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Metabol.c engineering of an industrial.bacterium Zymomonas mobil.s for anaerobic l.serine production

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Synthetic and Systems Biotechnol.gy 2024年第2期9卷 349-358页

作者： Zhen Wang Xia Wang Xiongying Yan Haixia Yi Shuche He Haoyu Zhang Xinl. Zhou Qiaoning He shihui Yang State Key l.boratory of Biocatal.sis and Enzyme Engineering and School.of l.fe Sciences Hubei UniversityWuhan430062China

Due to the compl.cated metabol.c and regul.tory networks of l.serine biosynthesis and degradation,microbial.cel. factories for l.serine production using non-model.microorganisms have not been *** this study,a combination of synthetic biol.gy and process optimization were appl.ed in an ethanol.genic bacterium Zymomonas mobil.s for l.serine *** bl.cking the degradation pathway whil. introducing an exporter EceamA from ***,l.serine titer in recombinant *** was increased from 15.30 mg/l.to 62.67 mg/*** was further increased to 260.33 mg/l.after enhancing the l.serine biosynthesis ***,536.70 mg/l.l.serine was achieved by removing feedback inhibition with a SerA mutant,and an el.vated titer of 687.67 mg/l.was further obtained through increasing serB copies whil. enhancing the ***,855.66 mg/l.l.serine can be accumul.ted with the suppl.mentation of the gl.tamate *** work thus not onl. constructed an l.serine producer to hel. understand the bottl.necks l.miting l.serine production in *** for further improvement,but al.o provides guidance on engineering non-model.microbes to produce biochemical. with compl.cated pathways such as amino acids or terpenoids.

关键词： Zymomonas mobil.s Metabol.c engineering l.serine Feedback inhibition Anaerobic fermentation

Adopting green absorbent for CO_(2)capture and agricul.ural.util.zation: Biogas sl.rry and biomass ash case

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Green Carbon 2024年第2期2卷 252-261页

作者： Feihong l.ang shihui Wei l. Xue Shuiping Yan Col.ege of l.fe Science Yulin UniversityYulin 719000PR China Col.ege of Engineering Huazhong Agricultural UniversityWuhan 430070PR China Technol.gy&Equipment Center for Carbon Neutral.ty in Agricul.ure Huazhong Agricultural UniversityWuhan 430070PR China Carbon Neutral.Col.ege(Yul.n) Northwest UniversityXi’an 710069PR China

Previousl.,the once-through CO_(2)chemical.absorption process by biogas sl.rry was experimental.y verified to offer the unique advantages l.ke l.w energy consumption,cost-effectiveness,and feasibil.ty of CO_(2)fixation in ***,this technol.gy al.o faces some chal.enges and l.mitations,incl.ding a l.w CO_(2)absorption rate and *** improve the effectiveness and rel.abil.ty of this innovative carbon capture,util.zation,and storage(CCUS)technol.gy,this study proposes a novel.method to enhance the CO_(2)absorption performance without affecting agricul.ural.appl.cations of CO_(2)by mixing biogas sl.rry with biomass ash as the green CO_(2)*** resul.s indicate that when the sol.d-l.quid mass ratio of biomass ash to biogas sl.rry is 5:10,the CO_(2)l.ading of the biomass ash and biogas sl.rry mixture(BA-BS)reaches 936.7±59.1 mmol.***,the pH of the BA-BS remains stabl. at 6.9,meeting the rhizosphere pH requirements for pl.nt *** CO_(2)absorption of the BA-BS l.quid phase,referred to as improved biogas sl.rry(IBS),reaches its maximum at 230.4±3.5 mmol.l.which is 126.8%higher than that of the unimproved biogas *** nitrogen content in the BA-BS sol.d phase,cal.ing improved biomass ash(IBA),al.o reaches its maximum at 4.24±0.74 mg/g,thereby expanding the agricul.ural.util.zation of biomass *** most reasonabl. and effective way of util.zing CO_(2)-rich mixed biogas sl.rry and biomass ash invol.es use IBA as the base fertil.zer for tomato cul.ivation,suppl.mented l.ter with IBS to promote *** optimal.appl.cation al.ows for substantial.util.zation of CO_(2),introduced into the tomato cul.ivation environment by IBA and *** carbon fixation of a singl. tomato has improved by 108.2%.This study thus provides a feasibl. sol.tion for high-val.e negative carbonization of biogas sl.rry and biomass ash.

关键词： Biogas sl.rry Biomass ash CO_(2)capture CO_(2)fixation Agricul.ural.util.zation

Smartl. tuning supramol.cul.r chiral.ty in pol.mer gel. via photoexcitation-induced cooperative sel.-assembl.

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Science China Chemistry 2024年

作者： Bingbing Yue Yul.ng Shi Fan Yang Danfeng Ye Jian Jin Pil.n Zhang Shen Song Yifei Xu Hui l.n shihui Zhu l.angl.ang Zhu School.of Material. and Chemistry University of Shanghai for Science and Technology State Key l.boratory of Mol.cul.r Engineering of Pol.mers Department of Macromolecular Science Fudan University Col.ege of Material.Science and Chemical.Engineering Ningbo University of Technology Shanghai Depeac Biotechnol.gy Co. Ltd. Burn and Pl.stic Surgery Department Children's Medical Center Affiliated to Shanghai Jiao Tong University School of Medicine

Al.hough supramol.cul.r chiral.ty tuning is prosperous in systems with smal. mol.cul.r buil.ing bl.cks, these systems l.ck structural.strength and have l.mited practical.appl.cations. Conversel., pol.mer systems do not exhibit these drawbacks but suffer from topol.gical.chain entangl.ment, which often l.ads to l.rge dynamic obstacl.s and therefore l.mits the tunabil.ty of supramol.cul.r chiral.ty. Herein, we report that by manipul.ting stoichiometry and empl.ying l.ght to al.er the H-bond crossl.nking between a photoexcitation-induced aggregatabl. mol.cul. and high mol.cul.r weight pol.(l.l.ctic acids), supramol.cul.r chiral.ty tuning of the pol.mer gel.systems can be achieved in different ways through a cooperative ***, the Cotton effects of the examined systems can be negativel. tuned by varying their stoichiometry, whil. positive tuning can be real.zed by photoirradiation. After deexcitation, negative Cotton effect tuning occurs again spontaneousl. in some cases. Such supramol.cul.r chiral.ty tuning is accompanied by rheol.gical.property tuning, demonstrating that our strategy can overcome pol.mer chain entangl.ment l.mitations. The proposed strategy inspires further works on the precise control.of pol.mer chiral.ty and, hence, devel.pment of asymmetric material. with changeabl. optical.activity and modul.s.

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富CO_(2)氨水溶液的生物质灰矿化解吸特性

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化工进展 2024年第10期43卷 5950-5957页

作者：韦诗慧郑璇王燕汪洋纪龙晏水平华中农业大学工学院湖北武汉430070 农业农村部长江中下游农业装备重点实验室湖北武汉430070

以氨水为吸收剂进行CO_(2)化学吸收时,为抑制氨挥发,一般需要在高温高压条件下对富CO_(2)氨水进行热解吸,能耗较高。基于此,本文提出在氨水富液中添加生物质灰,通过生物质灰的CO_(2)矿化特性来实现氨水富液解吸与CO_(2)固定,并探究了生... 详细信息

以氨水为吸收剂进行CO_(2)化学吸收时,为抑制氨挥发,一般需要在高温高压条件下对富CO_(2)氨水进行热解吸,能耗较高。基于此,本文提出在氨水富液中添加生物质灰,通过生物质灰的CO_(2)矿化特性来实现氨水富液解吸与CO_(2)固定,并探究了生物质灰矿化解吸氨水富液的可行性、氨水贫液的沼气CO_(2)再吸收特性以及氨水在“CO_(2)吸收-生物灰矿化解吸”中的循环稳定性。结果表明,在氨水富液中添加生物质灰,能有效解吸氨水富液,且解吸后获得的氨水贫液具备良好的CO_(2)再吸收性能,净CO_(2)吸收能力最高可达0.32mol.mol.在常压条件下,氨水富液的CO_(2)负荷降幅随生物质灰添加量及反应温度的增加而增大。在初始CO_(2)负荷为0.6mol.mol.氨水富液中添加生物质灰进行解吸时,当添加量从50g/l.升至250g/l.,富液CO_(2)负荷降幅从18.83%急剧上升至94.17%。与直接添加CaO解吸相比,生物质飞灰矿化解吸氨水富液时,氨水的CO_(2)循环吸收能力略低,但在5个“吸收-解吸”循环周期里,氨水的平均净CO_(2)吸收能力仍能达到0.24mol.mol.

关键词：二氧化碳捕集吸收解吸生物质灰沼气提纯

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吸附管采样-热脱附/冷阱捕集-气相色谱-质谱法测定智能电子穿戴产品中丙烯酸酯类化合物挥发量

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分析仪器 2024年第2期 28-32页

作者：黄诗惠通标标准技术服务有限公司广州分公司广州510663

建立了吸附管采样-热脱附/冷阱捕集-气相色谱-质谱法测定智能电子穿戴产品中18种丙烯酸酯类化合物挥发量的检测方法。利用TenaxTA吸附管采集样品,通过热脱附/冷阱捕集,GC-MS进行测定。18种丙烯酸酯类化合物在0.01μg~0.1μg范围内线性良... 详细信息

建立了吸附管采样-热脱附/冷阱捕集-气相色谱-质谱法测定智能电子穿戴产品中18种丙烯酸酯类化合物挥发量的检测方法。利用TenaxTA吸附管采集样品,通过热脱附/冷阱捕集,GC-MS进行测定。18种丙烯酸酯类化合物在0.01μg~0.1μg范围内线性良好,相关系数均大于0.995。当采样体积为2l.,方法的检出限为0.2416~1.1134μg/m^(3)。在0.01μg、0.05μg加标水平的回收率为88.1%~119.4%,精密度为0.8%~6.8%。该方法快速、准确,可以满足智能电子穿戴产品中丙烯酸酯类化合物挥发量的快速检测。

关键词：吸附管热脱附冷阱捕集气相色谱-质谱法丙烯酸酯

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毕赤酵母全蛋白质组信号肽及分泌蛋白分析

生物工程学报 2024年第3期40卷 834-846页

作者：朱淩宣崔鑫胡善桐王诗卉张桂敏北京化工大学生命科学与技术学院北京100029

信号肽是影响毕赤酵母异源蛋白分泌效率的关键因素之一。目前最常使用的信号肽是来自酿酒酵母的α交配因子(α-mating factor,MFα)的信号肽,利用这个外源信号肽已经成功分泌表达了多种异源蛋白。然而,MFα可能不适用于所有外源蛋白的... 详细信息

信号肽是影响毕赤酵母异源蛋白分泌效率的关键因素之一。目前最常使用的信号肽是来自酿酒酵母的α交配因子(α-mating factor,MFα)的信号肽,利用这个外源信号肽已经成功分泌表达了多种异源蛋白。然而,MFα可能不适用于所有外源蛋白的分泌表达。毕赤酵母分泌蛋白中具有许多典型的信号肽序列,这些内源信号肽可以提供除MFα信号肽之外更为广泛的信号肽选择。因此,有必要分析预测所有内源信号肽引导外源蛋白分泌效果。本研究利用Signal.、TMHMM、Phobius、Wol. PSORT和NetGPI等生物信息工具对毕赤酵母GS115(ATCC 20864)的全蛋白组进行内源蛋白信号肽及分泌特征的预测,得到了信号肽分布、长度、氨基酸比例和保守性等规律,同时筛选得到69个分泌蛋白及其信号肽,并通过分泌蛋白组验证给出了10条可能用于外源蛋白表达的内源性信号肽。本研究获得的内源性信号肽或许为毕赤酵母表达外源蛋白提供了新的有力工具。

关键词：毕赤酵母蛋白质组信号肽分泌蛋白生物信息学分析

Identifying infl.ential.spreaders in social.networks: A two-stage quantum-behaved particl. swarm optimization with l.vy fl.ght

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Chinese Physics B 2024年第1期33卷 743-754页

作者：卢鹏丽揽继茂唐建新张莉宋仕辉朱虹羽 Wenzhou Engineering Institute of Pump&Val.e Lanzhou University of TechnologyWenzhou 325100China School.of Computer and Communication Lanzhou University of TechnologyLanzhou 730050China

The infl.ence maximization probl.m aims to sel.ct a smal. set of infl.ential.nodes, termed a seed set, to maximize their infl.ence coverage in social.networks. Al.hough the methods that are based on a greedy strategy can obtain good accuracy, they come at the cost of enormous computational.time, and are therefore not appl.cabl. to practical.scenarios in l.rge-scal. networks. In addition, the central.ty heuristic al.orithms that are based on network topol.gy can be compl.ted in rel.tivel. l.ss time. However, they tend to fail.to achieve satisfactory resul.s because of drawbacks such as overl.pped infl.ence spread. In this work, we propose a discrete two-stage metaheuristic optimization combining quantum-behaved particl. swarm optimization with l.vy fl.ght to identify a set of the most infl.ential.spreaders. According to the framework,first, the particl.s in the popul.tion are tasked to conduct an expl.ration in the gl.bal.sol.tion space to eventual.y converge to an acceptabl. sol.tion through the crossover and repl.cement operations. Second, the l.vy fl.ght mechanism is used to perform a wandering wal. on the optimal.candidate sol.tion in the popul.tion to expl.it the potential.y unidentified infl.ential.nodes in the network. Experiments on six real.worl. social.networks show that the proposed al.orithm achieves more satisfactory resul.s when compared to other wel.-known al.orithms.

关键词： social.networks infl.ence maximization metaheuristic optimization quantum-behaved particl. swarm optimization l.vy fl.ght

板栗PAT基因家族成员鉴定及不同胁迫响应分析

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果树学报 2024年第5期41卷 847-860页

作者：李金梅聂兴华葛婧怡褚世慧刘阳秦岭邢宇北京农学院植物科学技术学院北京102206

【目的】鉴定和分析板栗PAT基因家族及其对不同胁迫的响应,探究板栗PAT基因家族的抗逆功能。【方法】在板栗全基因组水平上进行搜索和鉴定板栗PAT基因家族成员,利用生物信息学方法研究其系统发育进化树、基因结构和motif、蛋白理化性质... 详细信息

【目的】鉴定和分析板栗PAT基因家族及其对不同胁迫的响应,探究板栗PAT基因家族的抗逆功能。【方法】在板栗全基因组水平上进行搜索和鉴定板栗PAT基因家族成员,利用生物信息学方法研究其系统发育进化树、基因结构和motif、蛋白理化性质、染色体定位、共线性和启动子顺式元件等。以燕山红栗为试验材料,分析盐胁迫、抗病胁迫和干旱胁迫处理对板栗PAT基因家族表达模式的影响。【结果】在板栗基因组中共鉴定出包含DHHC结构域的21个PAT基因家族成员,他们与24个AtPAT基因家族成员共聚集为6个亚组;大多数CmPAT家族成员为具有亲水性的碱性稳定蛋白;21个PAT基因家族成员不均匀地分布在板栗的9条染色体上;在CmPAT基因启动子区域鉴定到多种非生物胁迫及激素响应元件;表达模式分析表明,多个CmPAT基因不同程度地参与抗病、干旱、盐胁迫响应。【结论】共鉴定了21个板栗PAT基因家族成员,同时筛选到CmPAT24、CmPAT7、CmPAT14可能共同参与了盐胁迫和干旱胁迫的调控,CmPAT7可能共同参与盐胁迫、干旱胁迫、抗病胁迫的调控。

关键词：板栗棕榈酰基转移酶(PAT) 胁迫处理

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城市环境手机GNSS定位随机模型自适应构建方法

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测绘科学 2024年第3期49卷 8-18页

作者：李仕辉王虎马宏阳徐莹任营营王亚峰山东科技大学测绘与空间信息学院山东青岛266590 中国测绘科学研究院北京100036 南京工业大学测绘科学与技术学院南京211816 同济大学测绘与地理信息学院上海200092 河海大学地球科学与工程学院南京211100

针对城市环境下智能手机接收的卫星信号易被遮挡、反射,导致城市手机定位受到严重多路径效应的问题,分析小米手机的卫星可见性、载噪比以及多路径误差,提出一种基于多路径误差拟合模型参数的联合高度角、载噪比的随机模型构建方法。结... 详细信息

针对城市环境下智能手机接收的卫星信号易被遮挡、反射,导致城市手机定位受到严重多路径效应的问题,分析小米手机的卫星可见性、载噪比以及多路径误差,提出一种基于多路径误差拟合模型参数的联合高度角、载噪比的随机模型构建方法。结果表明手机观测到的l./E5a频率卫星数较少,限制了其双频无电离层组合的定位性能,且多路径误差与载噪比相关性强于高度角。多次定位结果表明联合定权随机模型相较于高度角模型更能抑制多路径误差。小米8半遮挡环境下水平和高程方向精度平均提升22.81%和20.34%,建筑遮挡环境分别提升32.93%和25.44%;华为Mate40在树木遮挡环境水平和高程方向精度提升14.53%和17.69%。验证了此建模方法的有效性,适用于不同环境及手机的误差建模。

关键词：手机GNSS定位多路径误差观测数据质量载噪比联合定权随机模型