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Electrical stimulation with polypyrrole-coated polycaprolactone/silk fibroin scaffold promotes sacral nerve regeneration by modulating macrophage polarisation

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Biomaterials Translational 2024年第2期5卷 157-174页

作者： Cheng H. Bai J. Zhou X. Chen N. Jiang Q. Ren Z. li X. Su T. liang l. Jiang W. Wang Y. Peng J. Shang A. School of Medicine Nankai University Tianjin China Department of Neurosurgery Chinese PLA General Hospital Beijing China Institute of Orthopedics Chinese PLA General Hospital Beijing Key Lab of Regenerative Medicine in Orthopedics Key Laboratory of Musculoskeletal Trauma and War Injuries PLA Beijing China Co-innovation Center of Neuroregeneration Nantong University Jiangsu Province Nantong China Graduate School of Chinese PlA General Hospital Beijing China Department of Ultrasound Chinese PLA General Hospital Beijing China

Peripheral nerve injury poses a great threat to neurosurgery and limits the regenerative potential of sacral nerves in the neurogenic bladder. It remains unknown whether electrical stimulation can facilitate sacral nerve regeneration in addition to modulate bladder function. The objective of this study was to utilise electrical stimulation in sacra nerve crush injury with newly constructed electroconductive scaffold and explore the role of macrophages in electrical stimulation with crushed nerves. As a result, we generated a polypyrrole-coated polycaprolactone/silk fibroin scaffold through which we applied electrical stimulation. The electrical stimulation boosted nerve regeneration and polarised the macrophages towards the M2 phenotype. An in vitro test using bone marrow derived macrophages revealed that the pro-regenerative polarisation of M2 were significantly enhanced by electrical stimulation. Bioinformatics analysis showed that the expression of signal transducer and activator of transcriptions (STATs) was differentially regulated in a way that promoted M2-related genes expression. Our work indicated the feasibility of electricals stimulation used for sacral nerve regeneration and provided a firm demonstration of a pivotal role which macrophages played in electrical stimulation. © 2024, Chinese Medical Multimedia Press Co ltd. All rights reserved.

关键词： electrical stimulation JAK-STAT signalling pathway macrophage polarisation peripheral nerve regeneration polypyrrole sacral nerve injury

Beyond the stomach: An updated view of Helicobacter pylori pathogenesis, diagnosis, and treatment

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World Journal of Gastroenterology 2014年第36期20卷 12781-12808页

作者： Traci l Testerman James Morris Department of Microbiology and Immunology Uniformed Services University of the Health SciencesBethesda MD 20814 United States Department of Gastroenterology Louisiana State University Health Sciences Center-Shreveport Shreveport LA 71130 United States

Helicobacter pylori (H. pylori) is an extremely common, yet underappreciated, pathogen that is able to alter host physiology and subvert the host immune response, allowing it to persist for the life of the host. H. pylori is the primary cause of peptic ulcers and gastric cancer. In the United States, the annual cost associated with peptic ulcer disease is estimated to be $6 billion and gastric cancer kills over 700000 people per year globally. The prevalence of H. pylori infection remains high (> 50%) in much of the world, although the infection rates are dropping in some developed nations. The drop in H. pylori prevalence could be a double-edged sword, reducing the incidence of gastric diseases while increasing the risk of allergies and esophageal diseases. The list of diseases potentially caused by H. pylori continues to grow; however, mechanistic explanations of how H. pylori could contribute to extragastric diseases lag far behind clinical studies. A number of host factors and H. pylori virulence factors act in concert to determine which individuals are at the highest risk of disease. These include bacterial cytotoxins and polymorphisms in host genes responsible for directing the immune response. This review discusses the latest advances in H. pylori pathogenesis, diagnosis, and treatment. Up-to-date information on correlations between H. pylori and extragastric diseases is also provided.

关键词： Enterohepatic Pathogenesis Diagnosis Treatment Extragastric CagA Cancer Autoimmune Inflammation virulence factor

Safety assessment methodology for a German high-level waste repository in clay formations

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Journal of Rock Mechanics and Geotechnical Engineering 2017年第5期9卷 856-876页

作者： M.Jobmann A.Bebiolka v.Burlaka P.Herold S.Jahn A.lommerzheim J.MaBmann A.Meleshyn S.Mrugalla K.Reinhold A.Rübel l.Stark G.Ziefle DBE TECHNOlOGY GmbH Eschenstra 55 Peine D-31224 Germany Bundesanstalt für Geowissenschaften und Rohstoffe (BGR) Stilleweg 2 Hannover D-30655 Germany Gesellschaft fur Anlagen- und Reaktorsicherheit (GRS) gGmbH Theodor-Heuss-Stra 4 Braunschweig D-38122 Germany

In the ANSICHT project that was jointly carried out by DBE TECHNOlOGY Gmb H,BGR,and GRS g Gmb H,two generic geological site models were used to develop a first draft of a methodology to demonstrate the safety of a high-level waste(HlW) repository in argillaceous formations in Germany,taking into account the regulatory *** main results of the project are characterised by the developed repository concepts adapted to the geological *** specific quantifications of the integrity criteria and their exemplary application with calculational proofs were used to demonstrate the integrity of the host *** development of site-specific FEP(features,events,and processes) cataloges provided a complete system description for evaluation of the repository *** developed work flow of the demonstration concept illustrated the complete sequence of the safety proof in a transparent *** shows that various steps have to be performed,possibly iteratively,to provide a successful safety *** results form a useful tool in the pending search for a HlW repository site,especially when providing a basis for comparing safety analyses of different sites in Germany.

关键词： Clay Radioactive waste Safety demonstration method Barrier integrity FEP(features events and processes) cataloge

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全补偿式弹性的储油罐密封结构改进设计

机电工程技术 2024年第7期53卷 264-268,274页

作者：李祥宾朱雅宋吕明连云港市拓普科技发展有限公司江苏连云港222069 江苏海洋大学机械工程学院江苏连云港222000 江苏海洋大学海洋工程学院江苏连云港222000

储油罐作为存储能源资源的重要设施,在密封性能方面要求极高,深入探讨了储油罐密封的重要性,以及提升其密封性能方法。首先,对储油罐密封的发展背景以及储油密封技术发展的重要性进行阐述;其次,从国内常用的储油罐密封结构普通机械鞋型... 详细信息

储油罐作为存储能源资源的重要设施,在密封性能方面要求极高,深入探讨了储油罐密封的重要性,以及提升其密封性能方法。首先,对储油罐密封的发展背景以及储油密封技术发展的重要性进行阐述;其次,从国内常用的储油罐密封结构普通机械鞋型密封结构、囊式弹性密封结构、全补偿式弹性密封结构的密封结构、使用范围、密封效果、优势与劣势方面进行论述;系统地探讨和论述全补偿式弹性密封结构在储油罐密封中的结构原理与优势,并提出了一系列改进方案。最后,通过实验论证所改进的全补偿式弹性密封结构的可靠性。通过对现有技术的整合和创新,提高了储油罐的密封性能,减少了资源浪费和环境污染,为储油罐密封技术的进一步发展提供有益的参考和借鉴。

关键词：油罐密封结构全补偿式弹性密封结构普通机械鞋型密封结构囊式弹性密封结构

Critical movement of large rocks in currents and waves

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International Journal of Sediment Research 2019年第4期34卷 387-398页

作者： l.C.van Rijn lvR-Sediment Consultancy

Cobbles, boulders, and rocks often are used in a bed protection layer near a structure to protect the underlying sand bed against erosion by combined current and waves. The design of a bed protection layer consisting of loose rocks(rubble mound) requires knowledge of the stability and movement(as bed load) of very coarse materials. If some movement(or damage) is acceptable, the rock diameter can be designed to be smaller. This paper addresses the stability and movement of very coarse materials(cobbles, boulders, and rocks) based on the concept of the critical Shields mobility number. It is shown that the bed load transport of large cobbles, boulders, and rocks can be described by the equations of Meyer-Peter and Mueller(MPM) and Cheng. Both are valid for relatively small Shields mobility *** and general equations for the design of a bed protection layer(including some permissible damage)in conditions with a current with or without waves are proposed based on the Shields mobility parameter and the bed load transport equation of Cheng. laboratory and field data of critical velocities for pebbles, cobbles, boulders, and rocks have been analyzed and compared to the computed results of the proposed equations. Practical applications are given to demonstrate the general applicability of the proposed equations.

关键词： Rock stability Bed protection Critical movement Bed load transport Rocks

Mutation in the leucinerich repeat of platelet glycoprotein Ibαresults in defects in its interaction with immobilized von Willebrand factor under flow

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Chinese Medical Journal 2000年第8期113卷 21-26页

作者：董京飞 Chesterli Alicia J.Schade Becky J.Fredrickson lily Sun larry v.McIntire José A.lópez From the Division of Hematology/Oncology Department of Internal Medicine Cox Laboratory for Bioengineering Rice University Houston TX 77030 USA Department of Molecular and Human Genetics Baylor College of Medicine and Veterans Affairs Medical Center Houston Cox Laboratory for Bioengineering Rice University Houston TX 77030 USA

ObjectiveTo characterize effects of the GP Iba mutation(A156v)on its interaction with von Willebrand factor(vWf)under high fluid shear stress.MethodsThe residue A156 of GP Iba was converted to a valine and the mutant expressed in CHO cells expressing wild-type GP Ibβ and GPIX. The transfected cells were tested for their interaction with a panel of GP Iba antibodies and for rolling on immobilized vWf under high shear.ResultsThe mutation led to surface expression of a GP Iba polypeptide that adopted a different conformation at its N-terminus because binding of the GP Iba antibody AN51, which has a binding epitope it the N-terminal 35 residues, was eliminated, whereas binding of the others(AK2, MB45, and SZ2, all of which bind to regions C-terminal to the AN51 epitope)was normal. Mutant-expressing cells could adhere and roll on immobilized vWf under high fluid shear stress and rolled significantly faster than wild-type cells.ConclusionThese studies demonstrate that the mutation A156v results in a conformation change at the N-terminus of GP Ibα, which leads to an increase in the dissociation rate of the bond between the GP Ibα mutant and vWf. Chin Med J 2000; 113(8): 693-698

关键词： mutation glycoprotein von Willebrand factor defects

Behavioral and olfactory responses of grasshopper hatchlings, Melanoplus sanguinipes, to plant odours and volatile compounds

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Chinese Science Bulletin 2004年第2期49卷 136-141页

作者： KANGle T.l.Hopkins StateKeylaboratoryofIntegratedManagementofPestInsects&Rodents.InstituteofZoology ChineseAcademyofSciencesBeijing100080China DepartmentofEntomology KansasStateUniversityManhattanKS66506USA

Behavior and olfactory responses of grasshopper hatchlings, Melanoplus sanguinipes (F.), to odours from plant foliage and volatile compounds were tested using a glass Y-tube olfactometer and electroantennogram (EAG)techniques respectively. In single choice trials, newly hatched hoppers were much more sensitive to the odour from intact leaves and chopped foliage of ryegrass and wheat than other plants. Chopped sorghum leaves, but not stem-cut sorghum,were also significantly attractive. The orientation responses of grasshopper hatchlings to these plants were highly consistent with those of last instar hoppers and adults. When ryegrass was employed as the control, the odour from stem-cut alfalfa was more attractive. There was no significant difference in hopper orientation responses to the odours from chopped seedlings of sorghum, alfalfa, wheat or ***, significantly more hoppers preferred the chopped ryegrass control to chopped louisanna sage. Measurement of the EAG response of first instar hoppers to these plant odours showed that the odour of louisanna sage elicited the greatest response amplitudes. In olfactory tests using different volatile components, Z-3-hexenol, E-3-hexenol,Z-hex-3-enyl acetate, E-2-hexenal and hexenal gave greater EAG responses than geraniol and 1-octen-3-ol. These results are also consistent with comparable data from adults. Newly hatched grasshoppers had similar EAG response profiles to plant materials and chemicals to those of adults, although the absolute EAG values of young hoppers were much lower than those of adults. Therefore, newly hatched hoppers were able to distinguish plants from an air control, and even host plants from non-host plants, and the feeding experience of hoppers probably has little influence on their subsequent ability as adults to identify and locate food plants.

关键词：嗅觉蝗虫挥发性化合物植物保护

Determination of genotoxic alkyl methane sulfonates and alkyl paratoluene sulfonates in lamivudine using hyphenated techniques

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Journal of Pharmaceutical Analysis 2012年第4期2卷 314-318页

作者： N.v.v.S.S.Raman A.v.S.S.Prasad K.Ratnakar Reddy K.Ramakrishna Hetero Drugs ltd.(R&D) GITAM Institute of Science GITAM University

Two highly sensitive methods for the determination of genotoxic alkyl methane sulfonates (AMSs) and alkyl paratoluene sulfonates (APTSs) in lamivudine using hyphenated techniques have been presented. AMSs were determined by GC-MS method using GSBPINOWAX (30 m 0.25 mm 0.25 mm) column. Temperature program was set by maintaining at 100 1C initially for 3 min, then rised to 220 1C at the rate of 15 1C/min and maintained at 220 1C for 16 min. N,N-dimethyl formamide was used as diluent. APTSs were determined by lC-MS using Zorbax, Rx C8, 250 mm 4.6 mm, 5 mm column as stationary phase. 0.01 M ammonium acetate is used as buffer. The mixture of buffer and methanol in 75:25 (v/v) ratio was used as mobile phase A and mixture of buffer and methanol in 5:95 (v/v) ratio was used as mobile phase B. The gradient program (T/%B) was set as 0/28, 16/50, 17/100, 23/100, 27/28 and 40/28. Both the methods were validated as per International Conference on Harmonization guidelines. limit of quantitation was found 1.5 mg/ml for AMSs and was in the range of 1.0-1.5 mg/ml for APTSs.

关键词： Genotoxins Alkyl methane sulfonates Alkyl paratoluene sulfonates lamivudine Hyphenated techniques

Theoretical study of defect impact on two-dimensional MoS

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Journal of Semiconductors 2015年第12期 22-27页

作者： Anna v.Krivosheeva victor l.Shaposhnikov victor E.Borisenko Jean-louis lazzari Chow Waileong Julia Gusakova Beng Kang Tay Belarusian State University of Informatics and Radioelectronics CNRS Aix-Marseille Université NOvITAS Nanoelectronics Centre of Excellence School of Electrical and Electronic Engineering Nanyang Technological University CINTRA CNRS NTUTHALES Nanyang Technological University

Our theoretical findings demonstrate for the first time a possibility of band-gap engineering of monolayer MoS;crystals by oxygen and the presence of vacancies. Oxygen atoms are revealed to substitute sulfur ones, forming stable MoS;O;ternary compounds, or adsorb on top of the sulfur atoms. The substituting oxygen provides a decrease of the band gap from 1.86 to 1.64 ev and transforms the material from a direct-gap to an indirect-gap semiconductor. The surface adsorbed oxygen atoms decrease the band gap up to 0.98 ev depending on their location tending to the metallic character of the electron energy bands at a high concentration of the adsorbed atoms. Oxygen plasma processing is proposed as an effective technology for such band-gap modifications.

关键词： two-dimensional crystal molybdenum disulfide band gap vacancy oxygen