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邻苯二甲酸单丁酯通过Caspase-3/GSDME通路诱导睾丸间质细胞焦亡

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宁夏医科大学学报 2024年第1期46卷 15-20页

作者：闫凤梅李玲郝羽黄静撒开清王硕宁夏医科大学公共卫生学院职业卫生与环境卫生学系银川750004 宁夏环境因素与慢性病控制重点实验室银川750004

目的探讨邻苯二甲酸单丁酯(monobutyl phthalate,MBP)诱导睾丸间质细胞(TM-3细胞)焦亡及其可能的发生机制。方法用浓度为0、2.5、5、10 mmol·l^(-1) MBP(对照组,低剂量组,中剂量组,高剂量组)染毒对数生长期细胞24 h后,光学显微镜观察... 详细信息

目的探讨邻苯二甲酸单丁酯(monobutyl phthalate,MBP)诱导睾丸间质细胞(TM-3细胞)焦亡及其可能的发生机制。方法用浓度为0、2.5、5、10 mmol·l^(-1) MBP(对照组,低剂量组,中剂量组,高剂量组)染毒对数生长期细胞24 h后,光学显微镜观察细胞形态学变化;微量酶标法检测乳酸脱氢酶(lactate dehydrogenase,lDH)的释放率;荧光显微镜检测PI阳性细胞比例;Western blot检测焦亡相关蛋白Caspase-3、GSDME的相对表达水平。将对数生长期的TM-3细胞暴露于浓度梯度(0、2.5、5、10、20μmol·l^(-1))的Caspase-3抑制剂Ac-DEVD-CHO中24 h,CCK-8法检测细胞活力,确定Ac-DEVD-CHO染毒剂量。Ac-DEVD-CHO作用于细胞验证MBP诱导TM-3细胞焦亡的机制。结果与对照组相比,各染毒组细胞lDH的释放率、PI阳性细胞比例以及Caspase-3和GSDME蛋白的相对表达水平均升高(P均l·l^(-1)时,细胞存活率为99.17%(P>0.05),并且中剂量+抑制剂组的细胞存活率较中剂量组升高(Pl·l^(-1)。并且中剂量+抑制剂组的lDH的释放率、PI阳性细胞比例以及Caspase-3和GSDME蛋白的相对表达水平较中剂量组下降(P均<0.05)。结论MBP可以通过Caspase-3/GSDME通路诱导睾丸间质细胞焦亡。

关键词：细胞焦亡睾丸间质细胞 Caspase-3蛋白 GSDME蛋白

Petrologic composition model of the upper crust in Bohai Bay basin,China,based on lamé impedances

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Applied Geophysics 2009年第4期6卷 327-336,393,394页

作者：张唽 loui sa l.H.Tsang 王仰华赵兵中科院地质与地球物理研究所岩石圈演化国家重点实验室中国北京100029 Centre for Reservoir Geophysics Department of Earth Science and EngineeringImperial CollegeLondon

Seismic attributes, such as P- and S-wave velocity, Poisson＇s ratio, and acoustic impedances, all generally can be used for distinguishing different rock types. The nonuniqueness can be largely reduced using lame impedances instead of acoustic impedances as additional constraints. We have followed this method to constitute a petrologic composition model of the upper crust in the Bohai Bay basin, China. We briefly review the seismic parameters used for discrimination of rock types and focus our attention on the sensitivity of different combinations of parameters to determine the composition of materials. Corrections for pressure and temperature are performed in order to compare elastic wave velocities and densities measured at room temperature and surface pressure in laboratory with those for representative rock parameters. In a second step, we find the rock classes in the tested area by contrasting known data to laboratory measurements on a variety of rock samples extracted in the area. The basic field data are P-wave velocity values collected along a seismic profile conducted in the Bozhong Depression. The different rock types belonging to a particular rock class are finally constrained by the seismic velocities, Poisson＇s ratio, density, acoustic impedance, and lame imoedance related to the topmost 10 km of the Bohai Bav crust.

关键词： seismic velocity Poisson's ratio acoustic and lam6 impedances Bohai Bay

Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension

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Journal of Integrative Medicine 2016年第2期14卷 100-113页

作者： Nathalia Gomes Ribeiro de Moura Ivan Cordovil Arthur de sa Ferreira Postgraduate Program of Rehabilitation Science Augusto Motta University Center21041-010Rio de JaneiroR JBrazil Division of Arterial Hypertension National Institute of Cardiology22240-002Rio de JaneiroR JBrazil Department of Physical Therapy Salgado de Oliveira University24030-060NiteroiR JBrazil

BACKGROUND： Pulse wave analysis（PWA） quantifies the phenomenon of pulse waveform propagation in patients with cardiovascular diseases, whereas pulse image analysis（PIA） is a subjective examination in traditional Chinese medicine. OBJECTIVE： This study evaluated the association of PIA with PWA and hemodynamics in patients with hypertension. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： This observational, cross-sectional study enrolled 45 patients（26 men,（55.2± 10.3） years, systolic blood pressure（155± 28） mm Hg, diastolic blood pressure（93± 17） mm Hg） for assessment of clinical and laboratorial data. MAIN OUTCOME MEASURES： Primary outcomes comprised： pattern differentiation based on an automated method; PIA at the radial artery using the ‘simultaneous pressing＇ method for identification of factors such as strength（strong/weak）, depth（superficial/deep）, and speed（fast/moderate/slow）; and PWA at the same artery using a noninvasive system. RESUlTS： Significant multivariate main effects were observed for depth（l=0.648, F_（5,29）=3.149, P=0.022, h^2 =0.352）, strength（l=0.608, F_（5,29） =3.736, P=0.010, h^2 =0.392）, and speed（l=0.535, F_（5,29） =5.302, P=0.002, h^2 =0.465）. General effects comprised high values of PWA and blood pressure for superficial, strong, and fast pulse images. A strong pulse was found for pulse pressure ≥ 62.5 mm Hg and systolic blood pressure ≥ 149.5 mm Hg, whereas a superficial pulse was found for heart rate ≥ 58.25 beats/min; a fast pulse was found for heart rate ≥ 69.6 beats/min and pulse wave velocity ≥ 9.185 m/s. CONClUSION： Associations were explained by la Place＇s law, arterial remodeling in hypertension, alongside the traditional criterion for classifying speed in pulse images. PIA is associated with PWA and hemodynamics in patients with hypertension. Systolic and pulse pressures, heart rate, and pulse wave velocity are quantitative variables that have information to describe the qualitative pulse images such as strength, depth and speed.

关键词： medicine Chinese traditional hypertension syndrome differentiation rehabilitation

Dual-Functional lithiophilic/Sulfiphilic Binary-Metal Selenide Quantum Dots Toward High-Performance li-S Full Batteries

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Nano-Micro letters 2023年第5期15卷 169-186页

作者： Youzhang Huang liang lin Yinggan Zhang lie liu Baisheng sa Jie lin laisen Wang Dong-liang Peng Qingshui Xie State Key lab for Physical Chemistry of Solid Surfaces Fujian Key Laboratory of Surface and Interface Engineering for High Performance MaterialsCollege of MaterialsXiamen UniversityXiamen 361005People’s Republic of China Shenzhen Research Institute of Xiamen University Shenzhen 518000People’s Republic of China College of Materials Science and Engineering Multiscale Computational Materials FacilityFuzhou UniversityFuzhou 350100People’s Republic of China

The commercial viability of lithium-sulfur batteries is still challenged by the notorious lithium polysulfides(li PSs)shuttle effect on the sulfur cathode and uncontrollable li dendrites growth on the li ***,a bi-service host with Co-Fe binary-metal selenide quantum dots embedded in three-dimensional inverse opal structured nitrogen-doped carbon skeleton(3DIO FCSe-QDs@NC)is elaborately designed for both sulfur cathode and li metal *** highly dispersed FCSe-QDs with superb adsorptive-catalytic properties can effectively immobilize the soluble li PSs and improve diffusion-conversion kinetics to mitigate the polysulfide-shutting ***,the 3D-ordered porous networks integrated with abundant lithophilic sites can accomplish uniform li deposition and homogeneous li-ion flux for suppressing the growth of *** advantage of these merits,the assembled li-S full batteries with 3DIO FCSe-QDs@NC host exhibit excellent rate performance and stable cycling ability(a low decay rate of 0.014%over 2,000 cycles at 2C).Remarkably,a promising areal capacity of 8.41 mAh cm^(-2)can be achieved at the sulfur loading up to 8.50 mg cm^(-2)with an ultra-low electrolyte/sulfur ratio of 4.1μl mg^(-1).This work paves the bi-serve host design from systematic experimental and theoretical analysis,which provides a viable avenue to solve the challenges of both sulfur and li electrodes for practical li-S full batteries.

关键词： Dual-functional host Fe_(2)CoSe_(4)quantum dots Shuttle effect Dendrite-free li anode li-S full batteries

Harnessing the power of complex light propagation in multimode fibers for spatially resolved sensing

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Photonics Research 2024年第3期12卷 411-422页

作者： DARCY l.SMITH lINH V.NGUYEN MOHAMMAD I.REJA ERIK P.SCHARTNER HEIKE EBENDORFF-HEIDEPRIEM DAVID J.OTTAWAY STEPHEN C.WARREN-SMITH Institute for Photonics and Advanced Sensing and School of Physics Chemistry and Earth SciencesThe University of AdelaideAdelaideSA 5005Australia Australian Research Council Centre of Excellence for Gravitational Wave Discovery(OzGrav) AdelaideSAAustralia Future Industries Institute University of South AustraliaMawson LakesSA 5095Australia laser Physics and Photonics Devices laboratory University of South AustraliaMawson LakesSA 5095Australia

The propagation of coherent light in multimode optical fibers results in a speckled output that is both complex and sensitive to environmental *** properties can be a powerful tool for sensing,as small perturbations lead to significant changes in the output of the ***,the mechanism to encode spatially resolved sensing information into the speckle pattern and the ability to extract this information are thus far *** this paper,we demonstrate that spatially dependent mode coupling is crucial to achieving spatially resolved *** leverage machine learning to quantitatively extract the spatially resolved sensing information from three fiber types with dramatically different characteristics and demonstrate that the fiber with the highest degree of spatially dependent mode coupling provides the greatest accuracy.

关键词： fiber spatially resolved

Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat

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Journal of Integrative Agriculture 2013年第8期12卷 1441-1449页

作者： BAI Jian-hui lIU Jing-hui ZHANG Na YANG Jun-heng sa Ru-la WU lan Science Innovation Team of Oat Inner Mongolia Agricultural University College of Foreign languages Inner Mongolia Agricultural University

Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were：（i） low concentrations of alkali stress （25 and 50 mmol l^-1） significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde （MDA） content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase （SOD）, and peroxidase （POD） activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; （ii） soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; （iii） alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; （iv） under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress,

关键词： oat alkali stress MDA soluble sugar antioxidant enzyme yield

Whole body vibration and auriculotherapy improve handgrip strength in individuals with knee osteoarthritis

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Journal of Traditional Chinese Medicine 2019年第5期39卷 707-715页

作者： Eloa Moreira-Marconi Carla F Dionello Danielle S Morel Danubia C sa-Caputo Cintia R Sousa-Goncalves laisa l Paineiras-Domingos Ygor Teixeira-Silva Mario Jose dos santos Pereira Mario Bernardo-Filho Postgraduate Program in Clinical and Experimental Physiopathology University of the State of Rio de JaneiroRio de JaneiroRJBrazil Department of Biophysics and Biometrics Laboratory of Mechanical Vibrations and Integrative Practices-LAVIMPIInstitute of Biology Roberto Alcantara GomesRio de Janeiro State UniversityRio de JaneiroRJBrazil Postgraduate Program in Medical Sciences Rio de Janeiro State UniversityRio de JaneiroRJBrazil Danubia C sa-Caputo Faculdade Bezerra de AraujoRio de JaneiroBrazilCentro Universitario Serra dos OrgaosTeresopolisRio de JaneiroBrasil Universidade do Estado do Rio de Janeiro Rio de JaneiroRJBrazil-Department of Biophysics and BiometricsInstitute of Biology Roberto Alcantara Gomes-Department of Physiological Sciences

OBJECTIVE: To verify the action of non-pharmacological interventions, whole body vibration exercise (WBVE) and auriculotherapy (AT) on the management of knee osteoarthritis (KOA) analyzing the handgrip strength (HS). METHODS: One hundred twelve participants with KOA were allocated in (a) WBVE group with peak-to-peak displacement of 2.5 to 7.5 mm, frequency from 5 up to 14 Hz, acceleration peak from 0.12 up to 2.95 g (2 d/weekly for 5 weeks),(b) AT group, points of both ears (Kidney, Knee-correspondent point and Shenmen) were stimulated with seeds,(c) WBVE + AT group and (d) respective control groups. HS was assessed in all the participants, in acute and cumulative responses. RESUlTS: The intervention with WBVE alone and combined with AT improved (P lative response, the HS. CONClUSION: WBVE alone or combined with AT might promote biological effects that interfere with the HS in individuals with KOA.

关键词： Osteoarthritis,knee Exercise therapy Physical and rehabilitation medicine Auriculotherapy Muscle strength dynamometer

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多数据源科研合作网络分析方法及实证研究

图书情报工作 2014年第18期58卷 89-94,109页

作者：王飒吕娜北京理工大学图书馆

认为已有科研合作网络分析研究成果多是基于某文献数据库单一数据源,无法全面完整地反映真实的科研合作状态。基于多数据源,从作者贡献、合著次数、合著强度和合著效果等角度对合著网络中的点权和边权进行加权,构建多数据源的科研合作... 详细信息

认为已有科研合作网络分析研究成果多是基于某文献数据库单一数据源,无法全面完整地反映真实的科研合作状态。基于多数据源,从作者贡献、合著次数、合著强度和合著效果等角度对合著网络中的点权和边权进行加权,构建多数据源的科研合作网络分析方法。实证分析表明,本文所提出的方法较基于单一数据源的合著分析能够真实、全面地反映科研合作状态。

关键词：多数据源合作网络合著网络加权融合点权边权

基于NMDAR1/cGMP通路探究“三法三穴”对轻度慢性压迫性神经损伤大鼠脊髓背角的镇痛启动机制

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北京中医药大学学报 2024年第7期47卷 1017-1024页

作者：杨震杰萨出拉于天源陈金平张润龙张英琦张汉钰孙佳伟刘家玥北京中医药大学针灸推拿学院北京102488

目的探究“三法三穴”推拿手法对轻度慢性压迫性神经损伤(minor CCI)模型大鼠的镇痛启动机制。方法 35只SD大鼠按照随机数字表法分为5组,即正常组、假手术组、模型组、推拿组、推拿+MK-801组。模型组、推拿组和推拿+MK-801组结扎右侧... 详细信息

目的探究“三法三穴”推拿手法对轻度慢性压迫性神经损伤(minor CCI)模型大鼠的镇痛启动机制。方法 35只SD大鼠按照随机数字表法分为5组,即正常组、假手术组、模型组、推拿组、推拿+MK-801组。模型组、推拿组和推拿+MK-801组结扎右侧坐骨神经干建立minor CCI大鼠模型;假手术组仅暴露右侧坐骨神经,不予结扎;正常组不予任何操作。正常组不予任何干预措施;模型组、假手术组造模后第7天予抓握束缚9 min;推拿组造模后第7天进行1次三法(点法、拨法、揉法)三穴(右侧“殷门”“承山”“阳陵泉”)干预,每法每穴干预1 min,共9 min;推拿+MK-801组造模后第5~7天鞘内注射MK-801,每次6μg(10μl),每日1次,最后一次鞘内注射30 min后再进行推拿干预,推拿具体操作同推拿组。造模前、造模后、干预后检测各组大鼠冷敏阈值(CST)和机械缩足反射阈值(MWT);干预后,免疫组织化学法检测腰4~6节段脊髓背角中环磷酸鸟苷(cGMP)蛋白阳性表达,蛋白质印迹法检测腰4~6节段脊髓背角中N-甲基-D-天冬氨酸受体1(NMDAR1)、神经源型一氧化氮合酶(nNOS)、可溶性鸟苷酸环化酶β(sGCβ)、蛋白激酶G1(PKG1)蛋白表达,实时荧光PCR法检测腰4~6节段脊髓背角中NMDAR1、nNOS、sGCβ、cGMP、PKG1 mRNA表达。结果与正常组、假手术组比较,造模后模型组、推拿组和推拿+MK-801组CST升高,MWT降低(均P<0.05);干预后模型组腰4~6节段脊髓背角中cGMP蛋白阳性表达增多,NMDAR1、nNOS、sGCβ、PKG1蛋白表达增多,NMDAR1、nNOS、sGCβ、cGMP、PKG1 mRNA表达增多(均P<0.05)。与模型组比较,干预后推拿组和推拿+MK-801组CST降低,MWT升高(均P<0.05);推拿组、推拿+MK-801组腰4~6节段脊髓背角中cGMP蛋白阳性表达减少,NMDAR1、nNOS、sGCβ、PKG1蛋白表达减少,NMDAR1、nNOS、sGCβ、cGMP、PKG1 mRNA表达减少(均P<0.05)。结论推拿干预1次后即可有效改善周围神经病理性疼痛引起的温度觉、机械痛觉过敏症状,可能是通过NMDAR1/cGMP/蛋白激酶G信号通路启动镇痛,从而发挥即刻镇痛作用。

关键词：推拿神经病理性疼痛 N-甲基-D-天冬氨酸受体1 脊髓背角镇痛大鼠