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电视胸腔镜手术与开胸手术治疗肺大疱的疗效对比

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检验医学与临床 2015年第6期12卷 727-728,731页

作者：曾小飞尚观胜马瑞东郭利明成都医学院第一附属医院成都610500

目的对比分析电视胸腔镜手术与开胸手术治疗肺大疱的疗效。方法选取2013年1月至2014年5月于成都医学院第一附属医院心胸外科住院治疗的肺大疱患者47例,按照手术方式不同分为电视胸腔镜手术治疗组(34例)和常规开胸手术治疗组(13例),比较... 详细信息

目的对比分析电视胸腔镜手术与开胸手术治疗肺大疱的疗效。方法选取2013年1月至2014年5月于成都医学院第一附属医院心胸外科住院治疗的肺大疱患者47例,按照手术方式不同分为电视胸腔镜手术治疗组(34例)和常规开胸手术治疗组(13例),比较两组各项临床观察指标及手术疗效。结果两组手术时间比较差异无统计学意义(P>0.05);与常规开胸手术治疗组比较,电视胸腔镜手术治疗组患者术中出血量与术后引流量减少,疼痛时间与住院时间缩短,但住院费用增加,组间比较差异均有统计学意义(P<0.05);且电视胸腔镜手术治疗组总有效率(97.0%)高于开胸手术治疗组(76.9%),组间比较差异有统计学意义(P<0.05)。结论电视胸腔镜手术治疗肺大疱的疗效优于常规开胸手术,有利于患者术后恢复,值得临床推广应用。

关键词：肺大疱电视胸腔镜手术开胸手术

Orientation growth of N-doped and iron-based metal organic frameworks based on the usage amount of lewis base and its application for the preconcentration of allergenic disperse dyes

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Orientation growth of N-doped and iron-based metal organic f...

第十一届全国化学生物学学术会议

作者： Ming-li Ye Yin Lu zhu Yan Mei-Lan Chen College of Biological and Environmental Engineering Zhejiang Shuren University zhejiang University

Here,we report the orientation growth and highly ordered N-doped and iron-based metal organic frameworks（N-d-Fe-MOFs） through a simple and low-cost self-assembly process using solvothermal *** effect of the usage amount of lewis base on the orientation growth of N-d-Fe-MOFs has been investigated and *** increasing of the usage amount of lewis base,i.e.,ammonium hydroxide,the morphological of N-d-Fe-MOFs changed correspondingly as shown in *** can be seen that the N-d-Fe-MOFs were spheroidal without using ammonium hydroxide during preparation ***,they gradually changed to be cubic and pyramidal with using 1.0 m L and 2.0 m L of ammonium hydroxide during preparation process,***,the excellent N-d-Fe-MOFs,which are used as sorbent in magnetic dispersive solid phase extraction（Mag-dSPE） procedure,has been applied for the extraction of allergenic disperse dyes from environmental water,and the effect of several factors,i.e.,pH value of samples,adsorption time and temperature,elution solvent,desorption time and temperature,salt effect and coexisting ion effect on the preconcentration efficiency of N-d-Fe-MOFs have been investigated *** obtained results show the higher extraction capacity of N-d-Fe-MOFs with recoveries in the range of 83.0%-116%.Moreover,the N-d-Fe-MOFs Mag-dSPE method coupled with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry（UFLC-MS/MS） has been developed for the fast determination of allergenic disperse dyes in river at sub-ppt *** limits of quantification（LOQs） for allergenic disperse dyes,i.e.,disperse yellow 1,disperse red 1,disperse blue 1,disperse brown 1 and disperse orange 1,are between 0.52-12.06 ng/*** results on linearity,accuracy and precision,as well as on application to the analysis of allergenic disperse dyes in twenty real samples demonstrate the applicability to environment monitoring analysis.

关键词： Lewis base N-doped and iron-based metal organic frameworks (N-d-Fe-MOFs) Allergenic disperse dyes Magnetic dispersive solid phase extraction(Mag-dSPE) UFLC–MS/MS

胆汁三烯结合蛋白在大肠杆菌中的表达、复性与纯化

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生物技术通讯 2009年第2期20卷 191-194页

作者：李晓芳宁保安胡红芳杨丽丽左佳王彩红高志贤华中农业大学食品科技学院湖北武汉430070 军事医学科学院卫生学环境医学研究所天津300050

目的:合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达,获得重组BBP纯化制品。方法:根据天然BBP的基因序列和大肠杆菌偏好密码子设计并合成BBP基因的引物,PCR扩增优化的BBP基因序列,克隆至载体pEasy-T3;测序正确后,将该序列克隆至表... 详细信息

目的:合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达,获得重组BBP纯化制品。方法:根据天然BBP的基因序列和大肠杆菌偏好密码子设计并合成BBP基因的引物,PCR扩增优化的BBP基因序列,克隆至载体pEasy-T3;测序正确后,将该序列克隆至表达载体pET-32a上,构建表达质粒,转化至大肠杆菌BL21(DE3)pLysS,在IPTG诱导下表达融合蛋白;采用Ni柱纯化融合蛋白。结果:PCR扩增获得了优化后的BBP基因序列,构建了表达载体pET-32a-BBP;SDS-PAGE分析表明表达的融合蛋白相对分子质量为20×103,以包涵体形式存在,占全菌蛋白的40%以上;变性、复性后经Ni2+柱纯化,获得纯度达98%以上的重组蛋白。结论:优化并合成了BBP全基因序列,获得了高纯度重组融合蛋白,为进一步鉴定其生物活性及筛选小分子的研究奠定了基础。

关键词：胆汁三烯结合蛋白大肠杆菌融合蛋白

Establishment of a humanized mouse model with the reconstitution of human immune system and engraftment of autologous human hepatocytes

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Establishment of a humanized mouse model with the reconstitu...

第十一届全国免疫学学术大会

作者： Guo Jing-long li yang Hu zh.ng Yang Yong-guang The First Bethune Hospital and Institute of Immunology Jilin University

Background and Aim: Infections caused by hepatotropic pathogens, such as HBV, still raise concerns worldwidely while with no effective curable regimens. Lack of suitable animal model to directly study the immunopathogenesis of human hepatotropic viral infections is a main bottleneck for novel drug investigations. Thus, establishment of a versatile mouse model harboring both functional human immune system and human hepatocytes would be of high ***: To achieve human hepatocyte repopulation in mouse liver, enriched human fetal liver progenitors were injected into the spleen of immunodeficient mice followed with continuous treatment of anti-mouse fas antibody. Humanized mice with functional human immune system were generated by co-transplantation of human fetal thymic tissues(under renal capsules) and human fetal liver CD34+ cells(intravenous injection) into immunodeficient animals after sublethal total body ***: Secretion of human albumin in the animals transplanted with human fetal liver progenitor cells lasted over 14 weeks post transplantation, and reached up about to 400ng/m L. Immunohistochemical staining of mouse liver section by anti-human hepatocyte(Hep par 1), anti-human CK8/18, and anti-human albumin antibodies revealed that around 30-40% human hepatocytes repopulated in the humanized mouse livers. Moreover, the chimerism of human immune cells in the periphery blood and livers of the humanized mice were approximately 80% and 90% respectively at 14 weeks posttransplantation of Thy/CD34+ cells, including CD3+(both CD8 and CD8) T cells, CD19+ B cells, as well as CD14+ monocytes/macrophages and so ***: We developed a humanized mouse model with high level of human immune system and autologous human hepatocyte engraftment which may serve as a valuable model to study human hepatotropic pathogen infection.

关键词： Humanized mice NOD/SCID Hepatocyte engraftment Immune reconstitution

The association of serum vitamin D and calcium levels with the occurrence and development of uterine fibroids

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The association of serum vitamin D and calcium levels with t...

2018年浙江省妇科肿瘤学学术年会暨温台妇产科年会

作者： Saisai li Beilei Chen Bo Sheng Jing Wang Xueqiong zhu Department of Obstetrics and Gynecology the Second Affiliated Hospital of Wenzhou Medical University

Background:Uterine fibroids are the most common benign gynecologic tumors in reproductive age ***,their origin and development is still not completely *** D is a necessary vitamin for the human ***,awareness is growing that vitamin D is associated with uterine *** these researches were all aimed at black and white people,no Chinese were ***,the relationship between serum calcium and uterine fibroids is rarely ***:In the present study,we carried out a case-control study of women attended the Second Affiliated Hospital of Wenzhou Medical University,to investigate the association of serum vitamin D,calcium levels with the occurrence and development of uterine fibroids in reproductive-aged *** part A,between August 2016 and December 2016,279 women who diagnosed with fibroids by ultrasound were eligible as fibroids *** 267 women without fibroids were recruited as non-fibroids *** participant provided a blood sample for the quantitative detection of serum 25-hydroxyvitamin D(25 OHD) and calcium *** part B,27 patients with fibroids who underwent the surgical removal of uterine fibroids(either myomectomy,or hysterectomy,or hysteroscopy) from September 2016 to December 2017 were selected as the fibroids ***,27 fibroid-free women with adnexal masses underwent oophorocystectomy were selected as the control *** the blood was collected for serum 25 OHD and calcium both before and two days after *** the enzyme linked immunosorbent assay was used to detect the levels of serum 25 *** photometric colorimetric method was utilized to detect the serum calcium ***:In part A,of the 546 participants,only 232(42.49%) women had sufficient 25 OHD levels(>20 ng/ml).Women with uterine fibroids had lower serum 250 HD,and women with insufficient/deficient 25 OHD(≤20 ng/ml) had an estimated 86% higher odds of fibroids compared with those 25 OHD sufficient.(OR=

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尿石素A对转化生长因子-β1诱导的人近端肾小管上皮细胞纤维化的影响

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中国食品添加剂 2023年第5期34卷 57-69页

作者：李佳婷李佳李云龙韩林王敏西北农林科技大学食品科学与工程学院杨陵712100 山西农业大学山西功能食品研究所太原030031

为探究尿石素A(urolithin A,UA)对人近端肾小管上皮细胞(HK-2)纤维化的干预效果和潜在作用机制,本研究建立转化生长因子-β1(transforming growth factor-β1,TGF-β1)诱导的HK-2纤维化模型,考察对照组、TGF-β1模型组(4 ng/mL处理48 h)... 详细信息

为探究尿石素A(urolithin A,UA)对人近端肾小管上皮细胞(HK-2)纤维化的干预效果和潜在作用机制,本研究建立转化生长因子-β1(transforming growth factor-β1,TGF-β1)诱导的HK-2纤维化模型,考察对照组、TGF-β1模型组(4 ng/mL处理48 h)、UA低浓度组(5μg/mL UA+4 ng/mLTGF-β1)、UA高浓度组(10μg/mL UA+4 ng/mLTGF-β1)对HK-2细胞的影响。试验结果表明:UA干预能改善TGF-β1引起的HK-2形态变化并显著降低细胞纺锤体指数(P<0.01);降低纤维化标物Col I、Col III、Vimentin、Snail和Slug和炎症标志物IL-1β、IL-6、MCP-1、NLRP3的表达(P<0.01);下调MAPK和TGF-β1/Smads信号通路相关蛋白的相对表达(P<0.01)。上述研究表明UA能减轻TGF-β1诱导的HK-2细胞纤维化,其作用的机制可能是通过下调MAPK和TGF-β1/Smads信号通路来实现的。本研究能为开发天然活性成分UA用于延缓肾纤维化提供新方法。

关键词：尿石素A 纤维化转化生长因子-β1 HK-2 MAPK信号通路

超高效液相色谱法同时测定降糖类保健食品中非法添加的13种化学药物

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食品科学 2016年第18期37卷 178-184页

作者：林芳李涛王一欣张丽刘海静陕西省食品药品检验所陕西西安710065

建立超高效液相色谱法,同时测定降糖类保健食品中可能非法添加的13种化学药物（盐酸二甲双胍、盐酸丁二胍、盐酸苯乙双胍、甲苯磺丁脲、格列吡嗪、格列齐特、格列波脲、盐酸罗格列酮、格列本脲、盐酸吡格列酮、格列美脲、瑞格列奈、格... 详细信息

建立超高效液相色谱法,同时测定降糖类保健食品中可能非法添加的13种化学药物（盐酸二甲双胍、盐酸丁二胍、盐酸苯乙双胍、甲苯磺丁脲、格列吡嗪、格列齐特、格列波脲、盐酸罗格列酮、格列本脲、盐酸吡格列酮、格列美脲、瑞格列奈、格列喹酮）。样品采用甲醇提取,经ZORBAX SB-C18色谱柱分离,乙腈和0.02 mol/L乙酸铵为流动相梯度洗脱,流速0.2 mL/min。采用二极管阵列检测器,采集波长235 nm处的色谱图进行初筛和定量。并通过与13种降糖药物标准品的光谱比对进行确证。结果表明,13种降糖药物在30 min内可有效分离,在0.3-90μg/mL的线性范围内相关系数均大于0.999,平均回收率为83.2%-108.9%,相对标准偏差均不大于5.5%,检出限为0.2-0.6 ng。该方法前处理简单、分析时间相对较短、灵敏度高且重复性好,实现一个系统对13种降糖药物的同时检测,可作为降糖类保健食品检测非法添加化学成分的参考方法。

关键词：保健食品降糖类药物非法添加超高效液相色谱法

Two dimensional oriented growth of Zn-MOF-on-Zr-MOF architecture: A highly sensitive and selective platform for detecting cancer markers

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Two dimensional oriented growth of Zn-MOF-on-Zr-MOF architec...

河南省化学会2018年学术年会

作者： zhenzhen li linghao He Minghua Wang Qiaojuan Jia zhihong zh.ng Henan Provincial Key Laboratory of Surface and Interface Science Zhengzhou University of Light Industry

Fabricating novel bimetallic metal organic framework(MOF) architectures and exploiting them as the aptasensor scaffolds for detecting diverse analytes, especially the cancer markers, have aroused researcher’s attention. We reported a novel strategy for the Zn Zr bimetallic MOFs preparation by the method of MOF-on-MOF and investigated their different aptamer strands immobilization and analyte detection behaviors. The basic characterizations reveal that the chemical structure, crystalline, and surface functionalinality of bimetallic ZnZr-MOFs can be modulated by changing the adding order of the metal precursors and ligands. It shows that the Zn-MOF-on-Zr-MOF exhibits the hierarchically decussated foliace, whereas the Zr-MOF-on-Zn-MOF demonstrates the multilayered nanosheets structure. The electrochemical results explore that the Zr-MOF facilitates the aptamer strands immobilization, while the Zn-MOF can stabilize the formed G-quadruplex of aptamer strands and PTK7. Consequently, the proposed Zn-MOF-on-Zr-MOF-based aptasensor possesses outstanding sensing performances toward PTK7, giving a ultralow detection limitation of 0.66 fg?mL-1 within the PTK7 concentration range from 0.001 to 1 ng?mL-1. Also, the proposed aptasensor exhibits high selectivity in the presence of coexistence of various interferents, good stability and reproduability, along with the acceptability in the human serum. The present strategy can provide a new approach for fabricating ultrasensitive and selective bimetallic MOFs-based aptasensors and broaden their applications in the early diagnosis fields toward cancer markers.

关键词： Bimetallic ZnZr-metal organic frameworks Electrochemical aptasensor Detection of protein tyrosine kinase-7 MOF-on-MOF composite

Water-soluble secondary organic aerosol in Qinghai Lake:implication for sources,formation,and degradation during long-range transport

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Water-soluble secondary organic aerosol in Qinghai Lake:impl...

十一届全国气溶胶会议暨第十届海峡两岸气溶胶技术研讨会

作者： Jingjing Meng Gehui Wang Jianjun li Chunlei Cheng Junji Cao State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of Sciences School of Human Settlements and Civil Engineering Xi’an Jiaotong University

PM2.5 aerosol sampling for collection of 24 h samples was carried out every day from Qinghai Lake（3200m a.s.l.）, a remote continental site in the northeastern part of Tibetan Plateau from July to August 2010. To better understand the production of water-soluble organic aerosols, the samples were analyzed for water-soluble dicarboxylic acids（C2-C11） and related compounds（ketocarboxylic acids and α-dicarbonyals）, as well as organic carbon（OC）, elemental carbon（EC）, water-soluble organic carbon（WSOC）, and water-soluble inorganic ions. Distributions of dicarboxylic acids and related compounds were characterized by a predominance of oxalic acid（29.3 – 347.6 ng m-3, 148.2 ± 83.9 ng m-3） followed by malonic（9.3 – 77.6 ng m-3, 27.2 ± 15.5 ng m-3） and succinic（3.6 – 29.3 ng m-3, 14.5 ± 8.0 ng m-3） acids. The average contributions of diacids, ketoacids and dicarbonyls to WSOC were 44.1%, 1.3%, and 0.4 %, respectively. They were several times higher than those reported in north-western cities from which air masses were transported to the Qinghai Lake, indicating an importance of photochemical processing of aerosols during a long-range transport. The diacids/WSOC and C9/diacids ratios were higher than those reported previously in different atmosphere aerosols, which may indicate that the local aerosols were chiefly influenced by the biomass burning（herbages and dung） and photochemical oxidations were enhanced because of the stronger solar radiation. This study demonstrated that diacids were primarily produced by the secondary photochemical oxidation of organic pollutants and ω-oxoacids were likely intermediates to the production of dicarboxylic acids.

关键词： Oxalic acid secondary organic aerosols PM 2.5 Qinghai Lake