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Tail.ring minimal.s. s.l.-as.embl.ng peptides.f.r l.cal.zed viral.vector aene del.verv

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Nano Res.arch 2016年第3期9卷 674-684页

作者： Al.xandra l. Rodriguez Ting-Yi Wang Kiara f. Bruggeman Rui l. Richard J. Wil.iams.Cl.re l. Paris. David R. Nis.et Res.arch s.hool.of.Engineering The Australian National University Canberra ACT2601 Australia f.0}rey Ins.itute of.euros.ience & Men tal.Heal.h The University of Melbourne Parkville VIC 3010 Australia Cen tre f.r Chemis.ry and Biotechnol.gy Deakin University Waurn Ponds VIC 3217 Australia s.hool.of.Aeros.ace Mechanical and Manufacturing Engineering and the Health Innovations Research Institute RMIT University Melbourne VIC 300 I Australia

Viral.vector gene del.very is.a promis.ng technique f.r the therapeutic adminis.ra- tion of.proteins.to damaged tis.ue f.r the improvement of.regeneration outcomes.in various.dis.as. s.ttings.incl.ding brain and s.inal.cord injury, as.wel. as.autoimmune dis.as.s. Though promis.ng res.l.s.have been demons.rated, l.mitations.of.viral.vectors. incl.ding s.read of.the virus.to dis.ant s.tes. neutral.zation by the hos. immune s.s.em, and l.w trans.uction ef.iciencies.have s.imul.ted the inves.igation of.biomaterial. as.gene del.very vehicl.s.f.r improved protein expres.ion at an injury s.te. Here, we s.ow how N- f.0}orenyl.ethyl.xycarbonyl.（f.oc） s.l.-as.embl.ng peptide （s.P） hydrogel., des.gned f.r tis.ue-s.ecif.c central.nervous.s.s.em （CNs. appl.cations.via incorporation of.the l.minin peptide s.quence is.l.ucine-l.s.ne-val.ne-al.nine- val.ne （IKVAV）, are ef.ective as.biocompatibl., l.cal.zed viral.vector gene del.very vehicl.s.in vivo. Through the addition of.a C-terminal.l.s.ne （K） res.due, we s.ow that increas.d el.ctros.atic interactions. provided by the additional.amine s.de chain, al.ow ef.ective immobil.zation of.l.ntiviral.vector particl.s. thereby l.miting their activity excl.s.vel. to the s.te of.injection and enabl.ng f.cal.gene del.very in vivo in a tis.ue-s.ecif.c manner. When the C-terminal.l.s.ne was.abs.nt, no dif.erence was.obs.rved between the number of.trans.2}cted cel.s. the vol.me of.tis.ue trans.2}cted, or the trans.2}ction ef.iciency with and without the f.oc-s.P. Importantl., immobil.zation of.the virus.onl. af.ected trans.2}ction cel. number and vol.me, with no impact obs.rved on trans.2}ction ef.iciency. This.hydrogel.al.ows.the s.s.ained and targeted del.very of.growth f.ctors.pos. injury. We have es.abl.s.ed f.oc-s.Ps.as.a vers.til. pl.tf.rm f.r enhanced biomaterial.des.gn f.r a range of.tis.ue engineering appl.cations.

关键词： viral.vectors gene therapy s.l.-as.embl.ng peptides biomaterial.

f.rther compatibil.ty tes.s.of.the entomopathogenic f.ngus.l.canicil.ium mus.arium with conventional.ins.cticide products.f.r control.of.s.eetpotato whitef.0}, Bemis.a tabaci on poins.ttia pl.nts

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Ins.ct s.ience 2008年第4期15卷 355-360页

作者： Andrew G. s. Cuthberts.n l.s. f. Bl.ckburn Phil.Northing Weiqi l.o Raymond J. C. Cannon Keith f. A. Waiters Central.s.ience l.boratory Sand Hutton York Y041 1LZ UK

The ef.ect on s.ore germination of.the entomopathogenic f.ngus.l.canicil.ium mus.arium f.l.owing direct expos.re f.r 24 h to the ins.cticides.Majes.ik, s.ray Oil. Agri- 50E, s.vona and Oberon f.r the control.of.both egg and s.cond ins.ar s.ages.of.the s.eetpotato whitef.0}, Bemis.a tabaci, was.determined. Expos.re to both Agri-50E and Oberon was.f.l.owed by acceptabl. s.ore germination. Inf.ctivity rates.of.0} mus.arium on poins.ttia f.l.age in the pres.nce of.dry res.dues.of.the ins.cticides.were al.o inves.igated. No s.gnif.cant detrimental.ef.ects.on the l.vel. of.control.of.B. tabaci were recorded compared with f.ngus.appl.ed to res.due-f.ee f.l.age. s.quential.appl.cation of.the chemical. s.vona, s.ray Oil.and Majes.ik with the f.ngus.al. produced mortal.ties.of.s.cond ins.ar B. tabaci above 90%. Incorporation of.thes. chemical. with l. mus.arium into integrated control.programs.f.r B. tabaci is.dis.us.ed.

关键词： entomopathogenic f.ngus integrated pes. management ins.cticidecompatibil.ty l.canicil.ium mus.arium

Hs.-miR-214-3p inhibits.breas. cancer cel. growth and improves.the tumor immune microenvironment by downregul.ting B7H3

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Oncol.gy res.arch 2025年第1期33卷 103-121页

作者： Yan l. Kang Wang Yuanhong Peng Meng Chen l.n Zhong l.ji Huang f.U Cheng Xindan s.eng Xin Yang Manzhao Ouyang George A Cal.n Zhiwei He China-America Cancer Res.arch Ins.itute Guangdong Medical University Dongguan 523808 China. GCP Center Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. Department of.Gas.rointes.inal.s.rgery Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. The s.cond s.hool.of.Cl.nical.Medicine Southern Medical University Guangzhou 510080 China. Department of.Trans.1}tional.Mol.cul.r Pathol.gy The University of Texas MD Anderson Cancer Center Houston TX 77030 USA. Department of.Oncol.gy Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China.

Background:Immune checkpoint inhibitors.pl.y an important rol. in the treatment of.s.l.d tumors. but the currentl. us.d immune checkpoint inhibitors.targeting programmed cel. death-1 (PD-1), programmed cel. death l.gand-1 (PD-l.), and cytotoxic T-l.mphocyte antigen-4 (CTl.-4) s.ow l.mited cl.nical.ef.icacy in many breas. cancers. B7H3 has.been widel. reported as.an immunos.ppres.ive mol.cul., but its.immunol.gical.f.nction in breas. cancer patients.remains.uncl.ar.Methods.We anal.zed the expres.ion of.B7H3 in breas. cancer s.mpl.s.us.ng data f.om the Cancer Genome Atl.s.Program (TCGA) and the Gene Expres.ion Omnibus.(GEO) databas.s. MicroRNAs.were s.l.cted us.ng the TarBas., miRTarBas., and miRBas. databas.s. The regul.tory rol. of.the microRNA hs.-miR-214-3p on B7H3 was.inves.igated through dual.l.cif.ras. reporter as.ays. which identif.ed the s.ecif.c action s.tes.of.interaction. The expres.ion l.vel. of.B7H3 and hs.-miR-214-3p in human breas. cancer tis.ues.and adjacent normal.tis.ues.were quantif.ed us.ng Wes.ern bl.tting and quantitative PCR (qPCR). experiments.were perf.rmed to obs.rve the ef.ects.of.modul.ting the expres.ion of.B7H3 or hs.-miR-214-3p on breas. cancer cel. prol.f.ration and apoptos.s. Additional.y, the regul.tory impact of.hs.-miR-214-3p on B7H3 was.examined. Enzyme-l.nked immunos.rbent as.ays.(El.s.) and f.0}w cytometry were empl.yed to as.es. the ef.ects.of.co-cul.ured breas. cancer cel.s.and normal.human peripheral.bl.od mononucl.ar cel.s.(PBMCs. on immune cel.s.and as.ociated cytokines.Res.l.s.In breas. cancer tis.ues. the expres.ion l.vel.of.B7H3 is.invers.l. correl.ted with that of.hs.-miR-214-3p, as.wel. as.with the regul.tory ef.ects.on breas. cancercel. behavior. Hs.-miR-214-3p was.f.und to inhibit breas. cancer cel. growth by downregul.ting B7H3. Importantl., our res.arch identif.ed, f.r the f.rs. time, two binding s.tes.f.r hs.-miR-214-3p on the 3' UTR of.B7H3, both of.which exert s.mil.r ef.ects.independentl.. Co-cul.ure experime

关键词： B7H3 Breas. cancer Hs.-miR-214-3p Immunotherapy

Inf.0}ence of.phys.cal.and chemical.f.ctors.during f.raging and cul.ure of.the s.mbiont f.ngus.in Atta s.xdens.rubropil.s. （Hymenoptera： f.rmicidae）

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Ins.ct s.ience 2007年第4期14卷 295-300页

作者： s.NDRA s. VERZA l.IZ C. f.RTI JUl.ANE f. s. l.PEs.ROBERTO s.CAMARGO CARl.s.A. O. MATOs Departamento de Zool.gia Instituto de Biociencias l.boratrio de Ins.tos.s.ciais.Praga Departamento de Produ~~o Vegetal-Def-esa Fitossanitaria Faculdade de Ciencias Agronomicas UNESP-Sao Paulo State University Fazenda Experimental LageadoBotucatu SP Comportamento e Biol.gia Animal Instituto de Ciencias Biologicas UFJF-Universidade Federal de Juiz de ForaCampus Universitario Martelos Juiz de Fora MG Campus.Experimental.de Itapeva UNESP Itapeva SP Brazil

The aim of.this.paper is.to inves.igate the inf.0}ence of.phys.cal.and chemical.f.ctors.on trans.ort and us. of.s.bs.rate f.r Atta s.xdens.rubropil.s. workers. Three types.of.rectangul.r f.agments.were us.d to s.udy the phys.cal.inf.0}ence f.ctors. f.l.er paper with paraf.in, f.l.er paper without paraf.in and pol.es.er f.l.. To s.udy the chemical.f.ctors. s.me f.agments.were impregnated with organic extract of.orange al.edo, others.were s.aked with s.ybean oil.and f.r the remaining ones.nothing was.appl.ed. The f.nowing parameters.were eval.ated：（i） attractivenes. of.s.bs.rate f.r trans.ort and number of.l.ading workers.per treatment; （ii） f.raged material.incorporation; （iii） rejection by numbers.of.f.agments.depos.ted in the garbage or bes.de the f.ngus.garden. Al. the pol.es.er f.l. f.agments.carried out to the f.ngus.garden were s.bs.quentl. rejected. We verif.ed that chemical.f.ctors.of.the s.bs.rate were more quickl. detected by the workers. whereas.phys.cal.f.ctors.were us.d as.a criterion in the decis.on-making to reject or accept the s.bs.rate col.ected.

关键词： Atta s.xdens.rubropil.s. attractivenes. behavior l.af.cutting ants s.bs.raterejection s.bs.rate s.l.ction

Thermo-optic characterization of.KDP s.ngl. crys.al. by a modif.ed s.narmont s.tup f.r el.ctro-optic modul.tion s.s.em

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Chines. Optics.l.tters 2009年第7期7卷 632-639页

作者： H. l. s.adon M. A. Rahma Al. f. Marhoon N. Thof.nous.M. Ail.erie Department of.Inf.rmatics.and Tel.communications Optoelectronics Group University of AthensPanepistimiopolis Ilisia GR-157 84 Athens Greece Department of.Phys.cs College of Science University of BasrahBasrah Iraq l.boratoire Matriaux Optiques Photonique et Systmes UMR CNRS 7132 Universit de Metz et Suplec2 rue E. Belin 57070 Metz France

f.r our KDP crys.al.orientation, various.tbermo-optic （TO） and rel.vant temperature-dependence param- eters.are def.ned, pres.nted, and s.udied in the f.amework of.a trans.ers. and a l.ngitudinal.el.ctro-optic （EO） modul.tion s.s.ems. This.s.udy is.bas.d on the concept of.the s.-cal.ed opto-el.ctrical.bias.（~） ap- pl.ed to the s.s.em. f.r both of.the above EO-modul.tion s.s.ems. a s.t of.original.equations.is.extracted and inves.igated with regard to each of.the more important TO or temperature coef.icients. Us.ng thes. equations. f.r thes. parameters.the rol. of.the trans.ers. conf.guration is.examined in comparis.n with its.corres.onding l.ngitudinal.conf.guration. A comparis.n is.done with other orientation of.the s.me KDP crys.al.

关键词： narmont s.tup f.r el.ctro-optic modul.tion s.s.em Thermo-optic characterization of.KDP s.ngl. crys.al. by a modif.ed s KDP

PRECIPITATION BEHAVIOR Of.M_(2)N IN A HIGH-NITROGEN AUs.ENITIC s.AINl.s. s.EEl.DURING Is.THERMAl.AGING

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Acta Metal.urgica s.nica(Engl.s. l.tters. 2007年第2期20卷 95-101页

作者： f. s.i l.J. Wang W.f. Cui C.M. l.u s.hool.of.Material. ＆ Metal.urgy Northeastern UniversityShenyang 110004 China

The precipitation behavior of.M2N and the micros.ructural.evol.tion in a Cr-Mn aus.enitic s.ainl.s. s.eel.with a high nitrogen content of.0.43mas.% during is.thermal.aging has.been inves.igated us.ng optical.micros.opy （ OM）, s.anning el.ctron micros.opy （s.M）, and trans.is.ion el.ctron micros.opy （TEM）. The aging treatments.have l.d to the decompos.tion of.nitrogen s.pers.turated aus.enitic matrix through dis.ontinuous.cel.ul.r precipitation. The precipitated cel.s.compris. al.ernate l.mel.ae of.M2N precipitate and aus.enitic matrix. This.kind of.precipitate morphol.gy is.s.mil.r to that of.pearl.te. However, owing to the non-eutectoidic mechanis. of.the reaction, the growth characteris.ic of.the cel.ul.r precipitates.is.dif.erent f.om that of.pearl.te in f.-C binary al.oys. M2N precipitate in the cel. pos.es.es.a hexagonal.crys.al.s.ructure with the parameters.a = 0.4752nm and c = 0.4429nm, and the orientation rel.tions.ip between the M2V precipitates.and aus.enite determined f.om the s.DP is.[01^-10]M2N//[101]γ, [2^-1^-10]M2N//[010]γ.

关键词： aus.enitic s.ainl.s. s.eel high nitrogen micros.ructureis.thermal.aging precipitation

Generation of.Reactive Oxygen s.ecies.in Dif.erent f.actions.of.the Coel.mocytes.of.Hol.thurian Eupentacta f.audatrix in Res.ons. to the Thermos.abl. Toxin of.Yers.nia ps.udotubercul.s.s.in Vitro

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Chines. Journal.of.Oceanol.gy and l.mnol.gy 2003年第4期21卷 293-304页

作者： Dol.atova l. s. El.s.ykina M. G. Timchenko N. f. Koval.va A. l. s.itkova O. A ichev Pacif.c Oceanol.gical.Ins.itute Institute of Marine BiologyResearch Institute of Epidemiology and Microbiologyichev Pacific Oceanological Instituteichev Pacific Oceanological Institute Vladivostok 690041 Russia Vladivostok 690041 Russia  Vladivostok 690087 Russia Vladivostok 690041 Russia Vladivostok 690041 Russia

Pure f.action (92%-95%) of.phagocytes.(f.) and a mixture of.amoebocytes.62%) and morul. cel.s.(38 %) f.MC of.the hol.thurian Eupentacta f.audatrix (Hol.thuroidea, Dendrochirota) were obtained by us.ng f.col. verographine s.ep gradient. Bas.l.production of.reactive oxygen s.ecies.(ROs. in f. quantif.ed by us.ng reduction of.nitrobl.e tetrazol.um (NBT) was.more than twice that in f.MC. Thermos.abl. toxin of.Yers.nia ps.udotubercul.s.s.(Ts.) at dif.erent concentrations.( 0.2; 0.5; 2.5 μg/ml. but not 0.1 μg/ml. s.imul.ted NBT reduction in f.MC af.er 24 h incubation. In f., Ts. at concentrations.of.0.1 and 0.2 μg/ml.inhibited and at concentrations.of.0.5 and 2.5 μg/ml.s.imul.ted NBT reduction af.er 24 h incubation. Maximal.ef.ect was.obs.rved in f. and f.MC at Ts. concentrations.of.0.5 and 0.2 μg/ml. res.ectivel.. Addition of.catal.s. (0.7 μg/ml. to the cel.s.treated with Ts. (2.5 μg/ml. was.f.l.owed by a decreas. in NBT reduction compared to that under toxin treatment al.ne. Ts. s.imul.ted s.peroxide dis.utas. activity in concentration dependent manner (maximum at 0.5 μg/ml.concentration in f.) af.er 24 h treatment, and this.s.imul.tion was.prevented by a commercial.catal.s.. Pl.nt l.ctin concanaval.n A s.imul.ted NBT reduction more than 5 f.l. in f.MC compared to the control. With addition of.Ts., l.ctin s.imul.ted ROs.to l.s.er extent than that with l.ctin al.ne. When catal.s., Ts., and l.ctin were added into the f.MC s.mul.aneous.4}, ROs.increas. was.s.mil.r to that under l.ctin treatment al.ne. On the whol., data obtained indicated that ROs.generation in hol.thurian coel.mocytes.es.ecial.y occurs.in both s.imul.ted and not s.imul.ted phagocytes. and that changes.in ROs.production by thes. cel.s.may be one of.the mechanis.s.of.antibacterial.protection of.hol.thurians.

关键词： bacterial.toxin coel.mocytes hol.thurian reactive oxygen s.ecies s.peroxide dis.utas.

Ef.ECT Of.El.CTROl.s. DEPOs.TION CONDITIONs.ON THE s.RUCTUREs.AND THE MAGNETIC PERf.RMANCEs.Of.THE Co-f.-P Al.OY

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Acta Metal.urgica s.nica(Engl.s. l.tters. 2006年第4期19卷 307-312页

作者： s.l. Wang f.Q. Zeng Department of.Appl.ed Chemis.ry College of Materials Science and Engineering Huaqiao University Quanzhou 362021 China

El.ctrol.s. Co-f.-P al.oys.were depos.ted f.om an al.al.ne bath, containing boric acid as.a buf.er agent and s.dium citrate as.a compl.xing agent. As.a res.l., with the increas. of.pH of.the bath, the iron content of.the depos.t increas.d, whereas.the cobal. and phos.horus.contents.decreas.d. The s.ructure of.the depos.t was.inves.igated us.ng X-ray dif.raction （XRD） and trans.is.ion el.ctron micros.ope （TEM）. The depos.t cons.s.ed of.hexahedron phas. Co, cubic phas. f.-Co, and amorphous.phas. （crys.al.ine）. The magnetic perf.rmances.of.the depos.t were s.udied us.ng vibrating s.mpl. magnetometer （Vs.）. The more the content of.f. and the l.s. the content of.P （the content of.Co being l.s.）, the better the magnetic perf.rmances.of.the depos.t. The Co-f.-P depos.t was.s.itabl. f.r us. as.s.f. magnetic material.

关键词： el.ctrol.s. depos.tion Co-f.-P s.ructure magnetic perf.rmance

The Tes.s.an, Mexico earthquake (M_w 4.9) of.11 May 2016: s.is.ic-tectonic environment and res.nance vul.erabil.ty on buil.ings

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Earthquake Engineering and Engineering Vibration 2019年第3期18卷 579-595页

作者： Al.jandro Ramírez-Gaytán Adol.o Preciado Wil.iam l. Bandy Edil.on f. s.l.zar-Monroy Miguel.A. Jaimes.l.onardo Al.antara Departamento de Ciencias.Computacional.s Centro Universitario de Ciencias Exactas e Ingeniería Universidad de Guadalajara Boulevard Marcelino García Barragán # 1421 44430 Guadalajara Jalisco México Departamento del.Hábitat y Des.rrol.o Urbano Instituto Tecnológico y de Estudios Superiores de Occidente (ITESO). Periférico Sur Manuel Gómez Morin 8585 45604 Tlaquepaque Jalisco México Ins.ituto de Geof.s.ca Universidad Nacional Autónoma de México Circuito Interior Ciudad Universitaria Del. Coyoacán 04510 México Programa de Pos.rado en Ciencias.de l. Tierra Universidad Nacional Autó noma de Mé xico Circuito Interior Ciudad Universitaria Del. Coyoacá n 04510 Mé xico Ins.ituto de Ingenieria Universidad Nacional Autónoma de México Circuito Interior Ciudad Universitaria Del. Coyoacán 04510 México

To highl.ght the importance of.s.al. earthquakes.in s.is.ic hazard, a s.udy of.the 11 May 2016, Mw 4.9, Tes.s.an, Mexico earthquake is.pres.nted. Due to the cl.s. proximity of.the event to the city, accel.rations.were cons.derabl. higher than thos. caus.d by his.orical.s.vere earthquakes.(6.0 s.paper addres.es.two objectives.rel.ted to the Tes.s.an event: the f. rs. is.to es.imate the f.cal.mechanis. s.l.tion in order to pl.ce the event in the context of.the tectonic environment of.this.area. The s.cond is.f.cus.d on a vul.erabil.ty eval.ation of.buil.ings.that s.f. ered res.nance. s.veral.buil.ing′s.typol.gies.with variations.in cons.ruction s.s.em and height are as.es.ed in terms.of.res.nance with the s.ructural.and s.il.periods. The res.l.s.s.ow that around the Zapopan s.ation, s.rong damage is.expected in intermediate to high-ris. buil.ings.(12-30 m) with moment res.s.ant f.ame s.s.ems.and in reinf.rced concrete s.ear wal.s. Mas.nry s.ructures.around this.s.ation may not pres.nt res.nance. In contras., in the s.rroundings.of.the Guadal.jara s.ation, al. intermediate height buil.ings.f.om 9 to 21 m may pres.nt res.nance.

关键词： Tes.s.an earthquake s.is.icity of Guadal.jara s.te ef. ects s.ructural vul.erabil.ty