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Bull spermatozoa selected by thermotaxis exhibit high DNA integrity,specific head morphometry,and improve ICSI outcome

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1001-1011页

作者： Sara Ruiz-diaz Rosane Mazzarella Paula Navarrete-lopez Raul Fernandez-Gonzalez Celia de Frutos Maria Maroto Claudia Cucala Paula Beltran-Brena Marta lombo Dimitrios Rizos Alfonso Gutierrez-Adan Department of Animal Reproduction INIA-CSIC28040 MadridSpain Mistral Fertility Clinics S.l Clinica TambreCalle Tambre828002 MadridSpain

Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation(SDF)in mice,humans,and *** study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by *** We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TAlP medium supplemented with 25 mmol/l of HEPES and 5 mmol/l of *** these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 ***,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially *** The results showed lower SDF(χ^(2),Plower head size parameters(length and width,Plations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated *** performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),Plusion Our results showed that bull sperm selection by thermotaxis has a much higher D

关键词： ART Bovine Sperm morphometry Sperm selection Thermotaxis

Relevant Facts from Aerosol Measles Vaccine Studies

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World Journal of Vaccines 2016年第3期6卷 43-45页

作者： Fernández de Castro Jorge M. l. Garcia-Garcia Jose luis diaz-Ortega Deyanira Castaneda Instituto Nacional de Salud Pública Secretaría Nacional de Salud Pública Cuernavaca Mexico

Most of the facts regarding measles aerosol vaccination have been quite thoroughly dealt with in the book by S. Plotkin, Mass Vaccination: Global Aspects—Progress and Obstacles (2006 Ed) [1]. However, there are some aspects mentioned there that should perhaps have been emphasized more strongly and others that have emerged as relevant issues since then. We shall start with the quite important point that in the Sabin et al. [2] first study made in Monterrey, N. l., México [3], antibody reaction for recipients of respiratory-route Edmonston-Zagreb vaccines was not fully developed (100%) until six months after aerosol inhalation. At six weeks, only 90% had increased blood levels of antibody, a fact for which there is no easy explanation, but one which should be considered when dealing with serologic evaluation of measles vaccines given by aerosol in which shortterm results less than encouraging. Results of the second study performed by Sabin et al. in Monterrey [2] establish that the percentage of sero-responses is directly dose-dependent. In turn, the dose itself depends on at least three facts: 1) concentration of virus in the vaccine used;2) kind of nebulizer used;and 3) time of exposure. Another point to be stressed is that the vaccine used in the first trials [1]-[3], as well in the aerosol mass vaccination in México, though originally the Ickic strain attenuated in HDP, was also grown for final harvesting in HDP (MRC-5), whereas current Edmonston-Zagreb vaccines are obtained by final culture in chick embryo fibroblasts that provide 1 log more final product, more beneficial from an economic point of view, but not for adaptation to human tissues. A crucial consideration in aerosol measles mass campaigns is the lack of electricity/ energy supply, particularly in rural communities. To deal with these issues, a rudimentary assembly was utilized to produce the aerosol for mass vaccinations performed during the serious Mexican epidemic of 1990-1991: a tire pump connected

关键词： Immunology Immunopharmacology

Radiationless anapole states in on-chip photonics

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light(Science & Applications) 2021年第11期10卷 2161-2172页

作者： Evelyn diaz-Escobar Thomas Bauer Elena Pinilla-Cienfuegos Angela I.Barreda Amadeu Griol l.Kuipers Alejandro Martinez Nanophotonics Technology Center Universitat Politecnica de ValènciaCamino de Vera s/n46022ValenciaSpain Department of Quantum Nanoscience Kavli Institute of NanoscienceDelft University of Technology2600 GADelftThe Netherlands Institute of Applied Physics Abbe Center of PhotonicsFriedrich Schiller University JenaAlbert-Einstein-Straße 1507745JenaGermany

High-index nanoparticles are known to support radiationless states called anapoles,where dipolar and toroidal moments interfere to inhibit scattering to the far *** order to exploit the striking properties arising from these interference conditions in photonic integrated circuits,the particles must be driven in-plane via integrated ***,we address the excitation of electric anapole states in silicon disks when excited on-chip at telecom *** contrast to normal illumination,we find that the anapole condition-identified by a strong reduction of the scattering-does not overlap with the near-field energy maximum,an observation attributed to retardation *** experimentally verify the two distinct spectral regions in individual disks illuminated in-plane from closely placed waveguide terminations via far-field and near-field *** finding has important consequences concerning the use of anapole states and interference effects of other Mie-type resonances in high-index nanoparticles for building complex photonic integrated circuitry.

关键词： scattering waveguide states

Application of a biochemical and clinical model to predict individual survival in patients with end-stage liver disease

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World Journal of Gastroenterology 2009年第22期15卷 2768-2777页

作者： Eduardo Vilar Gomez luis Calzadilla Bertot Bienvenido Gra Oramas Enrique Arus Soler Raimundo llanio Navarro Javier diaz Elias Oscar Villa Jiménez Maria del Rosario Abreu Vazquez Department of Hepatology National Institute of Gastroenterology Department of Pathology National Institute of Gastroenterology Department of Gastroenterology National Institute of Gastroenterology Deparment of Gastroenterology The"Calixto Garcia"Hospital Department of Biostatistics National Institute of Gastroenterology

AIM:To investigate the capability of a biochemical and clinical model,BioCliM,in predicting the survival of cirrhotic ***:We prospectively evaluated the survival of 172 cirrhotic *** model was constructed using clinical(ascites,encephalopathy and variceal bleeding) and biochemical(serum creatinine and serum total bilirubin) variables that were selected from a Cox proportional hazards *** was applied to estimate 12-,52-and 104-wk *** model's calibration using the Hosmer-lemeshow statistic was computed at 104 wk in a validation ***,the model's validity was tested among an independent set of 85 patients who were stratified into 2 risk groups(low risk≤8 and high risk>8).RESUlTS:In the validation cohort,all measures of fi t,discrimination and calibration were improved when the biochemical and clinical model was *** proposed model had better predictive values(c-statistic:0.90,0.91,0.91) than the Model for End-stage liver Disease(MElD) and Child-Pugh(CP) scores for 12-,52-and 104-wk mortality,*** addition,the Hosmer-lemeshow(H-l) statistic revealed that the biochemical and clinical model(H-l,4.69) is better calibrated than MElD(H-l,17.06) and CP(H-l,14.23).There were no significant differences between the observed and expected survival curves in the stratified risk groups(low risk,P=0.61;high risk,P=0.77).CONClUSION:Our data suggest that the proposed model is able to accurately predict survival in cirrhotic patients.

关键词： liver cirrhosis Prognosis Statistical models Prognostic factors Model for end-stage liver disease score Child-Pugh score Survival

Evaluation of the Nutritional Supplement VIUSID Vet Powder on the Productive Behaviour of Sows and Boars

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Journal of Environmental Science and Engineering(B) 2016年第9期5卷 432-439页

作者： Juan Carlos Rodriguez-Femhndez Vicente Mendez-Garcia Ibrain calero-Herrera Kolima Pefia-Calzada Domingo Martos-Tejera Bulent Kukurtcu Department of Veterinary Medicine and Animal Husbandry University of Sancti Spiritus Sancti Spiritus 60100 Cuba Department of Production Swine Farm of Venega Yaguajay 64180 Cuba Scientific Department CATALYSIS S.L. Madrid28016 Spain

The objective of this research was to evaluate the effect of administering VIUSID vet powder on the productive behaviour of sows and the quality of semen of boars. Two experiments were conducted on farms in the province of Sancti Spiritus, in the central region of Cuba. One was with sows, starting 21 days before parturition until weaning, and another was with boars in directed mounting. Each sow received 10 grams of VIUSID vet powder everyday, mixed with food, from 21 days before the probable date of farrowing and until weaning （33-day lactation period）. In the boars, the treatment consisted of administering 10 grams of VIUSID vet powder with feed, once a day for 6 weeks （week 1 to 6）; from week 7 onwards, the supplement was suspended. Results showed that, supplying VIUSID vet powder significantly improved the principal post-partum productivity indicators and significantly reduced diarrhoea and administration to boars in production for 6 weeks, which has improved spermatic motility and does not affect the other variables representative of the quality of the semen.

关键词： VIUSID sows boars Glycyrrhizaglabra.

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车用液化气组分的优化

石化译文 2000年第2期 1-4页

作者： l.diaz等汪文强

选择不同的丙烷／丁烷混合物，对墨西哥市区以液化石油气（ＬＰＧ）为燃料的３种有代表性舞型进行试验。为确定墨西哥市区车用ＬＰＧ的标准，对尾气排放、烃含量和臭氧形成趋势等进行评价。试验结果表明，丙烷含量从６０v%上升到８５v%... 详细信息

选择不同的丙烷／丁烷混合物，对墨西哥市区以液化石油气（ＬＰＧ）为燃料的３种有代表性舞型进行试验。为确定墨西哥市区车用ＬＰＧ的标准，对尾气排放、烃含量和臭氧形成趋势等进行评价。试验结果表明，丙烷含量从６０v%上升到８５v%后，对一氧化碳和氮氧化物的降低有很好的作用，但对降低有机物的排放影响较小。从降低环境污染角度看，车用ＬＰＧ的最佳配方中的丙烷含量为７０１±4v%。

关键词：液化石油气车用燃料组分优化空气污染

Underwater video cameras allow for detection of North American giant salamanders (Cryptobranchus alleganiensis alleganiensis) in both captive and wild streams

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Aquaculture and Fisheries 2021年第1期6卷 106-110页

作者： Shem Unger Zeb Cline Hull lauren diaz John D.Groves lori A.Williams Catherine M.Bodinof Jachowski Carolina Headwaters L.L.C.MathewsNC28105United States Clemson University ClemsonSC29634United States North Carolina Zoological Park(Curator Emeritus) AsheboroNC27205United States North Carolina Wildlife Resources Commission RaleighNC27606United States

Captive propagation and translocation are becoming vital components of conservation and management strategies for Eastern hellbender(Cryptobranchus ***)***,aquaria,universities,and state and federal agencies are concomitantly collaborating on the protection,education,and maintenance of captive populations of this unusual,cryptic *** strategies include the use of artificial nesting structures,collection of eggs from the wild,and head-starting individuals in zoos or *** effects of these strategies need to be monitored,however,traditional survey and monitoring methods for the species in the wild involves rock-lifting,which has the potential to both harm habitat and alter reproductive ***,there is a need to develop effective,non-invasive and non-destructive methods of monitoring both wild and captive populations of Eastern ***,we compare two simple,affordable,underwater video and camera systems(borescope and Aqua-Vu cameras)in their ability to 1)facilitate detection of adults under potential cover items and 2)facilitate nest detection and monitoring in both wild and captive *** cameras were successful in detecting individual hellbender presence,albeit with different resolutions and detection *** borescope was better at accessing deep cavities given its large flexible attachment which allowed for greater flexibility of scanning crevices of adult ***,search time increased and even low levels of suspended sediment reduced visibility with the *** Aqua-Vu camera provided greater overall visibility and faster detection of individuals under both natural and artificial *** was a significant difference in the amount of time required to detect hellbenders with each camera design when searching under natural rocks(borescope:median=67.8 s,Aqua-Vu:median=39.1 s;Kruskal Wallis Test H=15.62,pl shelters(borescope:median=30.9 s,A

关键词： Captive propagation Hellbender Aquatic science Freshwater conservation Underwater camera systems

low prevalence of germline hMSH6 mutations in colorectal cancer families from Spain

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World Journal of Gastroenterology 2005年第37期11卷 5770-5776页

作者： Ana Sánchez de Abajo Miguel de la Hoya Alicia Tosar Javier Godino Juan Manuel Fernández Jose lopez Asenjo Beatriz Perez Villamil Pedro Perez Segura Eduardo diaz-Rubio Trinidad Caldes laboratorio de Oncología Molecular Hospital Clinico San Carlos Madrid Spain Servicio de Anatomía Patológica Hospital Clinico San Carlos Madrid Spain Servicio de Oncología Médica Hospital Clinico San Carlos Madrid Spain

AIM: To investigate the prevalence and penetrance of hMSH6 mutations in Spanish HNPCC families that was negative for mutation in hMlH1 or ***: We used PCR-based DGGE assay and direct Sequencing to screen for hMSH6 gene in 91 HNPCC ***: we have identified 10 families with germ-line mutations in the DNA sequence. These mutations included two intronic variation, three missense mutation, one nonsense mutation, and four silent mutations. Among the 10 germ-line mutations identified in the Spanish cohort,8 were novel, perhaps, suggesting different mutational spectra in the Spanish population. Detailed pedigrees were constructed for the three families with a possible pathogenic hMSH6 mutation. The two silent mutations H388H and l758l, detected in a person affected of colorectal cancer at age 29, produce loss of the wild-type allele in the tumor sample. Immunohistochemical analysis showed that expression of MSH6 protein was lost only in the tumors from the carriers of V878A and Q263X ***: Altogether, our results indicate that disease-causing germ-line mutations of hMSH6 are very less frequent in Spanish HNPCC families.

关键词： HNPCC MMR hMSH6 MSI IHC SPANISH

小切口椎板间插装融合技术治疗腰椎管狭窄症的近期疗效

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中国修复重建外科杂志 2012年第6期26卷 703-707页

作者：王少飞晁建虎卫建民 Fernando G.diaz 宝鸡市中医医院骨科陕西宝鸡721003 美国密歇根州奥克兰大学威廉.博蒙特医院神经外科

目的探讨经小切口局部椎板减压,椎板间插装融合(interlaminar lumbar instrumented fusion,IlIF)技术治疗腰椎管狭窄症的近期疗效。方法 2009年11月-2011年1月,采用经小切口椎板减压、IlIF治疗16例腰椎管狭窄症患者。男7例,女9例;年龄49... 详细信息

目的探讨经小切口局部椎板减压,椎板间插装融合(interlaminar lumbar instrumented fusion,IlIF)技术治疗腰椎管狭窄症的近期疗效。方法 2009年11月-2011年1月,采用经小切口椎板减压、IlIF治疗16例腰椎管狭窄症患者。男7例,女9例;年龄49～67岁,平均52.8岁。病程2年～9年4个月,平均4年7个月。16例均为退变性狭窄,4例合并双侧侧隐窝狭窄,3例合并腰椎间盘突出。病变节段:l3、42例,l4、54例,l5、S14例,l3、4和l4、5双节段2例,l4、5和l5、S1双节段4例。手术前后采用疼痛视觉模拟评分(VAS)、Oswestry功能障碍指数(ODI)进行临床疗效评定;CT扫描并测量椎管横截面面积。结果手术时间35～80 min,平均47 min;术中出血量120～350 ml,平均145 ml;住院时间4～15 d,平均7.8 d。除1例发生脑脊液漏,其余切口均Ⅰ期愈合。16例均获随访,随访时间12～22个月,平均14.8个月。术后CT扫描固定节段,示14例棘突间融合,2例可能融合;融合时间3～10个月,平均4.6个月。术后VAS评分、ODI及椎管横截面面积均较术前显著改善,差异有统计学意义(PlIF可兼顾椎板减压和重建后柱稳定性,同时保护脊髓,具有创伤小及操作简便的优点。

关键词：腰椎管狭窄症椎板减压椎板间插装融合