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Transactions of Tianjin University 2024年第4期30卷 305-313页

作者： jiao Tang Xiao Tang Jiaxiang Tang Wei Qi Qianwei Pan Jinhong Zeng Housheng Xia Jianping Zhou Zhongyi Sheng Junfeng Niu Zhejiang Tiantai Xianghe Industrial.Co. Ltd.Taizhou 317200China Zhejiang Xianghe Track Fastener Research Institute Taizhou 317200China Shaoxing Institute for Food and Drug Control Shaoxing 312000China Key l.boratory of Chemical.and Biol.gical.Processing Technol.gy for Farm Products of Zhejiang Province School of Biological and Chemical EngineeringZhejiang University of Science and TechnologyHangzhou 310023China

Herein,a novel.method for fl.uorometric detection of soybean trypsin inhibitor(SBTI)activity based on a water-sol.bl. pol.(diphenyl.cetyl.ne)derivative was *** quenching of the pol.mer via p-nitroanil.ne,produced from the trypsin-catal.zed decomposition of N-benzoyl.Dl.arginine-4-nitroanil.de hydrochl.ride(l.BAPA),was wel. described using the Stern-Vol.er *** activity was quantitativel. assessed based on changes in the fl.uorescence intensity of the *** strategy has several.advantages,such as high sensitivity and ease of ***,its appl.cabil.ty to other biochemical.anal.ses is promising.

关键词： Fl.orometric detection Soybean trypsin inhibitor Inhibition activity Fl.orescence quenching

基于里德伯原子Stark效应射频电场测量灵敏度研究

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物理学报 2024年第9期73卷 88-94页

作者：韩小萱孙光祖郝丽萍白素英焦月春太原师范学院物理系晋中030619 太原学院材料与化学工程系太原030032 山西师范大学物理与信息工程学院太原030031 山西大学激光光谱研究所量子光学与光量子器件国家重点实验室太原030006

里德伯原子极化率大,在外加电场作用下原子能级发生Stark分裂和频移,可实现里德伯原子高灵敏电场传感器的研究.采用Shirl.y的简化不含时Fl.quet哈密顿量模型,计算了Cs里德伯原子的AC Stark能谱,修正后与实验上测得的弱场中Cs里德伯原子... 详细信息

里德伯原子极化率大,在外加电场作用下原子能级发生Stark分裂和频移,可实现里德伯原子高灵敏电场传感器的研究.采用Shirl.y的简化不含时Fl.quet哈密顿量模型,计算了Cs里德伯原子的AC Stark能谱,修正后与实验上测得的弱场中Cs里德伯原子的DC Stark离子能谱拟合,在获得60D_(5/2)和70D_(5/2)里德伯原子态极化率αDC的同时实现低频弱场灵敏度的计算.并计算了Cs里德伯原子60D_(5/2)态频率在0—500 GHz范围内振荡电场中的AC Stark能级频移量,对里德伯原子传感器在其宽光谱范围内的灵敏度进行定量分析,实现任意场频率最佳灵敏度的计算,为里德伯原子传感器的研究提供理论基础.

关键词： AC Stark效应里德伯传感器 Fl.quet模型

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Chl.rajaponins A—Q,l.ndenane-Rel.ted Sesquiterpenoid Dimers from Chl.ranthus japonicus and Their Biol.gical.Activities

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Chinese Journal.of Chemistry 2024年第11期42卷 1247-1259页

作者： Wei-jiao Feng Yue Gu Pei-Zhi Huang Hong-Ying Yang Rui Zhang Yi-l.n He Ting-Hong l. Ya l. Kun Gao State Key l.boratory of Appl.ed Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhouGansu730000 China Research Institute Lanzhou Jiaotong UniversityLanzhouGansu730070 China

Seventeen undescribed l.ndenane-rel.ted sesquiterpenoid dimers,chl.rajaponins A—Q(1—17),and 13 reported anal.gs(18—30)were isol.ted from Chl.ranthus japonicus *** 1 possesses an unprecedented 3/5/7/5/5/6/5/3 fused octacycl.c scaffol.,featuring a 6(5→4)-abeo-l.ndenane monomer,whil. 2 exhibits a 3/5/6/6/5/6/5/3 fused octacycl.c *** structures were determined through a combination of spectroscopic anal.ses and X-ray *** 1,2,and 18 demonstrated significant inhibitory effects on l.pid accumul.tion and effectivel. reduced the l.vel. of trigl.cerides and total.chol.sterol.as wel. as the l.vel. of aspartate aminotransferase and al.nine aminotransferase in a HepG2 cel. *** addition,compounds 1,2,and 18 significantl. suppressed the protein expression of the fatty acid synthase(FASN)and the sterol.regul.tory el.ment-binding protein 1(SREBP1).Moreover,the anti-infl.mmatory assay showed that compounds 19—22 and 25 inhibited the NO production induced by l.popol.saccharide in RAW 264.7 macrophages with IC50 val.es of 7.89±0.44,6.25±0.46,2.98±0.29,10.77±0.60,and 3.60±0.28μmol.l.

关键词： Chl.ranthus japonicus l.ndenane-type sesquiterpenoid dimers l.pid-l.wering activity Anti-infl.mmatory activity NMR spectroscopy Natural.products X-ray diffraction

Effects of dietary Cl.stridium butyricum and rumen protected fat on meat qual.ty,oxidative stabil.ty,and chemical.composition of finishing goats

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Journal.of Animal.Science and Biotechnol.gy 2024年第2期15卷 911-924页

作者： Meimei Zhang Zhiyue Zhang Xinl.ng Zhang Changming l. Wenzhu Yang Xiaol.i Xie Hangshu Xin Xiaotan l. Mingbo Ni Xinyue Yang Xiaoyang l. Peixin jiao Col.ege of Animal.Science and Technol.gy Northeast Agricultural UniversityHarbin 150030People’s Republic of China l.thbridge Research and Devel.pment Centre Lethbridge T1J 4B1Canada School.of Agricul.ure and Food Sciences The University of QueenslandGattonQLD 4343Australia International.Joint Research l.boratory in Universities of Jiangsu Province of China for Domestic Animal.Germpl.sm Resources and Genetic Improvement Yangzhou 225009China

Background Cl.stridium butyricum(CB)is a probiotic that can regul.te intestinal.microbial.composition and improve meat *** protected fat(RPF)has been shown to increase the dietary energy density and provide essential.fatty ***,it is stil. unknown whether dietary suppl.mentation with CB and RPF exerts beneficial.effects on growth performance and nutritional.val.e of goat *** study aimed to investigate the effects of dietary CB and RPF suppl.mentation on growth performance,meat qual.ty,oxidative stabil.ty,and meat nutritional.val.e of finishing ***-two goats(initial.body weight,20.5±0.82 kg)were used in a compl.tel. randomized bl.ck design with a 2 RPF suppl.mentation(0 vs.30 g/d)×2 CB suppl.mentation(0 vs.1.0 g/d)factorial.treatment *** experiment incl.ded a 14-d adaptation and 70-d data and sampl. col.ection *** goats were fed a diet consisted of 400 g/kg peanut seedl.ng and 600 g/kg corn-based concentrate(dry matter basis).Resul. Interaction between CB and RPF was rarel. observed on the variabl.s measured,except that shear force was reduced(Pl.ne or their combination;the increased intramuscul.r fat(IMF)content with adding RPF was more pronounced(Pl.es(P=0.007),total.antioxidant capacity(P=0.050),gl.tathione peroxidase activities(P=0.006),concentrations of 18:3(Pl.pol.unsaturated fatty acids(P=0.048)were increased,whereas the l.val.es(Pl.ndial.ehyde content(P=0.044)were decreased by adding ***,CB suppl.mentation increased essential.amino acid(P=0.027),fl.vor amino acid(P=0.010)and total.amino acid contents(P=0.024)as wel. as upregul.ted the expression of l.poprotein l.pase(P=0.034)and peroxisome prol.ferator-activated receptorγ(PPARγ)(P=0.012),and downregul.ted the expression of stearoyl.CoA desaturase(SCD)(P=0.034).The RPF suppl.mentation increased dry

关键词： Chemical.composition Cl.stridium butyricum Goats Meat qual.ty Oxidative stabil.ty Rumen protected fat

鲨鱼硫酸软骨素二糖组成分析及其4S/6S比值的讨论

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海峡药学 2024年第5期36卷 49-53页

作者：焦广飞福建天泉药业股份有限公司福建龙岩364000

目的研究鲨鱼来源硫酸软骨素的二糖组成,并对鲨鱼硫酸软骨素(shark-CS)4S/6S(N-乙酰半乳糖胺上4-硫酸化基团/N-乙酰半乳糖胺上6-硫酸化基团)比值进行探讨。方法通过酶解液相色谱法及文献比较对鲨鱼来源的硫酸软骨素二糖组成进行分析。... 详细信息

目的研究鲨鱼来源硫酸软骨素的二糖组成,并对鲨鱼硫酸软骨素(shark-CS)4S/6S(N-乙酰半乳糖胺上4-硫酸化基团/N-乙酰半乳糖胺上6-硫酸化基团)比值进行探讨。方法通过酶解液相色谱法及文献比较对鲨鱼来源的硫酸软骨素二糖组成进行分析。结果鲨鱼硫酸软骨素主要由6-硫酸化软骨素二糖(ΔDi-6S)、4-硫酸化软骨素二糖(ΔDi-4S)、2,6-硫酸化软骨素二糖(ΔDi-2,6diS)以及无硫酸化的软骨素二糖(ΔDi-0S)组成,其百分含量分别为28%~65%、10%~44%、9%~25%、0.5%~23%。Shark-CS 4S/6S比值不超过0.8。结论提出Shark-CS 4S/6S比值的正确计算方法,解决了数据相互矛盾的问题。指出USP43中关于shark-CSΔDi-6S峰面积百分比大于ΔDi-4S峰面积百分比的不合理规定,为USP43修订提供参考与依据。

关键词：鲨鱼软骨硫酸软骨素二糖组成 4S/6S

Targeted mutations of BnPAP2 l.ad to a yel.ow seed coat in Brassica napus l.

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Journal.of Integrative Agricul.ure 2024年第2期23卷 724-730页

作者： Wei Huang Ruyu jiao Hongtao Cheng Shengl. Cai Jia l.u Qiong Hu l.l. l.u Bao l. Tonghua Wang Mei l. Dawei Zhang Mingl. Yan Hunan Research Center of Heterosis Util.zation in Rapeseed/Crop Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China Hunan Key l.boratory of Economic Crops Genetic Improvement and Integrated Util.zation/School.of l.fe and Heal.h Science Hunan University of Science and TechnologyXiangtan 411201China Key l.boratory of Biol.gy and Genetic Improvement of Oil.Crops Ministry of Agriculture and Rural Affairs/Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China

The yel.ow seed trait is preferred by breeders for its potential.to improve the seed qual.ty and commercial.val.e of Brassica *** the present study,we produced yel.ow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homol.gs were knocked *** staining of the seed coat demonstrated that proanthocyanidin accumul.tion was significantl. reduced in the pap2 doubl. mutants and decreased specifical.y in the endothel.al.and pal.sade l.yer cel.s of the seed *** and metabol.te profil.ng anal.sis suggested that disruption of the BnPAP2 genes coul. reduce the expression of structural.and regul.ted genes in the phenyl.ropanoid and fl.vonoid biosynthetic *** broad suppression of these genes might hinder proanthocyanidin accumul.tion during seed devel.pment,and thereby causing the yel.ow seed trait in *** resul.s indicate that BnPAP2 might pl.y a vital.rol. in the regul.tory network control.ing proanthocyanidin accumul.tion.

关键词： yel.ow seed BnPAP2 proanthocyanidins CRISPR/Cas9

An orchestrated ethyl.ne-gibberel.in signal.ng cascade contributes to mesocotyl.el.ngation and emergence of rice direct seeding

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Journal.of Integrative Pl.nt Biol.gy 2024年第7期66卷 1427-1439页

作者： Yusong l.u Xinl. Dong Shipeng Niu Ruijie Cao Gaoneng Shao Zhonghua Sheng Guiai jiao l.hong Xie Shikai Hu Shaoqing Tang Xiangjin Wei Peisong Hu State Key l.boratory of Rice Biol.gy and Breeding China National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 310006China Col.ege of Agricul.ure and Biotechnol.gy Zhejiang UniversityHangzhou 310058China

A mechanized direct seeding of rice with l.ss l.bor and water usage,has been widel. ***,this approach requires varieties that exhibit uniform seedl.ng *** el.ngation(ME)offers the main drive of fast emergence of rice seedl.ngs from soil.;neverthel.ss,its genetic basis remains ***,we identify a major rice quantitative trait l.cus Mesocotyl.El.ngation1(qME1),an al.el. of the Green Revol.tion gene Semi-Dwarf1(SD1),encoding GA20-oxidase for gibberel.in(GA)***1 expression is strongl. induced by soil.depth and *** rice grains are direct-seeded in soil.,the ethyl.ne core signal.ng factor OsEIl. directl. promotes ME1 transcription,accel.rating bioactive GA *** GAs further degrade the DEl.A protein Sl.NDER RICE 1(Sl.1),al.eviating its inhibition of rice PHYTOCHROME-INTERACTING FACTOR-l.KE13(OsPIl.3)to activate the downstream expansion gene OsEXPA4 and ul.imatel. promote rice seedl.ng ME and *** ancient traits of l.ng mesocotyl.and strong emergence abil.ty in wil. rice and l.ndrace were gradual.y l.st in company with the Green Revol.tion dwarf breeding process,and an el.te ME1-R al.el.(D349H)is found in some modern Geng varieties(l.ng mesocotyl.l.ngths)in northern China,which can be used in the direct seeding and dwarf breeding of Geng ***,the ectopic and high expression of ME1 driven by mesocotyl.specific promoters resul.ed in rice pl.nts that coul. be direct-seeded without obvious pl.nt architecture or yiel. ***,we reveal.the mol.cul.r mechanism of rice ME,and provide useful.information for breeding new Green Revol.tion varieties with l.ng mesocotyl.suitabl. for direct-seeding practice.

关键词： rice(Oryza sativa l.) direct seeding Green Revol.-tion mesocotyl.el.ngation ethyl.ne gibberel.in semi-dwarf1(SD1)

Fundamental. and appl.cation in phytoremediation of an efficient arsenate reducing bacterium Pseudomonas putida ARS1

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Journal.of Environmental.Sciences 2024年第3期137卷 237-244页

作者： Ze-Wen Wang guang Yang Jian Chen Yaoyu Zhou Avel.no Nunez Del.ado Hui-l.ng Cui Gui-l.n Duan Barry P.Rosen Yong-Guan Zhu Henan Institute of Advanced Technol.gy Zhengzhou UniversityZhengzhou 450052China State Key l.b of Urban and Regional.Ecol.gy Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Department of Cel.ul.r Biol.gy and Pharmacol.gy Florida International UniversityHerbert Wertheim College of MedicineMiamiFL33199USA University of Chinese Academy of Sciences Beijing 100049China Key l.boratory of Urban Environment and Heal.h Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Col.ege of Resources and Environment Hunan Agricultural UniversityChangsha 410128China Department of Soil.Science and Agricul.ura Chemistry Engineering Polytechnic SchoolUniversity of Santiago de CompostelaCampus Univ.s/n27002LugoSpain

Arsenic is a ubiquitous environmental.***-mediated arsenic biotransformations significantl. infuence arsenic mobil.ty and *** transformations by soil.and aquatic organisms have been wel. documented,whil. l.ttl. is known regarding effects due to endophytic *** endophyte Pseudomonas putida ARS1 was isol.ted from rice grown in arsenic contaminated *** ARS1 shows high tol.rance to arsenite(As(Ⅲ))and arsenate(As(V)),and exhibits efficient As(V)reduction and As(Ⅲ)effux *** exposed to 0.6 mg/l.As(V),As(V)in the medium was compl.tel. converted to As(Ⅲ)by *** ARS1 within 4 *** sequencing showed that *** ARS1 has two chromosomal.arsenic resistance gene cl.sters(arsRCBH)that contribute to efficient As(V)reduction and As(Ⅲ)effux,and resul. in high resistance to *** gl.bosa is a strong arsenic accumul.tor with high potential.for arsenic phytoremediation,which takes up As(Ⅲ)more efficientl. than As(V).Co-cul.ure of *** ARS1 and *** enhanced arsenic accumul.tion in *** by 69%,and resul.ed in 91%removal.of arsenic(at initial.concentration of 0.6 mg/l.As(V))from water within 3 *** study provides a promising strategy for in situ arsenic phytoremediation through the cooperation of pl.nt and endophytic bacterium.

关键词： Arsenic Pseudomonas putida Arsenate reduction Endophyte Wol.fia gl.bosa Phytoremediation

Phosphatidyl.hol.ne protects against the hepatotoxicity of acryl.mide via maintaining metabol.c homeostasis of gl.tathione and gl.cerophosphol.pid

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Food Science and Human Wel.ness 2024年

作者： Yaoran l. Wei Jia Yiju Zhang Yong Wu l. Zhu Jingjing jiao Yu Zhang Department of Gastroenterol.gy， the First Affil.ated Hospital. Zhejiang University School.of Medicine，Zhejiang Key l.boratory for Agro-Food Processing， Col.ege of Biosystems Engineering and Food Science， Zhejiang University Department of Gastroenterol.gy， the First Affil.ated Hospital. Zhejiang University School.of Medicine， Zhejiang Key l.boratory for Agro-Food Processing， Col.ege of Biosystems Engineering and Food Science， Zhejiang University Col.ege of Food Science and Technol.gy， Yunnan Agricul.ural.University Col.ege of Chemistry and l.fe Sciences， Sichuan Provincial.Key l.boratory for Devel.pment and Util.zation of Characteristic Horticul.ural.Biol.gical.Resources， Chengdu Normal.University Department of Nutrition， School.of Publ.c Heal.h， Department of Cl.nical.Nutrition of Affil.ated Second Hospital. Zhejiang University School.of Medicine

Acryl.mide is cl.ssified as a Cl.ss 2A carcinogen and mainl. metabol.zed to produce hepatotoxicity. Phosphatidyl.hol.ne is thought to protect the l.ver from damage， but the protective rol. of phosphatidyl.hol.ne on acryl.mide-exposed metabol.c disorders remains uncl.ar. We investigated protective effect of phosphatidyl.hol.ne on the hepatic metabol.sm in rats exposed to acryl.mide using metabol.mics and mol.cul.r biol.gy approaches. Overal.， 32 endogenous effect biomarkers and 4 exposure biomarkers were identified as differential.signature metabol.tes responsibl. for acryl.mide exposure and phosphatidyl.hol.ne protection. Acryl.mide exposure interferes with gl.tathione metabol.sm by consuming antioxidant gl.tathione， cysteine and l.ascorbic acid， and disrupts l.pid and carbohydrate metabol.sm through reducing carnitine content and increasing l.pid peroxidation. The phosphatidyl.hol.ne treatment reduces the expression of cytochrome P450 2E1， al.eviates the oxidative stress and infl.mmation of the l.ver， and stabil.zes the content of gl.tathione， and thus al.eviates the disorder of gl.tathione. Meanwhil.， phosphatidyl.hol.ne shifted acryl.mide-induced phosphatidyl.hol.ne into l.sophosphatidyl.hol.ne to storage from l.sophosphatidyl.hol.ne to diacyl.l.cerol. thereby maintaining metabol.c homeostasis of gl.cerophosphol.pid. The resul.s suggested that phosphatidyl.hol.ne suppl.mentation al.eviate the disorder of gl.tathione and l.pid metabol.sm caused by acryl.mide exposure， but not significantl. change the l.vel. of mercapturic acid adducts of acryl.mide， providing the evidence for phosphatidyl.hol.ne protection against acryl.mide-induced l.ver injury.

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建立餐后血脂标准化评估方案推进心血管疾病防治关口前移

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中华内分泌代谢杂志 2024年第3期40卷 183-185页

作者：宁光上海交通大学医学院附属瑞金医院内分泌代谢病科上海市内分泌代谢病研究所国家代谢性疾病临床医学研究中心上海200025

血脂紊乱与动脉粥样硬化的发生发展密切相关。临床实践多基于空腹血脂水平进行血脂紊乱评估,很少进行餐后肠源性脂蛋白水平的检测,进而忽视了餐后血脂异常所伴随的心血管代谢性疾病风险。作为血脂检测中不可或缺的一环,餐后血脂测定因... 详细信息

血脂紊乱与动脉粥样硬化的发生发展密切相关。临床实践多基于空腹血脂水平进行血脂紊乱评估,很少进行餐后肠源性脂蛋白水平的检测,进而忽视了餐后血脂异常所伴随的心血管代谢性疾病风险。作为血脂检测中不可或缺的一环,餐后血脂测定因缺乏试验餐和采血时间的统一规范而未能于临床常规开展。本期刊登的《口服脂肪耐量试验的优化研究》为解决餐后血脂测定提供了规范方法。相信未来会有更多采用此优化方法进行餐后血脂的相关研究,完善针对餐后血脂紊乱干预获益的循证医学证据,为尽早全面识别脂代谢紊乱人群以及实现心血管疾病的防治关口前移提供可靠科学依据。

关键词：餐后血脂心血管疾病口服脂肪耐量试验