of the right knee shows fragmentation of the anterior tibial tuberosity, and ossicle with edema of the patellar tendon at stage 4 according to Ehrenbord and Lagergren 1961, thus the OSD disease is retained, the treatment instituted was 120 mg of diclofenac, the cessation of sports activity which gives a good evolution (EVA at 2/10). Conclusion: OSD is a growth osteochondrosis affecting the apophyseal cartilage of the anterior tibial tuberosity of the knee in young growing athletes, resolving at rest.

关键词： Osgood Shlatter Disease Young Athlete

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Epidemiological, Clinical Progress Aspects of Congenital Heart Disease with Neonatal Revelation at the Mother-Child Hospital of Bingerville (HME) Concerning 98 Cases from January 2021 to December 2022 (Côte d’Ivoire)

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Open Journal of Pediatrics 2024年第1期14卷 89-100页

作者： Adja Evelyne Akaffou-Gbery Richard Azagoh-Kouadio Anne-Lise laetitia Mobio Jean-Jacques Yao Atteby Serenah Marie Janice N’vrah Anoh Kouassi Raoul Yao Department of Paediatrics and Neonatology H&#244pital Mère-Enfant de Bingerville (HME) Bingerville C&#244te d’Ivoire Department of Medical Pediatrics and Specialties CHU de Angré Abidjan C&#244te d’Ivoire

Introduction The distribution of congenital heart disease (CHD) in sub-Saharan Africa is highly imprecise and varies from one region to another due to the inequality of diagnostic facilities. The aim of this study was to determine the in-hospital prevalence of congenital heart disease in children at the Mother-Child hospital of Bingerville (HME) by specifying the diagnostic, therapeutic and evolutionary aspects. Materials and methods We conducted a retrospective, descriptive, cross-sectional study at HME of Bingerville from January 2021 to December 2022. All newborns with congenital heart disease confirmed by echocardiography were included in the study. Results Of 656 admissions to the neonatology department over the study period, congenital heart disease accounted for 14.9% (98/656) of cases. In our series, 76.7% were diagnosed before the 1st week of life, with a mean chronological age of 5.18 days and extremes of 0 and 46 days. There were as many male patients (50%) as female (50%), i.e. a sex ratio of 1. These newborns were premature in 60.2% of cases, with a mean and median gestational age of 34 weeks’ amenorrhea. Most were left-right shunts (90.8%). Persistent ductus arteriosus (PDA) (48.9%) predominated, followed by atrial septal defect (38.7%), ventricular septal defect (13.3%), common trunk artery (CTA) (3.1%) and open septal pulmonary atresia (OSPA) (1%) as the primary cyanogenic heart disease. Pulmonary arterial hypertension (PAH) (50%) was primary in 38.8% and secondary (61.2%). The mortality rate was 30.6%, and all CTA patients died (100%), with a significant statistical relationship (p = 0.027). Progression under treatment was marked by clinical stabilization (68/98) in 69.4% of cases. Conclusion: Congenital heart disease is relatively common at the Bingerville HME. Access to echocardiography should be facilitated in neonatology departments for rapid diagnosis and optimal management of congenital heart disease in newborns.

关键词： Newborn Congenital Heart Disease Côte d’Ivoire

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Nanoparticles-based nanochannels assembled on a plastic flexible substrate for label-free immunosensing

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Nano Research 2015年第4期8卷 1180-1188页

作者： Alfredo de la Escosura-Muniz Marisol Espinoza-Castaneda Madoka Hasegawa laetitia Philippe Arben Merkoci ICN2-Nanobioelectronics & Biosensors Group Institut Catala de Nanociencia i Nanotecnologia Campus UAB 08193 Bellaterra (Barcelona) Spain EMPA Swiss Federal Laboratories for Materials Science and Technology Feuerwerkerstrasse 39 3602Thun Switzerland ICREA-Institucio Catalana de Recerca i Esrudis Avan@ats 08010 Barcelona Spain

A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres （PS, 200 and 500 nm-sized） onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate （ITO/PET） electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe（CN）6]^4- flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G （IgG）（chosen as a model analyte） to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.

关键词： nanochannel dip-coating.electrochemical biosensor label-free immunosensing

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Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants

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Molecular Plant 2019年第1期12卷 99-112页

作者： Allen Yi-Lun Tsai Takumi Higaki Chinh-Nghia Nguyen laetitia Perfus-Barbeoch Bruno Favery Shinichiro Sawa Graduate School of Science &Technology Kumamoto University2-39-1 KurokamiChuo-kuKumamoto 860-8555Japan Graduate School of Frontier Sciences The University of Tokyo5-1-5 KashiwanohaKashtwa-shiChiba 277-8562Japan INRA Universite Cote d'AzurCNRSUMR 1355-7254 Institut Sophia Agrobiotech06900 Sophia AntipolJsFrance present address:Internattonal Research Organization for Advanced Science &Technology Kumamoto University2-39-1 KurokamiChuo-kuKumamoto 860-8555Japan Present address:School of Biotechnology and Food Technology Hanoi University of Science and Technology10 000 HanoiVietnam

Seed exudates influence the behavior of soil organisms,but howthis occurs remains unclear,particularly for multicellular *** we show that compounds associated with Arabidopsis seed-coat mucilage regulate the behavior of soil-borne animals,specifically root-knot nematodes (RKNs).Infective RKN J2 larvae actively travel toward Arabidopsis seeds through *** of Arabidopsis mucilage mutants demonstrated that the attraction of RKNs toArabidopsis seeds requires the synthesis and extrusion ***-coat *** mucilage alone is not sufficient to attract RKNs,but seed-surface carbohydrates and proteins are required for this *** findings suggest that the RKN chemoattractant is synthesized de novo upon mucilage extrusion but may be highly *** attracted by thismucilage-dependent mechanism can infect the emerging ***,the attraction signal from seedling roots likely acts independently of the seed-coat signal and may mask the attraction to seed-coat mucilage after *** RKN species are attracted byArabidopsis seeds,suggesting that this mechanism is conserved in *** findings indicate that seed exudate can regulate the behavior of multicellular animals and highlight the potential roles of seed-coat mucilage in biotic interactions with soil microorganisms.

关键词： chemotaxis seed-coat mucilage root-knot nematode plant-pathogen interaction

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Methodological Approaches to Study Internet Financial Communication

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Journal of Modern Accounting and Auditing 2015年第1期11卷 27-40页

作者： Pozniak laetitia University ofMons Mons Belgium

The aim of this paper is to show how qualitative and quantitative approaches can be complementary to study internet financial communication in a thesis by papers and how grounded theory （GT） can be the link among the different papers of the thesis. The study context of our thesis was the unregulated markets of New York Stock Exchange （NYSE） Euronext Brussels and the problematic rose from this context： What is the voluntary effort of communication when there is no obligation of internet financial communication？ Four papers tried to answer this central question and other following research questions. To answer those research questions, several methodological approaches were used： content analysis of websites and scoring, linear regression, paired sample, and interviews. At the end of our thesis by papers, we discovered that GT was the general methodological travel among the papers： Every article had for vocation to try to answer the questions raised by the previous article.

关键词： quantitative and qualitative approaches grounded theory (GT) induction financial communication internet unregulated markets

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Self-activation of Vγ9Vδ2 T cells by exogenous phosphoantigens involves TCR and butyrophilins

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Cellular & Molecular Immunology 2021年第8期18卷 1861-1870页

作者： ChloéLaplagne laetitia Ligat Juliet Foote Frederic Lopez Jean-Jacques Fournié Camille Laurent Salvatore Valitutti Mary Poupot Inserm UMR1037 Centre de Recherches en Cancérologie de ToulouseToulouseFrance UniversitéToulouse III Paul-Sabatier ToulouseFrance ERL 5294 CNRS ToulouseFrance IUCT-O ToulouseFrance

The high cytotoxic activity of Vγ9Vδ2 T lymphocytes against tumor cells makes them useful candidates in anticancer ***,the molecular mechanism of their activation by phosphoantigens(PAgs)is not completely *** studies have depicted the mechanism of Vγ9Vδ2 T-cell activation by PAg-sensed accessory cells,such as immune presenting cells or tumor *** this study,we demonstrated that pure resting Vγ9Vδ2 T lymphocytes can self-activate through exogenous PAgs,involving their TCR and the butyrophilins BTN3A1 and *** is the first time that these three molecules,concurrently expressed at the plasma membrane of Vγ9Vδ2 T cells,have been shown to be involved together on the same and unique T cell during PAg ***,the use of probucol to stimulate the inhibition of this self-activation prompted us to propose that ABCA-1 could be implicated in the transfer of exogenous PAgs inside Vγ9Vδ2 T cells before activating them through membrane clusters formed byγ9TCR,BTN3A1 and *** self-activation of Vγ9Vδ2 T cells,which leads to self-killing,can therefore participate in the failure ofγδT cell-based therapies with exogenous PAgs and should be taken into account.

关键词： Vγ9Vδ2 T cells Phosphoantigen Butyrophilins T-cell receptor

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Effect of low temperature in the development cycle of Lucilia sericata (Meigen) (Diptera, Calliphoridae): implications for the minimum postmortem interval estimation

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Forensic Sciences Research 2018年第1期3卷 52-59页

作者： laetitia Cervantès Laurent Dourel Emmanuel Gaudry Thierry Pasquerault Benoit Vincent Forensic Fauna Flora Department Institut de Recherche Criminelle De La Gendarmerie nationaleCergy Pontoise CedexFrance

Knowledge of necrophagous insects' developmental data is necessary for the forensic entomologist to estimate a reliable minimum postmortem interval (PMImin).Among the most represented necrophagous species,Lucilia sericata (Diptera,Calliphoridae) is particularly *** is regularly identified in samples,with a predominance in summer,and is commonly used by analysts of our entomology department (Institut de Recherche Criminelle de la Gendarmerie Nationale) to estimate the PMImin with the accumulated degree days (ADD) *** method requires the mathematical lower thermal threshold to be *** value dictates the quality of the applied ADD method but cannot be considered as fixed,especially when insect development occurs at temperatures close to the biological *** such conditions,it is necessary to study the influence of such temperatures on development rate,as well as the consequences of estimating the period of first oviposition on cadavers,when using the ADD *** replicate rearings were conducted at six different temperatures: 30 ℃,24 ℃,18 ℃,15 ℃,12 ℃ and 10 ℃.Time of development and time of emergence were *** effect of low temperature on the development cycle and the reliability of the ADD method under this entire temperature spectrum were studied using different linear regression *** durations of total insect time development and experimental rearing duration were then compared.A global linear model cannot be used on the whole temperature spectrum experienced by *** without resulting in an overestimation at some *** found a combination of two linear regression models to be suitable for the estimation of the total development time,depending on the temperature experienced by *** approach allowed us to obtain a variation lower than 2% at 12 ℃ and 10 ℃ between the calculated duration and experimental duration of *** comparison,the results obtained with a global model show

关键词： Forensic science forensic entomology accumulated degree days(ADD)method minimum PMI low temperature

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NTR/NRX Define a New Thioredoxin System in the Nucleus of Arabidopsis thaliana Cells

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Molecular Plant 2014年第1期7卷 30-44页

作者： Corinne Marchal Valerie Delorme-Hinoux laetitia Bariat Wafi Siala Christophe Belin Julio Saez-Vasquez Christophe Riondet Jean-Philippe Reichheld Universite Perpignan Via Domitia CNRS Laboratoire Genome et Developpement des Plantes UMR 5096 58 Avenue Paul Alduy-Bat T F-66860 Perpignan France Pharmacologie cellulaire et moleculaire Louvain Drug Research Institute Universite catholique de Louvain Brussels Belgium

Thioredoxins （TRX） are key components of cellular redox balance, regulating many target proteins through thiol/disulfide exchange reactions. In higher plants, TRX constitute a complex multigenic family whose members have been found in almost all cellular compartments. Although chloroplastic and cytosolic TRX systems have been largely studied, the presence of a nuclear TRX system has been elusive for a long time. Nucleoredoxins （NRX） are potential nuclear TRX found in most eukaryotic organisms. In contrast to mammals, which harbor a unique NRX, angiosperms gen- erally possess multiple NRX organized in three subfamilies. Here, we show thatArabidopsis thaliana has two NRXgenes （AtNRX1 and AtNRX2）, respectively, belonging to subgroups I and III. While NRX1 harbors typical TRX active sites （WCG/ PPC）, NRX2 has atypical active sites （WCRPC and WCPPF）. Nevertheless, both NRXl and NRX2 have disulfide reduction capacities, although NRX1 alone can be reduced by the thioredoxin reductase NTRA. We also show that both NRX1 and NRX2 have a dual nuclear/cytosolic localization. Interestingly, we found that NTRA, previously identified as a cytosolic protein, is also partially localized in the nucleus, suggesting that a complete TRX system is functional in the nucleus. We show that NRX1 is mainly found as a dimer in vivo. nrxl and nrx2 knockout mutant plants exhibit no phenotypic perturbations under standard growth conditions. However, the nrxl mutant shows a reduced pollen fertility phenotype, suggesting a specific role of NRX1 at the haploid phase.

关键词： thioredoxin nucleoredoxin nucleus Arabidopsis.

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