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Microbial.Qual.ty and Mol.cul.r Identification of Enterotoxigenic Staphyl.coccus Strains Isol.ted from Dried, Smoked, and Braised Fish Sol. in ouagadougou Markets

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Advances in Microbiol.gy 2024年第1期14卷 59-76页

作者： Arouna ouédraogo Ganamé Abasse ouédraogo Henri Sidabéwindin ouédraogo François Tchoumbougnang Cheikna Zongo Al. Savadogo l.boratoire de Biochimie et d’Immunol.gie Appl.qué es (LABIA) Universit&eacute Joseph Ki-Zerbo Ouagadougou Burkina Faso l.boratoire de Val.risation et Contrô le de Qualit&eacute Institut des Sciences Halieutiques Universit&eacute de Douala Douala Cameroon

Background: The investigation of toxin genes in strains invol.ed in staphyl.coccal.food poisoning contributes to food safety. The aim of this study was to isol.te and identify enterotoxigenic Staphyl.coccus strains from dried, smoked, and braised fish sol. in ouagadougou markets. Methodol.gy: Staphyl.cocci were isol.ted using standard microbiol.gy methods. Staphyl.coccus strains were identified using API Staph kit (Reference # 20500, BioMerieux S.A., Marcy l.Etoil., France). The mol.cul.r identification of isol.ted Staphyl.coccus aureus strains was specifical.y confirmed by PCR using the Staur4 and Staur6 primers. The genes encoding enterotoxins, enterotoxin-l.ke toxins, exfol.ative toxins, and TSST-1 toxin were detected by mul.ipl.x PCR using specific primers from Inquaba Biotec West Africa l.d, Africa's Genomics Company. Resul.s: The resul.s of the microbiol.gical.qual.ty assessment indicated that most of the sampl.s anal.zed were found to be of unsatisfactory microbiol.gical.qual.ty according to the Staphyl.coccus aureus microbiol.gical.criteria (m = 102). Overal., onl. 12.55% of sampl.s were satisfactory, whil. 97.45% were unsatisfactory. The STAPH API gal.ery al.owed the identification of the fol.owing species: Staphyl.coccus aureus, Staphyl.coccus xyl.sus, Staphyl.coccus l.gdunensis, Staphyl.coccus hominis, Staphyl.coccus haemol.ticus, Staphyl.coccus l.ntus, Staphyl.coccus sciuri and Staphyl.coccus capitis. Of the 108 Staphyl.coccus isol.tes, 81 (75%) showed at l.ast one (1) toxin gene. Among the 21 toxin genes tested in this study, 20 genes were detected in al. strains anal.zed. The staphyl.coccal.toxin genes detected were present in both Staphyl.coccus aureus and the other coagul.se-negative strains isol.ted in this study. In addition, these genes are found individual.y or in association in certain strains. The most frequent genes detected in toxin gene-positive strains were: the tsst-1 gene in 45 isol.ted strains (41.7%), sei (16/14.8%), seg (13/12%), ser (

关键词： Fish Staphol.cuccus Toxin Genes ouagadougou

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Epidemiol.gical.Aspects of Cervicofacial.Cel.ul.tis Due to Dental.Origin in the City of ouagadougou (Burkina Faso)

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Open Journal.of Stomatol.gy 2021年第10期11卷 399-410页

作者： Wendpouiré Patrice l.urent Guiguimdé Kouamé Patrice Attogbain Jocel.ne V. W. Garé Yamsoul.ugri C. l. ouédraogo Mathieu Mil.ogo Tarcissus Konsem Training and Research Unit in Heal.h Sciences (UFR/SDS) Joseph Ki Zerbo University Ouagadougou Burkina Faso Department of Odontostomatol.gy and Maxil.ofacial.Surgery Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso Department of Surgery UFR of Odonto-Stomatology Félix Houphou&euml t-Boigny University of Abidjan Abidjan Republic of C&ocirc te d’Ivoire Department of Odontostomatol.gy and Maxil.ofacial.Surgery Bogodogo University Hospital Center Ouagadougou Burkina Faso

Background: Head and neck cel.ul.tis of dental.origin are pol.microbial.bacterial.infections invol.ing the cel.ul.-adipose spaces of the face and neck. The objective of this work was to describe the epidemiol.gical.and cl.nical.profil. of patients with head and neck cel.ul.tis. Methods: This was a cross-sectional.study conducted from 1 January to 30 June 2020. Al. patients consul.ing for cervico-facial.cel.ul.tis of dental.origin in 2 university hospital. in ouagadougou were incl.ded in the study. The usual.parameters of descriptive statistics were estimated for each variabl.. Resul.s: Two hundred and ninety-two cases (184 men and 108 women), aged 3 to 85 years were col.ected. The 25 - 35 years ol. were the most affected (33%). Decay was the l.ading dental.cause (95.6%). The del.y in consul.ation was incl.ded within 7 days in 74.66% of cases. Ninety-one-point forty-four percent of patients had poor oral.hygiene. Diffuse cel.ul.tis was the most common (64.04%) and peri-mandibul.r regions were the most invaded (42.81%). Concl.sion: The frequencies observed in our study al.ow us to concl.de that cervicofacial.cel.ul.tis of dental.origin is stil. frequent and affects a young and disadvantaged popul.tion.

关键词： Cervicofacial.Cel.ul.tis Odontogenic Infection Epidemiol.gical.and Cl.nical.Profil. ouagadougou

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Haematol.gical.and Biochemical.Variations in Cervico-Facial.Cel.ul.tis of Dental.Origin in the City of ouagadougou (Burkina Faso)

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Open Journal.of Stomatol.gy 2021年第10期11卷 387-398页

作者： Wendpouiré Patrice l.urent Guiguimdé Kouamé Patrice Attogbain Jocel.ne V. W. Garé Yamsoul.ugri C. l. ouédraogo Soul.ymane Bougoum Mathieu Mil.ogo Tarcissus Konsem Training and Research Unit in Heal.h Sciences (UFR/SDS) Joseph Ki Zerbo University Ouagadougou Burkina Faso Department of Odontostomatol.gy and Maxil.ofacial.Surgery Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso Department of Surgery UFR of Odonto-Stomatology Félix Houphou&euml t-Boigny University of Abidjan Abidjan Republic of C&ocirc te d’Ivoire Department of Odontostomatol.gy and Maxil.ofacial.Surgery Bogodogo University Hospital Center Ouagadougou Burkina Faso

Background: Cervicofacial.cel.ul.tis is a severe infection and is responsibl. for a major disruption of host homeostasis. The aim of this work was to describe the haematol.gical.and biochemical.variations of cervico-facial.cel.ul.tis. Methods: This was a cross-sectional.study conducted from 1 January to 31 December 2020. Al. patients hospital.zed for cervico-facial.cel.ul.tis of dental.origin were incl.ded in the study. The usual.parameters of descriptive statistics were estimated for each variabl.. Resul.s: our sampl. consisted of 166 patients. The average age of patients with cervicofacial.cel.ul.tis in our study was 39.9 ± 15.39 years. Red bl.od cel. count was l.w in 55.5% of patients and hyperl.ukocytosis was noted in 89.16% of patients. Pl.tel.ts were normal.in 43.14% of patients and 78.43% of patients had a l.w haematocrit (haemodil.tion). Anemia was noted in 64.7% of patients. In addition, 61.0% of patients had el.vated uricemia and 31.17% had el.vated creatinemia. Concl.sion: Cel.ul.tis is stil. a common condition in l.ss privil.ged environments. Its diagnosis can be guided by cl.nical.and biol.gical.findings. Consistent education of the popul.tion on oral.heal.h, and the control.of biol.gical.disorders that resul. from it, are sufficient to greatl. reduce its preval.nce and the appearance of compl.cated forms.

关键词： Cervicofacial.Cel.ul.tis Teeth Haematol.gical.Variations Biochemical.Variations ouagadougou

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Treatment of Traumatic Acetabul.m l.sions in an African Orthopaedic Trauma Department

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Open Journal.of Orthopedics 2019年第10期9卷 197-211页

作者： S. C. Da S. A. Korsaga Aji ouedraogo C. Darga H. Kafando M. O. Somé S. Tinto D. Denné l. ouédraogo M. Sawadogo Department of Orthopedics-Traumatol.gy University Hospital Yalgado Ouedraogo Ouagadougou Burkina Faso

Background: The management of traumatic acetabul.r injuries (TAI), which are often compl.x and diverse, is difficul. and costl. in the context of l.w-income African countries. Objective: To eval.ate the treatment of traumatic acetabul.r l.sions in the Orthopedics and Traumatol.gy Department of the Yal.ado ouedraogo University Hospital. for their better management. Patients and Methods: This was a retrospective study, conducted in our department from January 2012 to December 2016. Sixty-three patients with TAI and compl.te records were sel.cted. The mean age of patients with coxofemoral.disl.cations was 34.2 years and 36.4 years for acetabul.m fractures with mal. predominance in both injury types. The injuries were mainl. caused by a viol.nt road traffic accident (RTA) (90.5%). Forty hip disl.cations and 41 acetabul.r fractures were reported, with a preval.nce of il.ac disl.cations (52.5%) and posterior wal. fractures of the acetabul.m (24.4%). Resul.s: The average time to manage TAI was 15.9 hours (range 2 - 100). Medical.treatment was performed in al. patients. Thirty-eight coxofemoral.disl.cations and 34 acetabul.r fractures were treated by orthopedic methods. Seven compl.x acetabul.r fractures and two coxo-femoral.disl.cations were performed by surgical.method. Two patients died (3.2%), one in a hemorrhagic shock tabl. and the other in a septic shock tabl.. Immediate and l.te compl.cations were identified. Concl.sion: Earl. and adequate management of our TAI, requires a modern technical.pl.tform and a sufficient number of qual.fied medical.personnel.to improve their functional.outcomes.

关键词： Hip Acetabul.m Disl.cation Fracture Treatment

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Petrographic and Geochemical.Characteristics of the Granitoids in the South of Godé (North of the Square Degree of l.o-Burkina Faso, West Africa)

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International.Journal.of Geosciences 2024年第2期15卷 106-118页

作者： Adama ouédraogo Yaméogo Hubert Gounwendmanaghré Zongo Pascal.ouiya Omar Akonyiré Abraham Seydoux Traoré Saga Sawadogo Séta Naba Unité de Formation et de Recherche en Sciences et Technol.gies Département des Sciences de la Vie et de la Terre Université Norbert Zongo Koudougou Burkina Faso Unité de Formation et de Recherche en Sciences de l. Vie et de l. Terre Laboratoire Géosciences et Environnement (LaGE) Départe-ment des Sciences de la Terre Université Joseph Ki-Zerbo Ouaga Burkina Faso Ecol. Supérieure d’Ingénieries Université de Fada N’gourma Fada N’Gourma Burkina Faso Ecol. Normal. Supérieure (ENS)/Institut des Sciences et de Technol.gie (IST) Ouaga Burkina Faso

South of Godé, in the central.western region of Burkina Faso, granitoids of Pal.oproterozoic age are simil.r to those of the Man/l.o shiel.. This study focused on the petrographic and geochemical.characteristics of these granitoids, with the fol.owing resul.s: 1) The tonal.te that outcrops in the south-west of the study area bel.ngs to the TTG group or first generation granitoids. They are most often ribboned at outcrop and have a geochemical.signature cl.se to that of Archean TTGs. Tonal.te has a metal.minous character and the REE spectrum indicates that it may be derived from partial.mel.ing of basic magmatic rocks. 2) Biotite granites have no outcrop structure. They are weakl. metal.minous to peral.minous and potassic to highl. potassic. Their rare earth spectra indicate that they may be derived from the partial.mel.ing of TTG granitoids. 3) Geotectonic diagrams show that the granitoids studied to the south of Godé were empl.ced in an active tectonic context simil.r to that of present-day subduction zones.

关键词： Burkina Faso Godé Man/l.o Shiel. Petrography Geochemistry Partial.Mel.ing Geotectonic Setting

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HPV/HBV or HPV/HCV Co-Infections in Women Treated for Chronic Hepatitis at Hôpital.Saint Camil.e in ouagadougou, Burkina Faso

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American Journal.of Mol.cul.r Biol.gy 2024年第1期14卷 1-12页

作者： Estel.0} Théodora Mahoukèdè Zohoncon Bagora Bayal. Prosper Bado Rose P. Cl.mence Da Rogomenoma Al.ce ouedraogo Ina Marie Angèl. Traoré Punya Akouél. Kuassi-Kpede Samiratou ouédraogo Essi Etonam Dovo l.ssina Traoré Al.ert Théophane Yonl. Fl.rencia Wendkuuni Djigma Ol.a Mél.nie l.mpo Jacques Simpore l.boratory of Mol.cul.r Biol.gy and Genetics (l.BIOGENE) University Joseph KI-ZERBO Ouagadougou Burkina Faso Centre of Biomol.cul.r Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Hô spital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Facul.y of Heal.h University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Ecol. Normal. Supérieure Koudougou Burkina Faso Chair “Research and Action Against Cancer” Département de Santé Publique Training and Research Unit in Health Sciences University Joseph KI-ZERBO Ouagadougou Burkina Faso Observatoire National.de l. Santé de l. Popul.tion Institut National de Santé Publique Ouagadougou Burkina Faso The Department of Epidemiol.gy Biostatistics and Occupational Health School of Population and Global Health Montreal Canada l.boratory of Pathol.gical.Anatomy and Cytol.gy University Joseph KI-ZERBO Ouagadougou Burkina Faso

Introduction: Cervical.cancer is a publ.c heal.h concern and is mainl. caused by Human papil.omaviruses (HPV). In many parts of the worl., studies are being carried out to understand the different genotypes to better tackl. this issue. We conducted a study to determine the preval.nce of HPV genotypes in women with chronic hepatitis B or C infection, co-infected or not with HIV, treated at the Hôpital.Saint Camil.e in ouagadougou (Burkina Faso). Methods: This study was conducted from April.to Jul. 2023, incl.ding 100 women in gastroenterol.gy at Hôpital.Saint Camil.e. A questionnaire on their socio-demographic and l.fe styl. was administrated;and endocervical.sampl.s were col.ected using steril. swabs and then sent to Centre of Biomol.cul.r Research Pietro Annigoni (CERBA). HPV mol.cul.r detection and genotyping were performed by PCR and hybridization using the HPV Direct Fl.w Chips kit. Data were anal.sis using chi square test or Fischer’s exact test with a significance threshol. for p Resul.s: The preval.nce of HPV infection was 28% (28/100) on the sampl. of women tested. The most frequent genotypes were HPV 52 (8.33%), fol.owed by HPV 18 and 68 (6.25% each) for high-risk HPVs, and HPV 6, 44/55 and 62/81 (8.33% each) for l.w-risk HPVs. Concl.sion: This study, the first of its kind in Burkina Faso on this group of the popul.tion, reveal. that the most frequent genotypes found in this study are not incl.ded in the vaccine avail.bl. in Burkina Faso (Gardasil.sup>®4).

关键词： Human Papil.omavirus Cervical.Cancer Hepatitis Human Immunodeficiency Virus Burkina Faso

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Insight from l.thostructural.and Aeromagnetic Data from the l.o Square Degree, Central.Western Burkina Faso

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Open Journal.of Geol.gy 2024年第1期14卷 19-28页

作者： Adama ouédraogo Yaméogo Pascal.ouiya Gounwendmanaghré Hubert Zongo Omar Akonyiré Abraham Seydoux Traoré Saga Sawadogo Séta Naba Unité de Formation et de Recherche en Sciences et Technol.gies Département des Sciences de la Vie et de la Terre Université Norbert Zongo Koudougou Burkina Faso Unité de Formation et de Recherche en Sciences de l. Vie et de l. Terre Laboratoire Géosciences et Environnement (LaGE) Département des Sciences de la Terre Université Joseph Ki-Zerbo Ouaga Burkina Faso Ecol. Normal. Supérieure (ENS)/Institut des Sciences et de Technol.gie (IST) Ouaga Burkina Faso Ecol. Supérieure d’Ingénieries Université de Fada N’gourma Fada N’Gourma Burkina Faso

This study, carried out in the l.o square degree (west-central.Burkina Faso) in the Pal.eoproterozoic domain of the Man/l.o ridge, aims to define the shapes and positions of granitic pl.tons and the organisation of deformation structures using aeromagnetic data. These data have shown that there are smal. sub-circul.r granitic bodies to the north of the granitic masses in this region, and a l.rge sub-circul.r granitic body to the south, around which are other smal.er granitic bodies. The l.neament map shows that the deformation structures are organised al.ng three main directions and l.rgel. form these sub-circul.r pl.tonic bodies. We suggest that the granitic pl.tons are coal.scent, pending identification of the internal.structures of these granites to further refine the geodynamic model.

关键词： Burkina Faso l.neaments Bel.s Rocks l.o’s l.af Granitoïd Airbornes Magnétics

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Petrographic and Geochemical.Characteristics of the Pouni Pal.eoproterozoic Formations North of the l.o Square Degree (Burkina Faso, West Africa)

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Open Journal.of Geol.gy 2024年第1期14卷 126-141页

作者： Adama ouédraogo Yaméogo Pascal.ouiya Hubert Gounwendmanaghré Zongo Omar Akonyiré Abraham Seydoux Traoré Saga Sawadogo Séta Naba Unité de Formation et de Recherche en Sciences et Technol.gies Département des Sciences de la Vie et de la Terre Université Norbert Zongo Koudougou Burkina Faso Unité de Formation et de Recherche en Sciences de l. Vie et de l. Terre Laboratoire Géosciences et Environnement (LaGE) Département des Sciences de la Terre Université Joseph Ki-Zerbo Ouaga Burkina Faso Ecol. Normal. Supérieure (ENS) Institut des Sciences et de Technologie (IST) Ouaga Burkina Faso Ecol. Supérieure d’Ingénieries Université de Fada N’gourma Fada N’Gourma Burkina Faso

The Pouni area is made up of basal.s bel.nging to the Boromo bel., l.mprophyres and granitoids. These geol.gical.formations are simil.r to geol.gical.formations of the same type in other regions of the Pal.eoproterozoic domain of the Man/l.o shiel.. This study, which focused on the petrographic and geochemical.characteristics of these geol.gical.formations, l.d to the fol.owing main concl.sions: The l.mprophyres are basic pl.tonic rocks that cut through other geol.gical.formations. The basal. bel.ngs to the northern part of the Borormo bel. and is thought to be a rel.c of overthickened oceanic pl.teaus. There are two groups of granitoid rocks. The granodiorite has a geochemical.signature cl.se to that of Archean TTGs and is metal.minous in character. It has a l.w potassium content. The minor el.ment and rare earth el.ment spectra indicate that it coul. be derived from partial.mel.ing of basic magmatic rocks. Biotite granites are peral.minous and highl. potassic. Minor el.ment contents and rare earth spectra indicate that they coul. be derived from partial.mel.ing of fel.ic material.. Geotectonic diagrams show that the granitoids identified in the Pouni zone were empl.ced in an active tectonic context, simil.r to that of present-day subduction zones.

关键词： Burkina Faso Dori Man/l.o Shiel. Petrography Geochemistry Partial.Mel.ing Geotectonic Context

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Reactions of Maruca Resistant Transgenic Cowpea to Cowpea Aphid-Borne Mosaic Virus

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Agricul.ural.Sciences 2024年第7期15卷 742-753页

作者： Mounyratou Rabo Moustapha ouédraogo Orokia Coul.bal. Assita Traoré-Barro Sal.mata Traoré Teyioue Benoit Joseph Batieno Chantal.Kaboré-Zoungrana Aboubacar Toguyeni oumar Traoré l.boratoire National.de Bioscurit Agence Nationale de Bioscurit (ANB) Ouagadougou Burkina Faso Institut de l.nvironnement et Recherches Agricol.s Dpartement Productions Vgtales Ouagadougou Burkina Faso l.boratoire dEtudes et de Recherches sur l.s Ressources Naturel.es et l.s Sciences de l.nvironnement (l.RNSE) Universit Nazi BONI Bobo-Dioulasso Burkina Faso l.boratoire De Gntique et Aml.oration Des Pl.ntes (l.GAMEP) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso l.boratoire De Protection et Dfense Des Cul.ures (l.PRODEC) Institut de lEnvironnement et Recherches Agricoles Ouagadougou Burkina Faso

Cowpea (Vigna unguicul.ta l. [Wal..]) in one of the main grain l.gumes contributing to food security and poverty al.eviation in Sub-Saharan Africa. To control.the highl. damaging l.gume pod borer Maruca vitrata F., transgenic cowpea l.nes expressing the insecticidal.Cry1Ab Bt protein were devel.ped. In this study, we eval.ated the impact of Cry1Ab transgene expression on the susceptibil.ty of four cowpea l.nes (named IT97K-T, IT98K-T, Gourgou-T and Nafi-T) and their respective non-transgenic near isogenic l.nes (IT97K, IT98K, Gourgou and Nafi) to Cowpea aphid-borne mosaic virus (CABMV) in greenhouse conditions. In a prel.minary qual.ty control.test by enzyme-l.nked immunosorbent assay, the presence of Cry1Ab protein in transgenic seed l.ts ranged from 59% to 72%, with no significant differences among the l.nes (χ2 = 3.26;p = 0.35). Upon virus inocul.tion, al. cowpea l.nes exhibited mosaic symptoms with simil.r severity between 7- and 11-day post-inocul.tion. No significant differences were observed in symptom severity. Significant differences were found between cowpea l.nes for time of symptom onset, virus accumul.tion in pl.nts and days to 50% fl.wering. However, whil. comparing pairs of transgenic l.nes and corresponding non-transgenic l.nes, virus accumul.tion showed not significant differences whatever the pair. Time of symptom onset and days to 50% fl.wering did not al.o differ significantl. between pairs of cowpea l.nes except Nafi/Nafi-T in which transgenic Nafi-T showed earl.er symptoms (7.4 ± 0.7 vs. 8.9 ± 0.8 days post-inocul.tion) and shorter fl.wering time (37.3 ± 0.6 vs. 42 ± 1.7 days after sowing). Overal., these findings improve our understanding of the effects of Cry1Ab gene mediated genetic modification on cowpea infection by Cowpea aphid-borne mosaic virus, with potential.impl.cations for environmental.safety assessment.

关键词： Cowpea Cry1Ab Cowpea Aphid-Borne Mosaic Virus Environmental.Safety

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Symphyseal.Disjunction after Dystocic Vaginal.Del.very: About Two Cases at the SourôSanou University Hospital.of Bobo-Dioul.sso (CHUSS)

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Open Journal.of Obstetrics and Gynecol.gy 2024年第1期14卷 1-6页

作者： Mil.ogo Jean De l. Croix Sanou Jean de Dieu Gadigbé Akofa Méré Godé Sabi Tokobou Wil.iam ouédraogo André Togbé Eric Serge Al.honou Yaméogo Rél.endé Barnabé Dembél. Adama Somé Der Adol.he ouattara Soul.ymane Obstetrics-Gynecol.gy and Reproductive Medecine Departement of Sourô Sanou Teaching Hospital Bobo-Dioulasso Burkina Faso Higher Institute of heal.h Sciences (INSSA) Université nazi Boni Bobo-Dioulasso Burkina Faso

Diastasis of the symphysis pubis is a rare postpartum compl.cation. We report two cases of symphysis diastasis after dystocic del.very, in two patients who presented in the postpartum period with functional.impotence, pubal.ia and acute urine retention. A frontal.radiograph of the pel.is confirmed the diagnosis, showing a symphyseal.separation of 12 mm and 18 mm. In our case, management was mainl. medical. with a favorabl. cl.nical.outcome.

关键词： Pel.is Symphyseal.Disjunction/Diastasis Post-Partum Burkina Faso

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